Streptomyces sp. AA4
Average proteome isoelectric point is 6.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8394 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D9UXG6|D9UXG6_9ACTN Tryptophan--tRNA ligase OS=Streptomyces sp. AA4 OX=591158 GN=SSMG_06442 PE=3 SV=1
MM1 pKa = 7.58 SGTRR5 pKa = 11.84 PQHH8 pKa = 6.84 PNDD11 pKa = 2.95 AWYY14 pKa = 9.99 GGTMAITLDD23 pKa = 3.61 AQLLEE28 pKa = 4.38 ILACPSPDD36 pKa = 3.39 HH37 pKa = 6.95 APLRR41 pKa = 11.84 PGTPDD46 pKa = 3.56 DD47 pKa = 4.71 AEE49 pKa = 5.91 ADD51 pKa = 3.36 ALTCTEE57 pKa = 4.44 CGRR60 pKa = 11.84 VYY62 pKa = 10.34 PVRR65 pKa = 11.84 DD66 pKa = 4.78 GIPVLLLDD74 pKa = 4.22 EE75 pKa = 4.57 ATLPGTAGPDD85 pKa = 3.4 ADD87 pKa = 4.93 HH88 pKa = 7.24 GDD90 pKa = 3.86 SAA92 pKa = 5.04
Molecular weight: 9.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.78
IPC2_protein 3.999
IPC_protein 3.948
Toseland 3.732
ProMoST 4.139
Dawson 3.961
Bjellqvist 4.113
Wikipedia 3.923
Rodwell 3.783
Grimsley 3.643
Solomon 3.948
Lehninger 3.897
Nozaki 4.088
DTASelect 4.342
Thurlkill 3.808
EMBOSS 3.935
Sillero 4.075
Patrickios 1.952
IPC_peptide 3.935
IPC2_peptide 4.05
IPC2.peptide.svr19 3.96
Protein with the highest isoelectric point:
>tr|D9VIX6|D9VIX6_9ACTN HTH cro/C1-type domain-containing protein OS=Streptomyces sp. AA4 OX=591158 GN=SSMG_08157 PE=4 SV=1
MM1 pKa = 7.94 AGMGLDD7 pKa = 3.15 RR8 pKa = 11.84 GLHH11 pKa = 5.22 RR12 pKa = 11.84 RR13 pKa = 11.84 ASRR16 pKa = 11.84 GPRR19 pKa = 11.84 HH20 pKa = 6.04 RR21 pKa = 11.84 PSRR24 pKa = 11.84 PQEE27 pKa = 4.11 DD28 pKa = 3.11 PGARR32 pKa = 11.84 GRR34 pKa = 11.84 GTRR37 pKa = 11.84 RR38 pKa = 11.84 RR39 pKa = 11.84 PGRR42 pKa = 11.84 RR43 pKa = 11.84 EE44 pKa = 3.57 TLRR47 pKa = 11.84 PRR49 pKa = 11.84 SQARR53 pKa = 11.84 VQARR57 pKa = 11.84 RR58 pKa = 11.84 LRR60 pKa = 11.84 EE61 pKa = 3.57 GSRR64 pKa = 11.84 PDD66 pKa = 2.78 RR67 pKa = 11.84 HH68 pKa = 5.78 GRR70 pKa = 11.84 NRR72 pKa = 11.84 LPRR75 pKa = 11.84 PAARR79 pKa = 11.84 VAAAPAAGRR88 pKa = 11.84 GRR90 pKa = 11.84 PRR92 pKa = 11.84 PPRR95 pKa = 11.84 GSRR98 pKa = 11.84 TRR100 pKa = 11.84 RR101 pKa = 11.84 RR102 pKa = 11.84 RR103 pKa = 11.84 PRR105 pKa = 11.84 RR106 pKa = 11.84 ARR108 pKa = 11.84 RR109 pKa = 11.84 ARR111 pKa = 11.84 RR112 pKa = 11.84 RR113 pKa = 11.84 SRR115 pKa = 11.84 DD116 pKa = 3.5 RR117 pKa = 11.84 GPGAADD123 pKa = 3.52 PPHH126 pKa = 6.78 RR127 pKa = 11.84 RR128 pKa = 11.84 ARR130 pKa = 11.84 PARR133 pKa = 11.84 RR134 pKa = 11.84 GQRR137 pKa = 11.84 RR138 pKa = 11.84 RR139 pKa = 11.84 RR140 pKa = 11.84 GLVRR144 pKa = 11.84 PAARR148 pKa = 11.84 RR149 pKa = 11.84 ARR151 pKa = 11.84 CGAGVPAQPSRR162 pKa = 11.84 RR163 pKa = 11.84 PGAGRR168 pKa = 11.84 PRR170 pKa = 11.84 PGPRR174 pKa = 11.84 RR175 pKa = 11.84 RR176 pKa = 11.84 RR177 pKa = 11.84 PRR179 pKa = 11.84 EE180 pKa = 3.33 ADD182 pKa = 2.88 HH183 pKa = 7.27 RR184 pKa = 11.84 IRR186 pKa = 11.84 PGPLGRR192 pKa = 11.84 PLLAPARR199 pKa = 11.84 RR200 pKa = 11.84 RR201 pKa = 11.84 IRR203 pKa = 11.84 RR204 pKa = 11.84 AGPDD208 pKa = 2.89 RR209 pKa = 11.84 RR210 pKa = 11.84 RR211 pKa = 11.84 GRR213 pKa = 11.84 DD214 pKa = 3.32 PRR216 pKa = 11.84 EE217 pKa = 3.77 DD218 pKa = 3.74 RR219 pKa = 11.84 EE220 pKa = 4.16 PGRR223 pKa = 11.84 PVPAPDD229 pKa = 2.91 RR230 pKa = 11.84 VRR232 pKa = 11.84 RR233 pKa = 11.84 PRR235 pKa = 11.84 RR236 pKa = 11.84 ARR238 pKa = 11.84 PDD240 pKa = 3.29 RR241 pKa = 11.84 GIHH244 pKa = 5.93 RR245 pKa = 11.84 PHH247 pKa = 6.69 AALRR251 pKa = 11.84 RR252 pKa = 11.84 HH253 pKa = 5.58 PRR255 pKa = 11.84 TRR257 pKa = 11.84 RR258 pKa = 11.84 ADD260 pKa = 3.53 PQIPQHH266 pKa = 5.4 PQHH269 pKa = 6.64 RR270 pKa = 11.84 PVRR273 pKa = 11.84 QEE275 pKa = 3.31 PAAAGAARR283 pKa = 11.84 AARR286 pKa = 11.84 SRR288 pKa = 11.84 RR289 pKa = 11.84 ARR291 pKa = 11.84 QPGVRR296 pKa = 11.84 GGPVRR301 pKa = 11.84 RR302 pKa = 11.84 GRR304 pKa = 11.84 ADD306 pKa = 3.39 GPRR309 pKa = 11.84 PAGAADD315 pKa = 3.88 DD316 pKa = 4.13 VPRR319 pKa = 11.84 DD320 pKa = 3.44 LRR322 pKa = 11.84 DD323 pKa = 3.27 RR324 pKa = 11.84 ADD326 pKa = 3.74 RR327 pKa = 11.84 QPPRR331 pKa = 11.84 RR332 pKa = 11.84 GRR334 pKa = 11.84 RR335 pKa = 11.84 AGAGRR340 pKa = 11.84 AAPRR344 pKa = 11.84 VRR346 pKa = 11.84 RR347 pKa = 11.84 RR348 pKa = 11.84 QRR350 pKa = 11.84 RR351 pKa = 11.84 RR352 pKa = 11.84 RR353 pKa = 11.84 AHH355 pKa = 5.74 GARR358 pKa = 11.84 RR359 pKa = 11.84 ADD361 pKa = 3.81 RR362 pKa = 11.84 DD363 pKa = 3.47 GARR366 pKa = 11.84 ALPACGRR373 pKa = 11.84 AGPARR378 pKa = 11.84 GPRR381 pKa = 11.84 PRR383 pKa = 11.84 RR384 pKa = 11.84 VGRR387 pKa = 11.84 ASRR390 pKa = 11.84 ARR392 pKa = 11.84 RR393 pKa = 11.84 PGRR396 pKa = 11.84 GVPGFPADD404 pKa = 3.76 PLGHH408 pKa = 6.55 GGAGRR413 pKa = 11.84 PAHH416 pKa = 5.71 RR417 pKa = 11.84 AVRR420 pKa = 11.84 RR421 pKa = 11.84 PAGRR425 pKa = 11.84 GLRR428 pKa = 11.84 SRR430 pKa = 11.84 SQHH433 pKa = 4.28 QGRR436 pKa = 11.84 TRR438 pKa = 11.84 RR439 pKa = 11.84 RR440 pKa = 11.84 RR441 pKa = 11.84 GAGPGRR447 pKa = 11.84 ARR449 pKa = 11.84 ADD451 pKa = 3.33 RR452 pKa = 11.84 RR453 pKa = 11.84 ARR455 pKa = 11.84 SGPGRR460 pKa = 11.84 PPAHH464 pKa = 7.47 RR465 pKa = 11.84 SRR467 pKa = 11.84 PPGRR471 pKa = 11.84 VRR473 pKa = 11.84 RR474 pKa = 11.84 RR475 pKa = 11.84 RR476 pKa = 11.84 PPGSRR481 pKa = 11.84 PHH483 pKa = 6.16 TPAPARR489 pKa = 11.84 GGRR492 pKa = 11.84 RR493 pKa = 11.84 ACFRR497 pKa = 11.84 RR498 pKa = 11.84 VRR500 pKa = 11.84 SGGPRR505 pKa = 11.84 RR506 pKa = 11.84 GRR508 pKa = 11.84 GPRR511 pKa = 11.84 RR512 pKa = 11.84 PRR514 pKa = 11.84 RR515 pKa = 11.84 PGRR518 pKa = 11.84 EE519 pKa = 3.91 HH520 pKa = 6.9 RR521 pKa = 11.84 CRR523 pKa = 11.84 PAGQSGRR530 pKa = 11.84 PRR532 pKa = 11.84 PGHH535 pKa = 6.7 PGSAPARR542 pKa = 11.84 RR543 pKa = 11.84 GHH545 pKa = 5.59 RR546 pKa = 11.84 HH547 pKa = 4.96 GRR549 pKa = 11.84 PPGARR554 pKa = 11.84 RR555 pKa = 11.84 RR556 pKa = 11.84 RR557 pKa = 11.84 AVAARR562 pKa = 11.84 PGRR565 pKa = 11.84 PATRR569 pKa = 11.84 RR570 pKa = 11.84 ADD572 pKa = 3.44 AAVPAGPHH580 pKa = 5.22 RR581 pKa = 11.84 EE582 pKa = 3.54 RR583 pKa = 11.84 RR584 pKa = 11.84 GRR586 pKa = 11.84 LRR588 pKa = 11.84 RR589 pKa = 11.84 ARR591 pKa = 11.84 RR592 pKa = 11.84 LGHH595 pKa = 6.94 RR596 pKa = 11.84 GRR598 pKa = 11.84 AGPPDD603 pKa = 3.46 RR604 pKa = 11.84 VRR606 pKa = 11.84 RR607 pKa = 11.84 PRR609 pKa = 11.84 SPGPAAASRR618 pKa = 11.84 PPRR621 pKa = 11.84 SDD623 pKa = 2.72 RR624 pKa = 11.84 AGRR627 pKa = 11.84 RR628 pKa = 11.84 TRR630 pKa = 11.84 PARR633 pKa = 11.84 PRR635 pKa = 11.84 DD636 pKa = 3.59 PRR638 pKa = 11.84 QGRR641 pKa = 11.84 HH642 pKa = 4.34 RR643 pKa = 11.84 TAARR647 pKa = 11.84 PRR649 pKa = 11.84 PDD651 pKa = 2.95 RR652 pKa = 11.84 RR653 pKa = 11.84 RR654 pKa = 11.84 VRR656 pKa = 11.84 AARR659 pKa = 11.84 AAARR663 pKa = 11.84 RR664 pKa = 11.84 AAGAALRR671 pKa = 11.84 GPDD674 pKa = 3.02 VPLRR678 pKa = 11.84 HH679 pKa = 6.21 RR680 pKa = 11.84 PRR682 pKa = 11.84 RR683 pKa = 11.84 DD684 pKa = 2.93 LHH686 pKa = 6.49 RR687 pKa = 11.84 DD688 pKa = 2.99 RR689 pKa = 11.84 TRR691 pKa = 11.84 GVEE694 pKa = 3.91 VGRR697 pKa = 11.84 RR698 pKa = 11.84 AALGRR703 pKa = 11.84 PRR705 pKa = 11.84 PSVCGRR711 pKa = 11.84 DD712 pKa = 3.33 PARR715 pKa = 11.84 RR716 pKa = 11.84 RR717 pKa = 11.84 RR718 pKa = 11.84 PGPRR722 pKa = 11.84 AGAGPDD728 pKa = 3.08 RR729 pKa = 11.84 HH730 pKa = 5.63 GTGPRR735 pKa = 11.84 DD736 pKa = 3.32 AGARR740 pKa = 11.84 TADD743 pKa = 3.48 RR744 pKa = 11.84 RR745 pKa = 11.84 PRR747 pKa = 11.84 RR748 pKa = 11.84 ARR750 pKa = 11.84 AQRR753 pKa = 11.84 RR754 pKa = 11.84 RR755 pKa = 11.84 AGPGRR760 pKa = 11.84 SRR762 pKa = 11.84 RR763 pKa = 11.84 QRR765 pKa = 11.84 RR766 pKa = 11.84 PARR769 pKa = 11.84 NPTGTADD776 pKa = 3.99 RR777 pKa = 11.84 GPAGARR783 pKa = 11.84 RR784 pKa = 11.84 PAAPVRR790 pKa = 11.84 RR791 pKa = 11.84 RR792 pKa = 11.84 RR793 pKa = 11.84 RR794 pKa = 11.84 AARR797 pKa = 11.84 LRR799 pKa = 11.84 RR800 pKa = 11.84 RR801 pKa = 11.84 VHH803 pKa = 5.45 LRR805 pKa = 11.84 RR806 pKa = 11.84 RR807 pKa = 11.84 TAGRR811 pKa = 11.84 PRR813 pKa = 11.84 PRR815 pKa = 11.84 AGGGLAVPAGPAPLRR830 pKa = 11.84 GAAVAGAGGQHH841 pKa = 6.4 PVDD844 pKa = 3.75 GRR846 pKa = 11.84 AGEE849 pKa = 4.18 AVPVPGRR856 pKa = 11.84 RR857 pKa = 11.84 PGRR860 pKa = 11.84 ALGKK864 pKa = 10.01 RR865 pKa = 11.84 PAPAADD871 pKa = 3.27 VRR873 pKa = 11.84 DD874 pKa = 4.02 DD875 pKa = 3.4 TRR877 pKa = 11.84 VVHH880 pKa = 6.07 RR881 pKa = 11.84 HH882 pKa = 5.13 RR883 pKa = 11.84 LRR885 pKa = 11.84 PRR887 pKa = 11.84 PDD889 pKa = 2.89 RR890 pKa = 11.84 RR891 pKa = 11.84 VRR893 pKa = 11.84 APHH896 pKa = 5.62 RR897 pKa = 11.84 RR898 pKa = 11.84 QRR900 pKa = 11.84 RR901 pKa = 11.84 THH903 pKa = 6.19 RR904 pKa = 11.84 PARR907 pKa = 11.84 TAGRR911 pKa = 11.84 AGGDD915 pKa = 3.05 RR916 pKa = 11.84 RR917 pKa = 11.84 AGAGGPGTGRR927 pKa = 11.84 SRR929 pKa = 11.84 RR930 pKa = 11.84 RR931 pKa = 11.84 ADD933 pKa = 3.24 RR934 pKa = 11.84 RR935 pKa = 11.84 RR936 pKa = 11.84 RR937 pKa = 11.84 GADD940 pKa = 2.76 RR941 pKa = 11.84 GRR943 pKa = 11.84 ARR945 pKa = 11.84 TRR947 pKa = 11.84 RR948 pKa = 11.84 RR949 pKa = 11.84 TPHH952 pKa = 6.25 AGDD955 pKa = 3.14 RR956 pKa = 11.84 AQRR959 pKa = 11.84 VRR961 pKa = 11.84 DD962 pKa = 3.73 PGARR966 pKa = 11.84 QQQGRR971 pKa = 11.84 HH972 pKa = 4.82 RR973 pKa = 11.84 AAQTVAHH980 pKa = 6.41 PQVQVLQAPGILVPAQAVAFRR1001 pKa = 11.84 HH1002 pKa = 5.26 AA1003 pKa = 4.09
Molecular weight: 111.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.394
IPC2_protein 10.847
IPC_protein 12.471
Toseland 12.632
ProMoST 13.13
Dawson 12.632
Bjellqvist 12.632
Wikipedia 13.1
Rodwell 12.135
Grimsley 12.661
Solomon 13.13
Lehninger 13.027
Nozaki 12.632
DTASelect 12.632
Thurlkill 12.632
EMBOSS 13.13
Sillero 12.632
Patrickios 11.828
IPC_peptide 13.13
IPC2_peptide 12.12
IPC2.peptide.svr19 9.17
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8394
0
8394
2700982
49
5850
321.8
34.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.532 ± 0.043
0.78 ± 0.008
5.807 ± 0.021
5.668 ± 0.03
2.956 ± 0.017
9.243 ± 0.026
2.17 ± 0.012
3.298 ± 0.02
2.26 ± 0.019
10.442 ± 0.032
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.658 ± 0.012
1.928 ± 0.016
6.125 ± 0.024
2.879 ± 0.016
7.915 ± 0.034
5.359 ± 0.018
5.757 ± 0.021
8.705 ± 0.028
1.508 ± 0.011
2.008 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here