Sulfitobacter sp. SK011

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Sulfitobacter; unclassified Sulfitobacter

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4006 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345QAA8|A0A345QAA8_9RHOB 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) OS=Sulfitobacter sp. SK011 OX=1389004 GN=ispG PE=3 SV=1
MM1 pKa = 7.8TDD3 pKa = 2.92QTHH6 pKa = 6.54PLEE9 pKa = 4.69GTPLIAPSSTDD20 pKa = 3.01HH21 pKa = 6.94PLYY24 pKa = 10.06EE25 pKa = 4.54AVVEE29 pKa = 4.41ACRR32 pKa = 11.84TVYY35 pKa = 10.74DD36 pKa = 3.74PEE38 pKa = 5.09IPVNIYY44 pKa = 10.39EE45 pKa = 4.82LGLIYY50 pKa = 10.36TIDD53 pKa = 3.42INDD56 pKa = 3.54QNEE59 pKa = 3.77VDD61 pKa = 3.64IKK63 pKa = 10.75MSLTAPGCPVAGEE76 pKa = 4.15MPGWVADD83 pKa = 3.82AVEE86 pKa = 4.32PLAGVKK92 pKa = 9.89QVDD95 pKa = 3.8VALVWEE101 pKa = 5.18PPWGMDD107 pKa = 3.35MMSDD111 pKa = 3.68EE112 pKa = 4.89ARR114 pKa = 11.84LEE116 pKa = 4.14LGFMM120 pKa = 4.54

Molecular weight: 13.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345QEA7|A0A345QEA7_9RHOB Uncharacterized protein OS=Sulfitobacter sp. SK011 OX=1389004 GN=C1J02_18250 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.81IINARR34 pKa = 11.84RR35 pKa = 11.84AQGRR39 pKa = 11.84KK40 pKa = 8.52EE41 pKa = 3.76LSAA44 pKa = 4.93

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4006

0

4006

1234747

36

3038

308.2

33.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.887 ± 0.045

0.95 ± 0.012

6.231 ± 0.03

5.424 ± 0.034

3.885 ± 0.027

8.472 ± 0.037

2.164 ± 0.018

5.554 ± 0.031

3.596 ± 0.028

9.812 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.97 ± 0.018

2.866 ± 0.018

4.9 ± 0.024

3.345 ± 0.02

6.137 ± 0.03

5.269 ± 0.024

5.583 ± 0.024

7.266 ± 0.03

1.413 ± 0.018

2.276 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski