Caballeronia insecticola
Average proteome isoelectric point is 6.81
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6724 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R4WGJ1|R4WGJ1_9BURK LexA repressor OS=Caballeronia insecticola OX=758793 GN=lexA PE=3 SV=1
MM1 pKa = 7.66 SEE3 pKa = 4.05 VIEE6 pKa = 4.22 YY7 pKa = 10.35 KK8 pKa = 10.44 SWVCLICGWIYY19 pKa = 11.0 NEE21 pKa = 4.11 EE22 pKa = 4.61 DD23 pKa = 3.62 GLPEE27 pKa = 5.29 DD28 pKa = 5.57 GIAAGTRR35 pKa = 11.84 FGEE38 pKa = 4.66 IPDD41 pKa = 3.74 DD42 pKa = 3.9 WRR44 pKa = 11.84 CPLCDD49 pKa = 3.13 VGKK52 pKa = 10.52 ADD54 pKa = 3.73 FVVVEE59 pKa = 5.07 FF60 pKa = 4.82
Molecular weight: 6.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.779
IPC2_protein 3.923
IPC_protein 3.795
Toseland 3.617
ProMoST 3.961
Dawson 3.783
Bjellqvist 3.948
Wikipedia 3.706
Rodwell 3.643
Grimsley 3.541
Solomon 3.757
Lehninger 3.706
Nozaki 3.91
DTASelect 4.062
Thurlkill 3.681
EMBOSS 3.719
Sillero 3.923
Patrickios 0.693
IPC_peptide 3.757
IPC2_peptide 3.897
IPC2.peptide.svr19 3.822
Protein with the highest isoelectric point:
>tr|R4WPK3|R4WPK3_9BURK Uncharacterized protein OS=Caballeronia insecticola OX=758793 GN=BRPE64_ACDS10310 PE=4 SV=1
MM1 pKa = 7.67 KK2 pKa = 10.35 KK3 pKa = 10.29 NPFLSMWLSGANAVVGSTRR22 pKa = 11.84 GRR24 pKa = 11.84 ATAQAKK30 pKa = 9.51 RR31 pKa = 11.84 QVSNFWSAALAPSKK45 pKa = 9.92 PKK47 pKa = 10.11 KK48 pKa = 9.61 RR49 pKa = 11.84 RR50 pKa = 11.84 ARR52 pKa = 11.84 RR53 pKa = 3.28
Molecular weight: 5.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.475
IPC2_protein 11.052
IPC_protein 12.618
Toseland 12.793
ProMoST 13.29
Dawson 12.793
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.574
Grimsley 12.837
Solomon 13.29
Lehninger 13.188
Nozaki 12.793
DTASelect 12.778
Thurlkill 12.793
EMBOSS 13.29
Sillero 12.793
Patrickios 12.31
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6724
0
6724
2040670
37
3414
303.5
32.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.856 ± 0.048
0.947 ± 0.009
5.551 ± 0.024
5.163 ± 0.029
3.822 ± 0.019
8.052 ± 0.03
2.346 ± 0.016
4.918 ± 0.021
3.231 ± 0.028
10.01 ± 0.035
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.512 ± 0.016
2.811 ± 0.018
4.983 ± 0.022
3.411 ± 0.018
7.032 ± 0.033
5.673 ± 0.024
5.339 ± 0.023
7.615 ± 0.024
1.34 ± 0.012
2.386 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here