Dragonfly associated gemykibivirus 1

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemykibivirus; Gemykibivirus draga1

Average proteome isoelectric point is 7.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K0A2I9|K0A2I9_9VIRU Putative coat protein OS=Dragonfly associated gemykibivirus 1 OX=1234887 PE=4 SV=1
MM1 pKa = 7.97PFRR4 pKa = 11.84FAAKK8 pKa = 10.01YY9 pKa = 10.66GLLTYY14 pKa = 7.2PQCGDD19 pKa = 3.72LDD21 pKa = 3.69PWAVNDD27 pKa = 3.42MLGRR31 pKa = 11.84LGAEE35 pKa = 3.67CVIGRR40 pKa = 11.84EE41 pKa = 3.93NHH43 pKa = 4.93QAGGVHH49 pKa = 5.9LHH51 pKa = 6.31AFFMFEE57 pKa = 4.26RR58 pKa = 11.84KK59 pKa = 9.22FEE61 pKa = 4.24SRR63 pKa = 11.84NVRR66 pKa = 11.84VFDD69 pKa = 4.3VDD71 pKa = 3.32GHH73 pKa = 6.4HH74 pKa = 6.91PNIVRR79 pKa = 11.84GYY81 pKa = 7.86STPEE85 pKa = 4.31DD86 pKa = 3.02GWAYY90 pKa = 8.57ATKK93 pKa = 10.69DD94 pKa = 3.23GDD96 pKa = 4.11VVAGALEE103 pKa = 4.38CPRR106 pKa = 11.84PRR108 pKa = 11.84TEE110 pKa = 3.87VSEE113 pKa = 4.92SSSKK117 pKa = 8.44WARR120 pKa = 11.84AILAEE125 pKa = 3.97TRR127 pKa = 11.84EE128 pKa = 4.06EE129 pKa = 3.94FFAIVAEE136 pKa = 4.23LDD138 pKa = 3.39PRR140 pKa = 11.84ALCVSFGSLRR150 pKa = 11.84AYY152 pKa = 10.47ADD154 pKa = 2.97WKK156 pKa = 10.32YY157 pKa = 10.99RR158 pKa = 11.84PARR161 pKa = 11.84DD162 pKa = 3.64PYY164 pKa = 9.12ITPEE168 pKa = 4.71GISFDD173 pKa = 3.6TSEE176 pKa = 5.23LPEE179 pKa = 5.82LDD181 pKa = 4.04LWVQQTLSGFTGRR194 pKa = 11.84PKK196 pKa = 10.8SLILIGDD203 pKa = 3.6TRR205 pKa = 11.84LGKK208 pKa = 7.79TLWARR213 pKa = 11.84SLGRR217 pKa = 11.84HH218 pKa = 5.87AYY220 pKa = 10.17FGGLFCLDD228 pKa = 3.79EE229 pKa = 4.48SLEE232 pKa = 4.3DD233 pKa = 2.93VDD235 pKa = 5.06YY236 pKa = 11.57AVFDD240 pKa = 4.16DD241 pKa = 4.05MQGGLEE247 pKa = 4.15FFHH250 pKa = 7.45AYY252 pKa = 9.59KK253 pKa = 10.47FWLGAQKK260 pKa = 10.36QFYY263 pKa = 8.95ATDD266 pKa = 3.58KK267 pKa = 11.0YY268 pKa = 10.74RR269 pKa = 11.84GKK271 pKa = 10.32QLVNWGRR278 pKa = 11.84PSIYY282 pKa = 9.87ISNTNPLADD291 pKa = 4.19KK292 pKa = 10.9GADD295 pKa = 3.53VDD297 pKa = 4.03WLMGNCVIVHH307 pKa = 5.5VDD309 pKa = 3.41RR310 pKa = 11.84PIFRR314 pKa = 11.84ASTEE318 pKa = 3.81

Molecular weight: 35.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K0A2I9|K0A2I9_9VIRU Putative coat protein OS=Dragonfly associated gemykibivirus 1 OX=1234887 PE=4 SV=1
MM1 pKa = 7.2YY2 pKa = 9.74RR3 pKa = 11.84RR4 pKa = 11.84KK5 pKa = 8.38YY6 pKa = 8.52TRR8 pKa = 11.84TIRR11 pKa = 11.84RR12 pKa = 11.84GRR14 pKa = 11.84AGSSKK19 pKa = 10.13KK20 pKa = 9.43RR21 pKa = 11.84RR22 pKa = 11.84SYY24 pKa = 10.11VGRR27 pKa = 11.84RR28 pKa = 11.84LSSRR32 pKa = 11.84RR33 pKa = 11.84TTKK36 pKa = 10.16TVMKK40 pKa = 8.45RR41 pKa = 11.84TSRR44 pKa = 11.84RR45 pKa = 11.84RR46 pKa = 11.84ILNISSQKK54 pKa = 9.96KK55 pKa = 9.38RR56 pKa = 11.84NNAQPVNFNYY66 pKa = 10.42LGEE69 pKa = 4.27SPIPGSKK76 pKa = 10.76VMGSDD81 pKa = 2.77RR82 pKa = 11.84TTYY85 pKa = 10.94LLWRR89 pKa = 11.84PTALTDD95 pKa = 3.5DD96 pKa = 3.69TDD98 pKa = 3.9IYY100 pKa = 11.37SSASRR105 pKa = 11.84GAQQIYY111 pKa = 8.36WRR113 pKa = 11.84GIKK116 pKa = 9.4EE117 pKa = 3.79RR118 pKa = 11.84AEE120 pKa = 4.15VGTSSGAAWRR130 pKa = 11.84WRR132 pKa = 11.84RR133 pKa = 11.84IVFEE137 pKa = 4.47AKK139 pKa = 9.99SLNINGLISNVEE151 pKa = 3.87TSAGFNRR158 pKa = 11.84AMVEE162 pKa = 3.99LSGDD166 pKa = 3.42PPANLRR172 pKa = 11.84NSLEE176 pKa = 4.0SFLFQGSVGVDD187 pKa = 2.72WNTVFNAKK195 pKa = 9.37VDD197 pKa = 3.91NNRR200 pKa = 11.84CRR202 pKa = 11.84LLSDD206 pKa = 4.03KK207 pKa = 10.46VRR209 pKa = 11.84HH210 pKa = 5.57LQSGNDD216 pKa = 2.93RR217 pKa = 11.84GRR219 pKa = 11.84FYY221 pKa = 10.72TFRR224 pKa = 11.84QWIPLNKK231 pKa = 9.4TMVYY235 pKa = 10.81NDD237 pKa = 4.93DD238 pKa = 3.72EE239 pKa = 4.94VGDD242 pKa = 4.35DD243 pKa = 3.61KK244 pKa = 11.55QSNTFASPGRR254 pKa = 11.84KK255 pKa = 9.65GMGDD259 pKa = 3.38VYY261 pKa = 10.93VYY263 pKa = 11.67DD264 pKa = 4.12MFQCLTSDD272 pKa = 5.6AINTLNFNPQATLYY286 pKa = 8.14WHH288 pKa = 6.53EE289 pKa = 4.17RR290 pKa = 3.47

Molecular weight: 33.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

934

77

318

233.5

26.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.03 ± 1.07

1.606 ± 0.401

6.317 ± 0.528

4.925 ± 0.854

5.139 ± 0.606

8.458 ± 0.498

2.034 ± 0.543

3.747 ± 0.32

4.069 ± 0.644

7.602 ± 0.552

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.034 ± 0.212

4.069 ± 1.485

5.139 ± 1.094

3.212 ± 0.341

8.672 ± 1.349

7.066 ± 1.085

5.139 ± 0.697

6.103 ± 0.11

2.57 ± 0.219

4.069 ± 0.165
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski