Lake Sarah-associated circular virus-22

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140AQM3|A0A140AQM3_9VIRU Replication associated protein OS=Lake Sarah-associated circular virus-22 OX=1685749 PE=4 SV=1
MM1 pKa = 7.62SEE3 pKa = 5.24RR4 pKa = 11.84ATCWSVTINNPIPADD19 pKa = 3.79EE20 pKa = 5.26DD21 pKa = 3.76NIAQANQRR29 pKa = 11.84SGWKK33 pKa = 10.09VEE35 pKa = 4.07GQLEE39 pKa = 4.12QGEE42 pKa = 4.87NGTQHH47 pKa = 5.69YY48 pKa = 10.38QLMVKK53 pKa = 9.09TPQIRR58 pKa = 11.84FSQIKK63 pKa = 9.0KK64 pKa = 8.62QFPRR68 pKa = 11.84AHH70 pKa = 6.34IEE72 pKa = 3.6VARR75 pKa = 11.84NVKK78 pKa = 9.55ALKK81 pKa = 10.33EE82 pKa = 4.0YY83 pKa = 9.39VHH85 pKa = 7.44KK86 pKa = 10.72DD87 pKa = 3.04DD88 pKa = 4.39TKK90 pKa = 10.61IGEE93 pKa = 4.06IPVNDD98 pKa = 4.69KK99 pKa = 10.95YY100 pKa = 10.93PTMTAFWEE108 pKa = 4.67LIYY111 pKa = 10.45DD112 pKa = 3.31IHH114 pKa = 6.3EE115 pKa = 4.02QLKK118 pKa = 10.55RR119 pKa = 11.84DD120 pKa = 3.38IDD122 pKa = 3.8YY123 pKa = 10.38MGIEE127 pKa = 4.29TPEE130 pKa = 3.88EE131 pKa = 3.88RR132 pKa = 11.84LVLLDD137 pKa = 3.18QCVNLLICQTYY148 pKa = 9.11NVEE151 pKa = 4.33TFGVNPQVRR160 pKa = 11.84SAAKK164 pKa = 9.73KK165 pKa = 8.8YY166 pKa = 10.68LPALFIRR173 pKa = 11.84VEE175 pKa = 3.73KK176 pKa = 10.04MRR178 pKa = 11.84RR179 pKa = 11.84QKK181 pKa = 8.71TVRR184 pKa = 11.84QTEE187 pKa = 4.17LNSAVEE193 pKa = 4.01QNEE196 pKa = 4.2NEE198 pKa = 4.18EE199 pKa = 4.63STSEE203 pKa = 4.12TQSTCEE209 pKa = 4.01TQSTCEE215 pKa = 4.01TDD217 pKa = 3.41EE218 pKa = 5.04SSQSDD223 pKa = 3.4WNDD226 pKa = 2.68

Molecular weight: 26.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140AQM3|A0A140AQM3_9VIRU Replication associated protein OS=Lake Sarah-associated circular virus-22 OX=1685749 PE=4 SV=1
MM1 pKa = 7.27KK2 pKa = 9.94MKK4 pKa = 10.4RR5 pKa = 11.84APAKK9 pKa = 9.1PRR11 pKa = 11.84APARR15 pKa = 11.84PRR17 pKa = 11.84APARR21 pKa = 11.84RR22 pKa = 11.84TRR24 pKa = 11.84VPRR27 pKa = 11.84AIGMTKK33 pKa = 10.17SGLKK37 pKa = 9.74TYY39 pKa = 9.58TFTFKK44 pKa = 10.42PAPQVFIQAVPTPSSGNTLILSSATGAGTAPLSNGKK80 pKa = 9.21CLTPASSNGLTGFYY94 pKa = 10.76DD95 pKa = 3.67WTCATNFLLADD106 pKa = 4.11LQSAPTYY113 pKa = 10.02TGLFDD118 pKa = 4.03AYY120 pKa = 9.24KK121 pKa = 10.04IKK123 pKa = 10.47KK124 pKa = 7.24ITMNIEE130 pKa = 3.89YY131 pKa = 10.29LNNTSSVNSTGLMPTIYY148 pKa = 10.22AYY150 pKa = 10.61FDD152 pKa = 3.5QDD154 pKa = 3.34DD155 pKa = 4.01SAVPVSVADD164 pKa = 3.18ITRR167 pKa = 11.84RR168 pKa = 11.84QGVKK172 pKa = 10.12KK173 pKa = 10.04FQFGNKK179 pKa = 8.65SKK181 pKa = 9.89TSFSFSLKK189 pKa = 8.54PTVSSALEE197 pKa = 4.31TNSGTLIQAAVQKK210 pKa = 10.64AGWINCTQFAIPHH223 pKa = 5.99YY224 pKa = 8.57GLKK227 pKa = 10.44FAVTDD232 pKa = 4.58LYY234 pKa = 11.39LPGVNAIQQAFRR246 pKa = 11.84INWTYY251 pKa = 9.78VVSYY255 pKa = 10.21RR256 pKa = 11.84SPLRR260 pKa = 11.84NNN262 pKa = 3.27

Molecular weight: 28.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

488

226

262

244.0

27.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.197 ± 1.925

1.639 ± 0.382

4.098 ± 0.808

5.328 ± 3.528

3.893 ± 1.121

4.713 ± 1.077

1.025 ± 0.497

5.533 ± 0.441

6.352 ± 0.105

6.557 ± 0.537

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.049 ± 0.109

5.943 ± 0.168

5.738 ± 1.17

6.148 ± 1.507

5.123 ± 0.125

7.377 ± 1.083

9.221 ± 0.838

5.943 ± 0.463

1.434 ± 0.224

3.689 ± 0.394
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski