Microcella alkaliphila

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microcella

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2621 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0U4WTU3|A0A0U4WTU3_9MICO Membrane protein OS=Microcella alkaliphila OX=279828 GN=MalAC0309_0414 PE=3 SV=1
MM1 pKa = 7.19KK2 pKa = 10.13HH3 pKa = 5.97SKK5 pKa = 8.45RR6 pKa = 11.84TRR8 pKa = 11.84AVLAVTAALSMTALAACAPVAEE30 pKa = 5.02PEE32 pKa = 4.39GQEE35 pKa = 3.63QTLNIAYY42 pKa = 8.53WNYY45 pKa = 10.17GPPAEE50 pKa = 5.11AGNEE54 pKa = 4.06AIANGFMEE62 pKa = 5.39ANPDD66 pKa = 3.57VKK68 pKa = 10.53VTLTPIAGDD77 pKa = 3.14NWGSYY82 pKa = 7.74YY83 pKa = 11.34ANLATLIASGEE94 pKa = 4.14RR95 pKa = 11.84PDD97 pKa = 4.36LAFTASEE104 pKa = 4.57GIKK107 pKa = 10.23FLSEE111 pKa = 3.77NGLVVPINEE120 pKa = 4.27YY121 pKa = 11.02LEE123 pKa = 4.6SDD125 pKa = 3.69PDD127 pKa = 3.43AAAIQDD133 pKa = 4.52DD134 pKa = 4.24IASEE138 pKa = 4.14LLDD141 pKa = 3.73SFAVEE146 pKa = 4.37GNITALPNGWNNMVVYY162 pKa = 10.62YY163 pKa = 8.79NTAVFDD169 pKa = 4.34AAGLPYY175 pKa = 10.17PEE177 pKa = 5.74SDD179 pKa = 3.36WTWEE183 pKa = 4.05EE184 pKa = 3.86FRR186 pKa = 11.84EE187 pKa = 4.08TAAALSADD195 pKa = 3.88TNGDD199 pKa = 3.42GVNDD203 pKa = 3.42QFGFTWASNEE213 pKa = 3.91IFPGILPWVANAGGNLVNDD232 pKa = 4.69DD233 pKa = 3.79VTEE236 pKa = 4.22ATANSAPVVEE246 pKa = 4.84AVSFLKK252 pKa = 11.0GLIDD256 pKa = 5.5DD257 pKa = 6.16GIAPAPSPMGDD268 pKa = 2.72IFTAFQNGNVAMFGAGRR285 pKa = 11.84WPSATFLPAGFTDD298 pKa = 3.69WDD300 pKa = 3.47IQIYY304 pKa = 7.38PTGATYY310 pKa = 8.34QTVAGANGYY319 pKa = 10.64AILTSAANPDD329 pKa = 4.16LAWEE333 pKa = 4.13LQKK336 pKa = 10.24YY337 pKa = 5.24TASAEE342 pKa = 4.25IQDD345 pKa = 4.26SLIGTPEE352 pKa = 3.99APRR355 pKa = 11.84DD356 pKa = 4.14AIPTTRR362 pKa = 11.84STAQKK367 pKa = 8.98TVDD370 pKa = 4.39AGIPPQNGALFYY382 pKa = 11.05GSVDD386 pKa = 3.95DD387 pKa = 5.16YY388 pKa = 11.42PVLSPFPAPAQYY400 pKa = 10.56SEE402 pKa = 4.61YY403 pKa = 9.64EE404 pKa = 3.98ATVLRR409 pKa = 11.84FVQLIFAGEE418 pKa = 4.12VAVEE422 pKa = 4.56DD423 pKa = 5.39GLDD426 pKa = 3.4QLQAEE431 pKa = 4.83LTALISGG438 pKa = 4.21

Molecular weight: 46.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0U4NX59|A0A0U4NX59_9MICO TVP38/TMEM64 family membrane protein OS=Microcella alkaliphila OX=279828 GN=MalAC0309_1958 PE=3 SV=1
MM1 pKa = 7.62KK2 pKa = 10.08VRR4 pKa = 11.84NSIKK8 pKa = 10.54SMKK11 pKa = 9.89KK12 pKa = 8.04MPGSQVVRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84GRR24 pKa = 11.84VFVINKK30 pKa = 7.43QNPRR34 pKa = 11.84FKK36 pKa = 10.8ARR38 pKa = 11.84QGG40 pKa = 3.28

Molecular weight: 4.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2621

0

2621

817268

40

1977

311.8

33.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.662 ± 0.078

0.477 ± 0.01

6.394 ± 0.041

5.812 ± 0.041

2.988 ± 0.03

8.743 ± 0.043

2.049 ± 0.025

4.715 ± 0.036

1.855 ± 0.039

10.047 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.79 ± 0.019

1.97 ± 0.028

5.459 ± 0.039

2.724 ± 0.024

7.605 ± 0.051

5.359 ± 0.031

6.041 ± 0.035

8.996 ± 0.043

1.427 ± 0.02

1.886 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski