Amino acid dipepetide frequency for Sphingomonas sp. WG

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.285AlaAla: 21.285 ± 0.254	1.036AlaCys: 1.036 ± 0.035	7.555AlaAsp: 7.555 ± 0.089	8.152AlaGlu: 8.152 ± 0.117	4.353AlaPhe: 4.353 ± 0.066	12.055AlaGly: 12.055 ± 0.124	2.34AlaHis: 2.34 ± 0.046	6.901AlaIle: 6.901 ± 0.078	3.967AlaLys: 3.967 ± 0.062	14.899AlaLeu: 14.899 ± 0.158	3.934AlaMet: 3.934 ± 0.065	3.178AlaAsn: 3.178 ± 0.062	6.941AlaPro: 6.941 ± 0.098	4.835AlaGln: 4.835 ± 0.071	10.421AlaArg: 10.421 ± 0.13	6.534AlaSer: 6.534 ± 0.077	7.172AlaThr: 7.172 ± 0.091	9.092AlaVal: 9.092 ± 0.09	1.838AlaTrp: 1.838 ± 0.048	2.576AlaTyr: 2.576 ± 0.047	0.0AlaXaa: 0.0 ± 0.0
Cys	0.916CysAla: 0.916 ± 0.026	0.092CysCys: 0.092 ± 0.009	0.494CysAsp: 0.494 ± 0.02	0.337CysGlu: 0.337 ± 0.015	0.276CysPhe: 0.276 ± 0.015	0.77CysGly: 0.77 ± 0.024	0.182CysHis: 0.182 ± 0.012	0.307CysIle: 0.307 ± 0.017	0.148CysLys: 0.148 ± 0.011	0.62CysLeu: 0.62 ± 0.023	0.135CysMet: 0.135 ± 0.011	0.185CysAsn: 0.185 ± 0.012	0.407CysPro: 0.407 ± 0.021	0.172CysGln: 0.172 ± 0.012	0.538CysArg: 0.538 ± 0.024	0.385CysSer: 0.385 ± 0.019	0.418CysThr: 0.418 ± 0.018	0.506CysVal: 0.506 ± 0.022	0.114CysTrp: 0.114 ± 0.009	0.149CysTyr: 0.149 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	8.059AspAla: 8.059 ± 0.096	0.435AspCys: 0.435 ± 0.019	2.924AspAsp: 2.924 ± 0.059	2.859AspGlu: 2.859 ± 0.061	2.067AspPhe: 2.067 ± 0.042	5.436AspGly: 5.436 ± 0.081	1.208AspHis: 1.208 ± 0.035	2.489AspIle: 2.489 ± 0.049	1.516AspLys: 1.516 ± 0.038	5.802AspLeu: 5.802 ± 0.078	1.236AspMet: 1.236 ± 0.036	1.196AspAsn: 1.196 ± 0.035	3.904AspPro: 3.904 ± 0.056	1.888AspGln: 1.888 ± 0.05	4.839AspArg: 4.839 ± 0.07	2.346AspSer: 2.346 ± 0.043	2.554AspThr: 2.554 ± 0.044	4.104AspVal: 4.104 ± 0.062	1.098AspTrp: 1.098 ± 0.033	1.485AspTyr: 1.485 ± 0.037	0.0AspXaa: 0.0 ± 0.0
Glu	7.967GluAla: 7.967 ± 0.124	0.278GluCys: 0.278 ± 0.015	2.578GluAsp: 2.578 ± 0.052	2.684GluGlu: 2.684 ± 0.064	1.384GluPhe: 1.384 ± 0.037	4.453GluGly: 4.453 ± 0.07	1.089GluHis: 1.089 ± 0.033	2.623GluIle: 2.623 ± 0.048	1.79GluLys: 1.79 ± 0.043	4.827GluLeu: 4.827 ± 0.069	1.282GluMet: 1.282 ± 0.035	1.217GluAsn: 1.217 ± 0.031	2.435GluPro: 2.435 ± 0.048	2.146GluGln: 2.146 ± 0.045	4.871GluArg: 4.871 ± 0.075	2.23GluSer: 2.23 ± 0.046	3.002GluThr: 3.002 ± 0.051	3.44GluVal: 3.44 ± 0.062	0.76GluTrp: 0.76 ± 0.026	0.88GluTyr: 0.88 ± 0.023	0.0GluXaa: 0.0 ± 0.0
Phe	4.952PheAla: 4.952 ± 0.071	0.294PheCys: 0.294 ± 0.017	2.702PheAsp: 2.702 ± 0.048	1.958PheGlu: 1.958 ± 0.043	1.282PhePhe: 1.282 ± 0.038	3.635PheGly: 3.635 ± 0.071	0.747PheHis: 0.747 ± 0.027	1.141PheIle: 1.141 ± 0.032	0.766PheLys: 0.766 ± 0.025	3.246PheLeu: 3.246 ± 0.057	0.624PheMet: 0.624 ± 0.024	1.046PheAsn: 1.046 ± 0.035	1.463PhePro: 1.463 ± 0.033	0.997PheGln: 0.997 ± 0.029	2.375PheArg: 2.375 ± 0.046	1.865PheSer: 1.865 ± 0.042	2.137PheThr: 2.137 ± 0.047	2.708PheVal: 2.708 ± 0.048	0.522PheTrp: 0.522 ± 0.023	0.916PheTyr: 0.916 ± 0.033	0.0PheXaa: 0.0 ± 0.0
Gly	10.605GlyAla: 10.605 ± 0.098	0.804GlyCys: 0.804 ± 0.025	4.868GlyAsp: 4.868 ± 0.071	4.714GlyGlu: 4.714 ± 0.066	3.758GlyPhe: 3.758 ± 0.056	8.284GlyGly: 8.284 ± 0.139	1.796GlyHis: 1.796 ± 0.046	4.44GlyIle: 4.44 ± 0.063	3.073GlyLys: 3.073 ± 0.067	8.657GlyLeu: 8.657 ± 0.093	2.359GlyMet: 2.359 ± 0.046	2.294GlyAsn: 2.294 ± 0.049	3.733GlyPro: 3.733 ± 0.068	3.073GlyGln: 3.073 ± 0.046	6.524GlyArg: 6.524 ± 0.077	4.925GlySer: 4.925 ± 0.071	5.301GlyThr: 5.301 ± 0.086	6.477GlyVal: 6.477 ± 0.082	1.757GlyTrp: 1.757 ± 0.047	2.457GlyTyr: 2.457 ± 0.048	0.0GlyXaa: 0.0 ± 0.0
His	2.529HisAla: 2.529 ± 0.047	0.217HisCys: 0.217 ± 0.013	1.153HisAsp: 1.153 ± 0.032	0.927HisGlu: 0.927 ± 0.028	0.782HisPhe: 0.782 ± 0.024	1.996HisGly: 1.996 ± 0.046	0.584HisHis: 0.584 ± 0.024	0.812HisIle: 0.812 ± 0.028	0.402HisLys: 0.402 ± 0.019	1.888HisLeu: 1.888 ± 0.044	0.394HisMet: 0.394 ± 0.02	0.424HisAsn: 0.424 ± 0.02	1.3HisPro: 1.3 ± 0.034	0.574HisGln: 0.574 ± 0.022	1.584HisArg: 1.584 ± 0.044	0.859HisSer: 0.859 ± 0.03	0.678HisThr: 0.678 ± 0.022	1.413HisVal: 1.413 ± 0.036	0.377HisTrp: 0.377 ± 0.019	0.535HisTyr: 0.535 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	7.823IleAla: 7.823 ± 0.087	0.31IleCys: 0.31 ± 0.016	3.819IleAsp: 3.819 ± 0.062	3.303IleGlu: 3.303 ± 0.061	1.351IlePhe: 1.351 ± 0.036	5.201IleGly: 5.201 ± 0.076	0.802IleHis: 0.802 ± 0.027	1.468IleIle: 1.468 ± 0.039	1.004IleLys: 1.004 ± 0.035	3.936IleLeu: 3.936 ± 0.059	0.646IleMet: 0.646 ± 0.023	1.233IleAsn: 1.233 ± 0.039	2.027IlePro: 2.027 ± 0.04	1.216IleGln: 1.216 ± 0.031	3.129IleArg: 3.129 ± 0.05	2.151IleSer: 2.151 ± 0.05	2.497IleThr: 2.497 ± 0.054	4.029IleVal: 4.029 ± 0.065	0.509IleTrp: 0.509 ± 0.02	0.901IleTyr: 0.901 ± 0.028	0.0IleXaa: 0.0 ± 0.0
Lys	3.71LysAla: 3.71 ± 0.067	0.116LysCys: 0.116 ± 0.01	1.34LysAsp: 1.34 ± 0.036	1.031LysGlu: 1.031 ± 0.031	0.758LysPhe: 0.758 ± 0.025	2.478LysGly: 2.478 ± 0.051	0.457LysHis: 0.457 ± 0.022	1.269LysIle: 1.269 ± 0.034	0.873LysLys: 0.873 ± 0.037	3.172LysLeu: 3.172 ± 0.057	0.679LysMet: 0.679 ± 0.025	0.672LysAsn: 0.672 ± 0.023	1.921LysPro: 1.921 ± 0.044	0.827LysGln: 0.827 ± 0.025	2.103LysArg: 2.103 ± 0.053	1.303LysSer: 1.303 ± 0.032	1.6LysThr: 1.6 ± 0.042	2.031LysVal: 2.031 ± 0.048	0.335LysTrp: 0.335 ± 0.018	0.527LysTyr: 0.527 ± 0.024	0.0LysXaa: 0.0 ± 0.0
Leu	15.17LeuAla: 15.17 ± 0.139	0.761LeuCys: 0.761 ± 0.03	6.066LeuAsp: 6.066 ± 0.076	4.672LeuGlu: 4.672 ± 0.078	3.78LeuPhe: 3.78 ± 0.064	8.66LeuGly: 8.66 ± 0.097	1.979LeuHis: 1.979 ± 0.046	4.587LeuIle: 4.587 ± 0.066	2.713LeuLys: 2.713 ± 0.052	10.194LeuLeu: 10.194 ± 0.135	1.953LeuMet: 1.953 ± 0.042	2.376LeuAsn: 2.376 ± 0.05	6.047LeuPro: 6.047 ± 0.082	2.794LeuGln: 2.794 ± 0.048	7.564LeuArg: 7.564 ± 0.086	5.528LeuSer: 5.528 ± 0.071	5.357LeuThr: 5.357 ± 0.06	7.504LeuVal: 7.504 ± 0.093	1.332LeuTrp: 1.332 ± 0.043	2.055LeuTyr: 2.055 ± 0.041	0.0LeuXaa: 0.0 ± 0.0
Met	3.164MetAla: 3.164 ± 0.051	0.136MetCys: 0.136 ± 0.011	1.014MetAsp: 1.014 ± 0.034	0.953MetGlu: 0.953 ± 0.029	0.673MetPhe: 0.673 ± 0.025	1.76MetGly: 1.76 ± 0.037	0.435MetHis: 0.435 ± 0.019	1.231MetIle: 1.231 ± 0.031	0.783MetLys: 0.783 ± 0.025	2.671MetLeu: 2.671 ± 0.047	0.56MetMet: 0.56 ± 0.025	0.619MetAsn: 0.619 ± 0.023	1.424MetPro: 1.424 ± 0.032	0.726MetGln: 0.726 ± 0.023	1.766MetArg: 1.766 ± 0.035	1.21MetSer: 1.21 ± 0.031	1.513MetThr: 1.513 ± 0.038	1.592MetVal: 1.592 ± 0.036	0.202MetTrp: 0.202 ± 0.013	0.266MetTyr: 0.266 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	3.419AsnAla: 3.419 ± 0.054	0.212AsnCys: 0.212 ± 0.013	1.312AsnAsp: 1.312 ± 0.036	1.018AsnGlu: 1.018 ± 0.032	0.939AsnPhe: 0.939 ± 0.031	2.505AsnGly: 2.505 ± 0.056	0.474AsnHis: 0.474 ± 0.02	1.185AsnIle: 1.185 ± 0.036	0.597AsnLys: 0.597 ± 0.022	2.447AsnLeu: 2.447 ± 0.047	0.48AsnMet: 0.48 ± 0.02	0.727AsnAsn: 0.727 ± 0.029	1.731AsnPro: 1.731 ± 0.038	0.835AsnGln: 0.835 ± 0.029	1.798AsnArg: 1.798 ± 0.04	1.164AsnSer: 1.164 ± 0.036	1.213AsnThr: 1.213 ± 0.037	1.848AsnVal: 1.848 ± 0.045	0.439AsnTrp: 0.439 ± 0.021	0.748AsnTyr: 0.748 ± 0.03	0.0AsnXaa: 0.0 ± 0.0
Pro	7.757ProAla: 7.757 ± 0.101	0.318ProCys: 0.318 ± 0.017	3.616ProAsp: 3.616 ± 0.063	3.524ProGlu: 3.524 ± 0.059	1.953ProPhe: 1.953 ± 0.043	4.993ProGly: 4.993 ± 0.073	1.059ProHis: 1.059 ± 0.032	2.557ProIle: 2.557 ± 0.047	1.444ProLys: 1.444 ± 0.039	5.132ProLeu: 5.132 ± 0.072	1.168ProMet: 1.168 ± 0.033	1.373ProAsn: 1.373 ± 0.032	2.824ProPro: 2.824 ± 0.068	1.806ProGln: 1.806 ± 0.045	3.295ProArg: 3.295 ± 0.053	2.834ProSer: 2.834 ± 0.049	2.586ProThr: 2.586 ± 0.054	4.348ProVal: 4.348 ± 0.058	0.741ProTrp: 0.741 ± 0.029	1.172ProTyr: 1.172 ± 0.032	0.0ProXaa: 0.0 ± 0.0
Gln	4.519GlnAla: 4.519 ± 0.057	0.188GlnCys: 0.188 ± 0.014	1.511GlnAsp: 1.511 ± 0.041	1.312GlnGlu: 1.312 ± 0.037	1.164GlnPhe: 1.164 ± 0.034	2.744GlnGly: 2.744 ± 0.042	0.614GlnHis: 0.614 ± 0.027	1.653GlnIle: 1.653 ± 0.035	0.841GlnLys: 0.841 ± 0.029	3.363GlnLeu: 3.363 ± 0.062	0.793GlnMet: 0.793 ± 0.027	0.783GlnAsn: 0.783 ± 0.033	1.948GlnPro: 1.948 ± 0.046	1.312GlnGln: 1.312 ± 0.043	2.773GlnArg: 2.773 ± 0.054	1.661GlnSer: 1.661 ± 0.034	1.705GlnThr: 1.705 ± 0.04	2.487GlnVal: 2.487 ± 0.044	0.46GlnTrp: 0.46 ± 0.019	0.643GlnTyr: 0.643 ± 0.023	0.0GlnXaa: 0.0 ± 0.0
Arg	9.553ArgAla: 9.553 ± 0.116	0.468ArgCys: 0.468 ± 0.021	4.315ArgAsp: 4.315 ± 0.064	4.0ArgGlu: 4.0 ± 0.068	3.324ArgPhe: 3.324 ± 0.056	5.617ArgGly: 5.617 ± 0.068	1.645ArgHis: 1.645 ± 0.036	4.194ArgIle: 4.194 ± 0.063	1.846ArgLys: 1.846 ± 0.047	8.357ArgLeu: 8.357 ± 0.098	2.02ArgMet: 2.02 ± 0.045	1.858ArgAsn: 1.858 ± 0.043	3.858ArgPro: 3.858 ± 0.065	2.483ArgGln: 2.483 ± 0.056	5.995ArgArg: 5.995 ± 0.09	3.752ArgSer: 3.752 ± 0.053	3.868ArgThr: 3.868 ± 0.063	5.313ArgVal: 5.313 ± 0.066	1.364ArgTrp: 1.364 ± 0.035	2.001ArgTyr: 2.001 ± 0.042	0.0ArgXaa: 0.0 ± 0.0
Ser	6.488SerAla: 6.488 ± 0.068	0.335SerCys: 0.335 ± 0.019	2.797SerAsp: 2.797 ± 0.055	2.296SerGlu: 2.296 ± 0.045	2.164SerPhe: 2.164 ± 0.052	5.293SerGly: 5.293 ± 0.085	0.881SerHis: 0.881 ± 0.029	2.563SerIle: 2.563 ± 0.048	1.279SerLys: 1.279 ± 0.038	4.8SerLeu: 4.8 ± 0.063	1.099SerMet: 1.099 ± 0.029	1.409SerAsn: 1.409 ± 0.034	2.802SerPro: 2.802 ± 0.048	1.456SerGln: 1.456 ± 0.037	3.46SerArg: 3.46 ± 0.058	2.542SerSer: 2.542 ± 0.055	2.699SerThr: 2.699 ± 0.055	3.521SerVal: 3.521 ± 0.059	0.809SerTrp: 0.809 ± 0.027	1.396SerTyr: 1.396 ± 0.036	0.0SerXaa: 0.0 ± 0.0
Thr	6.787ThrAla: 6.787 ± 0.085	0.361ThrCys: 0.361 ± 0.016	2.809ThrAsp: 2.809 ± 0.049	2.248ThrGlu: 2.248 ± 0.049	1.756ThrPhe: 1.756 ± 0.045	5.434ThrGly: 5.434 ± 0.069	0.946ThrHis: 0.946 ± 0.03	3.094ThrIle: 3.094 ± 0.053	1.246ThrLys: 1.246 ± 0.034	6.157ThrLeu: 6.157 ± 0.078	1.154ThrMet: 1.154 ± 0.027	1.405ThrAsn: 1.405 ± 0.041	3.6ThrPro: 3.6 ± 0.062	1.584ThrGln: 1.584 ± 0.037	3.66ThrArg: 3.66 ± 0.054	2.774ThrSer: 2.774 ± 0.054	2.865ThrThr: 2.865 ± 0.059	4.201ThrVal: 4.201 ± 0.075	0.583ThrTrp: 0.583 ± 0.023	1.177ThrTyr: 1.177 ± 0.033	0.0ThrXaa: 0.0 ± 0.0
Val	10.02ValAla: 10.02 ± 0.097	0.477ValCys: 0.477 ± 0.018	4.321ValAsp: 4.321 ± 0.063	4.325ValGlu: 4.325 ± 0.061	2.182ValPhe: 2.182 ± 0.044	5.38ValGly: 5.38 ± 0.085	1.347ValHis: 1.347 ± 0.039	3.385ValIle: 3.385 ± 0.055	1.925ValLys: 1.925 ± 0.045	7.115ValLeu: 7.115 ± 0.088	1.449ValMet: 1.449 ± 0.036	1.995ValAsn: 1.995 ± 0.051	4.328ValPro: 4.328 ± 0.057	2.344ValGln: 2.344 ± 0.044	5.602ValArg: 5.602 ± 0.077	3.897ValSer: 3.897 ± 0.062	4.7ValThr: 4.7 ± 0.059	5.438ValVal: 5.438 ± 0.081	0.855ValTrp: 0.855 ± 0.03	1.41ValTyr: 1.41 ± 0.034	0.0ValXaa: 0.0 ± 0.0
Trp	1.453TrpAla: 1.453 ± 0.045	0.12TrpCys: 0.12 ± 0.01	0.751TrpAsp: 0.751 ± 0.029	0.618TrpGlu: 0.618 ± 0.024	0.578TrpPhe: 0.578 ± 0.021	1.024TrpGly: 1.024 ± 0.03	0.359TrpHis: 0.359 ± 0.017	0.708TrpIle: 0.708 ± 0.023	0.456TrpLys: 0.456 ± 0.02	1.711TrpLeu: 1.711 ± 0.042	0.376TrpMet: 0.376 ± 0.017	0.485TrpAsn: 0.485 ± 0.021	0.721TrpPro: 0.721 ± 0.025	0.703TrpGln: 0.703 ± 0.025	1.427TrpArg: 1.427 ± 0.039	0.917TrpSer: 0.917 ± 0.03	0.84TrpThr: 0.84 ± 0.026	0.894TrpVal: 0.894 ± 0.028	0.303TrpTrp: 0.303 ± 0.018	0.335TrpTyr: 0.335 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.887TyrAla: 2.887 ± 0.04	0.192TyrCys: 0.192 ± 0.013	1.5TyrAsp: 1.5 ± 0.039	1.005TyrGlu: 1.005 ± 0.033	0.851TyrPhe: 0.851 ± 0.03	2.183TyrGly: 2.183 ± 0.05	0.483TyrHis: 0.483 ± 0.021	0.749TyrIle: 0.749 ± 0.024	0.536TyrLys: 0.536 ± 0.023	2.1TyrLeu: 2.1 ± 0.047	0.356TyrMet: 0.356 ± 0.018	0.615TyrAsn: 0.615 ± 0.03	1.088TyrPro: 1.088 ± 0.029	0.705TyrGln: 0.705 ± 0.029	2.105TyrArg: 2.105 ± 0.049	1.211TyrSer: 1.211 ± 0.037	1.136TyrThr: 1.136 ± 0.031	1.549TyrVal: 1.549 ± 0.034	0.379TyrTrp: 0.379 ± 0.019	0.624TyrTyr: 0.624 ± 0.027	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3630 proteins (1189672 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---