Gordonia phage Nyceirae

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Nyceiraevirus; Gordonia virus Nyceirae

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1C9EI22|A0A1C9EI22_9CAUD Uncharacterized protein OS=Gordonia phage Nyceirae OX=1887651 GN=44 PE=4 SV=1
MM1 pKa = 7.85RR2 pKa = 11.84ISTAIAAATISAGLLVTSCGGGGSDD27 pKa = 4.42SAGGGADD34 pKa = 3.12QGFAGSGTISAMVTYY49 pKa = 8.34PQIGAPDD56 pKa = 3.66AEE58 pKa = 4.32ACVVSSASGTIQRR71 pKa = 11.84GTSLIVRR78 pKa = 11.84TQDD81 pKa = 3.06GEE83 pKa = 4.38LVGEE87 pKa = 4.42SEE89 pKa = 4.64LPQGSLLKK97 pKa = 10.83DD98 pKa = 3.34EE99 pKa = 5.05SGLVGWCEE107 pKa = 3.49WRR109 pKa = 11.84FSVEE113 pKa = 3.77VTQRR117 pKa = 11.84QPNYY121 pKa = 9.79QIEE124 pKa = 4.41VGDD127 pKa = 4.24GSVSTAGQIEE137 pKa = 4.89LSTGEE142 pKa = 4.22FKK144 pKa = 11.05FLVPASGGG152 pKa = 3.46

Molecular weight: 15.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1C9EI02|A0A1C9EI02_9CAUD Uncharacterized protein OS=Gordonia phage Nyceirae OX=1887651 GN=48 PE=4 SV=1
MM1 pKa = 7.43SRR3 pKa = 11.84TRR5 pKa = 11.84PPRR8 pKa = 11.84TCRR11 pKa = 11.84YY12 pKa = 9.41CRR14 pKa = 11.84LPVLWWRR21 pKa = 11.84NANRR25 pKa = 11.84DD26 pKa = 2.91GSICVDD32 pKa = 3.67VSADD36 pKa = 4.47DD37 pKa = 3.71NGTVQKK43 pKa = 10.91VVTHH47 pKa = 6.55PAGEE51 pKa = 4.16RR52 pKa = 11.84PVVWGRR58 pKa = 11.84RR59 pKa = 11.84LTGLDD64 pKa = 3.14LATAVEE70 pKa = 4.28AGEE73 pKa = 4.3QLFTLHH79 pKa = 6.52ATTCSARR86 pKa = 11.84KK87 pKa = 8.78QRR89 pKa = 11.84NPKK92 pKa = 10.12PEE94 pKa = 4.17GLQIAWPNHH103 pKa = 4.35NPRR106 pKa = 11.84RR107 pKa = 3.92

Molecular weight: 12.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

60

0

60

13331

47

1835

222.2

24.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.912 ± 0.703

0.833 ± 0.167

6.774 ± 0.238

5.596 ± 0.273

2.513 ± 0.167

9.369 ± 0.599

1.845 ± 0.151

3.998 ± 0.189

2.918 ± 0.239

8.041 ± 0.276

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.22 ± 0.12

2.745 ± 0.211

6.031 ± 0.433

3.788 ± 0.311

7.689 ± 0.429

5.633 ± 0.25

6.594 ± 0.324

7.216 ± 0.347

2.115 ± 0.208

2.168 ± 0.173
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski