Amino acid dipepetide frequency for Arthrobacter phage Eileen

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.162AlaAla: 18.162 ± 2.275
0.992AlaCys: 0.992 ± 0.301
8.394AlaAsp: 8.394 ± 0.887
5.952AlaGlu: 5.952 ± 0.688
3.053AlaPhe: 3.053 ± 0.385
12.515AlaGly: 12.515 ± 1.942
2.213AlaHis: 2.213 ± 0.379
4.502AlaIle: 4.502 ± 0.703
5.647AlaLys: 5.647 ± 0.701
10.226AlaLeu: 10.226 ± 1.193
2.366AlaMet: 2.366 ± 0.637
4.274AlaAsn: 4.274 ± 0.822
6.487AlaPro: 6.487 ± 1.511
4.808AlaGln: 4.808 ± 0.771
6.792AlaArg: 6.792 ± 0.863
5.189AlaSer: 5.189 ± 0.588
8.547AlaThr: 8.547 ± 1.067
6.792AlaVal: 6.792 ± 0.933
2.213AlaTrp: 2.213 ± 0.347
2.213AlaTyr: 2.213 ± 0.461
0.0AlaXaa: 0.0 ± 0.0
Cys
0.916CysAla: 0.916 ± 0.364
0.0CysCys: 0.0 ± 0.0
0.458CysAsp: 0.458 ± 0.199
0.687CysGlu: 0.687 ± 0.26
0.153CysPhe: 0.153 ± 0.115
1.297CysGly: 1.297 ± 0.439
0.458CysHis: 0.458 ± 0.227
0.458CysIle: 0.458 ± 0.237
0.382CysLys: 0.382 ± 0.198
0.382CysLeu: 0.382 ± 0.168
0.0CysMet: 0.0 ± 0.0
0.229CysAsn: 0.229 ± 0.131
1.068CysPro: 1.068 ± 0.37
0.382CysGln: 0.382 ± 0.161
0.611CysArg: 0.611 ± 0.277
0.916CysSer: 0.916 ± 0.262
0.763CysThr: 0.763 ± 0.27
0.305CysVal: 0.305 ± 0.144
0.076CysTrp: 0.076 ± 0.08
0.153CysTyr: 0.153 ± 0.115
0.0CysXaa: 0.0 ± 0.0
Asp
7.479AspAla: 7.479 ± 0.857
0.916AspCys: 0.916 ± 0.339
4.045AspAsp: 4.045 ± 0.561
3.129AspGlu: 3.129 ± 0.541
2.06AspPhe: 2.06 ± 0.375
5.952AspGly: 5.952 ± 0.851
1.374AspHis: 1.374 ± 0.383
2.595AspIle: 2.595 ± 0.41
2.976AspLys: 2.976 ± 0.405
4.35AspLeu: 4.35 ± 0.553
1.374AspMet: 1.374 ± 0.309
1.755AspAsn: 1.755 ± 0.377
3.663AspPro: 3.663 ± 0.733
2.9AspGln: 2.9 ± 0.476
4.502AspArg: 4.502 ± 0.562
2.289AspSer: 2.289 ± 0.549
2.9AspThr: 2.9 ± 0.499
4.121AspVal: 4.121 ± 0.623
1.145AspTrp: 1.145 ± 0.236
1.526AspTyr: 1.526 ± 0.329
0.0AspXaa: 0.0 ± 0.0
Glu
6.563GluAla: 6.563 ± 0.777
0.382GluCys: 0.382 ± 0.175
3.281GluAsp: 3.281 ± 0.55
3.739GluGlu: 3.739 ± 0.568
1.221GluPhe: 1.221 ± 0.255
4.197GluGly: 4.197 ± 0.572
0.992GluHis: 0.992 ± 0.215
2.442GluIle: 2.442 ± 0.38
1.908GluLys: 1.908 ± 0.437
5.037GluLeu: 5.037 ± 0.646
1.526GluMet: 1.526 ± 0.307
0.687GluAsn: 0.687 ± 0.203
2.671GluPro: 2.671 ± 0.498
3.129GluGln: 3.129 ± 0.346
3.968GluArg: 3.968 ± 0.563
3.053GluSer: 3.053 ± 0.651
2.824GluThr: 2.824 ± 0.372
3.739GluVal: 3.739 ± 0.508
1.908GluTrp: 1.908 ± 0.507
1.603GluTyr: 1.603 ± 0.525
0.0GluXaa: 0.0 ± 0.0
Phe
2.824PheAla: 2.824 ± 0.585
0.305PheCys: 0.305 ± 0.143
2.213PheAsp: 2.213 ± 0.316
0.992PheGlu: 0.992 ± 0.227
0.611PhePhe: 0.611 ± 0.274
1.908PheGly: 1.908 ± 0.354
0.839PheHis: 0.839 ± 0.286
0.763PheIle: 0.763 ± 0.197
1.755PheLys: 1.755 ± 0.382
1.755PheLeu: 1.755 ± 0.445
0.534PheMet: 0.534 ± 0.226
0.611PheAsn: 0.611 ± 0.228
1.221PhePro: 1.221 ± 0.322
0.763PheGln: 0.763 ± 0.185
1.526PheArg: 1.526 ± 0.357
1.068PheSer: 1.068 ± 0.28
1.984PheThr: 1.984 ± 0.391
1.679PheVal: 1.679 ± 0.339
0.382PheTrp: 0.382 ± 0.202
0.687PheTyr: 0.687 ± 0.2
0.0PheXaa: 0.0 ± 0.0
Gly
8.318GlyAla: 8.318 ± 1.251
0.763GlyCys: 0.763 ± 0.319
5.113GlyAsp: 5.113 ± 0.614
4.045GlyGlu: 4.045 ± 0.626
2.442GlyPhe: 2.442 ± 0.355
6.716GlyGly: 6.716 ± 1.119
1.908GlyHis: 1.908 ± 0.376
3.739GlyIle: 3.739 ± 0.714
3.968GlyLys: 3.968 ± 0.595
7.25GlyLeu: 7.25 ± 0.821
2.366GlyMet: 2.366 ± 0.432
3.129GlyAsn: 3.129 ± 0.532
4.197GlyPro: 4.197 ± 0.611
3.434GlyGln: 3.434 ± 0.427
5.952GlyArg: 5.952 ± 0.724
4.579GlySer: 4.579 ± 0.844
6.868GlyThr: 6.868 ± 1.111
5.266GlyVal: 5.266 ± 0.85
2.595GlyTrp: 2.595 ± 0.558
2.289GlyTyr: 2.289 ± 0.447
0.0GlyXaa: 0.0 ± 0.0
His
1.832HisAla: 1.832 ± 0.461
0.076HisCys: 0.076 ± 0.08
1.45HisAsp: 1.45 ± 0.343
1.526HisGlu: 1.526 ± 0.306
0.458HisPhe: 0.458 ± 0.2
1.526HisGly: 1.526 ± 0.436
0.687HisHis: 0.687 ± 0.318
0.153HisIle: 0.153 ± 0.161
1.068HisLys: 1.068 ± 0.296
1.755HisLeu: 1.755 ± 0.436
0.305HisMet: 0.305 ± 0.161
0.229HisAsn: 0.229 ± 0.137
1.068HisPro: 1.068 ± 0.335
1.068HisGln: 1.068 ± 0.325
1.374HisArg: 1.374 ± 0.403
1.221HisSer: 1.221 ± 0.34
1.374HisThr: 1.374 ± 0.391
1.832HisVal: 1.832 ± 0.369
0.458HisTrp: 0.458 ± 0.214
0.916HisTyr: 0.916 ± 0.246
0.0HisXaa: 0.0 ± 0.0
Ile
5.647IleAla: 5.647 ± 0.687
0.305IleCys: 0.305 ± 0.141
3.053IleAsp: 3.053 ± 0.52
3.129IleGlu: 3.129 ± 0.477
0.687IlePhe: 0.687 ± 0.185
2.824IleGly: 2.824 ± 0.471
0.839IleHis: 0.839 ± 0.28
1.45IleIle: 1.45 ± 0.361
1.679IleLys: 1.679 ± 0.368
2.06IleLeu: 2.06 ± 0.331
0.763IleMet: 0.763 ± 0.209
1.755IleAsn: 1.755 ± 0.381
2.9IlePro: 2.9 ± 0.414
1.145IleGln: 1.145 ± 0.263
3.434IleArg: 3.434 ± 0.52
2.518IleSer: 2.518 ± 0.497
4.197IleThr: 4.197 ± 0.512
2.671IleVal: 2.671 ± 0.607
0.458IleTrp: 0.458 ± 0.173
1.221IleTyr: 1.221 ± 0.294
0.0IleXaa: 0.0 ± 0.0
Lys
6.944LysAla: 6.944 ± 0.883
0.229LysCys: 0.229 ± 0.154
2.213LysAsp: 2.213 ± 0.416
2.518LysGlu: 2.518 ± 0.374
0.992LysPhe: 0.992 ± 0.268
3.281LysGly: 3.281 ± 0.541
1.221LysHis: 1.221 ± 0.363
1.297LysIle: 1.297 ± 0.316
1.755LysLys: 1.755 ± 0.369
3.816LysLeu: 3.816 ± 0.546
0.839LysMet: 0.839 ± 0.241
2.137LysAsn: 2.137 ± 0.373
3.129LysPro: 3.129 ± 0.503
2.06LysGln: 2.06 ± 0.441
2.595LysArg: 2.595 ± 0.524
2.213LysSer: 2.213 ± 0.561
3.053LysThr: 3.053 ± 0.52
3.51LysVal: 3.51 ± 0.566
0.611LysTrp: 0.611 ± 0.237
1.145LysTyr: 1.145 ± 0.305
0.0LysXaa: 0.0 ± 0.0
Leu
9.692LeuAla: 9.692 ± 0.891
0.611LeuCys: 0.611 ± 0.279
5.723LeuAsp: 5.723 ± 0.673
3.892LeuGlu: 3.892 ± 0.611
1.679LeuPhe: 1.679 ± 0.352
5.876LeuGly: 5.876 ± 0.691
1.526LeuHis: 1.526 ± 0.43
4.121LeuIle: 4.121 ± 0.581
4.045LeuLys: 4.045 ± 0.78
5.647LeuLeu: 5.647 ± 0.761
1.679LeuMet: 1.679 ± 0.33
2.289LeuAsn: 2.289 ± 0.404
4.35LeuPro: 4.35 ± 0.499
3.587LeuGln: 3.587 ± 0.603
5.037LeuArg: 5.037 ± 0.608
4.884LeuSer: 4.884 ± 0.763
5.723LeuThr: 5.723 ± 0.691
5.113LeuVal: 5.113 ± 0.754
1.068LeuTrp: 1.068 ± 0.323
2.06LeuTyr: 2.06 ± 0.345
0.0LeuXaa: 0.0 ± 0.0
Met
2.747MetAla: 2.747 ± 0.398
0.153MetCys: 0.153 ± 0.116
1.145MetAsp: 1.145 ± 0.306
0.611MetGlu: 0.611 ± 0.21
0.458MetPhe: 0.458 ± 0.223
1.679MetGly: 1.679 ± 0.369
0.229MetHis: 0.229 ± 0.126
0.687MetIle: 0.687 ± 0.217
0.611MetLys: 0.611 ± 0.181
1.374MetLeu: 1.374 ± 0.367
0.076MetMet: 0.076 ± 0.068
0.611MetAsn: 0.611 ± 0.23
1.297MetPro: 1.297 ± 0.334
0.534MetGln: 0.534 ± 0.169
1.374MetArg: 1.374 ± 0.305
2.289MetSer: 2.289 ± 0.394
2.9MetThr: 2.9 ± 0.503
1.145MetVal: 1.145 ± 0.237
0.229MetTrp: 0.229 ± 0.137
0.305MetTyr: 0.305 ± 0.158
0.0MetXaa: 0.0 ± 0.0
Asn
2.518AsnAla: 2.518 ± 0.435
0.153AsnCys: 0.153 ± 0.11
1.145AsnAsp: 1.145 ± 0.275
1.221AsnGlu: 1.221 ± 0.318
0.458AsnPhe: 0.458 ± 0.168
3.663AsnGly: 3.663 ± 0.573
0.763AsnHis: 0.763 ± 0.244
2.289AsnIle: 2.289 ± 0.443
0.992AsnLys: 0.992 ± 0.291
2.366AsnLeu: 2.366 ± 0.423
0.382AsnMet: 0.382 ± 0.172
0.763AsnAsn: 0.763 ± 0.284
3.434AsnPro: 3.434 ± 0.827
1.679AsnGln: 1.679 ± 0.378
1.45AsnArg: 1.45 ± 0.351
1.832AsnSer: 1.832 ± 0.462
2.442AsnThr: 2.442 ± 0.528
2.137AsnVal: 2.137 ± 0.412
0.229AsnTrp: 0.229 ± 0.146
0.839AsnTyr: 0.839 ± 0.229
0.0AsnXaa: 0.0 ± 0.0
Pro
7.631ProAla: 7.631 ± 1.384
0.534ProCys: 0.534 ± 0.251
3.129ProAsp: 3.129 ± 0.639
4.197ProGlu: 4.197 ± 0.702
1.832ProPhe: 1.832 ± 0.351
4.655ProGly: 4.655 ± 0.779
0.687ProHis: 0.687 ± 0.252
2.137ProIle: 2.137 ± 0.422
2.06ProLys: 2.06 ± 0.368
4.502ProLeu: 4.502 ± 0.66
1.45ProMet: 1.45 ± 0.316
1.908ProAsn: 1.908 ± 0.589
3.739ProPro: 3.739 ± 0.872
2.213ProGln: 2.213 ± 0.618
3.358ProArg: 3.358 ± 0.638
3.053ProSer: 3.053 ± 0.468
3.587ProThr: 3.587 ± 0.637
4.731ProVal: 4.731 ± 0.674
1.145ProTrp: 1.145 ± 0.355
1.679ProTyr: 1.679 ± 0.344
0.0ProXaa: 0.0 ± 0.0
Gln
5.342GlnAla: 5.342 ± 0.892
0.382GlnCys: 0.382 ± 0.186
2.747GlnAsp: 2.747 ± 0.51
1.755GlnGlu: 1.755 ± 0.31
1.068GlnPhe: 1.068 ± 0.303
2.9GlnGly: 2.9 ± 0.46
0.534GlnHis: 0.534 ± 0.189
1.832GlnIle: 1.832 ± 0.375
2.289GlnLys: 2.289 ± 0.368
3.892GlnLeu: 3.892 ± 0.511
1.221GlnMet: 1.221 ± 0.299
0.687GlnAsn: 0.687 ± 0.213
2.366GlnPro: 2.366 ± 0.533
1.984GlnGln: 1.984 ± 0.461
2.976GlnArg: 2.976 ± 0.482
2.595GlnSer: 2.595 ± 0.472
2.366GlnThr: 2.366 ± 0.497
2.595GlnVal: 2.595 ± 0.473
0.382GlnTrp: 0.382 ± 0.225
1.068GlnTyr: 1.068 ± 0.266
0.0GlnXaa: 0.0 ± 0.0
Arg
6.105ArgAla: 6.105 ± 0.729
1.374ArgCys: 1.374 ± 0.476
3.587ArgAsp: 3.587 ± 0.563
3.816ArgGlu: 3.816 ± 0.665
1.145ArgPhe: 1.145 ± 0.298
5.037ArgGly: 5.037 ± 0.719
1.221ArgHis: 1.221 ± 0.369
2.671ArgIle: 2.671 ± 0.484
3.663ArgLys: 3.663 ± 0.666
5.876ArgLeu: 5.876 ± 0.778
1.755ArgMet: 1.755 ± 0.347
1.984ArgAsn: 1.984 ± 0.415
3.281ArgPro: 3.281 ± 0.57
1.755ArgGln: 1.755 ± 0.326
5.113ArgArg: 5.113 ± 0.603
3.739ArgSer: 3.739 ± 0.482
4.655ArgThr: 4.655 ± 0.602
5.495ArgVal: 5.495 ± 0.803
2.06ArgTrp: 2.06 ± 0.509
1.374ArgTyr: 1.374 ± 0.36
0.0ArgXaa: 0.0 ± 0.0
Ser
6.487SerAla: 6.487 ± 0.833
0.763SerCys: 0.763 ± 0.257
3.434SerAsp: 3.434 ± 0.567
2.976SerGlu: 2.976 ± 0.593
1.221SerPhe: 1.221 ± 0.328
5.342SerGly: 5.342 ± 0.839
1.145SerHis: 1.145 ± 0.336
2.595SerIle: 2.595 ± 0.664
3.205SerLys: 3.205 ± 0.588
3.892SerLeu: 3.892 ± 0.6
1.068SerMet: 1.068 ± 0.326
1.297SerAsn: 1.297 ± 0.293
2.366SerPro: 2.366 ± 0.4
2.213SerGln: 2.213 ± 0.43
3.739SerArg: 3.739 ± 0.701
1.832SerSer: 1.832 ± 0.509
4.121SerThr: 4.121 ± 0.767
3.739SerVal: 3.739 ± 0.581
0.992SerTrp: 0.992 ± 0.263
1.679SerTyr: 1.679 ± 0.311
0.0SerXaa: 0.0 ± 0.0
Thr
8.929ThrAla: 8.929 ± 0.774
0.534ThrCys: 0.534 ± 0.21
3.51ThrAsp: 3.51 ± 0.464
3.663ThrGlu: 3.663 ± 0.674
2.289ThrPhe: 2.289 ± 0.484
6.792ThrGly: 6.792 ± 0.748
1.526ThrHis: 1.526 ± 0.331
3.587ThrIle: 3.587 ± 0.493
2.366ThrLys: 2.366 ± 0.527
5.876ThrLeu: 5.876 ± 0.678
0.839ThrMet: 0.839 ± 0.229
1.908ThrAsn: 1.908 ± 0.425
4.808ThrPro: 4.808 ± 0.903
2.442ThrGln: 2.442 ± 0.356
3.892ThrArg: 3.892 ± 0.495
3.816ThrSer: 3.816 ± 0.777
5.113ThrThr: 5.113 ± 0.802
6.105ThrVal: 6.105 ± 0.933
1.297ThrTrp: 1.297 ± 0.328
2.595ThrTyr: 2.595 ± 0.417
0.0ThrXaa: 0.0 ± 0.0
Val
8.089ValAla: 8.089 ± 0.81
0.458ValCys: 0.458 ± 0.177
3.358ValAsp: 3.358 ± 0.533
4.274ValGlu: 4.274 ± 0.587
1.221ValPhe: 1.221 ± 0.317
5.495ValGly: 5.495 ± 0.776
1.603ValHis: 1.603 ± 0.412
3.358ValIle: 3.358 ± 0.544
3.663ValLys: 3.663 ± 0.565
4.426ValLeu: 4.426 ± 0.536
1.068ValMet: 1.068 ± 0.275
2.671ValAsn: 2.671 ± 0.505
4.121ValPro: 4.121 ± 0.509
2.06ValGln: 2.06 ± 0.474
4.655ValArg: 4.655 ± 0.691
4.96ValSer: 4.96 ± 0.687
5.342ValThr: 5.342 ± 0.589
4.579ValVal: 4.579 ± 0.721
1.603ValTrp: 1.603 ± 0.377
1.526ValTyr: 1.526 ± 0.36
0.0ValXaa: 0.0 ± 0.0
Trp
2.289TrpAla: 2.289 ± 0.407
0.382TrpCys: 0.382 ± 0.159
1.755TrpAsp: 1.755 ± 0.423
1.374TrpGlu: 1.374 ± 0.348
0.687TrpPhe: 0.687 ± 0.204
0.992TrpGly: 0.992 ± 0.243
0.458TrpHis: 0.458 ± 0.22
0.916TrpIle: 0.916 ± 0.268
0.687TrpLys: 0.687 ± 0.242
1.832TrpLeu: 1.832 ± 0.528
0.382TrpMet: 0.382 ± 0.183
0.611TrpAsn: 0.611 ± 0.233
0.763TrpPro: 0.763 ± 0.301
1.145TrpGln: 1.145 ± 0.258
1.221TrpArg: 1.221 ± 0.358
0.992TrpSer: 0.992 ± 0.275
0.839TrpThr: 0.839 ± 0.179
1.603TrpVal: 1.603 ± 0.413
0.763TrpTrp: 0.763 ± 0.239
0.305TrpTyr: 0.305 ± 0.16
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.824TyrAla: 2.824 ± 0.564
0.382TyrCys: 0.382 ± 0.177
1.297TyrAsp: 1.297 ± 0.284
1.374TyrGlu: 1.374 ± 0.298
0.687TyrPhe: 0.687 ± 0.251
2.289TyrGly: 2.289 ± 0.403
0.153TyrHis: 0.153 ± 0.094
1.145TyrIle: 1.145 ± 0.319
0.839TyrLys: 0.839 ± 0.208
2.289TyrLeu: 2.289 ± 0.541
0.382TyrMet: 0.382 ± 0.156
1.221TyrAsn: 1.221 ± 0.316
1.297TyrPro: 1.297 ± 0.353
1.679TyrGln: 1.679 ± 0.422
2.137TyrArg: 2.137 ± 0.436
1.068TyrSer: 1.068 ± 0.191
2.289TyrThr: 2.289 ± 0.422
1.374TyrVal: 1.374 ± 0.333
0.458TyrTrp: 0.458 ± 0.174
0.611TyrTyr: 0.611 ± 0.249
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 63 proteins (13105 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski