Amino acid dipepetide frequency for Streptococcus phage phiJH1301-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.138AlaAla: 3.138 ± 0.593
0.374AlaCys: 0.374 ± 0.183
5.977AlaAsp: 5.977 ± 1.062
6.425AlaGlu: 6.425 ± 0.796
2.914AlaPhe: 2.914 ± 0.49
5.827AlaGly: 5.827 ± 0.729
0.747AlaHis: 0.747 ± 0.279
6.126AlaIle: 6.126 ± 0.748
5.827AlaLys: 5.827 ± 0.675
5.902AlaLeu: 5.902 ± 0.681
1.644AlaMet: 1.644 ± 0.397
4.258AlaAsn: 4.258 ± 0.559
2.391AlaPro: 2.391 ± 0.378
2.839AlaGln: 2.839 ± 0.411
2.465AlaArg: 2.465 ± 0.472
3.362AlaSer: 3.362 ± 0.542
4.258AlaThr: 4.258 ± 0.602
5.08AlaVal: 5.08 ± 0.611
1.121AlaTrp: 1.121 ± 0.366
3.213AlaTyr: 3.213 ± 0.581
0.0AlaXaa: 0.0 ± 0.0
Cys
0.0CysAla: 0.0 ± 0.0
0.075CysCys: 0.075 ± 0.077
0.448CysAsp: 0.448 ± 0.203
0.374CysGlu: 0.374 ± 0.175
0.299CysPhe: 0.299 ± 0.136
0.822CysGly: 0.822 ± 0.258
0.224CysHis: 0.224 ± 0.136
0.299CysIle: 0.299 ± 0.14
0.374CysLys: 0.374 ± 0.152
0.523CysLeu: 0.523 ± 0.272
0.0CysMet: 0.0 ± 0.0
0.149CysAsn: 0.149 ± 0.114
0.149CysPro: 0.149 ± 0.106
0.224CysGln: 0.224 ± 0.098
0.523CysArg: 0.523 ± 0.215
0.448CysSer: 0.448 ± 0.146
0.224CysThr: 0.224 ± 0.13
0.448CysVal: 0.448 ± 0.175
0.075CysTrp: 0.075 ± 0.063
0.149CysTyr: 0.149 ± 0.095
0.0CysXaa: 0.0 ± 0.0
Asp
5.08AspAla: 5.08 ± 0.611
0.598AspCys: 0.598 ± 0.197
3.661AspAsp: 3.661 ± 0.753
4.632AspGlu: 4.632 ± 0.694
1.942AspPhe: 1.942 ± 0.451
4.333AspGly: 4.333 ± 0.86
0.149AspHis: 0.149 ± 0.104
4.707AspIle: 4.707 ± 0.67
5.977AspLys: 5.977 ± 0.816
3.661AspLeu: 3.661 ± 0.485
1.644AspMet: 1.644 ± 0.359
3.736AspAsn: 3.736 ± 0.411
1.644AspPro: 1.644 ± 0.368
0.897AspGln: 0.897 ± 0.318
2.54AspArg: 2.54 ± 0.402
4.184AspSer: 4.184 ± 0.492
3.96AspThr: 3.96 ± 0.542
5.006AspVal: 5.006 ± 0.519
1.195AspTrp: 1.195 ± 0.263
3.063AspTyr: 3.063 ± 0.518
0.0AspXaa: 0.0 ± 0.0
Glu
4.856GluAla: 4.856 ± 0.665
0.448GluCys: 0.448 ± 0.186
3.213GluAsp: 3.213 ± 0.529
6.724GluGlu: 6.724 ± 1.117
3.138GluPhe: 3.138 ± 0.546
4.333GluGly: 4.333 ± 0.537
1.046GluHis: 1.046 ± 0.306
6.276GluIle: 6.276 ± 0.674
5.902GluLys: 5.902 ± 0.812
7.471GluLeu: 7.471 ± 0.982
2.017GluMet: 2.017 ± 0.446
3.96GluAsn: 3.96 ± 0.531
2.69GluPro: 2.69 ± 0.436
4.483GluGln: 4.483 ± 0.785
4.483GluArg: 4.483 ± 0.643
3.736GluSer: 3.736 ± 0.41
3.213GluThr: 3.213 ± 0.416
5.603GluVal: 5.603 ± 0.606
1.121GluTrp: 1.121 ± 0.269
2.241GluTyr: 2.241 ± 0.474
0.0GluXaa: 0.0 ± 0.0
Phe
2.914PheAla: 2.914 ± 0.422
0.224PheCys: 0.224 ± 0.131
3.138PheAsp: 3.138 ± 0.579
2.615PheGlu: 2.615 ± 0.432
1.569PhePhe: 1.569 ± 0.298
3.511PheGly: 3.511 ± 0.858
0.672PheHis: 0.672 ± 0.265
1.868PheIle: 1.868 ± 0.407
2.465PheLys: 2.465 ± 0.582
2.391PheLeu: 2.391 ± 0.398
0.598PheMet: 0.598 ± 0.189
1.942PheAsn: 1.942 ± 0.361
1.494PhePro: 1.494 ± 0.496
1.195PheGln: 1.195 ± 0.369
1.793PheArg: 1.793 ± 0.372
2.316PheSer: 2.316 ± 0.494
3.213PheThr: 3.213 ± 0.415
3.138PheVal: 3.138 ± 0.493
0.448PheTrp: 0.448 ± 0.171
1.718PheTyr: 1.718 ± 0.34
0.0PheXaa: 0.0 ± 0.0
Gly
4.707GlyAla: 4.707 ± 0.634
0.224GlyCys: 0.224 ± 0.111
3.586GlyAsp: 3.586 ± 0.586
4.184GlyGlu: 4.184 ± 0.454
2.988GlyPhe: 2.988 ± 0.44
5.155GlyGly: 5.155 ± 1.037
0.822GlyHis: 0.822 ± 0.262
5.304GlyIle: 5.304 ± 0.888
4.707GlyLys: 4.707 ± 0.523
5.603GlyLeu: 5.603 ± 0.753
1.868GlyMet: 1.868 ± 0.489
3.287GlyAsn: 3.287 ± 0.452
0.822GlyPro: 0.822 ± 0.273
3.362GlyGln: 3.362 ± 0.589
4.483GlyArg: 4.483 ± 0.61
3.511GlySer: 3.511 ± 0.739
3.81GlyThr: 3.81 ± 0.825
5.155GlyVal: 5.155 ± 0.613
0.747GlyTrp: 0.747 ± 0.279
2.391GlyTyr: 2.391 ± 0.383
0.0GlyXaa: 0.0 ± 0.0
His
0.897HisAla: 0.897 ± 0.304
0.149HisCys: 0.149 ± 0.106
0.971HisAsp: 0.971 ± 0.311
1.345HisGlu: 1.345 ± 0.322
0.747HisPhe: 0.747 ± 0.283
1.419HisGly: 1.419 ± 0.318
0.224HisHis: 0.224 ± 0.127
0.897HisIle: 0.897 ± 0.286
0.822HisLys: 0.822 ± 0.239
1.27HisLeu: 1.27 ± 0.292
0.299HisMet: 0.299 ± 0.212
0.747HisAsn: 0.747 ± 0.206
0.374HisPro: 0.374 ± 0.156
0.374HisGln: 0.374 ± 0.155
0.448HisArg: 0.448 ± 0.213
0.598HisSer: 0.598 ± 0.294
0.598HisThr: 0.598 ± 0.198
0.822HisVal: 0.822 ± 0.214
0.149HisTrp: 0.149 ± 0.095
0.523HisTyr: 0.523 ± 0.151
0.0HisXaa: 0.0 ± 0.0
Ile
5.08IleAla: 5.08 ± 0.56
0.523IleCys: 0.523 ± 0.192
4.258IleAsp: 4.258 ± 0.455
6.276IleGlu: 6.276 ± 0.728
2.465IlePhe: 2.465 ± 0.585
3.736IleGly: 3.736 ± 0.879
0.971IleHis: 0.971 ± 0.286
4.109IleIle: 4.109 ± 0.691
6.052IleLys: 6.052 ± 0.611
5.379IleLeu: 5.379 ± 0.752
1.27IleMet: 1.27 ± 0.252
3.138IleAsn: 3.138 ± 0.444
1.718IlePro: 1.718 ± 0.398
2.241IleGln: 2.241 ± 0.418
3.885IleArg: 3.885 ± 0.525
4.333IleSer: 4.333 ± 0.76
3.661IleThr: 3.661 ± 0.471
3.437IleVal: 3.437 ± 0.569
1.046IleTrp: 1.046 ± 0.302
3.213IleTyr: 3.213 ± 0.636
0.0IleXaa: 0.0 ± 0.0
Lys
6.052LysAla: 6.052 ± 0.753
0.224LysCys: 0.224 ± 0.136
4.333LysAsp: 4.333 ± 0.579
6.126LysGlu: 6.126 ± 0.785
2.092LysPhe: 2.092 ± 0.336
4.408LysGly: 4.408 ± 0.619
1.195LysHis: 1.195 ± 0.354
4.856LysIle: 4.856 ± 0.612
6.201LysLys: 6.201 ± 0.972
6.948LysLeu: 6.948 ± 0.618
2.167LysMet: 2.167 ± 0.561
3.96LysAsn: 3.96 ± 0.645
2.54LysPro: 2.54 ± 0.496
3.213LysGln: 3.213 ± 0.533
3.81LysArg: 3.81 ± 0.56
4.557LysSer: 4.557 ± 0.511
5.379LysThr: 5.379 ± 0.583
5.977LysVal: 5.977 ± 0.57
1.195LysTrp: 1.195 ± 0.314
2.988LysTyr: 2.988 ± 0.555
0.0LysXaa: 0.0 ± 0.0
Leu
7.247LeuAla: 7.247 ± 0.843
0.299LeuCys: 0.299 ± 0.174
5.603LeuAsp: 5.603 ± 0.684
6.948LeuGlu: 6.948 ± 0.792
2.914LeuPhe: 2.914 ± 0.539
5.753LeuGly: 5.753 ± 0.678
0.897LeuHis: 0.897 ± 0.239
3.661LeuIle: 3.661 ± 0.465
6.276LeuLys: 6.276 ± 0.694
6.35LeuLeu: 6.35 ± 0.644
1.494LeuMet: 1.494 ± 0.304
3.96LeuAsn: 3.96 ± 0.76
2.988LeuPro: 2.988 ± 0.553
3.138LeuGln: 3.138 ± 0.52
3.81LeuArg: 3.81 ± 0.659
5.006LeuSer: 5.006 ± 0.554
5.304LeuThr: 5.304 ± 0.719
5.977LeuVal: 5.977 ± 0.697
0.672LeuTrp: 0.672 ± 0.255
2.69LeuTyr: 2.69 ± 0.471
0.0LeuXaa: 0.0 ± 0.0
Met
1.494MetAla: 1.494 ± 0.418
0.0MetCys: 0.0 ± 0.0
1.195MetAsp: 1.195 ± 0.241
1.419MetGlu: 1.419 ± 0.308
1.27MetPhe: 1.27 ± 0.286
1.718MetGly: 1.718 ± 0.345
0.149MetHis: 0.149 ± 0.101
1.345MetIle: 1.345 ± 0.328
2.167MetLys: 2.167 ± 0.455
2.167MetLeu: 2.167 ± 0.333
0.374MetMet: 0.374 ± 0.165
1.046MetAsn: 1.046 ± 0.299
0.448MetPro: 0.448 ± 0.208
0.822MetGln: 0.822 ± 0.271
0.523MetArg: 0.523 ± 0.221
2.092MetSer: 2.092 ± 0.385
2.465MetThr: 2.465 ± 0.525
1.046MetVal: 1.046 ± 0.257
0.0MetTrp: 0.0 ± 0.0
0.374MetTyr: 0.374 ± 0.156
0.0MetXaa: 0.0 ± 0.0
Asn
3.885AsnAla: 3.885 ± 0.773
0.299AsnCys: 0.299 ± 0.117
3.661AsnAsp: 3.661 ± 0.412
3.362AsnGlu: 3.362 ± 0.585
1.718AsnPhe: 1.718 ± 0.36
3.736AsnGly: 3.736 ± 0.616
1.046AsnHis: 1.046 ± 0.206
2.988AsnIle: 2.988 ± 0.742
3.661AsnLys: 3.661 ± 0.458
4.557AsnLeu: 4.557 ± 0.658
1.046AsnMet: 1.046 ± 0.258
2.54AsnAsn: 2.54 ± 0.545
1.868AsnPro: 1.868 ± 0.49
1.942AsnGln: 1.942 ± 0.475
3.362AsnArg: 3.362 ± 0.511
2.465AsnSer: 2.465 ± 0.421
2.914AsnThr: 2.914 ± 0.364
2.914AsnVal: 2.914 ± 0.398
0.897AsnTrp: 0.897 ± 0.287
2.092AsnTyr: 2.092 ± 0.416
0.0AsnXaa: 0.0 ± 0.0
Pro
2.391ProAla: 2.391 ± 0.415
0.149ProCys: 0.149 ± 0.094
1.942ProAsp: 1.942 ± 0.318
2.391ProGlu: 2.391 ± 0.373
0.897ProPhe: 0.897 ± 0.202
1.345ProGly: 1.345 ± 0.492
0.672ProHis: 0.672 ± 0.239
2.316ProIle: 2.316 ± 0.324
3.138ProLys: 3.138 ± 0.594
2.017ProLeu: 2.017 ± 0.374
0.598ProMet: 0.598 ± 0.174
2.316ProAsn: 2.316 ± 0.333
0.747ProPro: 0.747 ± 0.258
1.345ProGln: 1.345 ± 0.322
1.345ProArg: 1.345 ± 0.393
1.419ProSer: 1.419 ± 0.41
2.092ProThr: 2.092 ± 0.322
1.569ProVal: 1.569 ± 0.35
0.598ProTrp: 0.598 ± 0.246
1.195ProTyr: 1.195 ± 0.285
0.0ProXaa: 0.0 ± 0.0
Gln
4.557GlnAla: 4.557 ± 0.556
0.224GlnCys: 0.224 ± 0.113
1.569GlnAsp: 1.569 ± 0.339
3.138GlnGlu: 3.138 ± 0.526
2.092GlnPhe: 2.092 ± 0.352
2.017GlnGly: 2.017 ± 0.455
0.523GlnHis: 0.523 ± 0.219
3.063GlnIle: 3.063 ± 0.397
3.138GlnLys: 3.138 ± 0.476
3.063GlnLeu: 3.063 ± 0.391
1.046GlnMet: 1.046 ± 0.316
2.54GlnAsn: 2.54 ± 0.398
1.195GlnPro: 1.195 ± 0.284
2.017GlnGln: 2.017 ± 0.391
1.569GlnArg: 1.569 ± 0.341
2.241GlnSer: 2.241 ± 0.432
2.391GlnThr: 2.391 ± 0.333
2.167GlnVal: 2.167 ± 0.357
0.747GlnTrp: 0.747 ± 0.231
1.195GlnTyr: 1.195 ± 0.308
0.0GlnXaa: 0.0 ± 0.0
Arg
3.437ArgAla: 3.437 ± 0.526
0.299ArgCys: 0.299 ± 0.162
2.839ArgAsp: 2.839 ± 0.422
3.063ArgGlu: 3.063 ± 0.531
2.241ArgPhe: 2.241 ± 0.439
2.839ArgGly: 2.839 ± 0.546
0.747ArgHis: 0.747 ± 0.259
3.96ArgIle: 3.96 ± 0.47
3.736ArgLys: 3.736 ± 0.722
4.632ArgLeu: 4.632 ± 0.776
1.195ArgMet: 1.195 ± 0.289
2.465ArgAsn: 2.465 ± 0.376
1.494ArgPro: 1.494 ± 0.388
1.569ArgGln: 1.569 ± 0.294
2.241ArgArg: 2.241 ± 0.495
3.213ArgSer: 3.213 ± 0.528
2.914ArgThr: 2.914 ± 0.476
2.764ArgVal: 2.764 ± 0.586
0.971ArgTrp: 0.971 ± 0.291
2.017ArgTyr: 2.017 ± 0.39
0.0ArgXaa: 0.0 ± 0.0
Ser
4.707SerAla: 4.707 ± 0.542
0.149SerCys: 0.149 ± 0.099
4.184SerAsp: 4.184 ± 0.503
4.258SerGlu: 4.258 ± 0.621
2.241SerPhe: 2.241 ± 0.654
4.333SerGly: 4.333 ± 0.721
0.523SerHis: 0.523 ± 0.193
3.96SerIle: 3.96 ± 0.525
4.109SerLys: 4.109 ± 0.496
5.08SerLeu: 5.08 ± 0.719
1.121SerMet: 1.121 ± 0.239
2.914SerAsn: 2.914 ± 0.582
1.419SerPro: 1.419 ± 0.315
2.764SerGln: 2.764 ± 0.63
2.988SerArg: 2.988 ± 0.577
3.885SerSer: 3.885 ± 0.856
3.661SerThr: 3.661 ± 0.719
3.362SerVal: 3.362 ± 0.626
0.598SerTrp: 0.598 ± 0.253
2.764SerTyr: 2.764 ± 0.489
0.0SerXaa: 0.0 ± 0.0
Thr
4.707ThrAla: 4.707 ± 0.53
0.374ThrCys: 0.374 ± 0.158
4.408ThrAsp: 4.408 ± 0.563
3.81ThrGlu: 3.81 ± 0.494
2.69ThrPhe: 2.69 ± 0.453
4.931ThrGly: 4.931 ± 0.708
0.747ThrHis: 0.747 ± 0.239
4.184ThrIle: 4.184 ± 0.418
4.856ThrLys: 4.856 ± 0.75
4.109ThrLeu: 4.109 ± 0.57
1.868ThrMet: 1.868 ± 0.415
2.092ThrAsn: 2.092 ± 0.387
1.868ThrPro: 1.868 ± 0.318
2.391ThrGln: 2.391 ± 0.406
1.942ThrArg: 1.942 ± 0.378
4.034ThrSer: 4.034 ± 0.614
4.931ThrThr: 4.931 ± 0.597
4.931ThrVal: 4.931 ± 0.738
1.195ThrTrp: 1.195 ± 0.299
2.167ThrTyr: 2.167 ± 0.631
0.0ThrXaa: 0.0 ± 0.0
Val
6.052ValAla: 6.052 ± 0.589
0.672ValCys: 0.672 ± 0.236
4.258ValAsp: 4.258 ± 0.478
6.35ValGlu: 6.35 ± 0.704
2.092ValPhe: 2.092 ± 0.467
3.96ValGly: 3.96 ± 0.632
1.046ValHis: 1.046 ± 0.288
3.362ValIle: 3.362 ± 0.536
4.781ValLys: 4.781 ± 0.519
5.304ValLeu: 5.304 ± 0.559
1.121ValMet: 1.121 ± 0.256
3.213ValAsn: 3.213 ± 0.413
3.138ValPro: 3.138 ± 0.466
2.615ValGln: 2.615 ± 0.472
3.586ValArg: 3.586 ± 0.54
4.557ValSer: 4.557 ± 0.814
3.661ValThr: 3.661 ± 0.529
5.155ValVal: 5.155 ± 0.652
0.747ValTrp: 0.747 ± 0.208
2.54ValTyr: 2.54 ± 0.449
0.0ValXaa: 0.0 ± 0.0
Trp
0.672TrpAla: 0.672 ± 0.296
0.149TrpCys: 0.149 ± 0.121
0.672TrpAsp: 0.672 ± 0.343
0.971TrpGlu: 0.971 ± 0.294
1.27TrpPhe: 1.27 ± 0.624
0.374TrpGly: 0.374 ± 0.153
0.149TrpHis: 0.149 ± 0.112
0.897TrpIle: 0.897 ± 0.253
1.195TrpLys: 1.195 ± 0.29
1.27TrpLeu: 1.27 ± 0.281
0.075TrpMet: 0.075 ± 0.07
0.897TrpAsn: 0.897 ± 0.402
0.075TrpPro: 0.075 ± 0.063
1.046TrpGln: 1.046 ± 0.277
0.598TrpArg: 0.598 ± 0.221
0.897TrpSer: 0.897 ± 0.28
1.27TrpThr: 1.27 ± 0.372
1.046TrpVal: 1.046 ± 0.306
0.224TrpTrp: 0.224 ± 0.138
0.374TrpTyr: 0.374 ± 0.149
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.017TyrAla: 2.017 ± 0.316
0.374TyrCys: 0.374 ± 0.162
2.69TyrAsp: 2.69 ± 0.596
2.54TyrGlu: 2.54 ± 0.552
1.644TyrPhe: 1.644 ± 0.332
2.241TyrGly: 2.241 ± 0.326
1.121TyrHis: 1.121 ± 0.277
2.615TyrIle: 2.615 ± 0.616
2.839TyrLys: 2.839 ± 0.528
3.287TyrLeu: 3.287 ± 0.559
0.598TyrMet: 0.598 ± 0.217
1.793TyrAsn: 1.793 ± 0.343
1.419TyrPro: 1.419 ± 0.318
2.017TyrGln: 2.017 ± 0.465
2.241TyrArg: 2.241 ± 0.43
2.167TyrSer: 2.167 ± 0.518
2.316TyrThr: 2.316 ± 0.457
2.615TyrVal: 2.615 ± 0.433
0.374TyrTrp: 0.374 ± 0.213
1.942TyrTyr: 1.942 ± 0.46
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 72 proteins (13386 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski