Amino acid dipepetide frequency for Escherichia phage DY1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.786AlaAla: 9.786 ± 0.945
0.829AlaCys: 0.829 ± 0.255
5.391AlaAsp: 5.391 ± 0.711
5.971AlaGlu: 5.971 ± 0.655
3.4AlaPhe: 3.4 ± 0.407
7.464AlaGly: 7.464 ± 1.13
0.663AlaHis: 0.663 ± 0.226
4.976AlaIle: 4.976 ± 0.666
5.805AlaLys: 5.805 ± 0.676
6.718AlaLeu: 6.718 ± 0.926
2.82AlaMet: 2.82 ± 0.451
3.483AlaAsn: 3.483 ± 0.518
2.82AlaPro: 2.82 ± 0.581
2.986AlaGln: 2.986 ± 0.513
3.732AlaArg: 3.732 ± 0.512
4.23AlaSer: 4.23 ± 0.518
4.147AlaThr: 4.147 ± 0.692
5.722AlaVal: 5.722 ± 0.914
1.244AlaTrp: 1.244 ± 0.399
2.654AlaTyr: 2.654 ± 0.456
0.0AlaXaa: 0.0 ± 0.0
Cys
0.415CysAla: 0.415 ± 0.163
0.249CysCys: 0.249 ± 0.2
0.995CysAsp: 0.995 ± 0.345
0.746CysGlu: 0.746 ± 0.24
0.498CysPhe: 0.498 ± 0.214
0.746CysGly: 0.746 ± 0.252
0.332CysHis: 0.332 ± 0.159
0.415CysIle: 0.415 ± 0.219
0.829CysLys: 0.829 ± 0.246
0.995CysLeu: 0.995 ± 0.331
0.332CysMet: 0.332 ± 0.188
0.415CysAsn: 0.415 ± 0.191
0.332CysPro: 0.332 ± 0.186
0.166CysGln: 0.166 ± 0.129
0.581CysArg: 0.581 ± 0.174
0.663CysSer: 0.663 ± 0.252
0.249CysThr: 0.249 ± 0.134
0.663CysVal: 0.663 ± 0.245
0.249CysTrp: 0.249 ± 0.138
0.415CysTyr: 0.415 ± 0.241
0.0CysXaa: 0.0 ± 0.0
Asp
6.386AspAla: 6.386 ± 0.792
0.912AspCys: 0.912 ± 0.364
4.644AspAsp: 4.644 ± 0.758
3.234AspGlu: 3.234 ± 0.478
2.322AspPhe: 2.322 ± 0.471
6.883AspGly: 6.883 ± 0.783
1.493AspHis: 1.493 ± 0.356
3.566AspIle: 3.566 ± 0.528
3.566AspLys: 3.566 ± 0.669
5.225AspLeu: 5.225 ± 0.648
2.073AspMet: 2.073 ± 0.405
2.903AspAsn: 2.903 ± 0.615
2.571AspPro: 2.571 ± 0.583
1.659AspGln: 1.659 ± 0.44
1.99AspArg: 1.99 ± 0.344
3.649AspSer: 3.649 ± 0.422
3.649AspThr: 3.649 ± 0.526
4.395AspVal: 4.395 ± 0.593
0.995AspTrp: 0.995 ± 0.298
2.405AspTyr: 2.405 ± 0.448
0.0AspXaa: 0.0 ± 0.0
Glu
6.8GluAla: 6.8 ± 1.067
0.746GluCys: 0.746 ± 0.217
5.059GluAsp: 5.059 ± 0.711
4.312GluGlu: 4.312 ± 0.8
2.488GluPhe: 2.488 ± 0.451
4.561GluGly: 4.561 ± 0.847
1.244GluHis: 1.244 ± 0.294
2.654GluIle: 2.654 ± 0.475
2.903GluLys: 2.903 ± 0.448
5.556GluLeu: 5.556 ± 0.657
2.322GluMet: 2.322 ± 0.405
2.322GluAsn: 2.322 ± 0.577
2.073GluPro: 2.073 ± 0.408
2.903GluGln: 2.903 ± 0.459
4.395GluArg: 4.395 ± 0.701
3.4GluSer: 3.4 ± 0.599
3.732GluThr: 3.732 ± 0.428
4.561GluVal: 4.561 ± 0.658
1.742GluTrp: 1.742 ± 0.311
2.654GluTyr: 2.654 ± 0.479
0.0GluXaa: 0.0 ± 0.0
Phe
2.903PheAla: 2.903 ± 0.44
0.332PheCys: 0.332 ± 0.176
2.654PheAsp: 2.654 ± 0.408
1.825PheGlu: 1.825 ± 0.325
0.995PhePhe: 0.995 ± 0.346
2.654PheGly: 2.654 ± 0.498
0.829PheHis: 0.829 ± 0.245
1.493PheIle: 1.493 ± 0.467
3.151PheLys: 3.151 ± 0.557
2.903PheLeu: 2.903 ± 0.399
0.995PheMet: 0.995 ± 0.239
2.239PheAsn: 2.239 ± 0.346
1.576PhePro: 1.576 ± 0.463
0.829PheGln: 0.829 ± 0.313
1.576PheArg: 1.576 ± 0.326
2.571PheSer: 2.571 ± 0.307
1.907PheThr: 1.907 ± 0.41
2.654PheVal: 2.654 ± 0.47
0.249PheTrp: 0.249 ± 0.103
1.327PheTyr: 1.327 ± 0.266
0.0PheXaa: 0.0 ± 0.0
Gly
6.303GlyAla: 6.303 ± 0.942
0.581GlyCys: 0.581 ± 0.28
4.81GlyAsp: 4.81 ± 0.881
5.059GlyGlu: 5.059 ± 0.505
2.156GlyPhe: 2.156 ± 0.395
5.391GlyGly: 5.391 ± 0.581
0.995GlyHis: 0.995 ± 0.303
4.395GlyIle: 4.395 ± 0.544
6.054GlyLys: 6.054 ± 1.024
6.054GlyLeu: 6.054 ± 0.729
2.405GlyMet: 2.405 ± 0.417
2.82GlyAsn: 2.82 ± 0.669
1.327GlyPro: 1.327 ± 0.311
2.571GlyGln: 2.571 ± 0.463
5.639GlyArg: 5.639 ± 0.698
6.22GlySer: 6.22 ± 0.586
4.644GlyThr: 4.644 ± 0.576
6.22GlyVal: 6.22 ± 0.665
1.078GlyTrp: 1.078 ± 0.276
4.147GlyTyr: 4.147 ± 0.491
0.0GlyXaa: 0.0 ± 0.0
His
0.746HisAla: 0.746 ± 0.28
0.166HisCys: 0.166 ± 0.114
1.493HisAsp: 1.493 ± 0.519
1.493HisGlu: 1.493 ± 0.511
0.498HisPhe: 0.498 ± 0.244
1.244HisGly: 1.244 ± 0.262
0.498HisHis: 0.498 ± 0.269
1.161HisIle: 1.161 ± 0.242
1.493HisLys: 1.493 ± 0.342
1.742HisLeu: 1.742 ± 0.411
0.581HisMet: 0.581 ± 0.211
0.498HisAsn: 0.498 ± 0.208
0.415HisPro: 0.415 ± 0.185
0.663HisGln: 0.663 ± 0.212
1.244HisArg: 1.244 ± 0.276
0.912HisSer: 0.912 ± 0.276
1.078HisThr: 1.078 ± 0.301
1.078HisVal: 1.078 ± 0.235
0.249HisTrp: 0.249 ± 0.144
0.415HisTyr: 0.415 ± 0.172
0.0HisXaa: 0.0 ± 0.0
Ile
3.649IleAla: 3.649 ± 0.598
0.581IleCys: 0.581 ± 0.258
3.069IleAsp: 3.069 ± 0.462
2.986IleGlu: 2.986 ± 0.369
1.327IlePhe: 1.327 ± 0.34
4.064IleGly: 4.064 ± 0.499
1.161IleHis: 1.161 ± 0.33
1.907IleIle: 1.907 ± 0.386
3.981IleLys: 3.981 ± 0.648
3.317IleLeu: 3.317 ± 0.451
0.746IleMet: 0.746 ± 0.221
2.82IleAsn: 2.82 ± 0.568
2.239IlePro: 2.239 ± 0.546
1.742IleGln: 1.742 ± 0.532
2.82IleArg: 2.82 ± 0.538
3.317IleSer: 3.317 ± 0.563
3.483IleThr: 3.483 ± 0.517
4.312IleVal: 4.312 ± 0.468
0.581IleTrp: 0.581 ± 0.216
1.493IleTyr: 1.493 ± 0.242
0.0IleXaa: 0.0 ± 0.0
Lys
6.883LysAla: 6.883 ± 0.885
0.581LysCys: 0.581 ± 0.227
4.644LysAsp: 4.644 ± 0.535
4.23LysGlu: 4.23 ± 0.588
2.073LysPhe: 2.073 ± 0.491
4.478LysGly: 4.478 ± 0.594
1.327LysHis: 1.327 ± 0.41
2.322LysIle: 2.322 ± 0.369
4.064LysLys: 4.064 ± 0.964
5.474LysLeu: 5.474 ± 0.662
2.239LysMet: 2.239 ± 0.357
2.488LysAsn: 2.488 ± 0.484
2.737LysPro: 2.737 ± 0.634
2.073LysGln: 2.073 ± 0.504
4.976LysArg: 4.976 ± 0.871
3.815LysSer: 3.815 ± 0.491
3.981LysThr: 3.981 ± 0.478
5.142LysVal: 5.142 ± 0.784
0.995LysTrp: 0.995 ± 0.32
1.907LysTyr: 1.907 ± 0.383
0.0LysXaa: 0.0 ± 0.0
Leu
6.054LeuAla: 6.054 ± 0.809
0.581LeuCys: 0.581 ± 0.281
3.981LeuAsp: 3.981 ± 0.454
6.22LeuGlu: 6.22 ± 0.817
2.156LeuPhe: 2.156 ± 0.371
5.142LeuGly: 5.142 ± 0.79
1.161LeuHis: 1.161 ± 0.338
3.649LeuIle: 3.649 ± 0.591
6.469LeuLys: 6.469 ± 0.802
4.893LeuLeu: 4.893 ± 0.648
2.737LeuMet: 2.737 ± 0.51
4.727LeuAsn: 4.727 ± 0.574
3.234LeuPro: 3.234 ± 0.449
3.815LeuGln: 3.815 ± 0.558
4.81LeuArg: 4.81 ± 0.421
5.391LeuSer: 5.391 ± 0.664
5.308LeuThr: 5.308 ± 0.678
4.976LeuVal: 4.976 ± 0.602
0.912LeuTrp: 0.912 ± 0.265
2.156LeuTyr: 2.156 ± 0.429
0.0LeuXaa: 0.0 ± 0.0
Met
3.4MetAla: 3.4 ± 0.494
0.332MetCys: 0.332 ± 0.165
1.493MetAsp: 1.493 ± 0.298
2.239MetGlu: 2.239 ± 0.42
1.493MetPhe: 1.493 ± 0.367
2.156MetGly: 2.156 ± 0.446
0.249MetHis: 0.249 ± 0.145
1.493MetIle: 1.493 ± 0.261
1.161MetLys: 1.161 ± 0.282
2.571MetLeu: 2.571 ± 0.403
0.663MetMet: 0.663 ± 0.217
1.078MetAsn: 1.078 ± 0.235
0.995MetPro: 0.995 ± 0.297
0.829MetGln: 0.829 ± 0.339
1.244MetArg: 1.244 ± 0.328
2.073MetSer: 2.073 ± 0.377
1.907MetThr: 1.907 ± 0.383
2.488MetVal: 2.488 ± 0.52
0.249MetTrp: 0.249 ± 0.16
1.161MetTyr: 1.161 ± 0.254
0.0MetXaa: 0.0 ± 0.0
Asn
4.727AsnAla: 4.727 ± 0.595
0.663AsnCys: 0.663 ± 0.27
1.99AsnAsp: 1.99 ± 0.478
2.903AsnGlu: 2.903 ± 0.513
1.244AsnPhe: 1.244 ± 0.214
4.727AsnGly: 4.727 ± 0.592
0.746AsnHis: 0.746 ± 0.211
2.986AsnIle: 2.986 ± 0.835
2.737AsnLys: 2.737 ± 0.467
3.151AsnLeu: 3.151 ± 0.496
0.995AsnMet: 0.995 ± 0.276
2.405AsnAsn: 2.405 ± 0.673
3.566AsnPro: 3.566 ± 0.672
1.493AsnGln: 1.493 ± 0.323
2.156AsnArg: 2.156 ± 0.516
3.234AsnSer: 3.234 ± 1.106
2.073AsnThr: 2.073 ± 0.451
3.151AsnVal: 3.151 ± 0.626
0.166AsnTrp: 0.166 ± 0.115
1.493AsnTyr: 1.493 ± 0.405
0.0AsnXaa: 0.0 ± 0.0
Pro
3.151ProAla: 3.151 ± 0.629
0.498ProCys: 0.498 ± 0.241
2.737ProAsp: 2.737 ± 0.378
2.903ProGlu: 2.903 ± 0.597
1.161ProPhe: 1.161 ± 0.297
1.742ProGly: 1.742 ± 0.316
0.581ProHis: 0.581 ± 0.167
2.156ProIle: 2.156 ± 0.35
2.986ProLys: 2.986 ± 0.483
1.907ProLeu: 1.907 ± 0.469
0.912ProMet: 0.912 ± 0.318
2.405ProAsn: 2.405 ± 0.408
0.912ProPro: 0.912 ± 0.229
1.659ProGln: 1.659 ± 0.351
1.493ProArg: 1.493 ± 0.365
2.986ProSer: 2.986 ± 0.371
3.151ProThr: 3.151 ± 0.525
2.82ProVal: 2.82 ± 0.297
0.746ProTrp: 0.746 ± 0.221
0.829ProTyr: 0.829 ± 0.209
0.0ProXaa: 0.0 ± 0.0
Gln
4.064GlnAla: 4.064 ± 0.513
0.249GlnCys: 0.249 ± 0.14
2.986GlnAsp: 2.986 ± 0.718
1.99GlnGlu: 1.99 ± 0.352
1.825GlnPhe: 1.825 ± 0.365
2.156GlnGly: 2.156 ± 0.423
0.829GlnHis: 0.829 ± 0.237
1.244GlnIle: 1.244 ± 0.333
1.99GlnLys: 1.99 ± 0.384
3.898GlnLeu: 3.898 ± 0.591
1.327GlnMet: 1.327 ± 0.379
1.161GlnAsn: 1.161 ± 0.397
1.244GlnPro: 1.244 ± 0.355
1.742GlnGln: 1.742 ± 0.536
1.99GlnArg: 1.99 ± 0.643
2.903GlnSer: 2.903 ± 0.455
2.073GlnThr: 2.073 ± 0.488
2.571GlnVal: 2.571 ± 0.403
0.663GlnTrp: 0.663 ± 0.23
1.161GlnTyr: 1.161 ± 0.364
0.0GlnXaa: 0.0 ± 0.0
Arg
3.732ArgAla: 3.732 ± 0.779
0.663ArgCys: 0.663 ± 0.235
4.23ArgAsp: 4.23 ± 0.486
4.312ArgGlu: 4.312 ± 0.597
2.903ArgPhe: 2.903 ± 0.361
4.147ArgGly: 4.147 ± 0.453
0.498ArgHis: 0.498 ± 0.22
3.483ArgIle: 3.483 ± 0.646
3.566ArgLys: 3.566 ± 0.621
5.556ArgLeu: 5.556 ± 0.757
1.41ArgMet: 1.41 ± 0.413
2.82ArgAsn: 2.82 ± 0.511
1.493ArgPro: 1.493 ± 0.303
2.737ArgGln: 2.737 ± 0.428
2.239ArgArg: 2.239 ± 0.401
3.483ArgSer: 3.483 ± 0.577
1.907ArgThr: 1.907 ± 0.341
2.737ArgVal: 2.737 ± 0.565
1.078ArgTrp: 1.078 ± 0.258
1.576ArgTyr: 1.576 ± 0.289
0.0ArgXaa: 0.0 ± 0.0
Ser
3.566SerAla: 3.566 ± 0.497
0.912SerCys: 0.912 ± 0.325
4.893SerAsp: 4.893 ± 0.545
3.317SerGlu: 3.317 ± 0.507
2.488SerPhe: 2.488 ± 0.399
5.888SerGly: 5.888 ± 0.826
1.907SerHis: 1.907 ± 0.349
2.903SerIle: 2.903 ± 0.538
3.4SerLys: 3.4 ± 0.434
3.981SerLeu: 3.981 ± 0.514
1.659SerMet: 1.659 ± 0.368
3.483SerAsn: 3.483 ± 0.616
2.903SerPro: 2.903 ± 0.45
1.99SerGln: 1.99 ± 0.411
3.815SerArg: 3.815 ± 0.622
3.898SerSer: 3.898 ± 0.587
3.234SerThr: 3.234 ± 0.687
4.478SerVal: 4.478 ± 0.615
0.746SerTrp: 0.746 ± 0.235
2.986SerTyr: 2.986 ± 0.597
0.0SerXaa: 0.0 ± 0.0
Thr
3.566ThrAla: 3.566 ± 0.925
0.332ThrCys: 0.332 ± 0.206
2.737ThrAsp: 2.737 ± 0.415
4.644ThrGlu: 4.644 ± 0.559
2.405ThrPhe: 2.405 ± 0.454
5.556ThrGly: 5.556 ± 0.708
0.995ThrHis: 0.995 ± 0.346
3.649ThrIle: 3.649 ± 0.653
3.151ThrLys: 3.151 ± 0.457
4.644ThrLeu: 4.644 ± 0.536
1.907ThrMet: 1.907 ± 0.36
2.239ThrAsn: 2.239 ± 0.69
2.903ThrPro: 2.903 ± 0.323
2.82ThrGln: 2.82 ± 0.585
2.571ThrArg: 2.571 ± 0.452
2.488ThrSer: 2.488 ± 0.433
3.815ThrThr: 3.815 ± 0.707
4.976ThrVal: 4.976 ± 0.571
0.581ThrTrp: 0.581 ± 0.247
1.41ThrTyr: 1.41 ± 0.352
0.0ThrXaa: 0.0 ± 0.0
Val
4.976ValAla: 4.976 ± 0.632
0.498ValCys: 0.498 ± 0.2
3.649ValAsp: 3.649 ± 0.609
5.059ValGlu: 5.059 ± 0.708
2.488ValPhe: 2.488 ± 0.483
5.888ValGly: 5.888 ± 0.606
1.244ValHis: 1.244 ± 0.57
2.82ValIle: 2.82 ± 0.416
5.805ValLys: 5.805 ± 0.668
5.391ValLeu: 5.391 ± 0.708
2.073ValMet: 2.073 ± 0.431
3.483ValAsn: 3.483 ± 0.557
2.903ValPro: 2.903 ± 0.449
2.986ValGln: 2.986 ± 0.553
4.147ValArg: 4.147 ± 0.612
4.561ValSer: 4.561 ± 0.501
4.478ValThr: 4.478 ± 0.671
6.137ValVal: 6.137 ± 0.821
0.746ValTrp: 0.746 ± 0.275
2.903ValTyr: 2.903 ± 0.451
0.0ValXaa: 0.0 ± 0.0
Trp
0.498TrpAla: 0.498 ± 0.144
0.332TrpCys: 0.332 ± 0.17
0.829TrpAsp: 0.829 ± 0.257
0.995TrpGlu: 0.995 ± 0.252
0.663TrpPhe: 0.663 ± 0.201
0.746TrpGly: 0.746 ± 0.232
0.498TrpHis: 0.498 ± 0.198
0.249TrpIle: 0.249 ± 0.117
1.41TrpLys: 1.41 ± 0.363
2.073TrpLeu: 2.073 ± 0.481
0.166TrpMet: 0.166 ± 0.116
0.912TrpAsn: 0.912 ± 0.25
0.166TrpPro: 0.166 ± 0.12
0.663TrpGln: 0.663 ± 0.264
0.746TrpArg: 0.746 ± 0.208
0.746TrpSer: 0.746 ± 0.383
0.581TrpThr: 0.581 ± 0.187
1.078TrpVal: 1.078 ± 0.348
0.083TrpTrp: 0.083 ± 0.078
0.498TrpTyr: 0.498 ± 0.186
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.234TyrAla: 3.234 ± 0.59
0.249TyrCys: 0.249 ± 0.142
2.239TyrAsp: 2.239 ± 0.443
1.99TyrGlu: 1.99 ± 0.542
1.078TyrPhe: 1.078 ± 0.295
3.234TyrGly: 3.234 ± 0.53
0.498TyrHis: 0.498 ± 0.236
2.073TyrIle: 2.073 ± 0.489
1.907TyrLys: 1.907 ± 0.39
2.322TyrLeu: 2.322 ± 0.405
0.829TyrMet: 0.829 ± 0.254
1.907TyrAsn: 1.907 ± 0.458
1.327TyrPro: 1.327 ± 0.404
1.742TyrGln: 1.742 ± 0.502
2.405TyrArg: 2.405 ± 0.451
1.907TyrSer: 1.907 ± 0.351
1.99TyrThr: 1.99 ± 0.362
2.073TyrVal: 2.073 ± 0.442
0.581TyrTrp: 0.581 ± 0.236
1.493TyrTyr: 1.493 ± 0.387
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 53 proteins (12059 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski