Mycobacterium virus CJW1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Kostyavirus

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 141 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q857S5|Q857S5_9CAUD Uncharacterized protein OS=Mycobacterium virus CJW1 OX=205869 GN=86 PE=4 SV=1
MM1 pKa = 7.33SRR3 pKa = 11.84EE4 pKa = 3.95FEE6 pKa = 4.07PAKK9 pKa = 10.23PWIARR14 pKa = 11.84DD15 pKa = 3.53GMPIYY20 pKa = 8.55PTASEE25 pKa = 5.38FEE27 pKa = 4.51TRR29 pKa = 11.84WDD31 pKa = 3.68ADD33 pKa = 3.15NTFPFFEE40 pKa = 5.07DD41 pKa = 3.83EE42 pKa = 4.03YY43 pKa = 11.08GYY45 pKa = 11.68GLFGYY50 pKa = 8.52GHH52 pKa = 5.83QDD54 pKa = 2.8KK55 pKa = 11.63AEE57 pKa = 4.14FVAMANRR64 pKa = 11.84YY65 pKa = 9.34NEE67 pKa = 4.12LCGVGIDD74 pKa = 4.27EE75 pKa = 5.06VPCTSDD81 pKa = 5.07DD82 pKa = 4.44VVHH85 pKa = 6.56CWAVVTDD92 pKa = 4.62PRR94 pKa = 11.84NEE96 pKa = 4.03DD97 pKa = 3.17DD98 pKa = 4.9GIRR101 pKa = 11.84FSWLGIDD108 pKa = 3.84ADD110 pKa = 4.7TYY112 pKa = 10.78GAWPLTWISYY122 pKa = 9.57

Molecular weight: 14.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q857Q5|Q857Q5_9CAUD HNHc domain-containing protein OS=Mycobacterium virus CJW1 OX=205869 GN=106 PE=4 SV=1
MM1 pKa = 7.02VRR3 pKa = 11.84RR4 pKa = 11.84GLVMVAQLHH13 pKa = 6.16KK14 pKa = 10.62GATAPSVRR22 pKa = 11.84HH23 pKa = 5.59HH24 pKa = 6.85RR25 pKa = 11.84LNGAHH30 pKa = 6.57MARR33 pKa = 11.84SSVRR37 pKa = 3.19

Molecular weight: 4.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

141

0

141

23698

32

1576

168.1

18.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.094 ± 0.41

1.182 ± 0.13

6.482 ± 0.193

6.655 ± 0.222

2.895 ± 0.145

8.802 ± 0.443

2.447 ± 0.156

4.389 ± 0.153

4.448 ± 0.207

8.123 ± 0.234

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.393 ± 0.128

3.329 ± 0.155

5.351 ± 0.19

3.249 ± 0.22

6.034 ± 0.287

5.722 ± 0.199

6.296 ± 0.245

7.089 ± 0.207

1.983 ± 0.121

3.038 ± 0.135
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski