Escherichia virus Jk06
Average proteome isoelectric point is 6.74
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 82 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q45PW9|Q45PW9_9CAUD JK_47P OS=Escherichia virus Jk06 OX=1920998 GN=JK_47 PE=4 SV=1
MM1 pKa = 7.33 KK2 pKa = 10.38 AIIINNEE9 pKa = 2.81 IRR11 pKa = 11.84 YY12 pKa = 9.99 DD13 pKa = 4.01 EE14 pKa = 5.36 DD15 pKa = 4.13 SMSLAEE21 pKa = 4.91 LGYY24 pKa = 10.56 QIGEE28 pKa = 4.03 EE29 pKa = 4.26 VEE31 pKa = 4.27 VYY33 pKa = 10.14 EE34 pKa = 4.9 IEE36 pKa = 4.43 GFYY39 pKa = 10.58 MMSLKK44 pKa = 10.84 DD45 pKa = 3.5 HH46 pKa = 6.56 VYY48 pKa = 10.84 VDD50 pKa = 3.43 GTLAIFQGEE59 pKa = 4.15 QFVVSNEE66 pKa = 4.09 EE67 pKa = 3.88 IKK69 pKa = 11.1 VVAA72 pKa = 4.56
Molecular weight: 8.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.852
IPC2_protein 4.101
IPC_protein 3.961
Toseland 3.808
ProMoST 4.075
Dawson 3.897
Bjellqvist 4.062
Wikipedia 3.77
Rodwell 3.795
Grimsley 3.732
Solomon 3.884
Lehninger 3.834
Nozaki 4.037
DTASelect 4.113
Thurlkill 3.834
EMBOSS 3.795
Sillero 4.062
Patrickios 1.888
IPC_peptide 3.897
IPC2_peptide 4.05
IPC2.peptide.svr19 3.963
Protein with the highest isoelectric point:
>tr|Q45PX8|Q45PX8_9CAUD JK_38P OS=Escherichia virus Jk06 OX=1920998 GN=JK_38 PE=4 SV=1
MM1 pKa = 7.08 KK2 pKa = 9.74 TLVWEE7 pKa = 4.39 EE8 pKa = 3.71 MTPAEE13 pKa = 4.17 KK14 pKa = 10.56 LAVKK18 pKa = 10.24 ALSTHH23 pKa = 5.25 SFEE26 pKa = 4.52 AFLRR30 pKa = 11.84 VWFQLSQGEE39 pKa = 4.17 RR40 pKa = 11.84 YY41 pKa = 9.13 IPNWHH46 pKa = 6.78 HH47 pKa = 7.09 KK48 pKa = 7.75 YY49 pKa = 9.91 LCRR52 pKa = 11.84 IIDD55 pKa = 3.65 EE56 pKa = 4.72 VISGEE61 pKa = 4.42 RR62 pKa = 11.84 RR63 pKa = 11.84 DD64 pKa = 3.98 TIINVAPGAGKK75 pKa = 8.19 TEE77 pKa = 3.77 ITSIHH82 pKa = 5.92 FPVYY86 pKa = 11.26 SMLKK90 pKa = 9.37 LKK92 pKa = 10.38 KK93 pKa = 10.06 VRR95 pKa = 11.84 NLSLSFSDD103 pKa = 4.35 SLVKK107 pKa = 10.62 RR108 pKa = 11.84 NSKK111 pKa = 9.98 RR112 pKa = 11.84 VRR114 pKa = 11.84 DD115 pKa = 4.13 LIKK118 pKa = 10.55 SVSFRR123 pKa = 11.84 NYY125 pKa = 10.16 GPVSSARR132 pKa = 11.84 ARR134 pKa = 11.84 MTRR137 pKa = 11.84 FRR139 pKa = 11.84 FLTRR143 pKa = 11.84 VEE145 pKa = 3.93 RR146 pKa = 11.84 LGSKK150 pKa = 10.2 RR151 pKa = 11.84 YY152 pKa = 9.61 LRR154 pKa = 11.84 QLAVRR159 pKa = 11.84 LPALVAVILLKK170 pKa = 10.67 PIAVAA175 pKa = 4.34
Molecular weight: 20.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.316
IPC2_protein 9.911
IPC_protein 10.847
Toseland 11.038
ProMoST 10.877
Dawson 11.096
Bjellqvist 10.847
Wikipedia 11.345
Rodwell 11.301
Grimsley 11.14
Solomon 11.301
Lehninger 11.257
Nozaki 11.008
DTASelect 10.847
Thurlkill 11.023
EMBOSS 11.462
Sillero 11.038
Patrickios 11.008
IPC_peptide 11.301
IPC2_peptide 9.838
IPC2.peptide.svr19 8.48
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
82
0
82
14581
72
1141
177.8
19.77
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.079 ± 0.371
1.536 ± 0.173
5.596 ± 0.252
5.74 ± 0.325
3.985 ± 0.172
6.913 ± 0.329
1.598 ± 0.164
6.392 ± 0.161
5.706 ± 0.366
8.497 ± 0.353
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.592 ± 0.185
5.013 ± 0.257
3.319 ± 0.213
3.854 ± 0.21
5.473 ± 0.252
7.976 ± 0.362
5.974 ± 0.265
7.091 ± 0.269
1.125 ± 0.089
3.539 ± 0.185
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here