Faeces associated gemycircularvirus 6
Average proteome isoelectric point is 7.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|T1YTB5|T1YTB5_9VIRU Replication-associated protein OS=Faeces associated gemycircularvirus 6 OX=1391035 PE=3 SV=1
MM1 pKa = 7.4 LFVNSRR7 pKa = 11.84 YY8 pKa = 10.26 VLLTYY13 pKa = 9.15 AQCGDD18 pKa = 4.04 LDD20 pKa = 3.67 PWSVSNHH27 pKa = 4.96 LSALGAEE34 pKa = 4.68 CIVARR39 pKa = 11.84 EE40 pKa = 4.05 IHH42 pKa = 6.37 PTTGGIHH49 pKa = 5.94 LHH51 pKa = 6.55 CFADD55 pKa = 4.79 FNRR58 pKa = 11.84 KK59 pKa = 8.41 FRR61 pKa = 11.84 SRR63 pKa = 11.84 SARR66 pKa = 11.84 IFDD69 pKa = 3.35 VDD71 pKa = 3.29 GRR73 pKa = 11.84 HH74 pKa = 6.11 PNVVPSRR81 pKa = 11.84 GTPEE85 pKa = 3.6 KK86 pKa = 10.76 GYY88 pKa = 10.89 DD89 pKa = 3.47 YY90 pKa = 10.69 AIKK93 pKa = 10.86 DD94 pKa = 3.32 GDD96 pKa = 3.69 VRR98 pKa = 11.84 AGGLGRR104 pKa = 11.84 PAPRR108 pKa = 11.84 GGMSVGAHH116 pKa = 5.72 ALVNVAHH123 pKa = 6.8 LCEE126 pKa = 4.55 DD127 pKa = 3.34 TTEE130 pKa = 3.89 FLEE133 pKa = 5.06 LHH135 pKa = 7.02 DD136 pKa = 4.25 EE137 pKa = 4.15 MDD139 pKa = 3.55 RR140 pKa = 11.84 SGLIARR146 pKa = 11.84 FNNVRR151 pKa = 11.84 AYY153 pKa = 10.56 ADD155 pKa = 2.93 WRR157 pKa = 11.84 FRR159 pKa = 11.84 PEE161 pKa = 3.76 PVVYY165 pKa = 10.49 ASPDD169 pKa = 3.4 GVDD172 pKa = 4.02 FRR174 pKa = 11.84 SGSTDD179 pKa = 3.69 GRR181 pKa = 11.84 DD182 pKa = 3.16 DD183 pKa = 3.47 WLVQSRR189 pKa = 11.84 IGDD192 pKa = 3.6 EE193 pKa = 4.4 LPFVSAPVANAPFAGYY209 pKa = 9.78 IFCLLILYY217 pKa = 8.78 GPSLTGKK224 pKa = 5.82 TTWARR229 pKa = 11.84 SLGDD233 pKa = 3.68 HH234 pKa = 6.97 IFIQGVLSGKK244 pKa = 9.67 EE245 pKa = 3.84 VLNSDD250 pKa = 3.22 EE251 pKa = 4.26 SARR254 pKa = 11.84 YY255 pKa = 8.86 AVLDD259 pKa = 4.81 DD260 pKa = 3.6 IRR262 pKa = 11.84 GGLKK266 pKa = 10.03 FFPAWKK272 pKa = 9.72 DD273 pKa = 3.06 WLGGQRR279 pKa = 11.84 WISVKK284 pKa = 10.17 QMYY287 pKa = 9.78 RR288 pKa = 11.84 DD289 pKa = 4.32 PILLKK294 pKa = 10.04 WGRR297 pKa = 11.84 PCIWCANRR305 pKa = 11.84 DD306 pKa = 3.32 PRR308 pKa = 11.84 ADD310 pKa = 2.98 IRR312 pKa = 11.84 RR313 pKa = 11.84 SIDD316 pKa = 3.24 KK317 pKa = 10.8 DD318 pKa = 3.57 DD319 pKa = 3.85 GCFMEE324 pKa = 7.34 DD325 pKa = 4.31 DD326 pKa = 4.17 MDD328 pKa = 4.65 WINANCIFVYY338 pKa = 10.45 VDD340 pKa = 3.23 EE341 pKa = 4.72 SLVTFRR347 pKa = 11.84 ASTEE351 pKa = 3.77
Molecular weight: 39.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.511
IPC2_protein 5.474
IPC_protein 5.499
Toseland 5.779
ProMoST 5.677
Dawson 5.703
Bjellqvist 5.753
Wikipedia 5.703
Rodwell 5.664
Grimsley 5.881
Solomon 5.703
Lehninger 5.69
Nozaki 5.944
DTASelect 6.148
Thurlkill 6.071
EMBOSS 6.046
Sillero 6.033
Patrickios 4.088
IPC_peptide 5.715
IPC2_peptide 6.046
IPC2.peptide.svr19 5.994
Protein with the highest isoelectric point:
>tr|T1YTB5|T1YTB5_9VIRU Replication-associated protein OS=Faeces associated gemycircularvirus 6 OX=1391035 PE=3 SV=1
MM1 pKa = 7.53 PRR3 pKa = 11.84 FARR6 pKa = 11.84 RR7 pKa = 11.84 SSRR10 pKa = 11.84 FRR12 pKa = 11.84 SRR14 pKa = 11.84 KK15 pKa = 5.84 TARR18 pKa = 11.84 SSQRR22 pKa = 11.84 TRR24 pKa = 11.84 RR25 pKa = 11.84 PARR28 pKa = 11.84 RR29 pKa = 11.84 SRR31 pKa = 11.84 RR32 pKa = 11.84 TPRR35 pKa = 11.84 RR36 pKa = 11.84 SRR38 pKa = 11.84 RR39 pKa = 11.84 RR40 pKa = 11.84 GMSTRR45 pKa = 11.84 SILNKK50 pKa = 8.93 TSRR53 pKa = 11.84 KK54 pKa = 8.91 KK55 pKa = 10.53 RR56 pKa = 11.84 NGMLTISNTQATGNPDD72 pKa = 3.44 AFAQQPLTLIGSNTGGSIGVVHH94 pKa = 6.84 FRR96 pKa = 11.84 PTCMDD101 pKa = 4.27 LASPTGVANSIVNQSQRR118 pKa = 11.84 TSSTCFMRR126 pKa = 11.84 GLAEE130 pKa = 3.9 NLRR133 pKa = 11.84 IEE135 pKa = 4.43 TSSGNPWFHH144 pKa = 6.54 RR145 pKa = 11.84 RR146 pKa = 11.84 VCITSRR152 pKa = 11.84 DD153 pKa = 3.65 DD154 pKa = 3.47 NFRR157 pKa = 11.84 LVATGDD163 pKa = 3.42 NTGPQAAIMASGGIEE178 pKa = 4.11 TSNGWQRR185 pKa = 11.84 LAGNAQAGSEE195 pKa = 4.27 LDD197 pKa = 3.32 RR198 pKa = 11.84 TIVVWLEE205 pKa = 3.59 LLFKK209 pKa = 10.63 GRR211 pKa = 11.84 QGRR214 pKa = 11.84 DD215 pKa = 2.54 WDD217 pKa = 5.0 DD218 pKa = 4.29 IISAPVDD225 pKa = 3.42 TTRR228 pKa = 11.84 VDD230 pKa = 3.66 LKK232 pKa = 10.88 FDD234 pKa = 2.86 KK235 pKa = 10.73 TYY237 pKa = 10.4 IYY239 pKa = 10.65 RR240 pKa = 11.84 SGNEE244 pKa = 3.69 SGILKK249 pKa = 9.88 EE250 pKa = 3.96 KK251 pKa = 10.53 KK252 pKa = 9.06 LWHH255 pKa = 6.45 RR256 pKa = 11.84 MNKK259 pKa = 9.35 NLYY262 pKa = 10.22 FDD264 pKa = 5.5 DD265 pKa = 5.82 DD266 pKa = 3.91 EE267 pKa = 6.7 LGAGMEE273 pKa = 4.08 SHH275 pKa = 7.61 DD276 pKa = 4.56 YY277 pKa = 11.19 SVTDD281 pKa = 3.08 KK282 pKa = 11.02 RR283 pKa = 11.84 GNGDD287 pKa = 3.09 YY288 pKa = 10.74 HH289 pKa = 8.32 IFDD292 pKa = 4.99 FFSQGSSGTTDD303 pKa = 3.4 DD304 pKa = 3.63 RR305 pKa = 11.84 LKK307 pKa = 11.18 VRR309 pKa = 11.84 FTSTLYY315 pKa = 9.36 WHH317 pKa = 7.08 EE318 pKa = 4.2 KK319 pKa = 9.32
Molecular weight: 36.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.336
IPC2_protein 9.677
IPC_protein 10.716
Toseland 10.906
ProMoST 10.833
Dawson 10.965
Bjellqvist 10.745
Wikipedia 11.242
Rodwell 11.023
Grimsley 11.008
Solomon 11.184
Lehninger 11.14
Nozaki 10.891
DTASelect 10.745
Thurlkill 10.891
EMBOSS 11.33
Sillero 10.906
Patrickios 10.73
IPC_peptide 11.199
IPC2_peptide 9.897
IPC2.peptide.svr19 8.77
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
670
319
351
335.0
37.74
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.866 ± 0.917
1.791 ± 0.638
8.06 ± 1.107
3.582 ± 0.335
4.776 ± 0.29
8.657 ± 0.145
2.239 ± 0.268
5.075 ± 0.279
3.582 ± 0.605
7.164 ± 0.671
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.09 ± 0.314
4.328 ± 0.75
4.179 ± 0.783
2.388 ± 0.795
10.448 ± 1.568
8.358 ± 1.489
5.97 ± 2.105
5.522 ± 1.32
2.388 ± 0.38
2.537 ± 0.492
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here