Dialister invisus CAG:218

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Negativicutes; Veillonellales; Veillonellaceae; Dialister; environmental samples

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1693 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5SJB9|R5SJB9_9FIRM Uncharacterized protein OS=Dialister invisus CAG:218 OX=1263072 GN=BN540_00303 PE=4 SV=1
MM1 pKa = 7.61EE2 pKa = 5.56FLNEE6 pKa = 3.64KK7 pKa = 10.65NMIVDD12 pKa = 4.33GVDD15 pKa = 2.74KK16 pKa = 11.2YY17 pKa = 11.44YY18 pKa = 10.98VSEE21 pKa = 4.37LKK23 pKa = 10.21RR24 pKa = 11.84VYY26 pKa = 10.77GSAEE30 pKa = 3.7YY31 pKa = 10.54DD32 pKa = 3.26GEE34 pKa = 4.37EE35 pKa = 4.46FVFLYY40 pKa = 10.66DD41 pKa = 4.61PEE43 pKa = 4.74QEE45 pKa = 4.35DD46 pKa = 3.55TTRR49 pKa = 11.84AYY51 pKa = 10.66RR52 pKa = 11.84PDD54 pKa = 3.84SFVMAVMMDD63 pKa = 3.67DD64 pKa = 4.44EE65 pKa = 5.15IFDD68 pKa = 4.16GKK70 pKa = 10.13VLQYY74 pKa = 11.21LVTLSYY80 pKa = 10.78DD81 pKa = 3.43GHH83 pKa = 6.7VKK85 pKa = 10.04GVEE88 pKa = 3.73YY89 pKa = 10.85LGLDD93 pKa = 3.38DD94 pKa = 5.84AGRR97 pKa = 11.84PDD99 pKa = 3.76AVDD102 pKa = 3.46VEE104 pKa = 4.61

Molecular weight: 11.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5ST28|R5ST28_9FIRM Hydrogenase expression/formation protein HypE OS=Dialister invisus CAG:218 OX=1263072 GN=BN540_00327 PE=3 SV=1
MM1 pKa = 7.7AKK3 pKa = 7.99MTFQPNNHH11 pKa = 5.37WRR13 pKa = 11.84KK14 pKa = 7.39HH15 pKa = 3.96THH17 pKa = 5.35GFRR20 pKa = 11.84ARR22 pKa = 11.84MKK24 pKa = 8.64TKK26 pKa = 10.33AGRR29 pKa = 11.84IVLKK33 pKa = 10.36RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 9.89GRR40 pKa = 11.84KK41 pKa = 8.67VLSAA45 pKa = 4.05

Molecular weight: 5.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1693

0

1693

497010

30

1530

293.6

32.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.439 ± 0.065

1.249 ± 0.026

5.399 ± 0.044

6.977 ± 0.074

4.296 ± 0.051

7.777 ± 0.061

2.076 ± 0.027

7.28 ± 0.051

6.624 ± 0.053

9.02 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.197 ± 0.031

3.767 ± 0.054

3.65 ± 0.037

2.683 ± 0.033

5.106 ± 0.052

5.708 ± 0.045

5.103 ± 0.042

6.983 ± 0.053

1.041 ± 0.023

3.626 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski