Merops nubicus (Northern carmine bee-eater)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida; Sauria; Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7657 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A091R9Y6|A0A091R9Y6_MERNU Uncharacterized protein (Fragment) OS=Merops nubicus OX=57421 GN=N331_06273 PE=4 SV=1
DDD2 pKa = 5.37DDD4 pKa = 3.8VAFCVLAADDD14 pKa = 4.74EE15 pKa = 4.51EE16 pKa = 4.83DD17 pKa = 3.81EE18 pKa = 5.55DDD20 pKa = 3.34ALQIHHH26 pKa = 6.13TLIQAFCCEEE36 pKa = 3.78DDD38 pKa = 2.96DDD40 pKa = 3.95VRR42 pKa = 11.84VNDDD46 pKa = 3.39GKKK49 pKa = 9.94AAIVGPSEEE58 pKa = 4.11EE59 pKa = 4.26GEEE62 pKa = 4.11RR63 pKa = 11.84DDD65 pKa = 3.68HHH67 pKa = 6.98ILITV

Molecular weight: 7.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A091RX38|A0A091RX38_MERNU C-terminal peptide (Fragment) OS=Merops nubicus OX=57421 GN=N331_12150 PE=3 SV=1
TT1 pKa = 6.56QGRR4 pKa = 11.84MSTQRR9 pKa = 11.84RR10 pKa = 11.84VSTQGRR16 pKa = 11.84MSTQGRR22 pKa = 11.84MSTQGRR28 pKa = 11.84MSTQRR33 pKa = 11.84RR34 pKa = 11.84VSTQGRR40 pKa = 11.84MSTQGRR46 pKa = 11.84MSTQGRR52 pKa = 11.84MSTQRR57 pKa = 11.84RR58 pKa = 11.84VSTQGRR64 pKa = 11.84MSTQGRR70 pKa = 11.84MSTQGRR76 pKa = 11.84MSTQRR81 pKa = 11.84RR82 pKa = 11.84VSTQGRR88 pKa = 11.84MSTQGRR94 pKa = 11.84MSTT97 pKa = 3.59

Molecular weight: 10.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7657

0

7657

3228921

33

5554

421.7

47.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.398 ± 0.023

2.241 ± 0.024

5.025 ± 0.02

7.208 ± 0.04

3.932 ± 0.02

5.85 ± 0.032

2.552 ± 0.013

4.979 ± 0.022

6.434 ± 0.031

9.822 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.239 ± 0.012

4.107 ± 0.019

5.253 ± 0.033

4.672 ± 0.029

5.167 ± 0.023

8.179 ± 0.041

5.396 ± 0.02

6.338 ± 0.027

1.193 ± 0.011

3.012 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski