Streptomyces phage Braelyn

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Samistivirus; unclassified Samistivirus

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 206 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514U210|A0A514U210_9CAUD FtsK-like DNA translocase OS=Streptomyces phage Braelyn OX=2593356 GN=106 PE=4 SV=1
MM1 pKa = 7.93AYY3 pKa = 10.52YY4 pKa = 9.88PINQFFYY11 pKa = 10.93DD12 pKa = 3.93SEE14 pKa = 4.61TNNIIVQFAVYY25 pKa = 8.87TEE27 pKa = 4.18KK28 pKa = 10.3TYY30 pKa = 9.57EE31 pKa = 4.04TAEE34 pKa = 3.9MDD36 pKa = 3.68DD37 pKa = 4.44PYY39 pKa = 11.77DD40 pKa = 3.91VIEE43 pKa = 5.37AEE45 pKa = 4.47LFLDD49 pKa = 3.7AGKK52 pKa = 10.55FKK54 pKa = 10.5IGEE57 pKa = 4.07YY58 pKa = 10.26RR59 pKa = 11.84PAALDD64 pKa = 3.55GSVTQEE70 pKa = 4.05FIGRR74 pKa = 11.84DD75 pKa = 3.31LGQAPSVTPPNPVPEE90 pKa = 4.46EE91 pKa = 3.88PAA93 pKa = 4.07

Molecular weight: 10.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514U1Y8|A0A514U1Y8_9CAUD Hydrolase OS=Streptomyces phage Braelyn OX=2593356 GN=27 PE=4 SV=1
MM1 pKa = 7.74ALTGNKK7 pKa = 8.3GRR9 pKa = 11.84CWFQRR14 pKa = 11.84CSRR17 pKa = 11.84KK18 pKa = 8.66ATTTIRR24 pKa = 11.84YY25 pKa = 7.5YY26 pKa = 10.01NKK28 pKa = 10.41SVVVCDD34 pKa = 3.15GHH36 pKa = 7.32KK37 pKa = 10.88NLDD40 pKa = 3.75GVGCPSSRR48 pKa = 11.84RR49 pKa = 11.84RR50 pKa = 11.84PKK52 pKa = 10.46SS53 pKa = 3.11

Molecular weight: 5.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

206

0

206

34223

37

2103

166.1

18.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.351 ± 0.333

1.014 ± 0.131

6.481 ± 0.16

7.115 ± 0.374

3.813 ± 0.129

7.445 ± 0.221

1.943 ± 0.13

5.292 ± 0.136

6.469 ± 0.308

7.29 ± 0.179

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.843 ± 0.126

4.409 ± 0.159

3.734 ± 0.156

3.302 ± 0.187

5.651 ± 0.229

5.923 ± 0.247

6.031 ± 0.399

7.285 ± 0.223

1.905 ± 0.083

3.702 ± 0.17
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski