Amino acid dipepetide frequency for Mycobacterium phage Kimberlium

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.498AlaAla: 14.498 ± 1.848
1.204AlaCys: 1.204 ± 0.243
7.167AlaAsp: 7.167 ± 0.688
7.277AlaGlu: 7.277 ± 0.665
2.845AlaPhe: 2.845 ± 0.416
9.301AlaGly: 9.301 ± 1.156
2.626AlaHis: 2.626 ± 0.48
4.486AlaIle: 4.486 ± 0.544
4.815AlaLys: 4.815 ± 0.584
7.878AlaLeu: 7.878 ± 0.758
2.79AlaMet: 2.79 ± 0.436
2.954AlaAsn: 2.954 ± 0.449
5.471AlaPro: 5.471 ± 0.574
3.775AlaGln: 3.775 ± 0.461
7.112AlaArg: 7.112 ± 0.621
5.854AlaSer: 5.854 ± 0.713
7.277AlaThr: 7.277 ± 0.607
6.565AlaVal: 6.565 ± 0.609
2.407AlaTrp: 2.407 ± 0.41
2.353AlaTyr: 2.353 ± 0.334
0.0AlaXaa: 0.0 ± 0.0
Cys
0.985CysAla: 0.985 ± 0.329
0.109CysCys: 0.109 ± 0.077
1.149CysAsp: 1.149 ± 0.308
0.711CysGlu: 0.711 ± 0.215
0.109CysPhe: 0.109 ± 0.067
1.915CysGly: 1.915 ± 0.407
0.274CysHis: 0.274 ± 0.119
0.328CysIle: 0.328 ± 0.11
0.328CysLys: 0.328 ± 0.127
0.985CysLeu: 0.985 ± 0.237
0.164CysMet: 0.164 ± 0.128
0.492CysAsn: 0.492 ± 0.165
1.313CysPro: 1.313 ± 0.255
0.328CysGln: 0.328 ± 0.169
0.711CysArg: 0.711 ± 0.238
0.93CysSer: 0.93 ± 0.27
0.711CysThr: 0.711 ± 0.243
0.766CysVal: 0.766 ± 0.21
0.219CysTrp: 0.219 ± 0.102
0.164CysTyr: 0.164 ± 0.09
0.0CysXaa: 0.0 ± 0.0
Asp
6.784AspAla: 6.784 ± 0.638
0.93AspCys: 0.93 ± 0.163
4.322AspAsp: 4.322 ± 0.527
3.775AspGlu: 3.775 ± 0.644
1.587AspPhe: 1.587 ± 0.264
6.292AspGly: 6.292 ± 0.463
1.422AspHis: 1.422 ± 0.303
2.626AspIle: 2.626 ± 0.396
1.641AspLys: 1.641 ± 0.31
5.963AspLeu: 5.963 ± 0.595
1.094AspMet: 1.094 ± 0.254
1.696AspAsn: 1.696 ± 0.357
5.362AspPro: 5.362 ± 0.637
2.407AspGln: 2.407 ± 0.309
5.033AspArg: 5.033 ± 0.621
3.064AspSer: 3.064 ± 0.456
3.447AspThr: 3.447 ± 0.462
4.377AspVal: 4.377 ± 0.658
1.641AspTrp: 1.641 ± 0.321
1.915AspTyr: 1.915 ± 0.333
0.0AspXaa: 0.0 ± 0.0
Glu
6.729GluAla: 6.729 ± 0.659
0.93GluCys: 0.93 ± 0.258
2.9GluAsp: 2.9 ± 0.348
3.119GluGlu: 3.119 ± 0.478
2.024GluPhe: 2.024 ± 0.289
3.119GluGly: 3.119 ± 0.37
1.587GluHis: 1.587 ± 0.362
2.462GluIle: 2.462 ± 0.37
2.188GluLys: 2.188 ± 0.357
4.979GluLeu: 4.979 ± 0.601
1.641GluMet: 1.641 ± 0.282
1.805GluAsn: 1.805 ± 0.294
3.064GluPro: 3.064 ± 0.383
2.571GluGln: 2.571 ± 0.455
4.869GluArg: 4.869 ± 0.535
2.954GluSer: 2.954 ± 0.342
3.501GluThr: 3.501 ± 0.472
4.103GluVal: 4.103 ± 0.515
1.313GluTrp: 1.313 ± 0.272
1.587GluTyr: 1.587 ± 0.335
0.0GluXaa: 0.0 ± 0.0
Phe
2.79PheAla: 2.79 ± 0.407
0.547PheCys: 0.547 ± 0.163
2.298PheAsp: 2.298 ± 0.346
1.587PheGlu: 1.587 ± 0.299
0.985PhePhe: 0.985 ± 0.255
2.9PheGly: 2.9 ± 0.664
0.492PheHis: 0.492 ± 0.139
1.258PheIle: 1.258 ± 0.335
1.368PheLys: 1.368 ± 0.292
1.915PheLeu: 1.915 ± 0.244
0.602PheMet: 0.602 ± 0.185
1.149PheAsn: 1.149 ± 0.355
1.751PhePro: 1.751 ± 0.32
0.875PheGln: 0.875 ± 0.256
1.587PheArg: 1.587 ± 0.303
1.587PheSer: 1.587 ± 0.264
2.243PheThr: 2.243 ± 0.376
1.751PheVal: 1.751 ± 0.244
0.438PheTrp: 0.438 ± 0.152
0.766PheTyr: 0.766 ± 0.242
0.0PheXaa: 0.0 ± 0.0
Gly
9.848GlyAla: 9.848 ± 1.097
1.094GlyCys: 1.094 ± 0.254
6.237GlyAsp: 6.237 ± 0.601
4.596GlyGlu: 4.596 ± 0.561
2.571GlyPhe: 2.571 ± 0.559
10.45GlyGly: 10.45 ± 2.15
1.696GlyHis: 1.696 ± 0.306
4.213GlyIle: 4.213 ± 0.523
2.954GlyLys: 2.954 ± 0.384
5.362GlyLeu: 5.362 ± 0.595
2.353GlyMet: 2.353 ± 0.462
2.845GlyAsn: 2.845 ± 0.401
4.049GlyPro: 4.049 ± 0.514
2.188GlyGln: 2.188 ± 0.477
5.69GlyArg: 5.69 ± 0.659
5.416GlySer: 5.416 ± 0.759
6.346GlyThr: 6.346 ± 0.713
6.292GlyVal: 6.292 ± 0.713
2.9GlyTrp: 2.9 ± 0.419
2.243GlyTyr: 2.243 ± 0.416
0.0GlyXaa: 0.0 ± 0.0
His
2.079HisAla: 2.079 ± 0.337
0.274HisCys: 0.274 ± 0.145
1.094HisAsp: 1.094 ± 0.248
1.204HisGlu: 1.204 ± 0.269
0.602HisPhe: 0.602 ± 0.156
1.97HisGly: 1.97 ± 0.314
0.875HisHis: 0.875 ± 0.284
1.258HisIle: 1.258 ± 0.346
0.821HisLys: 0.821 ± 0.186
1.86HisLeu: 1.86 ± 0.359
0.328HisMet: 0.328 ± 0.106
0.985HisAsn: 0.985 ± 0.257
1.532HisPro: 1.532 ± 0.345
0.711HisGln: 0.711 ± 0.21
1.86HisArg: 1.86 ± 0.339
0.985HisSer: 0.985 ± 0.23
1.641HisThr: 1.641 ± 0.357
1.313HisVal: 1.313 ± 0.299
0.657HisTrp: 0.657 ± 0.185
0.985HisTyr: 0.985 ± 0.21
0.0HisXaa: 0.0 ± 0.0
Ile
5.471IleAla: 5.471 ± 0.519
0.711IleCys: 0.711 ± 0.24
3.556IleAsp: 3.556 ± 0.403
3.173IleGlu: 3.173 ± 0.34
0.766IlePhe: 0.766 ± 0.247
4.267IleGly: 4.267 ± 0.524
1.477IleHis: 1.477 ± 0.309
1.313IleIle: 1.313 ± 0.245
1.422IleLys: 1.422 ± 0.244
2.626IleLeu: 2.626 ± 0.432
0.274IleMet: 0.274 ± 0.11
1.751IleAsn: 1.751 ± 0.288
2.517IlePro: 2.517 ± 0.326
1.477IleGln: 1.477 ± 0.278
2.407IleArg: 2.407 ± 0.385
2.298IleSer: 2.298 ± 0.396
3.556IleThr: 3.556 ± 0.388
3.064IleVal: 3.064 ± 0.396
0.875IleTrp: 0.875 ± 0.21
0.711IleTyr: 0.711 ± 0.219
0.0IleXaa: 0.0 ± 0.0
Lys
4.322LysAla: 4.322 ± 0.595
0.328LysCys: 0.328 ± 0.13
2.079LysAsp: 2.079 ± 0.296
1.368LysGlu: 1.368 ± 0.282
1.149LysPhe: 1.149 ± 0.226
2.462LysGly: 2.462 ± 0.325
0.985LysHis: 0.985 ± 0.219
0.985LysIle: 0.985 ± 0.263
1.97LysLys: 1.97 ± 0.329
2.9LysLeu: 2.9 ± 0.529
0.492LysMet: 0.492 ± 0.163
1.094LysAsn: 1.094 ± 0.27
3.009LysPro: 3.009 ± 0.366
1.422LysGln: 1.422 ± 0.231
2.517LysArg: 2.517 ± 0.43
2.298LysSer: 2.298 ± 0.357
2.517LysThr: 2.517 ± 0.372
2.407LysVal: 2.407 ± 0.37
0.711LysTrp: 0.711 ± 0.219
0.657LysTyr: 0.657 ± 0.165
0.0LysXaa: 0.0 ± 0.0
Leu
8.042LeuAla: 8.042 ± 0.875
0.657LeuCys: 0.657 ± 0.196
4.76LeuAsp: 4.76 ± 0.555
3.666LeuGlu: 3.666 ± 0.418
2.024LeuPhe: 2.024 ± 0.291
5.635LeuGly: 5.635 ± 0.466
0.875LeuHis: 0.875 ± 0.208
3.337LeuIle: 3.337 ± 0.43
2.626LeuLys: 2.626 ± 0.473
4.815LeuLeu: 4.815 ± 0.551
1.641LeuMet: 1.641 ± 0.321
2.353LeuAsn: 2.353 ± 0.356
4.979LeuPro: 4.979 ± 0.664
2.79LeuGln: 2.79 ± 0.453
5.69LeuArg: 5.69 ± 0.573
4.705LeuSer: 4.705 ± 0.529
5.526LeuThr: 5.526 ± 0.558
5.252LeuVal: 5.252 ± 0.56
1.04LeuTrp: 1.04 ± 0.262
2.079LeuTyr: 2.079 ± 0.353
0.0LeuXaa: 0.0 ± 0.0
Met
2.134MetAla: 2.134 ± 0.408
0.219MetCys: 0.219 ± 0.198
1.204MetAsp: 1.204 ± 0.252
0.821MetGlu: 0.821 ± 0.196
0.657MetPhe: 0.657 ± 0.172
1.97MetGly: 1.97 ± 0.366
0.219MetHis: 0.219 ± 0.11
0.657MetIle: 0.657 ± 0.207
0.821MetLys: 0.821 ± 0.208
1.641MetLeu: 1.641 ± 0.289
0.547MetMet: 0.547 ± 0.237
1.149MetAsn: 1.149 ± 0.214
1.477MetPro: 1.477 ± 0.26
0.383MetGln: 0.383 ± 0.149
1.422MetArg: 1.422 ± 0.243
2.954MetSer: 2.954 ± 0.507
1.86MetThr: 1.86 ± 0.286
1.422MetVal: 1.422 ± 0.299
0.383MetTrp: 0.383 ± 0.159
0.219MetTyr: 0.219 ± 0.105
0.0MetXaa: 0.0 ± 0.0
Asn
3.228AsnAla: 3.228 ± 0.344
0.219AsnCys: 0.219 ± 0.117
1.751AsnAsp: 1.751 ± 0.275
1.696AsnGlu: 1.696 ± 0.341
0.766AsnPhe: 0.766 ± 0.253
4.322AsnGly: 4.322 ± 0.591
0.93AsnHis: 0.93 ± 0.208
1.532AsnIle: 1.532 ± 0.4
0.875AsnLys: 0.875 ± 0.223
2.407AsnLeu: 2.407 ± 0.354
0.875AsnMet: 0.875 ± 0.225
1.696AsnAsn: 1.696 ± 0.363
2.681AsnPro: 2.681 ± 0.43
1.204AsnGln: 1.204 ± 0.321
2.188AsnArg: 2.188 ± 0.369
1.751AsnSer: 1.751 ± 0.294
2.079AsnThr: 2.079 ± 0.313
1.86AsnVal: 1.86 ± 0.333
0.492AsnTrp: 0.492 ± 0.121
0.766AsnTyr: 0.766 ± 0.175
0.0AsnXaa: 0.0 ± 0.0
Pro
6.018ProAla: 6.018 ± 0.487
0.875ProCys: 0.875 ± 0.232
4.596ProAsp: 4.596 ± 0.482
4.596ProGlu: 4.596 ± 0.475
2.079ProPhe: 2.079 ± 0.343
6.839ProGly: 6.839 ± 0.666
1.477ProHis: 1.477 ± 0.262
2.079ProIle: 2.079 ± 0.258
2.024ProLys: 2.024 ± 0.411
4.213ProLeu: 4.213 ± 0.546
0.985ProMet: 0.985 ± 0.307
1.97ProAsn: 1.97 ± 0.386
4.322ProPro: 4.322 ± 0.634
2.681ProGln: 2.681 ± 0.346
3.283ProArg: 3.283 ± 0.496
3.173ProSer: 3.173 ± 0.457
3.501ProThr: 3.501 ± 0.479
4.541ProVal: 4.541 ± 0.558
0.93ProTrp: 0.93 ± 0.214
2.024ProTyr: 2.024 ± 0.33
0.0ProXaa: 0.0 ± 0.0
Gln
5.033GlnAla: 5.033 ± 0.58
0.274GlnCys: 0.274 ± 0.145
1.368GlnAsp: 1.368 ± 0.266
1.641GlnGlu: 1.641 ± 0.341
1.094GlnPhe: 1.094 ± 0.244
2.407GlnGly: 2.407 ± 0.412
1.094GlnHis: 1.094 ± 0.255
1.696GlnIle: 1.696 ± 0.352
1.368GlnLys: 1.368 ± 0.247
2.954GlnLeu: 2.954 ± 0.427
0.602GlnMet: 0.602 ± 0.175
1.094GlnAsn: 1.094 ± 0.226
2.298GlnPro: 2.298 ± 0.406
1.641GlnGln: 1.641 ± 0.457
2.407GlnArg: 2.407 ± 0.355
2.736GlnSer: 2.736 ± 0.356
1.422GlnThr: 1.422 ± 0.358
2.134GlnVal: 2.134 ± 0.385
0.657GlnTrp: 0.657 ± 0.161
1.04GlnTyr: 1.04 ± 0.284
0.0GlnXaa: 0.0 ± 0.0
Arg
6.565ArgAla: 6.565 ± 0.683
1.258ArgCys: 1.258 ± 0.316
4.815ArgAsp: 4.815 ± 0.671
4.486ArgGlu: 4.486 ± 0.604
2.298ArgPhe: 2.298 ± 0.319
4.596ArgGly: 4.596 ± 0.459
1.641ArgHis: 1.641 ± 0.432
3.994ArgIle: 3.994 ± 0.539
2.243ArgLys: 2.243 ± 0.356
4.158ArgLeu: 4.158 ± 0.483
2.845ArgMet: 2.845 ± 0.396
2.243ArgAsn: 2.243 ± 0.447
3.611ArgPro: 3.611 ± 0.426
2.188ArgGln: 2.188 ± 0.389
5.198ArgArg: 5.198 ± 0.699
3.83ArgSer: 3.83 ± 0.447
3.775ArgThr: 3.775 ± 0.525
4.869ArgVal: 4.869 ± 0.458
1.641ArgTrp: 1.641 ± 0.266
2.024ArgTyr: 2.024 ± 0.358
0.0ArgXaa: 0.0 ± 0.0
Ser
5.362SerAla: 5.362 ± 0.714
0.492SerCys: 0.492 ± 0.183
4.103SerAsp: 4.103 ± 0.495
3.611SerGlu: 3.611 ± 0.395
2.188SerPhe: 2.188 ± 0.531
6.128SerGly: 6.128 ± 0.886
1.149SerHis: 1.149 ± 0.251
2.571SerIle: 2.571 ± 0.431
2.243SerLys: 2.243 ± 0.353
3.884SerLeu: 3.884 ± 0.509
1.587SerMet: 1.587 ± 0.251
2.079SerAsn: 2.079 ± 0.504
3.447SerPro: 3.447 ± 0.467
1.915SerGln: 1.915 ± 0.306
3.666SerArg: 3.666 ± 0.404
3.666SerSer: 3.666 ± 0.576
3.173SerThr: 3.173 ± 0.436
4.322SerVal: 4.322 ± 0.525
1.532SerTrp: 1.532 ± 0.298
1.422SerTyr: 1.422 ± 0.297
0.0SerXaa: 0.0 ± 0.0
Thr
6.511ThrAla: 6.511 ± 0.706
0.711ThrCys: 0.711 ± 0.264
3.556ThrAsp: 3.556 ± 0.601
3.611ThrGlu: 3.611 ± 0.432
1.696ThrPhe: 1.696 ± 0.308
5.799ThrGly: 5.799 ± 0.586
1.477ThrHis: 1.477 ± 0.318
3.775ThrIle: 3.775 ± 0.5
2.079ThrLys: 2.079 ± 0.341
4.76ThrLeu: 4.76 ± 0.555
0.985ThrMet: 0.985 ± 0.203
2.353ThrAsn: 2.353 ± 0.314
4.76ThrPro: 4.76 ± 0.737
1.86ThrGln: 1.86 ± 0.294
4.158ThrArg: 4.158 ± 0.475
3.666ThrSer: 3.666 ± 0.437
4.322ThrThr: 4.322 ± 0.492
5.307ThrVal: 5.307 ± 0.587
1.258ThrTrp: 1.258 ± 0.281
2.079ThrTyr: 2.079 ± 0.282
0.0ThrXaa: 0.0 ± 0.0
Val
7.824ValAla: 7.824 ± 0.604
1.258ValCys: 1.258 ± 0.287
5.307ValAsp: 5.307 ± 0.643
3.775ValGlu: 3.775 ± 0.567
1.915ValPhe: 1.915 ± 0.359
5.58ValGly: 5.58 ± 0.671
1.751ValHis: 1.751 ± 0.319
2.845ValIle: 2.845 ± 0.401
2.571ValLys: 2.571 ± 0.413
5.307ValLeu: 5.307 ± 0.595
1.204ValMet: 1.204 ± 0.247
2.298ValAsn: 2.298 ± 0.306
3.884ValPro: 3.884 ± 0.383
2.681ValGln: 2.681 ± 0.358
4.377ValArg: 4.377 ± 0.516
4.322ValSer: 4.322 ± 0.513
4.377ValThr: 4.377 ± 0.452
5.799ValVal: 5.799 ± 0.665
1.915ValTrp: 1.915 ± 0.374
1.313ValTyr: 1.313 ± 0.283
0.0ValXaa: 0.0 ± 0.0
Trp
1.86TrpAla: 1.86 ± 0.267
0.219TrpCys: 0.219 ± 0.104
1.368TrpAsp: 1.368 ± 0.276
1.149TrpGlu: 1.149 ± 0.276
0.602TrpPhe: 0.602 ± 0.152
1.204TrpGly: 1.204 ± 0.264
0.602TrpHis: 0.602 ± 0.178
1.258TrpIle: 1.258 ± 0.241
0.657TrpLys: 0.657 ± 0.157
1.696TrpLeu: 1.696 ± 0.315
0.93TrpMet: 0.93 ± 0.252
0.657TrpAsn: 0.657 ± 0.212
1.368TrpPro: 1.368 ± 0.298
1.094TrpGln: 1.094 ± 0.237
1.86TrpArg: 1.86 ± 0.303
1.258TrpSer: 1.258 ± 0.247
1.587TrpThr: 1.587 ± 0.325
1.696TrpVal: 1.696 ± 0.395
0.985TrpTrp: 0.985 ± 0.238
0.602TrpTyr: 0.602 ± 0.166
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.571TyrAla: 2.571 ± 0.413
0.383TyrCys: 0.383 ± 0.238
1.751TyrAsp: 1.751 ± 0.354
1.587TyrGlu: 1.587 ± 0.337
0.93TyrPhe: 0.93 ± 0.194
2.243TyrGly: 2.243 ± 0.48
0.383TyrHis: 0.383 ± 0.126
1.04TyrIle: 1.04 ± 0.276
0.657TyrLys: 0.657 ± 0.217
2.188TyrLeu: 2.188 ± 0.329
0.164TyrMet: 0.164 ± 0.105
0.657TyrAsn: 0.657 ± 0.153
1.313TyrPro: 1.313 ± 0.273
0.766TyrGln: 0.766 ± 0.193
2.243TyrArg: 2.243 ± 0.395
1.094TyrSer: 1.094 ± 0.242
1.86TyrThr: 1.86 ± 0.374
2.462TyrVal: 2.462 ± 0.304
0.657TyrTrp: 0.657 ± 0.199
0.602TyrTyr: 0.602 ± 0.173
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 105 proteins (18279 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski