Candidatus Coxiella mudrowiae
Average proteome isoelectric point is 7.33
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 822 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0H4UXP7|A0A0H4UXP7_9COXI Coenzyme A biosynthesis bifunctional protein CoaBC OS=Candidatus Coxiella mudrowiae OX=2054173 GN=coaBC PE=3 SV=1
MM1 pKa = 7.08 TFIVIDD7 pKa = 3.24 NCIRR11 pKa = 11.84 CKK13 pKa = 10.25 YY14 pKa = 7.51 TDD16 pKa = 3.93 CVEE19 pKa = 4.15 VCPVDD24 pKa = 4.28 CFHH27 pKa = 7.48 EE28 pKa = 5.32 GPNMLVIDD36 pKa = 4.61 PDD38 pKa = 3.57 EE39 pKa = 5.96 CIDD42 pKa = 4.49 CNLCVPEE49 pKa = 4.98 CPVDD53 pKa = 4.65 AIFAEE58 pKa = 4.79 DD59 pKa = 4.47 DD60 pKa = 3.84 LPEE63 pKa = 4.32 EE64 pKa = 4.32 KK65 pKa = 10.4 QSFLKK70 pKa = 10.98 LNAEE74 pKa = 4.77 LAKK77 pKa = 9.85 QWPIITAKK85 pKa = 10.58 KK86 pKa = 8.98 EE87 pKa = 4.06 APEE90 pKa = 5.34 DD91 pKa = 3.84 ADD93 pKa = 4.52 DD94 pKa = 4.67 WADD97 pKa = 3.76 TPDD100 pKa = 3.26 KK101 pKa = 10.82 LQYY104 pKa = 10.56 LEE106 pKa = 5.36 HH107 pKa = 6.7 EE108 pKa = 4.34 WSEE111 pKa = 4.12
Molecular weight: 12.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.773
IPC2_protein 3.923
IPC_protein 3.884
Toseland 3.681
ProMoST 3.999
Dawson 3.859
Bjellqvist 4.062
Wikipedia 3.783
Rodwell 3.706
Grimsley 3.592
Solomon 3.846
Lehninger 3.808
Nozaki 3.973
DTASelect 4.177
Thurlkill 3.732
EMBOSS 3.795
Sillero 3.999
Patrickios 0.744
IPC_peptide 3.846
IPC2_peptide 3.986
IPC2.peptide.svr19 3.907
Protein with the highest isoelectric point:
>tr|A0A0H4USB9|A0A0H4USB9_9COXI ATP synthase subunit beta OS=Candidatus Coxiella mudrowiae OX=2054173 GN=atpD PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.28 QPSKK9 pKa = 9.83 LKK11 pKa = 10.65 RR12 pKa = 11.84 NRR14 pKa = 11.84 THH16 pKa = 7.33 GFRR19 pKa = 11.84 TRR21 pKa = 11.84 MAIKK25 pKa = 10.42 NGRR28 pKa = 11.84 QVLNRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.8 GRR39 pKa = 11.84 KK40 pKa = 8.55 HH41 pKa = 6.22 LVVV44 pKa = 3.63
Molecular weight: 5.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.413
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.34
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.076
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.034
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
822
0
822
255510
40
1617
310.8
34.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.611 ± 0.072
1.077 ± 0.032
4.512 ± 0.057
6.381 ± 0.101
4.226 ± 0.071
6.601 ± 0.07
2.401 ± 0.035
7.856 ± 0.068
6.284 ± 0.073
10.818 ± 0.106
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.361 ± 0.036
4.204 ± 0.049
4.203 ± 0.042
4.217 ± 0.064
5.412 ± 0.067
5.849 ± 0.054
5.075 ± 0.055
6.586 ± 0.072
1.115 ± 0.034
3.21 ± 0.047
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here