Legionella beliardensis
Average proteome isoelectric point is 6.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3101 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A378HYG2|A0A378HYG2_9GAMM Uncharacterized protein OS=Legionella beliardensis OX=91822 GN=NCTC13315_00461 PE=4 SV=1
MM1 pKa = 7.61 LNLNYY6 pKa = 10.79 GIICDD11 pKa = 3.67 ILIKK15 pKa = 10.56 ARR17 pKa = 11.84 QFQAKK22 pKa = 9.44 EE23 pKa = 3.94 EE24 pKa = 4.25 VSFPEE29 pKa = 4.07 MTDD32 pKa = 4.15 DD33 pKa = 3.52 MDD35 pKa = 4.36 ASYY38 pKa = 11.72 VLADD42 pKa = 3.68 YY43 pKa = 11.46 ADD45 pKa = 3.88 DD46 pKa = 4.4 LVYY49 pKa = 10.89 QEE51 pKa = 4.72 VVGAINNLRR60 pKa = 11.84 PDD62 pKa = 3.48 QQATLVALMYY72 pKa = 10.33 VGRR75 pKa = 11.84 GDD77 pKa = 3.63 YY78 pKa = 9.81 TVEE81 pKa = 3.48 EE82 pKa = 4.32 WGEE85 pKa = 3.7 AFAFAEE91 pKa = 4.4 EE92 pKa = 4.48 RR93 pKa = 11.84 WTEE96 pKa = 3.79 QTGEE100 pKa = 4.18 YY101 pKa = 10.37 LLSRR105 pKa = 11.84 PTMPDD110 pKa = 3.39 DD111 pKa = 3.95 IEE113 pKa = 6.05 RR114 pKa = 11.84 GLDD117 pKa = 3.33 ALSIACEE124 pKa = 3.83
Molecular weight: 14.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.753
IPC2_protein 3.846
IPC_protein 3.808
Toseland 3.605
ProMoST 3.923
Dawson 3.783
Bjellqvist 3.986
Wikipedia 3.694
Rodwell 3.63
Grimsley 3.516
Solomon 3.77
Lehninger 3.719
Nozaki 3.897
DTASelect 4.075
Thurlkill 3.656
EMBOSS 3.706
Sillero 3.923
Patrickios 1.863
IPC_peptide 3.77
IPC2_peptide 3.897
IPC2.peptide.svr19 3.839
Protein with the highest isoelectric point:
>tr|A0A378I4Z4|A0A378I4Z4_9GAMM LysR family transcriptional regulator OS=Legionella beliardensis OX=91822 GN=iciA PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.28 QPSKK9 pKa = 10.41 LKK11 pKa = 10.45 RR12 pKa = 11.84 KK13 pKa = 8.84 RR14 pKa = 11.84 DD15 pKa = 3.18 HH16 pKa = 7.01 GFRR19 pKa = 11.84 QRR21 pKa = 11.84 MATRR25 pKa = 11.84 GGRR28 pKa = 11.84 LVLKK32 pKa = 10.32 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.84 GRR39 pKa = 11.84 KK40 pKa = 8.85 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.356
IPC2_protein 10.906
IPC_protein 12.325
Toseland 12.486
ProMoST 12.983
Dawson 12.486
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.223
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 11.959
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.069
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3101
0
3101
1004299
29
2171
323.9
36.39
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.003 ± 0.047
1.187 ± 0.016
4.72 ± 0.032
5.7 ± 0.048
4.507 ± 0.033
5.789 ± 0.053
2.425 ± 0.025
7.563 ± 0.039
6.304 ± 0.051
11.258 ± 0.063
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.165 ± 0.019
5.223 ± 0.037
4.112 ± 0.025
4.746 ± 0.036
4.137 ± 0.029
6.263 ± 0.03
5.486 ± 0.036
5.679 ± 0.04
1.128 ± 0.016
3.604 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here