Enhygromyxa salina
Average proteome isoelectric point is 5.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6943 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2S9XLW3|A0A2S9XLW3_9DELT Uncharacterized protein OS=Enhygromyxa salina OX=215803 GN=ENSA5_42460 PE=4 SV=1
MM1 pKa = 7.24 TNSLRR6 pKa = 11.84 LLFGTCLILSPIACGDD22 pKa = 3.39 SSATDD27 pKa = 3.63 GEE29 pKa = 4.92 GASEE33 pKa = 4.0 TGYY36 pKa = 11.53 GDD38 pKa = 3.51 GDD40 pKa = 4.01 GDD42 pKa = 4.31 GDD44 pKa = 4.42 PGDD47 pKa = 4.42 GDD49 pKa = 4.49 GDD51 pKa = 4.17 PGDD54 pKa = 4.42 GDD56 pKa = 4.49 GDD58 pKa = 4.22 PGDD61 pKa = 4.85 GDD63 pKa = 3.96 TGDD66 pKa = 4.53 GDD68 pKa = 4.05 TGDD71 pKa = 4.61 GDD73 pKa = 4.05 TGDD76 pKa = 4.61 GDD78 pKa = 4.0 TGDD81 pKa = 4.7 GDD83 pKa = 4.2 MDD85 pKa = 4.78 CGPIGEE91 pKa = 5.24 PPPEE95 pKa = 4.8 APIVMVAPADD105 pKa = 3.89 AGALPQMVAEE115 pKa = 4.88 AEE117 pKa = 4.26 PNTTFMFADD126 pKa = 4.18 GTYY129 pKa = 10.0 DD130 pKa = 3.78 LSGGSQIQVRR140 pKa = 11.84 TPGLRR145 pKa = 11.84 FVGASNDD152 pKa = 3.34 RR153 pKa = 11.84 DD154 pKa = 4.02 AVILDD159 pKa = 3.42 ADD161 pKa = 3.98 YY162 pKa = 11.31 GIGEE166 pKa = 4.39 IFLVTASDD174 pKa = 3.4 TTIAHH179 pKa = 5.63 MTLQRR184 pKa = 11.84 ATWHH188 pKa = 6.88 PIHH191 pKa = 6.13 VTGGDD196 pKa = 3.41 QANTEE201 pKa = 3.71 NTMIYY206 pKa = 10.1 DD207 pKa = 3.52 VAVIDD212 pKa = 4.88 PGEE215 pKa = 3.8 QAIKK219 pKa = 10.45 INASGAGYY227 pKa = 10.05 FSDD230 pKa = 4.4 YY231 pKa = 10.07 GTVACSSVVMTDD243 pKa = 2.88 AGRR246 pKa = 11.84 AQVQNCYY253 pKa = 9.84 TGGVDD258 pKa = 3.13 AHH260 pKa = 6.16 SARR263 pKa = 11.84 GWVVRR268 pKa = 11.84 DD269 pKa = 3.44 NYY271 pKa = 10.91 FEE273 pKa = 5.64 GFWCDD278 pKa = 3.09 VGLSEE283 pKa = 5.32 HH284 pKa = 6.9 AVHH287 pKa = 6.94 FWITGRR293 pKa = 11.84 DD294 pKa = 3.36 TVVEE298 pKa = 4.19 RR299 pKa = 11.84 NTIVDD304 pKa = 3.87 CARR307 pKa = 11.84 GVGFGLGQNGNGKK320 pKa = 8.88 QRR322 pKa = 11.84 VYY324 pKa = 11.42 DD325 pKa = 4.56 DD326 pKa = 5.12 DD327 pKa = 4.56 PCPGADD333 pKa = 3.53 YY334 pKa = 10.5 LGHH337 pKa = 6.06 VDD339 pKa = 5.09 GVIRR343 pKa = 11.84 DD344 pKa = 3.69 NMIFAGRR351 pKa = 11.84 PEE353 pKa = 4.46 LFASQSGFDD362 pKa = 3.46 SGVALEE368 pKa = 4.31 QACGTVVAHH377 pKa = 5.58 NTVASLQPPFVAMEE391 pKa = 3.84 YY392 pKa = 10.31 RR393 pKa = 11.84 FPNTDD398 pKa = 2.64 ATIVNNLTTHH408 pKa = 6.83 GIVMRR413 pKa = 11.84 DD414 pKa = 3.16 GGQASLVGNLEE425 pKa = 4.18 DD426 pKa = 3.71 QGLEE430 pKa = 4.14 HH431 pKa = 6.77 FVDD434 pKa = 3.72 AAGGDD439 pKa = 3.55 LHH441 pKa = 8.33 LVEE444 pKa = 5.9 GSAAVDD450 pKa = 3.29 AGDD453 pKa = 3.82 PSGVDD458 pKa = 3.39 WAPTDD463 pKa = 3.59 YY464 pKa = 11.48 DD465 pKa = 3.49 GDD467 pKa = 4.1 ARR469 pKa = 11.84 DD470 pKa = 3.69 GAPDD474 pKa = 3.38 VGADD478 pKa = 3.78 EE479 pKa = 5.22 LPP481 pKa = 3.88
Molecular weight: 49.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.671
IPC2_protein 3.694
IPC_protein 3.745
Toseland 3.503
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.719
Rodwell 3.567
Grimsley 3.414
Solomon 3.757
Lehninger 3.706
Nozaki 3.859
DTASelect 4.164
Thurlkill 3.567
EMBOSS 3.719
Sillero 3.872
Patrickios 1.43
IPC_peptide 3.745
IPC2_peptide 3.846
IPC2.peptide.svr19 3.788
Protein with the highest isoelectric point:
>tr|A0A2S9XRF7|A0A2S9XRF7_9DELT 7 8-dihydroneopterin aldolase OS=Enhygromyxa salina OX=215803 GN=folB PE=3 SV=1
MM1 pKa = 7.74 SKK3 pKa = 8.96 RR4 pKa = 11.84 TYY6 pKa = 9.96 QPHH9 pKa = 5.73 NLSRR13 pKa = 11.84 ARR15 pKa = 11.84 THH17 pKa = 6.36 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MATRR26 pKa = 11.84 NGRR29 pKa = 11.84 KK30 pKa = 9.13 IIAARR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 RR38 pKa = 11.84 GRR40 pKa = 11.84 KK41 pKa = 9.12 RR42 pKa = 11.84 LGTTTGGKK50 pKa = 9.53
Molecular weight: 5.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.463
IPC2_protein 11.184
IPC_protein 12.588
Toseland 12.749
ProMoST 13.247
Dawson 12.749
Bjellqvist 12.749
Wikipedia 13.217
Rodwell 12.384
Grimsley 12.793
Solomon 13.247
Lehninger 13.144
Nozaki 12.749
DTASelect 12.749
Thurlkill 12.749
EMBOSS 13.247
Sillero 12.749
Patrickios 12.106
IPC_peptide 13.247
IPC2_peptide 12.237
IPC2.peptide.svr19 9.106
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6943
0
6943
2649706
29
7134
381.6
41.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.235 ± 0.051
1.274 ± 0.016
6.693 ± 0.032
7.16 ± 0.035
3.053 ± 0.022
8.983 ± 0.041
2.165 ± 0.013
3.813 ± 0.019
2.315 ± 0.028
10.6 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.782 ± 0.012
2.003 ± 0.018
5.84 ± 0.024
2.936 ± 0.017
7.864 ± 0.043
5.479 ± 0.021
4.809 ± 0.021
7.488 ± 0.026
1.507 ± 0.012
2.0 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here