Amino acid dipepetide frequency for Paenibacillus sp. VKM B-2647

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.962AlaAla: 11.962 ± 0.147
0.797AlaCys: 0.797 ± 0.022
4.893AlaAsp: 4.893 ± 0.062
6.213AlaGlu: 6.213 ± 0.071
3.654AlaPhe: 3.654 ± 0.06
7.997AlaGly: 7.997 ± 0.092
1.623AlaHis: 1.623 ± 0.035
5.573AlaIle: 5.573 ± 0.066
4.843AlaLys: 4.843 ± 0.059
9.536AlaLeu: 9.536 ± 0.104
2.579AlaMet: 2.579 ± 0.039
2.698AlaAsn: 2.698 ± 0.044
3.325AlaPro: 3.325 ± 0.062
3.067AlaGln: 3.067 ± 0.053
4.439AlaArg: 4.439 ± 0.051
5.231AlaSer: 5.231 ± 0.059
3.834AlaThr: 3.834 ± 0.066
7.459AlaVal: 7.459 ± 0.081
1.09AlaTrp: 1.09 ± 0.029
2.831AlaTyr: 2.831 ± 0.049
0.0AlaXaa: 0.0 ± 0.0
Cys
0.652CysAla: 0.652 ± 0.02
0.145CysCys: 0.145 ± 0.01
0.397CysAsp: 0.397 ± 0.018
0.489CysGlu: 0.489 ± 0.02
0.353CysPhe: 0.353 ± 0.014
0.863CysGly: 0.863 ± 0.026
0.211CysHis: 0.211 ± 0.014
0.501CysIle: 0.501 ± 0.018
0.335CysLys: 0.335 ± 0.016
0.811CysLeu: 0.811 ± 0.025
0.226CysMet: 0.226 ± 0.012
0.264CysAsn: 0.264 ± 0.012
0.381CysPro: 0.381 ± 0.017
0.201CysGln: 0.201 ± 0.012
0.597CysArg: 0.597 ± 0.02
0.618CysSer: 0.618 ± 0.022
0.433CysThr: 0.433 ± 0.016
0.511CysVal: 0.511 ± 0.02
0.104CysTrp: 0.104 ± 0.009
0.27CysTyr: 0.27 ± 0.014
0.0CysXaa: 0.0 ± 0.0
Asp
4.468AspAla: 4.468 ± 0.064
0.41AspCys: 0.41 ± 0.017
2.582AspAsp: 2.582 ± 0.046
3.557AspGlu: 3.557 ± 0.05
2.146AspPhe: 2.146 ± 0.039
4.116AspGly: 4.116 ± 0.064
1.002AspHis: 1.002 ± 0.026
3.493AspIle: 3.493 ± 0.045
2.76AspLys: 2.76 ± 0.048
4.545AspLeu: 4.545 ± 0.053
1.326AspMet: 1.326 ± 0.032
1.709AspAsn: 1.709 ± 0.037
2.542AspPro: 2.542 ± 0.051
1.525AspGln: 1.525 ± 0.031
2.956AspArg: 2.956 ± 0.05
2.46AspSer: 2.46 ± 0.045
2.306AspThr: 2.306 ± 0.039
3.808AspVal: 3.808 ± 0.052
0.819AspTrp: 0.819 ± 0.03
2.008AspTyr: 2.008 ± 0.038
0.0AspXaa: 0.0 ± 0.0
Glu
6.395GluAla: 6.395 ± 0.065
0.396GluCys: 0.396 ± 0.017
2.582GluAsp: 2.582 ± 0.043
4.443GluGlu: 4.443 ± 0.071
2.093GluPhe: 2.093 ± 0.042
3.833GluGly: 3.833 ± 0.053
1.526GluHis: 1.526 ± 0.033
3.599GluIle: 3.599 ± 0.059
3.414GluLys: 3.414 ± 0.056
7.318GluLeu: 7.318 ± 0.086
1.742GluMet: 1.742 ± 0.037
1.997GluAsn: 1.997 ± 0.042
2.473GluPro: 2.473 ± 0.042
3.398GluGln: 3.398 ± 0.056
4.628GluArg: 4.628 ± 0.066
3.013GluSer: 3.013 ± 0.045
3.349GluThr: 3.349 ± 0.051
3.892GluVal: 3.892 ± 0.053
0.919GluTrp: 0.919 ± 0.027
1.824GluTyr: 1.824 ± 0.04
0.0GluXaa: 0.0 ± 0.0
Phe
3.848PheAla: 3.848 ± 0.056
0.374PheCys: 0.374 ± 0.018
2.272PheAsp: 2.272 ± 0.039
2.302PheGlu: 2.302 ± 0.04
1.871PhePhe: 1.871 ± 0.042
3.535PheGly: 3.535 ± 0.049
0.947PheHis: 0.947 ± 0.024
2.642PheIle: 2.642 ± 0.046
1.757PheLys: 1.757 ± 0.037
4.019PheLeu: 4.019 ± 0.049
1.126PheMet: 1.126 ± 0.028
1.412PheAsn: 1.412 ± 0.032
1.746PhePro: 1.746 ± 0.034
1.38PheGln: 1.38 ± 0.031
2.389PheArg: 2.389 ± 0.045
2.593PheSer: 2.593 ± 0.047
2.331PheThr: 2.331 ± 0.042
3.291PheVal: 3.291 ± 0.046
0.581PheTrp: 0.581 ± 0.023
1.501PheTyr: 1.501 ± 0.038
0.0PheXaa: 0.0 ± 0.0
Gly
6.553GlyAla: 6.553 ± 0.075
0.855GlyCys: 0.855 ± 0.026
3.586GlyAsp: 3.586 ± 0.055
4.345GlyGlu: 4.345 ± 0.051
3.332GlyPhe: 3.332 ± 0.053
5.955GlyGly: 5.955 ± 0.09
1.555GlyHis: 1.555 ± 0.032
5.568GlyIle: 5.568 ± 0.066
4.629GlyLys: 4.629 ± 0.069
7.448GlyLeu: 7.448 ± 0.086
2.345GlyMet: 2.345 ± 0.041
2.495GlyAsn: 2.495 ± 0.047
2.206GlyPro: 2.206 ± 0.037
2.62GlyGln: 2.62 ± 0.04
4.036GlyArg: 4.036 ± 0.058
4.523GlySer: 4.523 ± 0.071
4.352GlyThr: 4.352 ± 0.063
5.414GlyVal: 5.414 ± 0.066
1.205GlyTrp: 1.205 ± 0.034
2.874GlyTyr: 2.874 ± 0.042
0.0GlyXaa: 0.0 ± 0.0
His
1.711HisAla: 1.711 ± 0.037
0.215HisCys: 0.215 ± 0.013
1.068HisAsp: 1.068 ± 0.03
1.268HisGlu: 1.268 ± 0.034
1.102HisPhe: 1.102 ± 0.028
1.675HisGly: 1.675 ± 0.035
0.613HisHis: 0.613 ± 0.024
1.403HisIle: 1.403 ± 0.03
0.839HisLys: 0.839 ± 0.024
2.073HisLeu: 2.073 ± 0.039
0.605HisMet: 0.605 ± 0.018
0.673HisAsn: 0.673 ± 0.019
1.248HisPro: 1.248 ± 0.029
0.655HisGln: 0.655 ± 0.022
1.264HisArg: 1.264 ± 0.03
1.095HisSer: 1.095 ± 0.026
1.016HisThr: 1.016 ± 0.025
1.504HisVal: 1.504 ± 0.034
0.312HisTrp: 0.312 ± 0.017
0.81HisTyr: 0.81 ± 0.028
0.0HisXaa: 0.0 ± 0.0
Ile
6.365IleAla: 6.365 ± 0.071
0.608IleCys: 0.608 ± 0.018
3.653IleAsp: 3.653 ± 0.056
3.845IleGlu: 3.845 ± 0.061
2.291IlePhe: 2.291 ± 0.045
5.357IleGly: 5.357 ± 0.064
1.288IleHis: 1.288 ± 0.027
3.456IleIle: 3.456 ± 0.055
2.593IleLys: 2.593 ± 0.051
5.024IleLeu: 5.024 ± 0.066
1.564IleMet: 1.564 ± 0.031
2.069IleAsn: 2.069 ± 0.043
3.0IlePro: 3.0 ± 0.043
2.068IleGln: 2.068 ± 0.039
4.106IleArg: 4.106 ± 0.059
3.77IleSer: 3.77 ± 0.058
3.127IleThr: 3.127 ± 0.046
5.632IleVal: 5.632 ± 0.068
0.684IleTrp: 0.684 ± 0.023
1.91IleTyr: 1.91 ± 0.038
0.0IleXaa: 0.0 ± 0.0
Lys
4.459LysAla: 4.459 ± 0.068
0.277LysCys: 0.277 ± 0.014
2.613LysAsp: 2.613 ± 0.05
3.812LysGlu: 3.812 ± 0.061
1.605LysPhe: 1.605 ± 0.034
3.325LysGly: 3.325 ± 0.044
1.114LysHis: 1.114 ± 0.028
2.871LysIle: 2.871 ± 0.052
3.008LysLys: 3.008 ± 0.057
5.521LysLeu: 5.521 ± 0.075
1.549LysMet: 1.549 ± 0.033
1.884LysAsn: 1.884 ± 0.034
2.449LysPro: 2.449 ± 0.043
2.507LysGln: 2.507 ± 0.042
3.015LysArg: 3.015 ± 0.043
2.778LysSer: 2.778 ± 0.047
2.759LysThr: 2.759 ± 0.042
3.374LysVal: 3.374 ± 0.051
0.715LysTrp: 0.715 ± 0.019
1.728LysTyr: 1.728 ± 0.034
0.0LysXaa: 0.0 ± 0.0
Leu
9.702LeuAla: 9.702 ± 0.096
0.862LeuCys: 0.862 ± 0.028
4.956LeuAsp: 4.956 ± 0.067
5.995LeuGlu: 5.995 ± 0.078
4.699LeuPhe: 4.699 ± 0.065
7.11LeuGly: 7.11 ± 0.08
2.28LeuHis: 2.28 ± 0.041
5.934LeuIle: 5.934 ± 0.08
5.124LeuLys: 5.124 ± 0.062
11.545LeuLeu: 11.545 ± 0.127
2.58LeuMet: 2.58 ± 0.041
3.477LeuAsn: 3.477 ± 0.058
4.83LeuPro: 4.83 ± 0.061
4.366LeuGln: 4.366 ± 0.066
5.803LeuArg: 5.803 ± 0.068
6.593LeuSer: 6.593 ± 0.066
5.483LeuThr: 5.483 ± 0.059
6.632LeuVal: 6.632 ± 0.083
1.155LeuTrp: 1.155 ± 0.03
3.209LeuTyr: 3.209 ± 0.055
0.0LeuXaa: 0.0 ± 0.0
Met
2.536MetAla: 2.536 ± 0.039
0.172MetCys: 0.172 ± 0.011
1.375MetAsp: 1.375 ± 0.03
1.711MetGlu: 1.711 ± 0.038
1.079MetPhe: 1.079 ± 0.029
1.678MetGly: 1.678 ± 0.037
0.484MetHis: 0.484 ± 0.019
1.791MetIle: 1.791 ± 0.036
1.903MetLys: 1.903 ± 0.033
2.987MetLeu: 2.987 ± 0.047
0.89MetMet: 0.89 ± 0.026
1.395MetAsn: 1.395 ± 0.03
1.3MetPro: 1.3 ± 0.03
1.078MetGln: 1.078 ± 0.028
1.551MetArg: 1.551 ± 0.029
1.664MetSer: 1.664 ± 0.037
1.71MetThr: 1.71 ± 0.032
1.631MetVal: 1.631 ± 0.036
0.252MetTrp: 0.252 ± 0.011
0.764MetTyr: 0.764 ± 0.022
0.0MetXaa: 0.0 ± 0.0
Asn
2.902AsnAla: 2.902 ± 0.053
0.254AsnCys: 0.254 ± 0.014
1.74AsnAsp: 1.74 ± 0.039
2.193AsnGlu: 2.193 ± 0.046
1.282AsnPhe: 1.282 ± 0.033
2.943AsnGly: 2.943 ± 0.049
0.698AsnHis: 0.698 ± 0.023
2.133AsnIle: 2.133 ± 0.042
1.811AsnLys: 1.811 ± 0.043
3.043AsnLeu: 3.043 ± 0.049
1.025AsnMet: 1.025 ± 0.027
1.353AsnAsn: 1.353 ± 0.034
1.924AsnPro: 1.924 ± 0.037
1.174AsnGln: 1.174 ± 0.03
2.102AsnArg: 2.102 ± 0.037
1.801AsnSer: 1.801 ± 0.037
1.631AsnThr: 1.631 ± 0.041
2.681AsnVal: 2.681 ± 0.047
0.527AsnTrp: 0.527 ± 0.016
1.274AsnTyr: 1.274 ± 0.031
0.0AsnXaa: 0.0 ± 0.0
Pro
4.185ProAla: 4.185 ± 0.053
0.263ProCys: 0.263 ± 0.014
2.82ProAsp: 2.82 ± 0.049
3.305ProGlu: 3.305 ± 0.054
2.009ProPhe: 2.009 ± 0.035
3.341ProGly: 3.341 ± 0.051
1.039ProHis: 1.039 ± 0.028
2.476ProIle: 2.476 ± 0.039
1.96ProLys: 1.96 ± 0.035
4.374ProLeu: 4.374 ± 0.061
1.062ProMet: 1.062 ± 0.03
1.661ProAsn: 1.661 ± 0.034
1.609ProPro: 1.609 ± 0.037
1.622ProGln: 1.622 ± 0.037
1.677ProArg: 1.677 ± 0.035
2.513ProSer: 2.513 ± 0.048
1.871ProThr: 1.871 ± 0.037
3.479ProVal: 3.479 ± 0.053
0.607ProTrp: 0.607 ± 0.024
1.591ProTyr: 1.591 ± 0.035
0.0ProXaa: 0.0 ± 0.0
Gln
3.656GlnAla: 3.656 ± 0.057
0.239GlnCys: 0.239 ± 0.012
1.57GlnAsp: 1.57 ± 0.029
2.28GlnGlu: 2.28 ± 0.043
1.56GlnPhe: 1.56 ± 0.034
2.458GlnGly: 2.458 ± 0.036
0.806GlnHis: 0.806 ± 0.024
2.378GlnIle: 2.378 ± 0.04
1.854GlnLys: 1.854 ± 0.04
4.199GlnLeu: 4.199 ± 0.062
1.119GlnMet: 1.119 ± 0.03
1.277GlnAsn: 1.277 ± 0.031
1.6GlnPro: 1.6 ± 0.033
1.895GlnGln: 1.895 ± 0.047
2.152GlnArg: 2.152 ± 0.04
2.109GlnSer: 2.109 ± 0.041
2.104GlnThr: 2.104 ± 0.042
2.366GlnVal: 2.366 ± 0.042
0.476GlnTrp: 0.476 ± 0.021
1.279GlnTyr: 1.279 ± 0.03
0.0GlnXaa: 0.0 ± 0.0
Arg
4.099ArgAla: 4.099 ± 0.057
0.492ArgCys: 0.492 ± 0.021
2.818ArgAsp: 2.818 ± 0.054
4.075ArgGlu: 4.075 ± 0.062
2.514ArgPhe: 2.514 ± 0.042
3.542ArgGly: 3.542 ± 0.052
1.297ArgHis: 1.297 ± 0.032
4.002ArgIle: 4.002 ± 0.05
3.243ArgLys: 3.243 ± 0.049
6.213ArgLeu: 6.213 ± 0.066
1.822ArgMet: 1.822 ± 0.037
1.966ArgAsn: 1.966 ± 0.039
2.17ArgPro: 2.17 ± 0.038
2.4ArgGln: 2.4 ± 0.045
3.718ArgArg: 3.718 ± 0.06
3.42ArgSer: 3.42 ± 0.057
3.005ArgThr: 3.005 ± 0.047
3.455ArgVal: 3.455 ± 0.051
0.819ArgTrp: 0.819 ± 0.024
2.004ArgTyr: 2.004 ± 0.042
0.0ArgXaa: 0.0 ± 0.0
Ser
5.187SerAla: 5.187 ± 0.06
0.47SerCys: 0.47 ± 0.018
2.875SerAsp: 2.875 ± 0.044
3.271SerGlu: 3.271 ± 0.05
2.816SerPhe: 2.816 ± 0.046
5.462SerGly: 5.462 ± 0.073
1.121SerHis: 1.121 ± 0.028
3.718SerIle: 3.718 ± 0.062
2.811SerLys: 2.811 ± 0.05
6.105SerLeu: 6.105 ± 0.069
1.624SerMet: 1.624 ± 0.031
1.81SerAsn: 1.81 ± 0.039
2.515SerPro: 2.515 ± 0.046
1.764SerGln: 1.764 ± 0.038
3.232SerArg: 3.232 ± 0.048
3.637SerSer: 3.637 ± 0.058
2.567SerThr: 2.567 ± 0.05
4.33SerVal: 4.33 ± 0.055
0.779SerTrp: 0.779 ± 0.026
2.056SerTyr: 2.056 ± 0.04
0.0SerXaa: 0.0 ± 0.0
Thr
4.839ThrAla: 4.839 ± 0.062
0.363ThrCys: 0.363 ± 0.017
2.749ThrAsp: 2.749 ± 0.046
2.793ThrGlu: 2.793 ± 0.04
2.303ThrPhe: 2.303 ± 0.041
4.363ThrGly: 4.363 ± 0.059
0.93ThrHis: 0.93 ± 0.026
3.405ThrIle: 3.405 ± 0.058
2.369ThrLys: 2.369 ± 0.041
5.206ThrLeu: 5.206 ± 0.06
1.382ThrMet: 1.382 ± 0.029
1.777ThrAsn: 1.777 ± 0.036
2.545ThrPro: 2.545 ± 0.044
1.425ThrGln: 1.425 ± 0.031
2.212ThrArg: 2.212 ± 0.038
2.874ThrSer: 2.874 ± 0.05
2.484ThrThr: 2.484 ± 0.056
4.608ThrVal: 4.608 ± 0.056
0.637ThrTrp: 0.637 ± 0.021
1.827ThrTyr: 1.827 ± 0.036
0.0ThrXaa: 0.0 ± 0.0
Val
6.25ValAla: 6.25 ± 0.073
0.743ValCys: 0.743 ± 0.022
3.515ValAsp: 3.515 ± 0.054
4.089ValGlu: 4.089 ± 0.06
3.058ValPhe: 3.058 ± 0.052
4.763ValGly: 4.763 ± 0.059
1.558ValHis: 1.558 ± 0.035
4.582ValIle: 4.582 ± 0.057
3.864ValLys: 3.864 ± 0.057
7.522ValLeu: 7.522 ± 0.073
2.031ValMet: 2.031 ± 0.04
2.623ValAsn: 2.623 ± 0.05
3.556ValPro: 3.556 ± 0.059
2.685ValGln: 2.685 ± 0.042
4.202ValArg: 4.202 ± 0.062
4.739ValSer: 4.739 ± 0.053
4.294ValThr: 4.294 ± 0.062
5.344ValVal: 5.344 ± 0.058
0.96ValTrp: 0.96 ± 0.029
2.441ValTyr: 2.441 ± 0.037
0.0ValXaa: 0.0 ± 0.0
Trp
0.93TrpAla: 0.93 ± 0.031
0.108TrpCys: 0.108 ± 0.009
0.651TrpAsp: 0.651 ± 0.021
0.704TrpGlu: 0.704 ± 0.022
0.585TrpPhe: 0.585 ± 0.02
0.861TrpGly: 0.861 ± 0.026
0.337TrpHis: 0.337 ± 0.017
0.9TrpIle: 0.9 ± 0.025
0.71TrpLys: 0.71 ± 0.021
1.593TrpLeu: 1.593 ± 0.041
0.476TrpMet: 0.476 ± 0.018
0.691TrpAsn: 0.691 ± 0.023
0.448TrpPro: 0.448 ± 0.019
0.512TrpGln: 0.512 ± 0.022
0.745TrpArg: 0.745 ± 0.025
0.81TrpSer: 0.81 ± 0.024
0.744TrpThr: 0.744 ± 0.023
0.88TrpVal: 0.88 ± 0.027
0.19TrpTrp: 0.19 ± 0.012
0.42TrpTyr: 0.42 ± 0.02
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.943TyrAla: 2.943 ± 0.057
0.321TyrCys: 0.321 ± 0.016
1.822TyrAsp: 1.822 ± 0.037
2.145TyrGlu: 2.145 ± 0.038
1.565TyrPhe: 1.565 ± 0.035
2.719TyrGly: 2.719 ± 0.048
0.72TyrHis: 0.72 ± 0.022
1.985TyrIle: 1.985 ± 0.039
1.532TyrLys: 1.532 ± 0.034
3.256TyrLeu: 3.256 ± 0.057
0.875TyrMet: 0.875 ± 0.02
1.259TyrAsn: 1.259 ± 0.033
1.6TyrPro: 1.6 ± 0.032
1.035TyrGln: 1.035 ± 0.028
2.251TyrArg: 2.251 ± 0.041
1.91TyrSer: 1.91 ± 0.04
1.689TyrThr: 1.689 ± 0.04
2.531TyrVal: 2.531 ± 0.04
0.462TyrTrp: 0.462 ± 0.018
1.213TyrTyr: 1.213 ± 0.033
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5046 proteins (1449787 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski