Legionella beliardensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales; Legionellaceae; Legionella

Average proteome isoelectric point is 6.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3101 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A378HYG2|A0A378HYG2_9GAMM Uncharacterized protein OS=Legionella beliardensis OX=91822 GN=NCTC13315_00461 PE=4 SV=1
MM1 pKa = 7.61LNLNYY6 pKa = 10.79GIICDD11 pKa = 3.67ILIKK15 pKa = 10.56ARR17 pKa = 11.84QFQAKK22 pKa = 9.44EE23 pKa = 3.94EE24 pKa = 4.25VSFPEE29 pKa = 4.07MTDD32 pKa = 4.15DD33 pKa = 3.52MDD35 pKa = 4.36ASYY38 pKa = 11.72VLADD42 pKa = 3.68YY43 pKa = 11.46ADD45 pKa = 3.88DD46 pKa = 4.4LVYY49 pKa = 10.89QEE51 pKa = 4.72VVGAINNLRR60 pKa = 11.84PDD62 pKa = 3.48QQATLVALMYY72 pKa = 10.33VGRR75 pKa = 11.84GDD77 pKa = 3.63YY78 pKa = 9.81TVEE81 pKa = 3.48EE82 pKa = 4.32WGEE85 pKa = 3.7AFAFAEE91 pKa = 4.4EE92 pKa = 4.48RR93 pKa = 11.84WTEE96 pKa = 3.79QTGEE100 pKa = 4.18YY101 pKa = 10.37LLSRR105 pKa = 11.84PTMPDD110 pKa = 3.39DD111 pKa = 3.95IEE113 pKa = 6.05RR114 pKa = 11.84GLDD117 pKa = 3.33ALSIACEE124 pKa = 3.83

Molecular weight:
14.07 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A378I4Z4|A0A378I4Z4_9GAMM LysR family transcriptional regulator OS=Legionella beliardensis OX=91822 GN=iciA PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.28QPSKK9 pKa = 10.41LKK11 pKa = 10.45RR12 pKa = 11.84KK13 pKa = 8.84RR14 pKa = 11.84DD15 pKa = 3.18HH16 pKa = 7.01GFRR19 pKa = 11.84QRR21 pKa = 11.84MATRR25 pKa = 11.84GGRR28 pKa = 11.84LVLKK32 pKa = 10.32RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.84GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight:
5.34 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3101

0

3101

1004299

29

2171

323.9

36.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.003 ± 0.047

1.187 ± 0.016

4.72 ± 0.032

5.7 ± 0.048

4.507 ± 0.033

5.789 ± 0.053

2.425 ± 0.025

7.563 ± 0.039

6.304 ± 0.051

11.258 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.165 ± 0.019

5.223 ± 0.037

4.112 ± 0.025

4.746 ± 0.036

4.137 ± 0.029

6.263 ± 0.03

5.486 ± 0.036

5.679 ± 0.04

1.128 ± 0.016

3.604 ± 0.027

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski