Syntrophomonas wolfei subsp. wolfei (strain DSM 2245B / Goettingen)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Syntrophomonadaceae; Syntrophomonas; Syntrophomonas wolfei; Syntrophomonas wolfei subsp. wolfei

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2473 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q0AZN3|Q0AZN3_SYNWW Uncharacterized protein OS=Syntrophomonas wolfei subsp. wolfei (strain DSM 2245B / Goettingen) OX=335541 GN=Swol_0486 PE=4 SV=1
MM1 pKa = 7.36QDD3 pKa = 2.65ISEE6 pKa = 4.43KK7 pKa = 10.49VSYY10 pKa = 10.35LQGLSEE16 pKa = 4.16GLNIRR21 pKa = 11.84EE22 pKa = 4.56GSPQGKK28 pKa = 9.29IISGVLSVLDD38 pKa = 4.96EE39 pKa = 4.17IAEE42 pKa = 4.42SFLVMQDD49 pKa = 3.52RR50 pKa = 11.84FDD52 pKa = 3.73EE53 pKa = 4.37LQEE56 pKa = 4.18YY57 pKa = 8.81VEE59 pKa = 5.98SMDD62 pKa = 3.54TDD64 pKa = 3.74LQEE67 pKa = 5.94LEE69 pKa = 4.64EE70 pKa = 4.26KK71 pKa = 10.04WSEE74 pKa = 3.93EE75 pKa = 3.6LDD77 pKa = 3.52FAEE80 pKa = 5.88EE81 pKa = 4.42IRR83 pKa = 11.84CSHH86 pKa = 6.54CGEE89 pKa = 5.24LICFDD94 pKa = 4.66PEE96 pKa = 4.08VLDD99 pKa = 5.16DD100 pKa = 4.18EE101 pKa = 5.01DD102 pKa = 4.04VVEE105 pKa = 5.86IICPYY110 pKa = 8.99CDD112 pKa = 2.33EE113 pKa = 4.42VVFVNDD119 pKa = 4.54GSFDD123 pKa = 3.84YY124 pKa = 10.98QPSFIGDD131 pKa = 3.23EE132 pKa = 3.91KK133 pKa = 11.48GEE135 pKa = 4.12NEE137 pKa = 4.33EE138 pKa = 4.87HH139 pKa = 6.62SGAPII144 pKa = 3.71

Molecular weight: 16.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q0ATU2|RNPA_SYNWW Ribonuclease P protein component OS=Syntrophomonas wolfei subsp. wolfei (strain DSM 2245B / Goettingen) OX=335541 GN=rnpA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPKK8 pKa = 8.11NRR10 pKa = 11.84QRR12 pKa = 11.84KK13 pKa = 8.08RR14 pKa = 11.84EE15 pKa = 3.88HH16 pKa = 6.15GFRR19 pKa = 11.84ARR21 pKa = 11.84MASAGGRR28 pKa = 11.84KK29 pKa = 8.95VLANRR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 9.96KK37 pKa = 10.14GRR39 pKa = 11.84KK40 pKa = 9.21SISAA44 pKa = 3.61

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2473

0

2473

795412

35

2638

321.6

35.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.794 ± 0.055

1.184 ± 0.018

5.125 ± 0.038

7.501 ± 0.057

3.919 ± 0.029

7.148 ± 0.049

1.689 ± 0.018

7.681 ± 0.042

6.076 ± 0.043

10.51 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.665 ± 0.023

4.262 ± 0.042

3.921 ± 0.034

3.815 ± 0.028

5.256 ± 0.044

6.101 ± 0.037

4.399 ± 0.045

6.509 ± 0.037

0.972 ± 0.015

3.473 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski