Tursiops truncatus papillomavirus 2
Average proteome isoelectric point is 6.5
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q29UC8|Q29UC8_9PAPI Major capsid protein L1 OS=Tursiops truncatus papillomavirus 2 OX=936060 GN=L1 PE=3 SV=1
MM1 pKa = 7.94 GDD3 pKa = 3.54 RR4 pKa = 11.84 EE5 pKa = 4.28 NACMEE10 pKa = 3.93 RR11 pKa = 11.84 MEE13 pKa = 4.32 EE14 pKa = 3.91 VDD16 pKa = 4.42 RR17 pKa = 11.84 GDD19 pKa = 3.43 RR20 pKa = 11.84 DD21 pKa = 3.65 EE22 pKa = 6.04 GPVEE26 pKa = 4.07 YY27 pKa = 10.76 LNEE30 pKa = 4.02 LCAQLHH36 pKa = 5.98 LPVEE40 pKa = 4.72 EE41 pKa = 4.83 ILINCCFCNCQLSEE55 pKa = 4.01 RR56 pKa = 11.84 SKK58 pKa = 10.45 WEE60 pKa = 3.76 QIYY63 pKa = 11.06 KK64 pKa = 10.32 DD65 pKa = 4.29 FKK67 pKa = 10.74 LVWRR71 pKa = 11.84 EE72 pKa = 3.73 GWPHH76 pKa = 6.92 GICHH80 pKa = 6.82 KK81 pKa = 9.87 CQEE84 pKa = 3.9 IHH86 pKa = 6.4 AVKK89 pKa = 10.5 ASWRR93 pKa = 11.84 GYY95 pKa = 7.25 EE96 pKa = 3.81 QSAYY100 pKa = 9.39 ACYY103 pKa = 10.48 VEE105 pKa = 5.51 EE106 pKa = 4.29 EE107 pKa = 4.41 TGTPLGDD114 pKa = 2.69 IYY116 pKa = 10.59 MRR118 pKa = 11.84 CLYY121 pKa = 10.32 CWIPLTGAEE130 pKa = 4.63 KK131 pKa = 9.98 IHH133 pKa = 5.53 MVEE136 pKa = 4.84 DD137 pKa = 3.34 RR138 pKa = 11.84 RR139 pKa = 11.84 HH140 pKa = 5.62 FIKK143 pKa = 10.72 INGYY147 pKa = 8.79 WKK149 pKa = 10.31 GLCHH153 pKa = 6.59 RR154 pKa = 11.84 CLWNPPRR161 pKa = 11.84 LAAQLGGINVVLPHH175 pKa = 5.95 QEE177 pKa = 3.92 NEE179 pKa = 4.13 GDD181 pKa = 3.98 SEE183 pKa = 5.57 ADD185 pKa = 3.36 TEE187 pKa = 5.06 SIGTEE192 pKa = 4.07 DD193 pKa = 3.9 LSISAGEE200 pKa = 4.04 DD201 pKa = 3.38 LEE203 pKa = 4.64 TEE205 pKa = 4.34 LL206 pKa = 5.59
Molecular weight: 23.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.516
IPC2_protein 4.762
IPC_protein 4.685
Toseland 4.584
ProMoST 4.787
Dawson 4.635
Bjellqvist 4.774
Wikipedia 4.469
Rodwell 4.558
Grimsley 4.495
Solomon 4.635
Lehninger 4.584
Nozaki 4.749
DTASelect 4.838
Thurlkill 4.571
EMBOSS 4.495
Sillero 4.825
Patrickios 2.041
IPC_peptide 4.647
IPC2_peptide 4.813
IPC2.peptide.svr19 4.766
Protein with the highest isoelectric point:
>tr|Q1XA73|Q1XA73_9PAPI Regulatory protein E2 OS=Tursiops truncatus papillomavirus 2 OX=936060 GN=E2 PE=3 SV=1
MM1 pKa = 7.69 GLQKK5 pKa = 10.61 CVTLSGKK12 pKa = 10.21 EE13 pKa = 3.76 KK14 pKa = 10.17 VLQILVVCLEE24 pKa = 3.99 PTYY27 pKa = 10.75 PLLDD31 pKa = 4.01 LFRR34 pKa = 11.84 TTHH37 pKa = 5.18 LTKK40 pKa = 9.37 PTRR43 pKa = 11.84 SNKK46 pKa = 7.63 TSRR49 pKa = 11.84 TGAVPFPHH57 pKa = 6.51 QPANKK62 pKa = 9.62 PGAQQSKK69 pKa = 7.67 TASTSQATSTEE80 pKa = 4.38 HH81 pKa = 5.44 ATSTEE86 pKa = 3.96 GMDD89 pKa = 3.39 VEE91 pKa = 4.73 VTVTVQVDD99 pKa = 3.51 VGNKK103 pKa = 9.85 APIVLKK109 pKa = 10.49 FQLSS113 pKa = 3.44
Molecular weight: 12.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.613
IPC2_protein 8.492
IPC_protein 8.507
Toseland 9.414
ProMoST 8.99
Dawson 9.56
Bjellqvist 9.297
Wikipedia 9.619
Rodwell 10.131
Grimsley 9.531
Solomon 9.706
Lehninger 9.706
Nozaki 9.633
DTASelect 9.209
Thurlkill 9.472
EMBOSS 9.794
Sillero 9.575
Patrickios 10.116
IPC_peptide 9.706
IPC2_peptide 7.966
IPC2.peptide.svr19 7.589
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2482
103
624
354.6
39.3
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.89 ± 0.524
2.538 ± 0.695
5.479 ± 0.661
5.641 ± 1.025
3.707 ± 0.477
7.172 ± 0.536
3.022 ± 0.28
4.512 ± 0.476
5.036 ± 0.856
9.065 ± 1.529
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.135 ± 0.343
3.908 ± 0.659
6.164 ± 1.062
3.183 ± 0.328
5.157 ± 0.483
8.179 ± 0.953
6.769 ± 0.841
6.567 ± 0.558
1.612 ± 0.373
3.263 ± 0.475
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here