Cryptotermes secundus
Average proteome isoelectric point is 6.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 27252 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2J7PQL0|A0A2J7PQL0_9NEOP Uncharacterized protein OS=Cryptotermes secundus OX=105785 GN=B7P43_G05024 PE=3 SV=1
MM1 pKa = 7.11 VHH3 pKa = 7.37 DD4 pKa = 4.99 GAFQFDD10 pKa = 4.52 DD11 pKa = 4.14 VFQGDD16 pKa = 4.11 HH17 pKa = 5.78 VVSDD21 pKa = 3.95 GAFQFDD27 pKa = 5.0 DD28 pKa = 3.91 VVQGDD33 pKa = 3.85 NVVCDD38 pKa = 3.97 GAFQSDD44 pKa = 4.37 DD45 pKa = 3.57 VTHH48 pKa = 6.71 GGVFQFDD55 pKa = 3.92 DD56 pKa = 4.05 VVHH59 pKa = 6.8 GDD61 pKa = 3.63 NVVHH65 pKa = 7.59 DD66 pKa = 4.44 GAFQFDD72 pKa = 4.22 DD73 pKa = 3.71 VVHH76 pKa = 6.44 YY77 pKa = 11.15 GEE79 pKa = 4.65 VVQGGCVGMTDD90 pKa = 3.64 DD91 pKa = 5.11 AAVFSDD97 pKa = 4.62 GFAEE101 pKa = 3.94 NDD103 pKa = 3.78 VIVSGTFHH111 pKa = 6.89 VKK113 pKa = 7.92 TT114 pKa = 4.16
Molecular weight: 12.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.691
IPC2_protein 3.617
IPC_protein 3.643
Toseland 3.401
ProMoST 3.859
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.681
Rodwell 3.465
Grimsley 3.312
Solomon 3.656
Lehninger 3.617
Nozaki 3.795
DTASelect 4.126
Thurlkill 3.49
EMBOSS 3.681
Sillero 3.783
Patrickios 0.947
IPC_peptide 3.643
IPC2_peptide 3.745
IPC2.peptide.svr19 3.704
Protein with the highest isoelectric point:
>tr|A0A2J7QS39|A0A2J7QS39_9NEOP DUF1736 domain-containing protein OS=Cryptotermes secundus OX=105785 GN=B7P43_G09725 PE=4 SV=1
NN1 pKa = 6.95 ATTGRR6 pKa = 11.84 TNATTEE12 pKa = 4.05 RR13 pKa = 11.84 TNATTGRR20 pKa = 11.84 INATTGRR27 pKa = 11.84 TNATTGRR34 pKa = 11.84 INATTGRR41 pKa = 11.84 TNATTGRR48 pKa = 11.84 INATTGRR55 pKa = 11.84 TNATTGRR62 pKa = 11.84 INATTGRR69 pKa = 11.84 TNATTGRR76 pKa = 11.84 INATTGRR83 pKa = 11.84 TNATTGRR90 pKa = 11.84 INATTGRR97 pKa = 11.84 TNATTGRR104 pKa = 11.84 INATTGRR111 pKa = 11.84 TNATTGRR118 pKa = 11.84 INATTGRR125 pKa = 11.84 TNATTGRR132 pKa = 11.84 INATTGRR139 pKa = 11.84 TNATTGRR146 pKa = 11.84 INATTGRR153 pKa = 11.84 TNATTGRR160 pKa = 11.84 INATTGRR167 pKa = 11.84 TNATTGRR174 pKa = 11.84 INATTGRR181 pKa = 11.84 TNATTGRR188 pKa = 11.84 INATTGRR195 pKa = 11.84 TNATTGRR202 pKa = 11.84 INATTGRR209 pKa = 11.84 TNATTGRR216 pKa = 11.84 INATTGRR223 pKa = 11.84 TNATTGRR230 pKa = 11.84 TNAKK234 pKa = 8.23 TDD236 pKa = 3.32 RR237 pKa = 11.84 SSATTGRR244 pKa = 11.84 ATT246 pKa = 3.76
Molecular weight: 24.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.524
IPC2_protein 11.257
IPC_protein 12.866
Toseland 13.027
ProMoST 13.525
Dawson 13.027
Bjellqvist 13.027
Wikipedia 13.51
Rodwell 12.544
Grimsley 13.071
Solomon 13.525
Lehninger 13.422
Nozaki 13.027
DTASelect 13.027
Thurlkill 13.027
EMBOSS 13.525
Sillero 13.027
Patrickios 12.252
IPC_peptide 13.525
IPC2_peptide 12.515
IPC2.peptide.svr19 9.229
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
17779
9473
27252
13199505
35
9899
484.3
54.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.223 ± 0.013
2.093 ± 0.012
5.331 ± 0.011
6.987 ± 0.022
3.546 ± 0.011
5.9 ± 0.017
2.821 ± 0.008
4.951 ± 0.011
6.154 ± 0.019
9.211 ± 0.018
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.343 ± 0.007
4.348 ± 0.011
5.128 ± 0.018
4.562 ± 0.013
5.611 ± 0.015
8.437 ± 0.02
5.759 ± 0.014
6.609 ± 0.012
1.123 ± 0.005
2.824 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here