Amino acid dipepetide frequency for Treponema sp. JC4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.576AlaAla: 7.576 ± 0.121	1.113AlaCys: 1.113 ± 0.041	4.839AlaAsp: 4.839 ± 0.086	5.852AlaGlu: 5.852 ± 0.101	3.605AlaPhe: 3.605 ± 0.061	6.061AlaGly: 6.061 ± 0.105	1.043AlaHis: 1.043 ± 0.041	5.024AlaIle: 5.024 ± 0.086	5.548AlaLys: 5.548 ± 0.091	7.226AlaLeu: 7.226 ± 0.097	1.875AlaMet: 1.875 ± 0.046	3.131AlaAsn: 3.131 ± 0.069	2.168AlaPro: 2.168 ± 0.062	2.503AlaGln: 2.503 ± 0.055	2.888AlaArg: 2.888 ± 0.066	4.848AlaSer: 4.848 ± 0.087	3.227AlaThr: 3.227 ± 0.07	5.526AlaVal: 5.526 ± 0.084	0.604AlaTrp: 0.604 ± 0.027	2.539AlaTyr: 2.539 ± 0.053	0.0AlaXaa: 0.0 ± 0.0
Cys	1.095CysAla: 1.095 ± 0.039	0.238CysCys: 0.238 ± 0.015	0.861CysAsp: 0.861 ± 0.033	0.808CysGlu: 0.808 ± 0.033	0.639CysPhe: 0.639 ± 0.027	1.32CysGly: 1.32 ± 0.048	0.273CysHis: 0.273 ± 0.021	0.979CysIle: 0.979 ± 0.032	0.936CysLys: 0.936 ± 0.036	1.105CysLeu: 1.105 ± 0.034	0.32CysMet: 0.32 ± 0.023	0.571CysAsn: 0.571 ± 0.024	0.569CysPro: 0.569 ± 0.028	0.42CysGln: 0.42 ± 0.023	0.626CysArg: 0.626 ± 0.027	0.921CysSer: 0.921 ± 0.035	0.74CysThr: 0.74 ± 0.032	0.867CysVal: 0.867 ± 0.039	0.12CysTrp: 0.12 ± 0.013	0.528CysTyr: 0.528 ± 0.025	0.0CysXaa: 0.0 ± 0.0
Asp	4.048AspAla: 4.048 ± 0.076	0.812AspCys: 0.812 ± 0.036	3.367AspAsp: 3.367 ± 0.088	4.572AspGlu: 4.572 ± 0.098	3.949AspPhe: 3.949 ± 0.072	4.139AspGly: 4.139 ± 0.097	0.673AspHis: 0.673 ± 0.03	4.228AspIle: 4.228 ± 0.064	3.969AspLys: 3.969 ± 0.076	4.783AspLeu: 4.783 ± 0.083	1.435AspMet: 1.435 ± 0.044	2.526AspAsn: 2.526 ± 0.062	1.658AspPro: 1.658 ± 0.046	1.286AspGln: 1.286 ± 0.039	1.879AspArg: 1.879 ± 0.05	3.982AspSer: 3.982 ± 0.094	2.782AspThr: 2.782 ± 0.072	3.491AspVal: 3.491 ± 0.061	0.716AspTrp: 0.716 ± 0.028	2.695AspTyr: 2.695 ± 0.057	0.0AspXaa: 0.0 ± 0.0
Glu	5.188GluAla: 5.188 ± 0.088	0.712GluCys: 0.712 ± 0.031	3.708GluAsp: 3.708 ± 0.084	5.866GluGlu: 5.866 ± 0.128	2.815GluPhe: 2.815 ± 0.054	4.114GluGly: 4.114 ± 0.079	1.113GluHis: 1.113 ± 0.038	5.584GluIle: 5.584 ± 0.08	7.086GluLys: 7.086 ± 0.115	6.286GluLeu: 6.286 ± 0.109	1.742GluMet: 1.742 ± 0.041	4.741GluAsn: 4.741 ± 0.082	1.753GluPro: 1.753 ± 0.058	2.179GluGln: 2.179 ± 0.057	2.79GluArg: 2.79 ± 0.061	3.854GluSer: 3.854 ± 0.077	3.448GluThr: 3.448 ± 0.075	4.074GluVal: 4.074 ± 0.075	0.684GluTrp: 0.684 ± 0.027	2.578GluTyr: 2.578 ± 0.054	0.0GluXaa: 0.0 ± 0.0
Phe	3.824PheAla: 3.824 ± 0.068	0.948PheCys: 0.948 ± 0.036	3.144PheAsp: 3.144 ± 0.066	3.132PheGlu: 3.132 ± 0.061	2.525PhePhe: 2.525 ± 0.058	3.31PheGly: 3.31 ± 0.065	0.665PheHis: 0.665 ± 0.028	3.644PheIle: 3.644 ± 0.081	2.945PheLys: 2.945 ± 0.068	4.045PheLeu: 4.045 ± 0.086	1.224PheMet: 1.224 ± 0.038	2.348PheAsn: 2.348 ± 0.055	1.722PhePro: 1.722 ± 0.051	1.174PheGln: 1.174 ± 0.037	1.662PheArg: 1.662 ± 0.053	4.048PheSer: 4.048 ± 0.077	3.06PheThr: 3.06 ± 0.062	3.09PheVal: 3.09 ± 0.071	0.458PheTrp: 0.458 ± 0.024	2.107PheTyr: 2.107 ± 0.05	0.001PheXaa: 0.001 ± 0.001
Gly	4.817GlyAla: 4.817 ± 0.096	0.988GlyCys: 0.988 ± 0.036	3.373GlyAsp: 3.373 ± 0.064	3.939GlyGlu: 3.939 ± 0.068	3.478GlyPhe: 3.478 ± 0.072	4.67GlyGly: 4.67 ± 0.124	1.026GlyHis: 1.026 ± 0.031	5.379GlyIle: 5.379 ± 0.093	5.641GlyLys: 5.641 ± 0.086	5.865GlyLeu: 5.865 ± 0.099	1.798GlyMet: 1.798 ± 0.058	3.192GlyAsn: 3.192 ± 0.076	1.243GlyPro: 1.243 ± 0.04	1.882GlyGln: 1.882 ± 0.048	2.645GlyArg: 2.645 ± 0.066	4.446GlySer: 4.446 ± 0.086	4.041GlyThr: 4.041 ± 0.086	4.172GlyVal: 4.172 ± 0.073	0.782GlyTrp: 0.782 ± 0.035	2.602GlyTyr: 2.602 ± 0.06	0.0GlyXaa: 0.0 ± 0.0
His	1.024HisAla: 1.024 ± 0.038	0.233HisCys: 0.233 ± 0.016	0.883HisAsp: 0.883 ± 0.032	0.894HisGlu: 0.894 ± 0.034	0.871HisPhe: 0.871 ± 0.033	1.081HisGly: 1.081 ± 0.033	0.344HisHis: 0.344 ± 0.021	1.164HisIle: 1.164 ± 0.036	1.053HisLys: 1.053 ± 0.032	1.367HisLeu: 1.367 ± 0.04	0.307HisMet: 0.307 ± 0.019	0.773HisAsn: 0.773 ± 0.029	0.752HisPro: 0.752 ± 0.03	0.476HisGln: 0.476 ± 0.023	0.577HisArg: 0.577 ± 0.025	0.946HisSer: 0.946 ± 0.033	0.874HisThr: 0.874 ± 0.032	0.89HisVal: 0.89 ± 0.032	0.165HisTrp: 0.165 ± 0.014	0.64HisTyr: 0.64 ± 0.03	0.0HisXaa: 0.0 ± 0.0
Ile	5.646IleAla: 5.646 ± 0.09	1.24IleCys: 1.24 ± 0.043	4.167IleAsp: 4.167 ± 0.074	4.914IleGlu: 4.914 ± 0.082	3.588IlePhe: 3.588 ± 0.081	4.354IleGly: 4.354 ± 0.087	1.087IleHis: 1.087 ± 0.036	5.56IleIle: 5.56 ± 0.104	5.363IleLys: 5.363 ± 0.082	6.777IleLeu: 6.777 ± 0.099	1.62IleMet: 1.62 ± 0.047	3.566IleAsn: 3.566 ± 0.066	3.402IlePro: 3.402 ± 0.067	2.425IleGln: 2.425 ± 0.056	2.952IleArg: 2.952 ± 0.055	5.623IleSer: 5.623 ± 0.078	4.691IleThr: 4.691 ± 0.083	4.376IleVal: 4.376 ± 0.075	0.58IleTrp: 0.58 ± 0.026	2.615IleTyr: 2.615 ± 0.059	0.0IleXaa: 0.0 ± 0.0
Lys	6.095LysAla: 6.095 ± 0.096	0.769LysCys: 0.769 ± 0.032	4.339LysAsp: 4.339 ± 0.085	5.801LysGlu: 5.801 ± 0.094	2.672LysPhe: 2.672 ± 0.059	4.16LysGly: 4.16 ± 0.076	1.004LysHis: 1.004 ± 0.041	6.598LysIle: 6.598 ± 0.093	7.21LysLys: 7.21 ± 0.109	6.39LysLeu: 6.39 ± 0.094	2.119LysMet: 2.119 ± 0.051	5.006LysAsn: 5.006 ± 0.08	2.278LysPro: 2.278 ± 0.051	2.128LysGln: 2.128 ± 0.053	2.665LysArg: 2.665 ± 0.061	4.457LysSer: 4.457 ± 0.07	4.656LysThr: 4.656 ± 0.084	4.4LysVal: 4.4 ± 0.08	0.656LysTrp: 0.656 ± 0.029	2.724LysTyr: 2.724 ± 0.059	0.0LysXaa: 0.0 ± 0.0
Leu	6.865LeuAla: 6.865 ± 0.092	1.566LeuCys: 1.566 ± 0.052	4.794LeuAsp: 4.794 ± 0.082	6.079LeuGlu: 6.079 ± 0.086	4.434LeuPhe: 4.434 ± 0.087	5.58LeuGly: 5.58 ± 0.077	1.489LeuHis: 1.489 ± 0.049	6.104LeuIle: 6.104 ± 0.107	6.729LeuLys: 6.729 ± 0.102	8.21LeuLeu: 8.21 ± 0.137	2.093LeuMet: 2.093 ± 0.049	4.424LeuAsn: 4.424 ± 0.071	3.877LeuPro: 3.877 ± 0.063	2.82LeuGln: 2.82 ± 0.064	3.399LeuArg: 3.399 ± 0.063	7.129LeuSer: 7.129 ± 0.113	5.235LeuThr: 5.235 ± 0.086	5.122LeuVal: 5.122 ± 0.097	0.749LeuTrp: 0.749 ± 0.036	3.324LeuTyr: 3.324 ± 0.082	0.0LeuXaa: 0.0 ± 0.0
Met	2.012MetAla: 2.012 ± 0.051	0.264MetCys: 0.264 ± 0.017	1.296MetAsp: 1.296 ± 0.038	1.722MetGlu: 1.722 ± 0.044	0.926MetPhe: 0.926 ± 0.028	1.503MetGly: 1.503 ± 0.042	0.373MetHis: 0.373 ± 0.023	1.823MetIle: 1.823 ± 0.054	2.267MetLys: 2.267 ± 0.048	2.11MetLeu: 2.11 ± 0.051	0.647MetMet: 0.647 ± 0.03	1.464MetAsn: 1.464 ± 0.036	0.948MetPro: 0.948 ± 0.035	0.858MetGln: 0.858 ± 0.029	0.859MetArg: 0.859 ± 0.028	1.518MetSer: 1.518 ± 0.042	1.48MetThr: 1.48 ± 0.043	1.328MetVal: 1.328 ± 0.038	0.145MetTrp: 0.145 ± 0.013	0.751MetTyr: 0.751 ± 0.027	0.0MetXaa: 0.0 ± 0.0
Asn	3.679AsnAla: 3.679 ± 0.074	0.718AsnCys: 0.718 ± 0.032	2.621AsnAsp: 2.621 ± 0.057	3.156AsnGlu: 3.156 ± 0.066	2.456AsnPhe: 2.456 ± 0.063	3.61AsnGly: 3.61 ± 0.081	0.905AsnHis: 0.905 ± 0.033	3.741AsnIle: 3.741 ± 0.076	3.399AsnLys: 3.399 ± 0.063	4.767AsnLeu: 4.767 ± 0.069	1.286AsnMet: 1.286 ± 0.046	2.343AsnAsn: 2.343 ± 0.059	2.513AsnPro: 2.513 ± 0.056	1.88AsnGln: 1.88 ± 0.053	1.937AsnArg: 1.937 ± 0.058	3.188AsnSer: 3.188 ± 0.069	2.584AsnThr: 2.584 ± 0.07	2.939AsnVal: 2.939 ± 0.056	0.609AsnTrp: 0.609 ± 0.028	2.184AsnTyr: 2.184 ± 0.053	0.0AsnXaa: 0.0 ± 0.0
Pro	3.009ProAla: 3.009 ± 0.071	0.418ProCys: 0.418 ± 0.019	2.451ProAsp: 2.451 ± 0.058	3.273ProGlu: 3.273 ± 0.075	1.743ProPhe: 1.743 ± 0.049	2.07ProGly: 2.07 ± 0.059	0.561ProHis: 0.561 ± 0.027	1.837ProIle: 1.837 ± 0.052	2.042ProLys: 2.042 ± 0.057	3.183ProLeu: 3.183 ± 0.061	0.68ProMet: 0.68 ± 0.027	1.331ProAsn: 1.331 ± 0.042	0.858ProPro: 0.858 ± 0.034	1.185ProGln: 1.185 ± 0.043	1.012ProArg: 1.012 ± 0.032	2.235ProSer: 2.235 ± 0.052	1.465ProThr: 1.465 ± 0.045	3.087ProVal: 3.087 ± 0.075	0.32ProTrp: 0.32 ± 0.019	1.444ProTyr: 1.444 ± 0.04	0.0ProXaa: 0.0 ± 0.0
Gln	2.366GlnAla: 2.366 ± 0.063	0.267GlnCys: 0.267 ± 0.016	1.413GlnAsp: 1.413 ± 0.045	1.954GlnGlu: 1.954 ± 0.051	1.296GlnPhe: 1.296 ± 0.036	1.807GlnGly: 1.807 ± 0.052	0.44GlnHis: 0.44 ± 0.019	2.748GlnIle: 2.748 ± 0.06	2.935GlnLys: 2.935 ± 0.058	2.894GlnLeu: 2.894 ± 0.075	0.918GlnMet: 0.918 ± 0.031	2.18GlnAsn: 2.18 ± 0.052	0.942GlnPro: 0.942 ± 0.031	1.069GlnGln: 1.069 ± 0.043	1.167GlnArg: 1.167 ± 0.036	1.816GlnSer: 1.816 ± 0.047	1.879GlnThr: 1.879 ± 0.055	1.658GlnVal: 1.658 ± 0.043	0.259GlnTrp: 0.259 ± 0.018	1.048GlnTyr: 1.048 ± 0.042	0.0GlnXaa: 0.0 ± 0.0
Arg	2.726ArgAla: 2.726 ± 0.057	0.418ArgCys: 0.418 ± 0.023	2.014ArgAsp: 2.014 ± 0.052	2.65ArgGlu: 2.65 ± 0.064	1.867ArgPhe: 1.867 ± 0.047	2.083ArgGly: 2.083 ± 0.047	0.664ArgHis: 0.664 ± 0.028	3.005ArgIle: 3.005 ± 0.063	2.869ArgLys: 2.869 ± 0.06	3.602ArgLeu: 3.602 ± 0.075	1.039ArgMet: 1.039 ± 0.037	1.964ArgAsn: 1.964 ± 0.047	1.234ArgPro: 1.234 ± 0.04	1.378ArgGln: 1.378 ± 0.041	1.793ArgArg: 1.793 ± 0.051	2.024ArgSer: 2.024 ± 0.043	2.012ArgThr: 2.012 ± 0.054	2.256ArgVal: 2.256 ± 0.05	0.378ArgTrp: 0.378 ± 0.02	1.504ArgTyr: 1.504 ± 0.041	0.0ArgXaa: 0.0 ± 0.0
Ser	5.134SerAla: 5.134 ± 0.076	0.919SerCys: 0.919 ± 0.03	4.39SerAsp: 4.39 ± 0.1	4.734SerGlu: 4.734 ± 0.088	3.651SerPhe: 3.651 ± 0.068	5.224SerGly: 5.224 ± 0.099	0.992SerHis: 0.992 ± 0.037	4.653SerIle: 4.653 ± 0.073	4.306SerLys: 4.306 ± 0.08	6.316SerLeu: 6.316 ± 0.102	1.447SerMet: 1.447 ± 0.043	2.786SerAsn: 2.786 ± 0.074	2.104SerPro: 2.104 ± 0.049	2.265SerGln: 2.265 ± 0.055	2.493SerArg: 2.493 ± 0.052	4.936SerSer: 4.936 ± 0.126	3.28SerThr: 3.28 ± 0.087	4.68SerVal: 4.68 ± 0.083	0.767SerTrp: 0.767 ± 0.036	2.612SerTyr: 2.612 ± 0.073	0.0SerXaa: 0.0 ± 0.0
Thr	4.578ThrAla: 4.578 ± 0.09	0.665ThrCys: 0.665 ± 0.031	3.116ThrAsp: 3.116 ± 0.078	3.678ThrGlu: 3.678 ± 0.067	2.674ThrPhe: 2.674 ± 0.053	4.346ThrGly: 4.346 ± 0.078	0.837ThrHis: 0.837 ± 0.032	4.169ThrIle: 4.169 ± 0.09	3.518ThrLys: 3.518 ± 0.066	4.994ThrLeu: 4.994 ± 0.082	1.149ThrMet: 1.149 ± 0.035	2.355ThrAsn: 2.355 ± 0.061	2.263ThrPro: 2.263 ± 0.055	1.639ThrGln: 1.639 ± 0.048	1.694ThrArg: 1.694 ± 0.04	3.811ThrSer: 3.811 ± 0.092	2.759ThrThr: 2.759 ± 0.073	3.98ThrVal: 3.98 ± 0.074	0.502ThrTrp: 0.502 ± 0.027	2.014ThrTyr: 2.014 ± 0.06	0.0ThrXaa: 0.0 ± 0.0
Val	4.258ValAla: 4.258 ± 0.074	1.005ValCys: 1.005 ± 0.035	3.194ValAsp: 3.194 ± 0.071	4.131ValGlu: 4.131 ± 0.074	3.337ValPhe: 3.337 ± 0.064	3.465ValGly: 3.465 ± 0.068	0.955ValHis: 0.955 ± 0.032	4.944ValIle: 4.944 ± 0.083	4.675ValLys: 4.675 ± 0.07	5.972ValLeu: 5.972 ± 0.105	1.555ValMet: 1.555 ± 0.044	3.031ValAsn: 3.031 ± 0.055	2.339ValPro: 2.339 ± 0.054	1.968ValGln: 1.968 ± 0.049	2.602ValArg: 2.602 ± 0.057	4.598ValSer: 4.598 ± 0.097	3.752ValThr: 3.752 ± 0.073	3.923ValVal: 3.923 ± 0.079	0.533ValTrp: 0.533 ± 0.023	2.318ValTyr: 2.318 ± 0.058	0.0ValXaa: 0.0 ± 0.0
Trp	0.61TrpAla: 0.61 ± 0.028	0.133TrpCys: 0.133 ± 0.012	0.518TrpAsp: 0.518 ± 0.028	0.639TrpGlu: 0.639 ± 0.028	0.469TrpPhe: 0.469 ± 0.025	0.642TrpGly: 0.642 ± 0.032	0.228TrpHis: 0.228 ± 0.015	0.678TrpIle: 0.678 ± 0.03	0.794TrpLys: 0.794 ± 0.029	0.85TrpLeu: 0.85 ± 0.034	0.219TrpMet: 0.219 ± 0.017	0.7TrpAsn: 0.7 ± 0.029	0.244TrpPro: 0.244 ± 0.018	0.373TrpGln: 0.373 ± 0.023	0.35TrpArg: 0.35 ± 0.021	0.582TrpSer: 0.582 ± 0.024	0.529TrpThr: 0.529 ± 0.025	0.44TrpVal: 0.44 ± 0.022	0.142TrpTrp: 0.142 ± 0.015	0.449TrpTyr: 0.449 ± 0.025	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.645TyrAla: 2.645 ± 0.058	0.508TyrCys: 0.508 ± 0.027	2.502TyrAsp: 2.502 ± 0.071	2.518TyrGlu: 2.518 ± 0.056	2.082TyrPhe: 2.082 ± 0.059	2.44TyrGly: 2.44 ± 0.068	0.644TyrHis: 0.644 ± 0.028	2.684TyrIle: 2.684 ± 0.057	2.841TyrLys: 2.841 ± 0.065	3.279TyrLeu: 3.279 ± 0.065	0.823TyrMet: 0.823 ± 0.033	2.082TyrAsn: 2.082 ± 0.057	1.336TyrPro: 1.336 ± 0.044	1.148TyrGln: 1.148 ± 0.037	1.492TyrArg: 1.492 ± 0.044	2.729TyrSer: 2.729 ± 0.074	2.246TyrThr: 2.246 ± 0.054	2.258TyrVal: 2.258 ± 0.061	0.418TyrTrp: 0.418 ± 0.025	1.701TyrTyr: 1.701 ± 0.062	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.001XaaPhe: 0.001 ± 0.001	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2570 proteins (882495 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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