Amino acid dipepetide frequency for Escherichia phage OLB145

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.627AlaAla: 7.627 ± 1.02
0.594AlaCys: 0.594 ± 0.183
4.293AlaAsp: 4.293 ± 0.517
5.663AlaGlu: 5.663 ± 0.606
2.649AlaPhe: 2.649 ± 0.37
6.257AlaGly: 6.257 ± 0.731
1.142AlaHis: 1.142 ± 0.27
5.206AlaIle: 5.206 ± 0.598
5.435AlaLys: 5.435 ± 0.599
8.632AlaLeu: 8.632 ± 0.809
2.695AlaMet: 2.695 ± 0.347
4.247AlaAsn: 4.247 ± 0.461
3.014AlaPro: 3.014 ± 0.461
3.928AlaGln: 3.928 ± 0.682
3.334AlaArg: 3.334 ± 0.515
4.156AlaSer: 4.156 ± 0.449
6.028AlaThr: 6.028 ± 0.564
5.435AlaVal: 5.435 ± 0.533
1.005AlaTrp: 1.005 ± 0.235
3.836AlaTyr: 3.836 ± 0.411
0.0AlaXaa: 0.0 ± 0.0
Cys
0.502CysAla: 0.502 ± 0.148
0.137CysCys: 0.137 ± 0.071
0.457CysAsp: 0.457 ± 0.152
0.365CysGlu: 0.365 ± 0.137
0.274CysPhe: 0.274 ± 0.123
0.822CysGly: 0.822 ± 0.256
0.274CysHis: 0.274 ± 0.127
0.731CysIle: 0.731 ± 0.221
1.005CysLys: 1.005 ± 0.27
0.776CysLeu: 0.776 ± 0.235
0.183CysMet: 0.183 ± 0.084
0.457CysAsn: 0.457 ± 0.156
0.137CysPro: 0.137 ± 0.08
0.32CysGln: 0.32 ± 0.146
0.411CysArg: 0.411 ± 0.187
0.594CysSer: 0.594 ± 0.182
0.639CysThr: 0.639 ± 0.179
0.457CysVal: 0.457 ± 0.168
0.137CysTrp: 0.137 ± 0.079
0.228CysTyr: 0.228 ± 0.112
0.0CysXaa: 0.0 ± 0.0
Asp
5.252AspAla: 5.252 ± 0.443
0.731AspCys: 0.731 ± 0.239
3.243AspAsp: 3.243 ± 0.491
3.288AspGlu: 3.288 ± 0.484
2.009AspPhe: 2.009 ± 0.306
3.745AspGly: 3.745 ± 0.459
0.776AspHis: 0.776 ± 0.189
4.476AspIle: 4.476 ± 0.485
4.11AspLys: 4.11 ± 0.348
4.293AspLeu: 4.293 ± 0.43
1.416AspMet: 1.416 ± 0.182
2.375AspAsn: 2.375 ± 0.374
3.197AspPro: 3.197 ± 0.398
2.009AspGln: 2.009 ± 0.282
2.284AspArg: 2.284 ± 0.242
3.654AspSer: 3.654 ± 0.414
3.106AspThr: 3.106 ± 0.459
4.065AspVal: 4.065 ± 0.445
1.096AspTrp: 1.096 ± 0.245
2.512AspTyr: 2.512 ± 0.374
0.0AspXaa: 0.0 ± 0.0
Glu
5.617GluAla: 5.617 ± 0.63
0.502GluCys: 0.502 ± 0.161
3.928GluAsp: 3.928 ± 0.471
5.435GluGlu: 5.435 ± 0.597
2.603GluPhe: 2.603 ± 0.364
4.019GluGly: 4.019 ± 0.506
1.598GluHis: 1.598 ± 0.214
3.654GluIle: 3.654 ± 0.41
3.928GluLys: 3.928 ± 0.461
5.709GluLeu: 5.709 ± 0.536
2.055GluMet: 2.055 ± 0.268
2.969GluAsn: 2.969 ± 0.325
2.877GluPro: 2.877 ± 0.386
3.699GluGln: 3.699 ± 0.495
2.375GluArg: 2.375 ± 0.324
3.471GluSer: 3.471 ± 0.37
3.334GluThr: 3.334 ± 0.334
4.704GluVal: 4.704 ± 0.561
0.959GluTrp: 0.959 ± 0.219
2.832GluTyr: 2.832 ± 0.376
0.0GluXaa: 0.0 ± 0.0
Phe
2.421PheAla: 2.421 ± 0.287
0.365PheCys: 0.365 ± 0.157
2.147PheAsp: 2.147 ± 0.285
1.598PheGlu: 1.598 ± 0.234
1.187PhePhe: 1.187 ± 0.246
2.329PheGly: 2.329 ± 0.313
0.685PheHis: 0.685 ± 0.157
2.375PheIle: 2.375 ± 0.382
2.284PheLys: 2.284 ± 0.352
2.603PheLeu: 2.603 ± 0.376
1.644PheMet: 1.644 ± 0.243
2.421PheAsn: 2.421 ± 0.38
1.096PhePro: 1.096 ± 0.297
1.416PheGln: 1.416 ± 0.262
1.598PheArg: 1.598 ± 0.251
2.101PheSer: 2.101 ± 0.324
2.969PheThr: 2.969 ± 0.476
2.284PheVal: 2.284 ± 0.323
0.32PheTrp: 0.32 ± 0.127
1.553PheTyr: 1.553 ± 0.225
0.0PheXaa: 0.0 ± 0.0
Gly
5.572GlyAla: 5.572 ± 0.786
0.548GlyCys: 0.548 ± 0.167
2.923GlyAsp: 2.923 ± 0.431
3.654GlyGlu: 3.654 ± 0.371
2.923GlyPhe: 2.923 ± 0.427
3.151GlyGly: 3.151 ± 0.529
1.142GlyHis: 1.142 ± 0.256
4.521GlyIle: 4.521 ± 0.394
5.298GlyLys: 5.298 ± 0.504
4.978GlyLeu: 4.978 ± 0.581
2.192GlyMet: 2.192 ± 0.238
4.43GlyAsn: 4.43 ± 0.441
0.685GlyPro: 0.685 ± 0.142
2.329GlyGln: 2.329 ± 0.41
2.558GlyArg: 2.558 ± 0.376
4.613GlySer: 4.613 ± 0.371
4.704GlyThr: 4.704 ± 0.442
3.973GlyVal: 3.973 ± 0.496
0.868GlyTrp: 0.868 ± 0.221
2.877GlyTyr: 2.877 ± 0.327
0.0GlyXaa: 0.0 ± 0.0
His
1.324HisAla: 1.324 ± 0.274
0.274HisCys: 0.274 ± 0.111
0.868HisAsp: 0.868 ± 0.233
1.279HisGlu: 1.279 ± 0.259
0.822HisPhe: 0.822 ± 0.191
1.096HisGly: 1.096 ± 0.261
0.457HisHis: 0.457 ± 0.153
1.187HisIle: 1.187 ± 0.214
0.959HisLys: 0.959 ± 0.144
1.964HisLeu: 1.964 ± 0.313
0.685HisMet: 0.685 ± 0.172
0.594HisAsn: 0.594 ± 0.191
0.822HisPro: 0.822 ± 0.211
0.731HisGln: 0.731 ± 0.208
1.142HisArg: 1.142 ± 0.278
1.096HisSer: 1.096 ± 0.223
0.776HisThr: 0.776 ± 0.168
1.142HisVal: 1.142 ± 0.244
0.365HisTrp: 0.365 ± 0.114
0.868HisTyr: 0.868 ± 0.232
0.0HisXaa: 0.0 ± 0.0
Ile
4.75IleAla: 4.75 ± 0.586
0.457IleCys: 0.457 ± 0.188
4.247IleAsp: 4.247 ± 0.498
4.202IleGlu: 4.202 ± 0.332
1.735IlePhe: 1.735 ± 0.352
3.151IleGly: 3.151 ± 0.37
1.324IleHis: 1.324 ± 0.256
3.654IleIle: 3.654 ± 0.566
4.795IleLys: 4.795 ± 0.426
3.791IleLeu: 3.791 ± 0.41
1.37IleMet: 1.37 ± 0.284
3.197IleAsn: 3.197 ± 0.326
3.425IlePro: 3.425 ± 0.482
2.375IleGln: 2.375 ± 0.309
3.243IleArg: 3.243 ± 0.408
3.288IleSer: 3.288 ± 0.354
4.521IleThr: 4.521 ± 0.466
3.699IleVal: 3.699 ± 0.574
0.502IleTrp: 0.502 ± 0.161
2.375IleTyr: 2.375 ± 0.301
0.0IleXaa: 0.0 ± 0.0
Lys
7.307LysAla: 7.307 ± 0.602
0.594LysCys: 0.594 ± 0.176
4.476LysAsp: 4.476 ± 0.53
4.567LysGlu: 4.567 ± 0.464
2.147LysPhe: 2.147 ± 0.306
3.654LysGly: 3.654 ± 0.416
1.37LysHis: 1.37 ± 0.28
2.877LysIle: 2.877 ± 0.323
4.247LysLys: 4.247 ± 0.534
6.394LysLeu: 6.394 ± 0.571
2.147LysMet: 2.147 ± 0.317
2.877LysAsn: 2.877 ± 0.366
2.603LysPro: 2.603 ± 0.432
3.425LysGln: 3.425 ± 0.388
2.969LysArg: 2.969 ± 0.386
3.517LysSer: 3.517 ± 0.388
4.384LysThr: 4.384 ± 0.448
4.521LysVal: 4.521 ± 0.377
0.959LysTrp: 0.959 ± 0.26
1.781LysTyr: 1.781 ± 0.292
0.0LysXaa: 0.0 ± 0.0
Leu
7.353LeuAla: 7.353 ± 0.681
0.868LeuCys: 0.868 ± 0.256
5.754LeuAsp: 5.754 ± 0.553
4.75LeuGlu: 4.75 ± 0.541
3.334LeuPhe: 3.334 ± 0.379
6.028LeuGly: 6.028 ± 0.634
1.37LeuHis: 1.37 ± 0.324
4.019LeuIle: 4.019 ± 0.481
5.069LeuLys: 5.069 ± 0.5
6.394LeuLeu: 6.394 ± 0.606
2.284LeuMet: 2.284 ± 0.439
5.206LeuAsn: 5.206 ± 0.551
3.517LeuPro: 3.517 ± 0.353
3.243LeuGln: 3.243 ± 0.292
4.019LeuArg: 4.019 ± 0.49
4.75LeuSer: 4.75 ± 0.518
6.074LeuThr: 6.074 ± 0.512
5.526LeuVal: 5.526 ± 0.548
0.685LeuTrp: 0.685 ± 0.172
2.009LeuTyr: 2.009 ± 0.325
0.0LeuXaa: 0.0 ± 0.0
Met
2.558MetAla: 2.558 ± 0.381
0.091MetCys: 0.091 ± 0.079
1.324MetAsp: 1.324 ± 0.333
2.421MetGlu: 2.421 ± 0.314
0.776MetPhe: 0.776 ± 0.224
1.461MetGly: 1.461 ± 0.307
0.365MetHis: 0.365 ± 0.112
1.37MetIle: 1.37 ± 0.218
2.101MetLys: 2.101 ± 0.278
2.055MetLeu: 2.055 ± 0.285
0.731MetMet: 0.731 ± 0.145
1.644MetAsn: 1.644 ± 0.284
1.553MetPro: 1.553 ± 0.339
1.735MetGln: 1.735 ± 0.255
1.233MetArg: 1.233 ± 0.195
2.74MetSer: 2.74 ± 0.298
2.558MetThr: 2.558 ± 0.348
1.918MetVal: 1.918 ± 0.32
0.183MetTrp: 0.183 ± 0.086
1.142MetTyr: 1.142 ± 0.243
0.0MetXaa: 0.0 ± 0.0
Asn
4.384AsnAla: 4.384 ± 0.541
0.457AsnCys: 0.457 ± 0.152
2.74AsnAsp: 2.74 ± 0.344
3.334AsnGlu: 3.334 ± 0.413
1.598AsnPhe: 1.598 ± 0.287
3.654AsnGly: 3.654 ± 0.436
1.324AsnHis: 1.324 ± 0.227
2.969AsnIle: 2.969 ± 0.412
3.745AsnLys: 3.745 ± 0.37
5.298AsnLeu: 5.298 ± 0.476
1.827AsnMet: 1.827 ± 0.224
3.38AsnAsn: 3.38 ± 0.444
3.288AsnPro: 3.288 ± 0.561
2.603AsnGln: 2.603 ± 0.33
2.74AsnArg: 2.74 ± 0.279
2.649AsnSer: 2.649 ± 0.296
3.654AsnThr: 3.654 ± 0.515
3.151AsnVal: 3.151 ± 0.411
0.685AsnTrp: 0.685 ± 0.166
2.421AsnTyr: 2.421 ± 0.377
0.0AsnXaa: 0.0 ± 0.0
Pro
3.197ProAla: 3.197 ± 0.382
0.228ProCys: 0.228 ± 0.111
2.832ProAsp: 2.832 ± 0.374
3.882ProGlu: 3.882 ± 0.432
1.598ProPhe: 1.598 ± 0.343
2.421ProGly: 2.421 ± 0.415
0.457ProHis: 0.457 ± 0.134
2.421ProIle: 2.421 ± 0.278
2.558ProLys: 2.558 ± 0.366
2.877ProLeu: 2.877 ± 0.387
1.005ProMet: 1.005 ± 0.176
2.512ProAsn: 2.512 ± 0.352
1.05ProPro: 1.05 ± 0.199
1.416ProGln: 1.416 ± 0.196
1.233ProArg: 1.233 ± 0.27
2.512ProSer: 2.512 ± 0.359
3.243ProThr: 3.243 ± 0.384
4.065ProVal: 4.065 ± 0.461
0.594ProTrp: 0.594 ± 0.232
1.096ProTyr: 1.096 ± 0.21
0.0ProXaa: 0.0 ± 0.0
Gln
4.567GlnAla: 4.567 ± 0.672
0.457GlnCys: 0.457 ± 0.162
1.872GlnAsp: 1.872 ± 0.289
3.836GlnGlu: 3.836 ± 0.455
1.416GlnPhe: 1.416 ± 0.224
2.329GlnGly: 2.329 ± 0.436
0.731GlnHis: 0.731 ± 0.134
2.786GlnIle: 2.786 ± 0.331
3.06GlnLys: 3.06 ± 0.475
3.928GlnLeu: 3.928 ± 0.446
1.37GlnMet: 1.37 ± 0.2
2.512GlnAsn: 2.512 ± 0.498
1.553GlnPro: 1.553 ± 0.291
1.69GlnGln: 1.69 ± 0.315
1.461GlnArg: 1.461 ± 0.315
2.466GlnSer: 2.466 ± 0.375
2.192GlnThr: 2.192 ± 0.286
3.425GlnVal: 3.425 ± 0.376
0.502GlnTrp: 0.502 ± 0.148
1.507GlnTyr: 1.507 ± 0.192
0.0GlnXaa: 0.0 ± 0.0
Arg
3.014ArgAla: 3.014 ± 0.363
0.457ArgCys: 0.457 ± 0.15
2.512ArgAsp: 2.512 ± 0.322
2.649ArgGlu: 2.649 ± 0.403
1.553ArgPhe: 1.553 ± 0.26
2.238ArgGly: 2.238 ± 0.309
0.594ArgHis: 0.594 ± 0.157
3.334ArgIle: 3.334 ± 0.365
3.151ArgLys: 3.151 ± 0.29
4.339ArgLeu: 4.339 ± 0.5
1.553ArgMet: 1.553 ± 0.274
3.197ArgAsn: 3.197 ± 0.487
1.324ArgPro: 1.324 ± 0.292
2.147ArgGln: 2.147 ± 0.319
2.375ArgArg: 2.375 ± 0.403
2.147ArgSer: 2.147 ± 0.328
2.329ArgThr: 2.329 ± 0.403
2.147ArgVal: 2.147 ± 0.364
0.548ArgTrp: 0.548 ± 0.164
1.69ArgTyr: 1.69 ± 0.272
0.0ArgXaa: 0.0 ± 0.0
Ser
4.065SerAla: 4.065 ± 0.425
0.502SerCys: 0.502 ± 0.159
2.466SerAsp: 2.466 ± 0.37
4.11SerGlu: 4.11 ± 0.39
2.009SerPhe: 2.009 ± 0.3
4.43SerGly: 4.43 ± 0.68
0.731SerHis: 0.731 ± 0.208
3.745SerIle: 3.745 ± 0.441
4.11SerLys: 4.11 ± 0.428
5.115SerLeu: 5.115 ± 0.53
1.69SerMet: 1.69 ± 0.33
2.74SerAsn: 2.74 ± 0.386
2.238SerPro: 2.238 ± 0.388
2.192SerGln: 2.192 ± 0.343
2.649SerArg: 2.649 ± 0.364
3.791SerSer: 3.791 ± 0.54
4.384SerThr: 4.384 ± 0.473
3.654SerVal: 3.654 ± 0.427
0.822SerTrp: 0.822 ± 0.216
2.192SerTyr: 2.192 ± 0.413
0.0SerXaa: 0.0 ± 0.0
Thr
5.389ThrAla: 5.389 ± 0.621
0.274ThrCys: 0.274 ± 0.137
4.11ThrAsp: 4.11 ± 0.313
4.065ThrGlu: 4.065 ± 0.495
2.832ThrPhe: 2.832 ± 0.405
5.161ThrGly: 5.161 ± 0.465
1.233ThrHis: 1.233 ± 0.255
3.562ThrIle: 3.562 ± 0.367
4.019ThrLys: 4.019 ± 0.508
5.48ThrLeu: 5.48 ± 0.488
1.598ThrMet: 1.598 ± 0.291
3.699ThrAsn: 3.699 ± 0.423
3.471ThrPro: 3.471 ± 0.398
2.969ThrGln: 2.969 ± 0.339
2.512ThrArg: 2.512 ± 0.305
3.654ThrSer: 3.654 ± 0.403
3.608ThrThr: 3.608 ± 0.415
5.572ThrVal: 5.572 ± 0.615
1.05ThrTrp: 1.05 ± 0.298
1.781ThrTyr: 1.781 ± 0.299
0.0ThrXaa: 0.0 ± 0.0
Val
6.531ValAla: 6.531 ± 0.822
0.685ValCys: 0.685 ± 0.224
3.562ValAsp: 3.562 ± 0.398
4.521ValGlu: 4.521 ± 0.497
2.009ValPhe: 2.009 ± 0.335
4.476ValGly: 4.476 ± 0.603
1.598ValHis: 1.598 ± 0.295
3.836ValIle: 3.836 ± 0.562
4.11ValLys: 4.11 ± 0.439
3.928ValLeu: 3.928 ± 0.442
2.421ValMet: 2.421 ± 0.327
4.339ValAsn: 4.339 ± 0.396
3.106ValPro: 3.106 ± 0.46
2.695ValGln: 2.695 ± 0.323
3.014ValArg: 3.014 ± 0.437
3.836ValSer: 3.836 ± 0.459
5.161ValThr: 5.161 ± 0.55
4.156ValVal: 4.156 ± 0.483
0.913ValTrp: 0.913 ± 0.194
2.466ValTyr: 2.466 ± 0.33
0.0ValXaa: 0.0 ± 0.0
Trp
0.913TrpAla: 0.913 ± 0.219
0.091TrpCys: 0.091 ± 0.065
1.096TrpAsp: 1.096 ± 0.247
0.685TrpGlu: 0.685 ± 0.245
0.731TrpPhe: 0.731 ± 0.213
0.776TrpGly: 0.776 ± 0.247
0.365TrpHis: 0.365 ± 0.132
1.005TrpIle: 1.005 ± 0.212
0.822TrpLys: 0.822 ± 0.224
1.05TrpLeu: 1.05 ± 0.275
0.183TrpMet: 0.183 ± 0.101
0.822TrpAsn: 0.822 ± 0.207
0.228TrpPro: 0.228 ± 0.091
0.731TrpGln: 0.731 ± 0.146
0.731TrpArg: 0.731 ± 0.215
0.548TrpSer: 0.548 ± 0.134
0.548TrpThr: 0.548 ± 0.173
1.05TrpVal: 1.05 ± 0.263
0.32TrpTrp: 0.32 ± 0.188
0.411TrpTyr: 0.411 ± 0.161
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.832TyrAla: 2.832 ± 0.365
0.594TyrCys: 0.594 ± 0.187
2.786TyrAsp: 2.786 ± 0.327
1.964TyrGlu: 1.964 ± 0.333
1.187TyrPhe: 1.187 ± 0.271
2.74TyrGly: 2.74 ± 0.495
0.913TyrHis: 0.913 ± 0.212
2.375TyrIle: 2.375 ± 0.46
2.009TyrLys: 2.009 ± 0.38
2.649TyrLeu: 2.649 ± 0.382
0.868TyrMet: 0.868 ± 0.205
2.284TyrAsn: 2.284 ± 0.25
2.009TyrPro: 2.009 ± 0.404
2.009TyrGln: 2.009 ± 0.385
1.416TyrArg: 1.416 ± 0.341
2.055TyrSer: 2.055 ± 0.311
1.827TyrThr: 1.827 ± 0.263
2.466TyrVal: 2.466 ± 0.344
0.594TyrTrp: 0.594 ± 0.161
1.324TyrTyr: 1.324 ± 0.289
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 74 proteins (21897 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski