Streptomyces griseoflavus Tu4000

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; Streptomyces griseoflavus

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6337 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D9XVG5|D9XVG5_9ACTN 50S ribosomal protein L5 OS=Streptomyces griseoflavus Tu4000 OX=467200 GN=rplE PE=3 SV=1
MM1 pKa = 7.9ALRR4 pKa = 11.84SIYY7 pKa = 9.62QEE9 pKa = 3.42EE10 pKa = 4.23HH11 pKa = 5.55MNRR14 pKa = 11.84SGRR17 pKa = 11.84MTRR20 pKa = 11.84LPLMAAMLVLAVACTSEE37 pKa = 4.23SSSDD41 pKa = 3.75GADD44 pKa = 3.32GQAASCAYY52 pKa = 9.69LVHH55 pKa = 5.93YY56 pKa = 9.24QDD58 pKa = 5.68RR59 pKa = 11.84SYY61 pKa = 11.71QDD63 pKa = 3.14VANVEE68 pKa = 4.49FTAGEE73 pKa = 4.12EE74 pKa = 4.21LGSATVPPCDD84 pKa = 3.59DD85 pKa = 3.29TGGQEE90 pKa = 4.37DD91 pKa = 4.55AEE93 pKa = 4.32QAEE96 pKa = 4.79DD97 pKa = 3.49TVTAYY102 pKa = 10.29RR103 pKa = 11.84VDD105 pKa = 4.54GISPDD110 pKa = 3.31VAVAVGDD117 pKa = 4.27SPDD120 pKa = 3.38DD121 pKa = 3.62TVFVVAYY128 pKa = 9.95SGGEE132 pKa = 3.91LPPEE136 pKa = 4.05VQKK139 pKa = 11.39LIDD142 pKa = 3.54RR143 pKa = 11.84SS144 pKa = 3.67

Molecular weight: 15.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D9XP02|D9XP02_9ACTN TetR-family transcriptional regulator OS=Streptomyces griseoflavus Tu4000 OX=467200 GN=SSRG_04623 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.75GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6337

0

6337

2042203

18

6111

322.3

34.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.136 ± 0.041

0.81 ± 0.009

6.072 ± 0.023

5.855 ± 0.027

2.768 ± 0.018

9.623 ± 0.03

2.339 ± 0.015

3.027 ± 0.022

2.173 ± 0.026

10.186 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.797 ± 0.013

1.79 ± 0.017

6.083 ± 0.025

2.643 ± 0.016

8.458 ± 0.041

5.033 ± 0.026

6.071 ± 0.03

8.504 ± 0.029

1.509 ± 0.012

2.124 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski