Mycobacterium virus Che9c
Average proteome isoelectric point is 6.13
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 84 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q854X6|Q854X6_9CAUD Uncharacterized protein OS=Mycobacterium virus Che9c OX=205872 GN=21 PE=4 SV=1
MM1 pKa = 7.35 LTTGGIQLSNPTNAEE16 pKa = 3.78 DD17 pKa = 3.91 YY18 pKa = 10.56 EE19 pKa = 4.62 FAFHH23 pKa = 7.12 FSVKK27 pKa = 9.52 GVKK30 pKa = 9.65 LAGPGDD36 pKa = 4.17 ADD38 pKa = 2.98 TWYY41 pKa = 10.23 PNIQYY46 pKa = 10.62 GLRR49 pKa = 11.84 GAGDD53 pKa = 4.03 EE54 pKa = 4.23 PPPLIVQAYY63 pKa = 9.66 LMNAALNPDD72 pKa = 3.87 PEE74 pKa = 4.5 SVRR77 pKa = 11.84 FSILYY82 pKa = 7.89 TDD84 pKa = 3.97 PVTWTEE90 pKa = 3.93 VSPEE94 pKa = 4.09 EE95 pKa = 4.29 VDD97 pKa = 3.63 ALPGSVEE104 pKa = 4.45 DD105 pKa = 3.7 YY106 pKa = 11.17 SFAIMGDD113 pKa = 3.54 STDD116 pKa = 3.41 EE117 pKa = 4.0 NGAQFRR123 pKa = 11.84 LGLVGDD129 pKa = 4.31 GAYY132 pKa = 10.36 GLEE135 pKa = 4.12 VSLFTLPIYY144 pKa = 10.73 LDD146 pKa = 3.68 SQDD149 pKa = 3.66 QEE151 pKa = 4.68 GAQLRR156 pKa = 11.84 NVVLAHH162 pKa = 7.12 APRR165 pKa = 11.84 INWWAWEE172 pKa = 3.87 MDD174 pKa = 3.32 AA175 pKa = 6.11
Molecular weight: 19.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.749
IPC2_protein 3.859
IPC_protein 3.821
Toseland 3.617
ProMoST 3.986
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.732
Rodwell 3.656
Grimsley 3.528
Solomon 3.795
Lehninger 3.757
Nozaki 3.923
DTASelect 4.126
Thurlkill 3.668
EMBOSS 3.745
Sillero 3.948
Patrickios 1.888
IPC_peptide 3.795
IPC2_peptide 3.923
IPC2.peptide.svr19 3.841
Protein with the highest isoelectric point:
>tr|Q854W7|Q854W7_9CAUD Uncharacterized protein OS=Mycobacterium virus Che9c OX=205872 GN=30 PE=4 SV=1
MM1 pKa = 7.05 TRR3 pKa = 11.84 HH4 pKa = 5.81 LRR6 pKa = 11.84 CLAVKK11 pKa = 10.12 LRR13 pKa = 11.84 LMKK16 pKa = 10.73 SRR18 pKa = 11.84 DD19 pKa = 3.21 VNRR22 pKa = 11.84 RR23 pKa = 11.84 IEE25 pKa = 4.12 SLGGIEE31 pKa = 3.78 TRR33 pKa = 11.84 QRR35 pKa = 11.84 GSHH38 pKa = 5.32 RR39 pKa = 11.84 RR40 pKa = 11.84 YY41 pKa = 10.13 AVVYY45 pKa = 8.42 TDD47 pKa = 3.09 KK48 pKa = 11.29 SGNEE52 pKa = 3.43 RR53 pKa = 11.84 TAFTTVQQHH62 pKa = 6.35 KK63 pKa = 9.78 GQEE66 pKa = 4.09 IPLGTLRR73 pKa = 11.84 AIQRR77 pKa = 11.84 DD78 pKa = 3.84 LEE80 pKa = 4.24 PAFGEE85 pKa = 4.63 GWLLGG90 pKa = 3.85
Molecular weight: 10.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.324
IPC2_protein 9.706
IPC_protein 10.818
Toseland 10.95
ProMoST 10.979
Dawson 11.008
Bjellqvist 10.818
Wikipedia 11.316
Rodwell 11.023
Grimsley 11.038
Solomon 11.272
Lehninger 11.213
Nozaki 10.935
DTASelect 10.818
Thurlkill 10.935
EMBOSS 11.389
Sillero 10.95
Patrickios 10.789
IPC_peptide 11.272
IPC2_peptide 10.072
IPC2.peptide.svr19 8.776
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
84
0
84
17907
44
1213
213.2
23.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.845 ± 0.452
1.033 ± 0.121
6.545 ± 0.18
5.886 ± 0.245
2.87 ± 0.126
8.74 ± 0.489
2.178 ± 0.2
4.322 ± 0.16
3.194 ± 0.222
8.053 ± 0.22
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.1 ± 0.11
3.038 ± 0.175
6.467 ± 0.245
3.619 ± 0.176
7.299 ± 0.318
5.283 ± 0.213
6.199 ± 0.24
6.936 ± 0.244
2.066 ± 0.139
2.329 ± 0.14
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here