Streptomyces phage Alderaan

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Austintatiousvirus; unclassified Austintatiousvirus

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G4AVP3|A0A7G4AVP3_9CAUD Terminase OS=Streptomyces phage Alderaan OX=2739833 GN=HQ601_00010 PE=4 SV=1
MM1 pKa = 8.0PSTPDD6 pKa = 3.19QRR8 pKa = 11.84CTITLPVTLTVAGCSATVGTLTVDD32 pKa = 3.23AAEE35 pKa = 4.0QLGPQLADD43 pKa = 3.86FLDD46 pKa = 4.53DD47 pKa = 3.86LAAAYY52 pKa = 8.15RR53 pKa = 11.84ATVGPLDD60 pKa = 5.2DD61 pKa = 3.94EE62 pKa = 4.57HH63 pKa = 8.91QEE65 pKa = 3.96VSPDD69 pKa = 3.33GTSS72 pKa = 2.7

Molecular weight: 7.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G4AVQ5|A0A7G4AVQ5_9CAUD Uncharacterized protein OS=Streptomyces phage Alderaan OX=2739833 GN=HQ601_00048 PE=4 SV=1
MM1 pKa = 6.86NTPEE5 pKa = 3.94RR6 pKa = 11.84FAAKK10 pKa = 9.65VDD12 pKa = 3.59PAGPVSLYY20 pKa = 10.45RR21 pKa = 11.84DD22 pKa = 3.54APGPCHH28 pKa = 7.32LWTGGARR35 pKa = 11.84SKK37 pKa = 10.65RR38 pKa = 11.84PHH40 pKa = 6.89DD41 pKa = 4.26AGEE44 pKa = 4.03HH45 pKa = 5.75GEE47 pKa = 4.09FYY49 pKa = 11.11GAFKK53 pKa = 11.16ANGRR57 pKa = 11.84TVRR60 pKa = 11.84AHH62 pKa = 5.31QYY64 pKa = 10.54AYY66 pKa = 9.5EE67 pKa = 4.14QARR70 pKa = 11.84GPIPAGAEE78 pKa = 3.57VDD80 pKa = 3.49HH81 pKa = 6.86RR82 pKa = 11.84CRR84 pKa = 11.84RR85 pKa = 11.84RR86 pKa = 11.84NCVNVAHH93 pKa = 7.3LEE95 pKa = 4.33LTDD98 pKa = 3.55HH99 pKa = 6.44RR100 pKa = 11.84TNTLRR105 pKa = 11.84SNGPTAINARR115 pKa = 11.84KK116 pKa = 8.66KK117 pKa = 9.25ACHH120 pKa = 6.51RR121 pKa = 11.84GHH123 pKa = 7.18PFDD126 pKa = 3.77ATNTYY131 pKa = 9.53VRR133 pKa = 11.84PTGARR138 pKa = 11.84ACRR141 pKa = 11.84ACRR144 pKa = 11.84RR145 pKa = 11.84PTTEE149 pKa = 4.91RR150 pKa = 11.84IAAA153 pKa = 3.9

Molecular weight: 16.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

10461

48

805

209.2

22.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

15.553 ± 0.57

0.593 ± 0.117

6.73 ± 0.336

5.86 ± 0.303

1.902 ± 0.154

8.403 ± 0.545

1.979 ± 0.193

3.308 ± 0.254

2.046 ± 0.207

8.288 ± 0.396

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.902 ± 0.198

2.065 ± 0.148

6.233 ± 0.394

4.13 ± 0.327

9.005 ± 0.536

4.588 ± 0.301

7.303 ± 0.352

6.338 ± 0.352

1.797 ± 0.219

1.979 ± 0.165
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski