Actinidia virus X
Average proteome isoelectric point is 6.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0S2ZXH8|A0A0S2ZXH8_9VIRU Triple gene block protein 2 OS=Actinidia virus X OX=1331744 PE=4 SV=1
MM1 pKa = 7.22 EE2 pKa = 5.77 LEE4 pKa = 4.09 YY5 pKa = 10.75 LINLLDD11 pKa = 3.91 FYY13 pKa = 11.47 GFVRR17 pKa = 11.84 SSRR20 pKa = 11.84 SFSLPLVIHH29 pKa = 6.41 GVAGCGKK36 pKa = 8.46 STIISKK42 pKa = 9.36 VSCAYY47 pKa = 9.17 PKK49 pKa = 10.48 LISASFSPQLIDD61 pKa = 3.61 PDD63 pKa = 3.61 SGRR66 pKa = 11.84 RR67 pKa = 11.84 QSVVDD72 pKa = 3.74 GTTVDD77 pKa = 5.21 LLDD80 pKa = 4.78 EE81 pKa = 4.27 YY82 pKa = 11.51 LAGNNPIVRR91 pKa = 11.84 LAKK94 pKa = 10.58 FSDD97 pKa = 3.57 PLQYY101 pKa = 10.9 DD102 pKa = 3.8 CQSPEE107 pKa = 3.97 EE108 pKa = 3.82 PHH110 pKa = 6.19 YY111 pKa = 10.65 RR112 pKa = 11.84 ALHH115 pKa = 4.38 SHH117 pKa = 6.82 RR118 pKa = 11.84 FCPATAQLLNKK129 pKa = 9.92 IFGCHH134 pKa = 4.92 ITSKK138 pKa = 10.65 LNTSAVIRR146 pKa = 11.84 FADD149 pKa = 3.96 AFTEE153 pKa = 4.28 DD154 pKa = 4.32 PEE156 pKa = 4.64 GQVITFEE163 pKa = 4.69 PEE165 pKa = 3.78 LEE167 pKa = 4.31 SLLTAHH173 pKa = 7.49 GCPTTPVADD182 pKa = 3.45 LWGRR186 pKa = 11.84 NIPYY190 pKa = 9.89 VSVYY194 pKa = 9.37 TSSIGYY200 pKa = 9.65 ALEE203 pKa = 4.28 NYY205 pKa = 9.83 RR206 pKa = 11.84 SSLFLALTRR215 pKa = 11.84 HH216 pKa = 5.26 QKK218 pKa = 9.76 QLLIFDD224 pKa = 4.73 LDD226 pKa = 3.44 ARR228 pKa = 11.84 ADD230 pKa = 3.78 PTHH233 pKa = 6.37 EE234 pKa = 4.08 LL235 pKa = 3.66
Molecular weight: 26.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.293
IPC2_protein 5.359
IPC_protein 5.347
Toseland 5.626
ProMoST 5.626
Dawson 5.525
Bjellqvist 5.563
Wikipedia 5.499
Rodwell 5.486
Grimsley 5.728
Solomon 5.525
Lehninger 5.499
Nozaki 5.753
DTASelect 5.931
Thurlkill 5.855
EMBOSS 5.817
Sillero 5.842
Patrickios 3.439
IPC_peptide 5.537
IPC2_peptide 5.855
IPC2.peptide.svr19 5.822
Protein with the highest isoelectric point:
>tr|A0A0S2ZX53|A0A0S2ZX53_9VIRU Movement protein TGBp3 OS=Actinidia virus X OX=1331744 PE=3 SV=1
MM1 pKa = 7.32 SHH3 pKa = 7.64 PDD5 pKa = 3.03 SSAATLLRR13 pKa = 11.84 LSGTCALIPALRR25 pKa = 11.84 PLTGSKK31 pKa = 8.24 WVYY34 pKa = 10.25 RR35 pKa = 11.84 SLNALLALTSLRR47 pKa = 11.84 PCSHH51 pKa = 6.46 QLLLNLKK58 pKa = 9.11 GDD60 pKa = 3.92 SYY62 pKa = 12.15 ANPLCGKK69 pKa = 9.73 SKK71 pKa = 9.08 PTLSQRR77 pKa = 11.84 LAPWPVRR84 pKa = 11.84 RR85 pKa = 11.84 FRR87 pKa = 11.84 MAITSPTT94 pKa = 3.36
Molecular weight: 10.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.304
IPC2_protein 9.926
IPC_protein 10.965
Toseland 11.067
ProMoST 11.096
Dawson 11.125
Bjellqvist 10.95
Wikipedia 11.433
Rodwell 11.228
Grimsley 11.169
Solomon 11.389
Lehninger 11.33
Nozaki 11.067
DTASelect 10.935
Thurlkill 11.067
EMBOSS 11.506
Sillero 11.082
Patrickios 10.994
IPC_peptide 11.389
IPC2_peptide 10.321
IPC2.peptide.svr19 8.575
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2376
89
1591
396.0
44.18
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.923 ± 0.482
1.726 ± 0.377
4.924 ± 0.703
5.345 ± 1.077
3.956 ± 0.576
4.882 ± 0.968
3.535 ± 0.924
5.093 ± 0.395
4.882 ± 0.786
9.68 ± 1.037
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.104 ± 0.448
3.998 ± 0.239
7.407 ± 0.789
4.209 ± 0.279
5.051 ± 0.333
6.439 ± 1.139
7.576 ± 0.445
5.766 ± 0.473
1.136 ± 0.167
3.367 ± 0.355
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here