Clostridium sp. CAG:288

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1500 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7FNB0|R7FNB0_9CLOT Uncharacterized protein OS=Clostridium sp. CAG:288 OX=1262791 GN=BN588_01060 PE=4 SV=1
MM1 pKa = 8.16RR2 pKa = 11.84ILLDD6 pKa = 3.75EE7 pKa = 5.3DD8 pKa = 4.54DD9 pKa = 5.27RR10 pKa = 11.84SLSNAICTILKK21 pKa = 9.0YY22 pKa = 10.98NNYY25 pKa = 10.14SVDD28 pKa = 3.22AVYY31 pKa = 10.72DD32 pKa = 3.75GQEE35 pKa = 3.76ALDD38 pKa = 3.79YY39 pKa = 10.95LEE41 pKa = 5.23SNIYY45 pKa = 10.14DD46 pKa = 3.7AGDD49 pKa = 3.73DD50 pKa = 4.04YY51 pKa = 11.74EE52 pKa = 6.61LEE54 pKa = 4.52LEE56 pKa = 4.39LNGFNLISSNDD67 pKa = 3.38AAISITSGDD76 pKa = 4.38KK77 pKa = 9.45ITLSSKK83 pKa = 10.4KK84 pKa = 10.42

Molecular weight: 9.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7FP55|R7FP55_9CLOT Hypothetical alpha-dextrin endo-1 6-alpha-glucosidase OS=Clostridium sp. CAG:288 OX=1262791 GN=BN588_01244 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.14QPSKK9 pKa = 10.31RR10 pKa = 11.84KK11 pKa = 8.17HH12 pKa = 5.05QSVNGFRR19 pKa = 11.84ARR21 pKa = 11.84MATPSGRR28 pKa = 11.84KK29 pKa = 8.34VIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84HH36 pKa = 5.58KK37 pKa = 10.51GRR39 pKa = 11.84KK40 pKa = 8.42VLSAA44 pKa = 4.05

Molecular weight: 5.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1500

0

1500

488874

30

2491

325.9

36.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.725 ± 0.051

1.211 ± 0.021

6.011 ± 0.053

6.583 ± 0.061

4.757 ± 0.053

5.775 ± 0.06

1.413 ± 0.023

8.811 ± 0.077

8.691 ± 0.068

9.199 ± 0.071

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.204 ± 0.028

6.375 ± 0.06

2.76 ± 0.03

2.311 ± 0.029

3.093 ± 0.047

7.285 ± 0.078

5.697 ± 0.068

6.508 ± 0.056

0.631 ± 0.019

4.96 ± 0.056
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski