Sewage-associated gemycircularvirus-10b

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemykibivirus; Gemykibivirus humas1; Human associated gemykibivirus 1

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A7CL82|A0A0A7CL82_9VIRU Replication-associated protein OS=Sewage-associated gemycircularvirus-10b OX=1519410 PE=3 SV=1
MM1 pKa = 7.83SSFRR5 pKa = 11.84FQARR9 pKa = 11.84YY10 pKa = 9.86ALLTYY15 pKa = 7.21PQCGDD20 pKa = 3.86LDD22 pKa = 3.85PFAVVNHH29 pKa = 6.38LAGLGAEE36 pKa = 4.46CIIGRR41 pKa = 11.84EE42 pKa = 3.88NHH44 pKa = 6.16TDD46 pKa = 3.34GGLHH50 pKa = 6.08LHH52 pKa = 6.6AFVDD56 pKa = 4.51FGVKK60 pKa = 9.88YY61 pKa = 8.61RR62 pKa = 11.84TRR64 pKa = 11.84NARR67 pKa = 11.84AFDD70 pKa = 3.89VEE72 pKa = 4.6GCHH75 pKa = 6.51PNVSPSRR82 pKa = 11.84GTPEE86 pKa = 3.58EE87 pKa = 4.81GYY89 pKa = 10.84DD90 pKa = 3.63YY91 pKa = 10.79AIKK94 pKa = 10.96DD95 pKa = 3.59GDD97 pKa = 4.01VVAGGLEE104 pKa = 4.09RR105 pKa = 11.84PSGSRR110 pKa = 11.84VDD112 pKa = 3.91ANGGVWAEE120 pKa = 4.11IINAKK125 pKa = 9.97SEE127 pKa = 4.02QEE129 pKa = 3.88FWDD132 pKa = 3.5LCEE135 pKa = 4.08RR136 pKa = 11.84LAPRR140 pKa = 11.84SLVTSFTQLRR150 pKa = 11.84AYY152 pKa = 9.55AAWKK156 pKa = 9.13FPPIRR161 pKa = 11.84VPYY164 pKa = 6.94EE165 pKa = 3.74TPEE168 pKa = 4.05GVNIDD173 pKa = 3.83TSWVVEE179 pKa = 4.03LDD181 pKa = 2.98QWVQQNLGRR190 pKa = 11.84SEE192 pKa = 4.28TGGKK196 pKa = 9.33

Molecular weight: 21.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A7CL99|A0A0A7CL99_9VIRU Capsid protein OS=Sewage-associated gemycircularvirus-10b OX=1519410 PE=4 SV=1
MM1 pKa = 7.3SLARR5 pKa = 11.84RR6 pKa = 11.84PVRR9 pKa = 11.84RR10 pKa = 11.84SRR12 pKa = 11.84KK13 pKa = 6.36TYY15 pKa = 9.4RR16 pKa = 11.84RR17 pKa = 11.84GGRR20 pKa = 11.84VTRR23 pKa = 11.84SRR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84STRR30 pKa = 11.84TSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84VGRR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84MTTRR45 pKa = 11.84RR46 pKa = 11.84VLNIASRR53 pKa = 11.84KK54 pKa = 9.62KK55 pKa = 9.56VDD57 pKa = 3.09NMLPVVVAEE66 pKa = 4.69DD67 pKa = 3.42NVTTVGPYY75 pKa = 9.07TSPSPLLCLFVPNARR90 pKa = 11.84TTRR93 pKa = 11.84TPVTNPAVRR102 pKa = 11.84NSSDD106 pKa = 2.87IFAVGYY112 pKa = 9.14KK113 pKa = 9.64EE114 pKa = 4.03RR115 pKa = 11.84VRR117 pKa = 11.84IDD119 pKa = 3.09ILGGGTFMWRR129 pKa = 11.84RR130 pKa = 11.84IVFMLKK136 pKa = 10.54GSDD139 pKa = 3.5LRR141 pKa = 11.84VAMDD145 pKa = 3.85SSNAGNIPNQLFDD158 pKa = 3.24QTTEE162 pKa = 4.13GGCRR166 pKa = 11.84RR167 pKa = 11.84VIGPLLGVTNAQDD180 pKa = 3.51EE181 pKa = 4.51LQSYY185 pKa = 7.05VFRR188 pKa = 11.84GQEE191 pKa = 3.75DD192 pKa = 4.44TDD194 pKa = 3.49WADD197 pKa = 3.34QFTAPLDD204 pKa = 3.58TRR206 pKa = 11.84RR207 pKa = 11.84ITVHH211 pKa = 5.82SDD213 pKa = 2.41KK214 pKa = 10.97LRR216 pKa = 11.84TIRR219 pKa = 11.84PGNDD223 pKa = 2.46TGASRR228 pKa = 11.84LYY230 pKa = 10.16KK231 pKa = 9.37CWYY234 pKa = 8.3PIRR237 pKa = 11.84RR238 pKa = 11.84TIAYY242 pKa = 9.2EE243 pKa = 4.32DD244 pKa = 4.14DD245 pKa = 4.25LEE247 pKa = 6.16SDD249 pKa = 4.19VVGDD253 pKa = 4.43RR254 pKa = 11.84PFSTAGLRR262 pKa = 11.84GVGDD266 pKa = 4.13MYY268 pKa = 11.73VMDD271 pKa = 4.6IMGITNLTPDD281 pKa = 4.03APEE284 pKa = 3.46TSYY287 pKa = 11.17RR288 pKa = 11.84FSPEE292 pKa = 3.23GSFYY296 pKa = 9.4WHH298 pKa = 6.64EE299 pKa = 4.04RR300 pKa = 3.36

Molecular weight: 34.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

807

196

311

269.0

30.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.691 ± 0.833

1.487 ± 0.237

6.815 ± 0.24

4.957 ± 0.853

4.461 ± 0.551

8.922 ± 0.59

1.859 ± 0.531

3.965 ± 0.175

3.222 ± 0.624

7.311 ± 0.533

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.487 ± 0.673

4.213 ± 0.141

5.452 ± 0.15

2.726 ± 0.357

9.789 ± 2.333

6.444 ± 0.311

5.948 ± 1.537

8.055 ± 0.167

2.354 ± 0.487

3.841 ± 0.324
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski