Tepidimonas fonticaldi

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiales genera incertae sedis; Tepidimonas

Average proteome isoelectric point is 7.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2430 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1A6DY84|A0A1A6DY84_9BURK PTS fructose transporter subunit IIA OS=Tepidimonas fonticaldi OX=1101373 GN=A9O67_12235 PE=4 SV=1
MM1 pKa = 7.34SALAEE6 pKa = 4.21TVATPEE12 pKa = 4.21TEE14 pKa = 4.02MPAPLVFTDD23 pKa = 3.92AAAAKK28 pKa = 9.24VAEE31 pKa = 5.03LIAEE35 pKa = 4.35EE36 pKa = 4.62GNPDD40 pKa = 2.99LKK42 pKa = 11.13LRR44 pKa = 11.84VFVQGGGCSGFQYY57 pKa = 10.9GFTFDD62 pKa = 4.72EE63 pKa = 5.17VINDD67 pKa = 5.27DD68 pKa = 3.86DD69 pKa = 3.93TQMTKK74 pKa = 10.88NGVTLLIDD82 pKa = 3.64AMSYY86 pKa = 10.32QYY88 pKa = 11.5LVGAEE93 pKa = 3.79IDD95 pKa = 3.91YY96 pKa = 11.36KK97 pKa = 11.19EE98 pKa = 4.67DD99 pKa = 3.23LQGAQFVIRR108 pKa = 11.84NPNATTTCGCGSSFSVGG125 pKa = 3.1

Molecular weight: 13.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1A6DX55|A0A1A6DX55_9BURK Oxidoreductase OS=Tepidimonas fonticaldi OX=1101373 GN=A9O67_01740 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.07QPSKK9 pKa = 9.07IRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.91GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2430

0

2430

812492

27

1911

334.4

36.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.058 ± 0.08

0.891 ± 0.016

5.41 ± 0.039

5.338 ± 0.041

3.082 ± 0.028

8.413 ± 0.039

2.462 ± 0.025

3.923 ± 0.039

2.372 ± 0.044

11.137 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.154 ± 0.022

1.994 ± 0.026

5.892 ± 0.045

4.01 ± 0.033

8.183 ± 0.052

3.993 ± 0.03

4.889 ± 0.033

7.938 ± 0.042

1.795 ± 0.028

2.064 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski