bacterium 1XD8-76

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3768 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3A9EV71|A0A3A9EV71_9BACT Uncharacterized protein OS=bacterium 1XD8-76 OX=2320115 GN=D7X98_19615 PE=4 SV=1
MM1 pKa = 7.61KK2 pKa = 10.44KK3 pKa = 10.24LFSVTLTGVVAFGLIACGNNAEE25 pKa = 4.18EE26 pKa = 4.68APAEE30 pKa = 4.04EE31 pKa = 4.45SSRR34 pKa = 11.84TEE36 pKa = 3.84SAEE39 pKa = 4.05EE40 pKa = 4.0PGSEE44 pKa = 4.08AEE46 pKa = 4.33EE47 pKa = 4.26PASEE51 pKa = 3.96EE52 pKa = 4.17AEE54 pKa = 4.19GGEE57 pKa = 4.35EE58 pKa = 4.93VTLEE62 pKa = 3.97WQQWWAVEE70 pKa = 4.0APEE73 pKa = 5.82GYY75 pKa = 8.62VQNMVDD81 pKa = 3.68KK82 pKa = 9.93YY83 pKa = 10.44YY84 pKa = 10.7EE85 pKa = 4.0EE86 pKa = 3.98TGVRR90 pKa = 11.84VEE92 pKa = 4.44LLSAPFADD100 pKa = 3.58TKK102 pKa = 8.88TQIVSGVSTGTVADD116 pKa = 3.63IVSVDD121 pKa = 3.58GSWVYY126 pKa = 11.45DD127 pKa = 3.81FADD130 pKa = 3.42QGVLTNMSTLMDD142 pKa = 4.62SIGFDD147 pKa = 3.04KK148 pKa = 10.87SLPASTWDD156 pKa = 3.79VNGSTYY162 pKa = 10.95AVPVLNFAYY171 pKa = 9.85PMFANQDD178 pKa = 3.38ILDD181 pKa = 4.08EE182 pKa = 4.85CGVTEE187 pKa = 5.73LPKK190 pKa = 9.41TWSDD194 pKa = 3.88FEE196 pKa = 4.44EE197 pKa = 4.68VCQKK201 pKa = 10.42IVDD204 pKa = 3.61KK205 pKa = 11.09GYY207 pKa = 10.91YY208 pKa = 9.57PYY210 pKa = 11.46ALNLDD215 pKa = 3.81TSSPSGIQNVYY226 pKa = 9.91MGFAWASDD234 pKa = 2.45IKK236 pKa = 10.63MKK238 pKa = 11.13NEE240 pKa = 4.34DD241 pKa = 3.55GNFDD245 pKa = 3.68VVNNPEE251 pKa = 4.08LKK253 pKa = 10.37EE254 pKa = 3.62YY255 pKa = 11.21AEE257 pKa = 4.67WIKK260 pKa = 11.09SLNDD264 pKa = 2.99KK265 pKa = 10.99GYY267 pKa = 9.19IYY269 pKa = 10.48PGMSSLTEE277 pKa = 3.52TDD279 pKa = 3.18MTSKK283 pKa = 10.51FSSGNICFIINSAAAITAWRR303 pKa = 11.84ADD305 pKa = 3.71APDD308 pKa = 4.34LNLTAAPIPVKK319 pKa = 10.76DD320 pKa = 4.23DD321 pKa = 3.48FTGEE325 pKa = 3.98SGMCVANWSLGITEE339 pKa = 4.11NSEE342 pKa = 4.13HH343 pKa = 7.26KK344 pKa = 10.44EE345 pKa = 3.6EE346 pKa = 3.94AMKK349 pKa = 10.71FIEE352 pKa = 4.19WLLNADD358 pKa = 4.45DD359 pKa = 5.38GGVCADD365 pKa = 4.34LAVTQTAFPNSTLAKK380 pKa = 9.16PDD382 pKa = 3.67YY383 pKa = 11.18SNGDD387 pKa = 3.44PVFQDD392 pKa = 3.19VYY394 pKa = 11.94AMYY397 pKa = 10.58QSGYY401 pKa = 9.17PYY403 pKa = 11.22NEE405 pKa = 3.63FTGMKK410 pKa = 8.8EE411 pKa = 3.99ANTIMTDD418 pKa = 3.78YY419 pKa = 10.72IDD421 pKa = 4.25EE422 pKa = 4.69LVTYY426 pKa = 9.55MDD428 pKa = 4.88GDD430 pKa = 3.93TDD432 pKa = 3.36VDD434 pKa = 3.75TFMSNLQEE442 pKa = 4.33TLDD445 pKa = 3.79EE446 pKa = 4.77VYY448 pKa = 11.08GKK450 pKa = 10.58

Molecular weight: 49.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3A9FKK9|A0A3A9FKK9_9BACT DeoR/GlpR transcriptional regulator OS=bacterium 1XD8-76 OX=2320115 GN=D7X98_01990 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.95KK9 pKa = 7.58RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.17VHH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTPGGRR28 pKa = 11.84KK29 pKa = 8.71VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.89RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3768

0

3768

1202740

26

1765

319.2

35.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.739 ± 0.042

1.586 ± 0.015

5.466 ± 0.03

8.318 ± 0.048

4.177 ± 0.031

7.424 ± 0.038

1.613 ± 0.014

7.034 ± 0.038

6.384 ± 0.038

9.052 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.183 ± 0.02

3.998 ± 0.028

3.246 ± 0.022

3.088 ± 0.02

5.122 ± 0.036

5.591 ± 0.031

4.978 ± 0.026

6.783 ± 0.033

0.996 ± 0.015

4.222 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski