Simian torque teno virus 34
Average proteome isoelectric point is 8.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0C5IA74|A0A0C5IA74_9VIRU Capsid protein OS=Simian torque teno virus 34 OX=1629657 PE=3 SV=1
MM1 pKa = 7.32 SFSRR5 pKa = 11.84 PASGEE10 pKa = 3.79 EE11 pKa = 3.68 PRR13 pKa = 11.84 AAEE16 pKa = 4.32 SDD18 pKa = 4.04 PNQPWAGAGGEE29 pKa = 4.27 FTHH32 pKa = 7.37 RR33 pKa = 11.84 SQGAIRR39 pKa = 11.84 AGLAGRR45 pKa = 11.84 NGQVSKK51 pKa = 8.83 THH53 pKa = 6.53 APPQIGHH60 pKa = 6.5 RR61 pKa = 11.84 EE62 pKa = 4.1 DD63 pKa = 3.39 YY64 pKa = 11.02 QEE66 pKa = 4.51 IYY68 pKa = 11.26 DD69 pKa = 4.31 MEE71 pKa = 6.5 ASGQWEE77 pKa = 4.39 PPQQSIAEE85 pKa = 4.54 RR86 pKa = 11.84 EE87 pKa = 4.11 DD88 pKa = 2.84 DD89 pKa = 3.54 WYY91 pKa = 11.42 RR92 pKa = 11.84 SVWWSHH98 pKa = 6.34 LCFCGCGDD106 pKa = 3.76 PSFHH110 pKa = 7.21 FSLLAHH116 pKa = 7.13 RR117 pKa = 11.84 NQGPRR122 pKa = 11.84 GPPGGPPAPPGGPGPRR138 pKa = 11.84 PLGQGPPQNLARR150 pKa = 11.84 FLPLPALPPAPEE162 pKa = 4.11 RR163 pKa = 11.84 QPPRR167 pKa = 11.84 PGDD170 pKa = 3.63 GDD172 pKa = 3.6 GGAAAAVGGPPAEE185 pKa = 4.41 AADD188 pKa = 3.91 AGWRR192 pKa = 11.84 EE193 pKa = 3.67 EE194 pKa = 4.99 DD195 pKa = 3.47 IAEE198 pKa = 4.2 ALAGLEE204 pKa = 4.04 EE205 pKa = 4.4 AEE207 pKa = 4.45 RR208 pKa = 11.84 GG209 pKa = 3.67
Molecular weight: 22.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.919
IPC2_protein 4.927
IPC_protein 4.838
Toseland 4.762
ProMoST 4.965
Dawson 4.813
Bjellqvist 4.952
Wikipedia 4.66
Rodwell 4.736
Grimsley 4.685
Solomon 4.813
Lehninger 4.762
Nozaki 4.927
DTASelect 5.041
Thurlkill 4.762
EMBOSS 4.698
Sillero 5.003
Patrickios 4.05
IPC_peptide 4.825
IPC2_peptide 5.003
IPC2.peptide.svr19 4.929
Protein with the highest isoelectric point:
>tr|A0A0C5IBL0|A0A0C5IBL0_9VIRU ORF3 OS=Simian torque teno virus 34 OX=1629657 PE=4 SV=1
MM1 pKa = 7.38 ARR3 pKa = 11.84 RR4 pKa = 11.84 RR5 pKa = 11.84 YY6 pKa = 9.56 RR7 pKa = 11.84 RR8 pKa = 11.84 GARR11 pKa = 11.84 RR12 pKa = 11.84 TRR14 pKa = 11.84 RR15 pKa = 11.84 GRR17 pKa = 11.84 KK18 pKa = 7.44 RR19 pKa = 11.84 VRR21 pKa = 11.84 RR22 pKa = 11.84 RR23 pKa = 11.84 WVGRR27 pKa = 11.84 RR28 pKa = 11.84 RR29 pKa = 11.84 RR30 pKa = 11.84 RR31 pKa = 11.84 RR32 pKa = 11.84 TYY34 pKa = 10.16 RR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 PWVRR41 pKa = 11.84 RR42 pKa = 11.84 RR43 pKa = 11.84 RR44 pKa = 11.84 PRR46 pKa = 11.84 RR47 pKa = 11.84 RR48 pKa = 11.84 RR49 pKa = 11.84 LLTLKK54 pKa = 9.77 QWQPQTNVRR63 pKa = 11.84 LTVRR67 pKa = 11.84 GILPFMVFGPKK78 pKa = 8.64 RR79 pKa = 11.84 TQRR82 pKa = 11.84 NYY84 pKa = 11.1 TMWQDD89 pKa = 3.29 TVPPEE94 pKa = 4.0 KK95 pKa = 10.07 EE96 pKa = 4.01 SYY98 pKa = 9.9 GGSFATYY105 pKa = 8.79 RR106 pKa = 11.84 FSMQTLYY113 pKa = 11.13 DD114 pKa = 3.18 EE115 pKa = 4.61 YY116 pKa = 10.91 MRR118 pKa = 11.84 LRR120 pKa = 11.84 NWWSRR125 pKa = 11.84 SNTHH129 pKa = 6.78 LDD131 pKa = 3.25 LVRR134 pKa = 11.84 YY135 pKa = 7.42 TGVSFRR141 pKa = 11.84 LYY143 pKa = 9.77 RR144 pKa = 11.84 HH145 pKa = 5.09 THH147 pKa = 5.66 RR148 pKa = 11.84 DD149 pKa = 3.59 YY150 pKa = 10.67 IVSYY154 pKa = 10.62 SLEE157 pKa = 4.18 TPMEE161 pKa = 4.17 SSIATHH167 pKa = 6.09 MSLHH171 pKa = 6.87 PMRR174 pKa = 11.84 QLFQSKK180 pKa = 9.58 HH181 pKa = 3.68 RR182 pKa = 11.84 TVPSFYY188 pKa = 9.32 TKK190 pKa = 10.39 PGGKK194 pKa = 9.35 PYY196 pKa = 8.97 ITLKK200 pKa = 10.6 ARR202 pKa = 11.84 PPRR205 pKa = 11.84 LMKK208 pKa = 8.46 TQWYY212 pKa = 7.71 FQHH215 pKa = 7.09 DD216 pKa = 4.17 FCNVGLILLRR226 pKa = 11.84 AACCNLTNPWMAPSAPTPCLTIYY249 pKa = 10.63 AISNGSHH256 pKa = 5.03 EE257 pKa = 4.69 HH258 pKa = 6.32 LSINADD264 pKa = 3.64 DD265 pKa = 3.47 THH267 pKa = 6.57 KK268 pKa = 10.66 QYY270 pKa = 10.44 RR271 pKa = 11.84 QHH273 pKa = 6.41 MLQKK277 pKa = 10.59 LYY279 pKa = 11.22 NNPQHH284 pKa = 7.04 LYY286 pKa = 8.17 NNHH289 pKa = 5.71 FRR291 pKa = 11.84 RR292 pKa = 11.84 HH293 pKa = 4.24 WSKK296 pKa = 10.95 VAPKK300 pKa = 10.59 KK301 pKa = 10.6 NGNQQTEE308 pKa = 4.1 PFTWEE313 pKa = 3.86 KK314 pKa = 10.7 VLKK317 pKa = 10.25 NDD319 pKa = 5.32 TIQTIQKK326 pKa = 10.13 NIYY329 pKa = 7.27 QEE331 pKa = 4.16 RR332 pKa = 11.84 LKK334 pKa = 9.81 EE335 pKa = 4.24 TQEE338 pKa = 4.04 VSTKK342 pKa = 9.8 RR343 pKa = 11.84 QQAGFRR349 pKa = 11.84 GNHH352 pKa = 5.81 NEE354 pKa = 4.6 DD355 pKa = 3.06 EE356 pKa = 4.58 TFNYY360 pKa = 8.79 TWGLYY365 pKa = 10.49 SSILLNTEE373 pKa = 3.59 PGFPEE378 pKa = 4.67 LSVPYY383 pKa = 10.38 SKK385 pKa = 10.75 VRR387 pKa = 11.84 YY388 pKa = 9.53 NPIADD393 pKa = 3.85 RR394 pKa = 11.84 GSGNAIWIEE403 pKa = 4.01 TLTKK407 pKa = 10.49 DD408 pKa = 3.09 NCHH411 pKa = 6.57 FSSQYY416 pKa = 10.09 EE417 pKa = 4.27 CLIKK421 pKa = 10.55 DD422 pKa = 3.57 APLWLCLYY430 pKa = 10.59 GYY432 pKa = 10.75 LDD434 pKa = 3.33 WCTKK438 pKa = 9.62 YY439 pKa = 10.94 KK440 pKa = 10.68 NNKK443 pKa = 7.8 EE444 pKa = 3.86 VYY446 pKa = 8.44 LNYY449 pKa = 10.05 RR450 pKa = 11.84 VGIRR454 pKa = 11.84 CPYY457 pKa = 8.97 TVPQLYY463 pKa = 10.52 SHH465 pKa = 7.11 DD466 pKa = 4.24 TPTKK470 pKa = 9.0 GWIPLSTNFMNGKK483 pKa = 8.46 MPGGSTTIPLRR494 pKa = 11.84 LQNNWYY500 pKa = 9.61 PMIYY504 pKa = 9.31 HH505 pKa = 5.24 QEE507 pKa = 3.79 EE508 pKa = 4.39 MIEE511 pKa = 4.16 EE512 pKa = 4.36 LVASGPFAPRR522 pKa = 11.84 DD523 pKa = 3.57 HH524 pKa = 6.85 GPVVTSSDD532 pKa = 2.66 ITIGYY537 pKa = 9.68 KK538 pKa = 10.03 FGFRR542 pKa = 11.84 LGGNYY547 pKa = 9.24 IAPKK551 pKa = 10.2 QVGDD555 pKa = 3.59 PCKK558 pKa = 10.49 QPVHH562 pKa = 6.38 EE563 pKa = 5.1 LPAPGGGDD571 pKa = 3.49 YY572 pKa = 10.89 LRR574 pKa = 11.84 EE575 pKa = 4.11 VQVTDD580 pKa = 3.56 PTRR583 pKa = 11.84 VGPHH587 pKa = 5.11 YY588 pKa = 10.23 QFHH591 pKa = 6.63 PWDD594 pKa = 3.49 LRR596 pKa = 11.84 RR597 pKa = 11.84 GLFSEE602 pKa = 4.68 KK603 pKa = 9.53 SLKK606 pKa = 9.91 RR607 pKa = 11.84 VRR609 pKa = 11.84 EE610 pKa = 4.14 DD611 pKa = 3.05 SDD613 pKa = 3.65 IEE615 pKa = 4.12 TDD617 pKa = 2.74 VWRR620 pKa = 11.84 PSKK623 pKa = 10.18 YY624 pKa = 10.61 AKK626 pKa = 9.62 PDD628 pKa = 3.42 PPAEE632 pKa = 4.1 GRR634 pKa = 11.84 GLAEE638 pKa = 4.27 LCSSVLRR645 pKa = 11.84 DD646 pKa = 3.72 LLQEE650 pKa = 4.34 TQHH653 pKa = 5.82 SQSPFSQAHH662 pKa = 6.1 PKK664 pKa = 9.03 TPEE667 pKa = 3.64 EE668 pKa = 4.39 TPTVDD673 pKa = 3.22 LQRR676 pKa = 11.84 LQRR679 pKa = 11.84 EE680 pKa = 4.08 IRR682 pKa = 11.84 QQQQQQSNLQKK693 pKa = 10.68 GIKK696 pKa = 9.89 NLVLEE701 pKa = 4.18 MVKK704 pKa = 9.81 YY705 pKa = 10.03 QRR707 pKa = 11.84 HH708 pKa = 6.03 LGVDD712 pKa = 3.2 PCLRR716 pKa = 3.96
Molecular weight: 84.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.289
IPC2_protein 9.56
IPC_protein 9.955
Toseland 10.014
ProMoST 9.853
Dawson 10.277
Bjellqvist 10.014
Wikipedia 10.496
Rodwell 10.511
Grimsley 10.379
Solomon 10.306
Lehninger 10.248
Nozaki 10.058
DTASelect 9.999
Thurlkill 10.116
EMBOSS 10.438
Sillero 10.204
Patrickios 9.151
IPC_peptide 10.292
IPC2_peptide 8.96
IPC2.peptide.svr19 8.565
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1232
146
716
308.0
35.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.006 ± 2.543
1.542 ± 0.19
4.058 ± 0.309
5.438 ± 0.85
2.922 ± 0.354
6.656 ± 2.086
3.003 ± 0.432
3.084 ± 0.684
4.302 ± 1.162
7.386 ± 0.856
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.786 ± 0.369
3.815 ± 0.931
9.821 ± 2.462
5.844 ± 0.256
10.227 ± 0.744
7.63 ± 2.315
6.331 ± 1.48
3.896 ± 0.855
2.273 ± 0.462
3.977 ± 1.193
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here