Fusarium poae
Average proteome isoelectric point is 6.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 14048 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B8AFE5|A0A1B8AFE5_FUSPO Importin N-terminal domain-containing protein OS=Fusarium poae OX=36050 GN=FPOA_10887 PE=4 SV=1
MM1 pKa = 7.91 RR2 pKa = 11.84 ISASLGVLVASGLLGHH18 pKa = 6.35 SASQATDD25 pKa = 3.36 YY26 pKa = 11.58 SNTNGSPFGNQPDD39 pKa = 4.04 GSGNAIPGNNGGVSNTLPYY58 pKa = 9.96 PSNAVGSIPGSLPSGVGPVGPVFPGDD84 pKa = 3.79 GSNGLPPTGPVGGPEE99 pKa = 4.14 NLPGSASPGGGSNGPYY115 pKa = 9.83 PNGPGGIPGNLPGSGSPADD134 pKa = 4.54 DD135 pKa = 4.66 ANGLPPNGPTGGPGNVPGSIPGSSSSNGNGNFPGAGSVIIPGSFPTSIPQNVPSSPDD192 pKa = 3.3 SPLSGASDD200 pKa = 3.32 IPTCPFRR207 pKa = 11.84 STKK210 pKa = 9.61 TVVVTIYY217 pKa = 8.73 PTDD220 pKa = 3.62 TEE222 pKa = 4.56 SEE224 pKa = 4.58 SGFHH228 pKa = 6.49 WPDD231 pKa = 3.72 DD232 pKa = 4.07 SDD234 pKa = 4.57 SDD236 pKa = 4.0 GSYY239 pKa = 10.17 TPTPMPTKK247 pKa = 10.2 PPGITSLSPNSGVPIFTTLTLDD269 pKa = 2.37 IWGPVGSPSNSGSGSNGDD287 pKa = 3.32 TSTSNGNQPSDD298 pKa = 2.99 GSTPEE303 pKa = 4.85 DD304 pKa = 3.45 GSGNNGGNDD313 pKa = 3.21 SHH315 pKa = 7.39 GGNGPDD321 pKa = 4.35 SNGGDD326 pKa = 3.88 DD327 pKa = 3.84 SHH329 pKa = 7.83 NSSDD333 pKa = 4.23 SPFFPPTPGPNGAPQYY349 pKa = 10.86 SDD351 pKa = 3.3 STSEE355 pKa = 4.1 SLSVGNPGASGINTLAPTPLQTPEE379 pKa = 4.4 FPNGGIPGTQPQFPSAGPAQEE400 pKa = 4.7 ASGSLPVTTQAGAEE414 pKa = 4.39 GQGEE418 pKa = 4.27 TSPWFTNVPEE428 pKa = 4.72 GNSAGQPTSPYY439 pKa = 9.19 ITITGQDD446 pKa = 3.76 GLPTVISSDD455 pKa = 3.69 GNVVSGDD462 pKa = 3.76 AQPPAVPGSPQNGNSPEE479 pKa = 4.16 LPSGVSSGFPIPPPIFTGIPGSQNPSNSPAGSLPGSGPDD518 pKa = 4.34 AGVTTCATFTITGPNGLPTVVDD540 pKa = 4.05 STWVVPLSTPSFEE553 pKa = 5.46 ASSQLSATFDD563 pKa = 3.5 PQAPLSSISSQLGLPTIPASGSPEE587 pKa = 4.32 DD588 pKa = 4.12 GSGGSAAIASTSFTMIGADD607 pKa = 3.58 GSPTVVYY614 pKa = 7.28 TTWNIPAATISTGDD628 pKa = 3.7 PGDD631 pKa = 3.92 ASTAASSGGFMTITGLPPFGSSGLGGQITTVPGPDD666 pKa = 3.51 DD667 pKa = 3.38 VAQITTCTSYY677 pKa = 10.58 TVIGADD683 pKa = 3.72 GLPTVIDD690 pKa = 3.71 STFVIPGPVNTDD702 pKa = 2.53 ITVGGDD708 pKa = 3.25 PEE710 pKa = 4.64 LPSGASDD717 pKa = 3.72 ALPNGVTSPATFQVTNTPEE736 pKa = 3.94 IPPFNPTAATGSGGFGNNGITTCTSYY762 pKa = 11.27 TMIGSDD768 pKa = 3.94 GLPTVVDD775 pKa = 4.1 TTWVIPGSANTQSEE789 pKa = 4.72 LPGNPSFVSDD799 pKa = 4.17 SLPTGLPSGIPGPWASSAGIPSDD822 pKa = 3.57 GDD824 pKa = 3.24 QGNVPGATTCITYY837 pKa = 7.52 TTIGQDD843 pKa = 3.73 GLPTIVDD850 pKa = 3.6 TTFVVPVATATPSGTGLILPSTNGDD875 pKa = 3.51 GQTGLSPNPTGAYY888 pKa = 5.9 TTTTTAAILGSDD900 pKa = 4.02 GNATPTVQTIVFSDD914 pKa = 3.83 SSALGVSTSAPQGATSGIDD933 pKa = 3.22 PSGISGPVFTSRR945 pKa = 11.84 DD946 pKa = 3.66 LNSPFTDD953 pKa = 3.45 TPSLNGYY960 pKa = 10.53 DD961 pKa = 3.44 NGIPGASMSAILTDD975 pKa = 4.24 GSAAGEE981 pKa = 4.44 GTSVTGTTTGTLTWTVTSITNPPGAPVFSNGASQPPFVPDD1021 pKa = 4.39 PSNGAPGGPAQPAYY1035 pKa = 10.46 GSLATEE1041 pKa = 4.07 STLWPLSAVQTSTWTNVIKK1060 pKa = 10.92 AEE1062 pKa = 4.24 TTSYY1066 pKa = 9.43 TFNYY1070 pKa = 9.59 PLTTLATVNVPMRR1083 pKa = 11.84 RR1084 pKa = 11.84 LARR1087 pKa = 11.84 RR1088 pKa = 11.84 QSMTAWSNSTTSLSSATSASTVTTSEE1114 pKa = 3.86 ASSPTVCPSGGSIGNTTIDD1133 pKa = 3.95 FDD1135 pKa = 4.04 NSKK1138 pKa = 10.22 PGPLFNPVEE1147 pKa = 4.53 NIWFSGGFLIAPPTSQQSQPYY1168 pKa = 8.41 IPSSGGQLVEE1178 pKa = 4.92 FVPPALSNTTTTISGDD1194 pKa = 3.28 VAQIGMGPHH1203 pKa = 6.48 AASPCFRR1210 pKa = 11.84 FDD1212 pKa = 3.8 FFGANLGCDD1221 pKa = 3.22 ARR1223 pKa = 11.84 GDD1225 pKa = 4.04 EE1226 pKa = 4.75 KK1227 pKa = 10.66 WCQFDD1232 pKa = 3.21 ISAYY1236 pKa = 9.13 RR1237 pKa = 11.84 WNEE1240 pKa = 3.59 TSSTEE1245 pKa = 4.22 EE1246 pKa = 3.76 SIAWSEE1252 pKa = 4.39 TKK1254 pKa = 9.88 QVPACSKK1261 pKa = 9.97 FSEE1264 pKa = 4.56 GGYY1267 pKa = 10.31 EE1268 pKa = 4.07 LTRR1271 pKa = 11.84 VDD1273 pKa = 3.82 LDD1275 pKa = 3.85 GYY1277 pKa = 11.04 KK1278 pKa = 10.5 DD1279 pKa = 3.72 LSSVLITLRR1288 pKa = 11.84 VSSDD1292 pKa = 2.96 LRR1294 pKa = 11.84 VWWGDD1299 pKa = 3.28 DD1300 pKa = 3.8 FRR1302 pKa = 11.84 VGWSDD1307 pKa = 3.36 NSCIAATCRR1316 pKa = 11.84 ANAPSQFVKK1325 pKa = 10.72 RR1326 pKa = 11.84 EE1327 pKa = 4.04 TVISALRR1334 pKa = 11.84 QGVYY1338 pKa = 8.97 RR1339 pKa = 11.84 WTPYY1343 pKa = 9.24 EE1344 pKa = 4.38 LKK1346 pKa = 10.64 RR1347 pKa = 11.84 LDD1349 pKa = 5.16 DD1350 pKa = 3.85 SLVWEE1355 pKa = 4.61 SANN1358 pKa = 3.76
Molecular weight: 135.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.693
IPC2_protein 3.656
IPC_protein 3.706
Toseland 3.465
ProMoST 3.872
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.656
Rodwell 3.528
Grimsley 3.376
Solomon 3.706
Lehninger 3.656
Nozaki 3.808
DTASelect 4.088
Thurlkill 3.528
EMBOSS 3.656
Sillero 3.834
Patrickios 0.935
IPC_peptide 3.694
IPC2_peptide 3.808
IPC2.peptide.svr19 3.743
Protein with the highest isoelectric point:
>tr|A0A1B8B8E8|A0A1B8B8E8_FUSPO Uncharacterized protein OS=Fusarium poae OX=36050 GN=FPOA_02941 PE=4 SV=1
MM1 pKa = 7.86 PLTRR5 pKa = 11.84 THH7 pKa = 6.34 RR8 pKa = 11.84 HH9 pKa = 4.3 SAPRR13 pKa = 11.84 RR14 pKa = 11.84 SIFSTRR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 APARR26 pKa = 11.84 SNRR29 pKa = 11.84 HH30 pKa = 4.32 TVTTTTTTTTKK41 pKa = 9.91 PRR43 pKa = 11.84 RR44 pKa = 11.84 GMFGGGSTARR54 pKa = 11.84 RR55 pKa = 11.84 THH57 pKa = 5.96 GAAPVHH63 pKa = 5.2 HH64 pKa = 6.81 HH65 pKa = 5.26 QRR67 pKa = 11.84 KK68 pKa = 8.6 PSMKK72 pKa = 9.93 DD73 pKa = 3.0 KK74 pKa = 11.38 VSGALLKK81 pKa = 10.61 IKK83 pKa = 10.52 GSLTRR88 pKa = 11.84 RR89 pKa = 11.84 PGVKK93 pKa = 9.89 AAGTRR98 pKa = 11.84 RR99 pKa = 11.84 MHH101 pKa = 5.65 GTDD104 pKa = 2.95 GRR106 pKa = 11.84 GARR109 pKa = 11.84 HH110 pKa = 5.73 RR111 pKa = 11.84 RR112 pKa = 11.84 YY113 pKa = 10.37
Molecular weight: 12.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.425
IPC2_protein 11.008
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.13
Rodwell 12.296
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.018
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.101
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
14048
0
14048
7061983
42
7658
502.7
55.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.139 ± 0.02
1.307 ± 0.008
6.003 ± 0.016
6.347 ± 0.022
3.724 ± 0.011
6.605 ± 0.018
2.446 ± 0.01
5.109 ± 0.012
5.146 ± 0.017
8.685 ± 0.023
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.219 ± 0.009
3.839 ± 0.01
5.834 ± 0.021
4.109 ± 0.016
6.079 ± 0.017
8.041 ± 0.023
5.997 ± 0.022
6.005 ± 0.015
1.578 ± 0.008
2.787 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here