Amino acid dipepetide frequency for Aeromicrobium sp. Root495

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.738AlaAla: 17.738 ± 0.177
0.886AlaCys: 0.886 ± 0.03
8.364AlaAsp: 8.364 ± 0.093
7.795AlaGlu: 7.795 ± 0.117
3.679AlaPhe: 3.679 ± 0.068
11.353AlaGly: 11.353 ± 0.102
2.457AlaHis: 2.457 ± 0.058
4.264AlaIle: 4.264 ± 0.073
2.938AlaLys: 2.938 ± 0.066
13.266AlaLeu: 13.266 ± 0.12
2.653AlaMet: 2.653 ± 0.059
1.929AlaAsn: 1.929 ± 0.046
5.571AlaPro: 5.571 ± 0.1
3.841AlaGln: 3.841 ± 0.075
8.807AlaArg: 8.807 ± 0.095
7.037AlaSer: 7.037 ± 0.087
7.201AlaThr: 7.201 ± 0.097
11.785AlaVal: 11.785 ± 0.126
1.93AlaTrp: 1.93 ± 0.041
2.379AlaTyr: 2.379 ± 0.039
0.0AlaXaa: 0.0 ± 0.0
Cys
0.759CysAla: 0.759 ± 0.028
0.055CysCys: 0.055 ± 0.007
0.38CysAsp: 0.38 ± 0.017
0.303CysGlu: 0.303 ± 0.016
0.193CysPhe: 0.193 ± 0.014
0.712CysGly: 0.712 ± 0.023
0.15CysHis: 0.15 ± 0.012
0.205CysIle: 0.205 ± 0.014
0.122CysLys: 0.122 ± 0.01
0.589CysLeu: 0.589 ± 0.025
0.087CysMet: 0.087 ± 0.009
0.122CysAsn: 0.122 ± 0.012
0.361CysPro: 0.361 ± 0.021
0.141CysGln: 0.141 ± 0.012
0.462CysArg: 0.462 ± 0.021
0.423CysSer: 0.423 ± 0.02
0.427CysThr: 0.427 ± 0.02
0.51CysVal: 0.51 ± 0.021
0.078CysTrp: 0.078 ± 0.012
0.118CysTyr: 0.118 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
8.339AspAla: 8.339 ± 0.109
0.299AspCys: 0.299 ± 0.018
5.205AspAsp: 5.205 ± 0.087
4.534AspGlu: 4.534 ± 0.063
1.69AspPhe: 1.69 ± 0.046
6.457AspGly: 6.457 ± 0.086
1.623AspHis: 1.623 ± 0.04
1.92AspIle: 1.92 ± 0.041
1.277AspLys: 1.277 ± 0.04
7.303AspLeu: 7.303 ± 0.102
0.831AspMet: 0.831 ± 0.024
0.887AspAsn: 0.887 ± 0.03
4.263AspPro: 4.263 ± 0.066
1.83AspGln: 1.83 ± 0.043
4.789AspArg: 4.789 ± 0.063
2.635AspSer: 2.635 ± 0.053
2.942AspThr: 2.942 ± 0.049
7.122AspVal: 7.122 ± 0.099
0.929AspTrp: 0.929 ± 0.032
1.163AspTyr: 1.163 ± 0.034
0.0AspXaa: 0.0 ± 0.0
Glu
7.382GluAla: 7.382 ± 0.09
0.273GluCys: 0.273 ± 0.015
3.097GluAsp: 3.097 ± 0.055
3.109GluGlu: 3.109 ± 0.075
1.353GluPhe: 1.353 ± 0.042
4.397GluGly: 4.397 ± 0.07
1.687GluHis: 1.687 ± 0.041
2.535GluIle: 2.535 ± 0.046
1.718GluLys: 1.718 ± 0.043
6.42GluLeu: 6.42 ± 0.087
0.936GluMet: 0.936 ± 0.032
1.072GluAsn: 1.072 ± 0.036
3.244GluPro: 3.244 ± 0.074
2.441GluGln: 2.441 ± 0.056
5.236GluArg: 5.236 ± 0.083
3.097GluSer: 3.097 ± 0.057
2.993GluThr: 2.993 ± 0.054
5.307GluVal: 5.307 ± 0.073
0.764GluTrp: 0.764 ± 0.024
1.02GluTyr: 1.02 ± 0.034
0.0GluXaa: 0.0 ± 0.0
Phe
3.615PheAla: 3.615 ± 0.069
0.246PheCys: 0.246 ± 0.015
2.136PheAsp: 2.136 ± 0.047
1.68PheGlu: 1.68 ± 0.038
0.978PhePhe: 0.978 ± 0.034
3.033PheGly: 3.033 ± 0.06
0.551PheHis: 0.551 ± 0.022
0.911PheIle: 0.911 ± 0.029
0.572PheLys: 0.572 ± 0.022
2.573PheLeu: 2.573 ± 0.054
0.46PheMet: 0.46 ± 0.021
0.612PheAsn: 0.612 ± 0.025
1.261PhePro: 1.261 ± 0.034
0.675PheGln: 0.675 ± 0.021
1.733PheArg: 1.733 ± 0.039
1.777PheSer: 1.777 ± 0.037
2.0PheThr: 2.0 ± 0.045
2.794PheVal: 2.794 ± 0.051
0.428PheTrp: 0.428 ± 0.023
0.671PheTyr: 0.671 ± 0.029
0.0PheXaa: 0.0 ± 0.0
Gly
9.823GlyAla: 9.823 ± 0.09
0.686GlyCys: 0.686 ± 0.025
5.101GlyAsp: 5.101 ± 0.069
4.688GlyGlu: 4.688 ± 0.073
2.986GlyPhe: 2.986 ± 0.052
7.617GlyGly: 7.617 ± 0.104
2.046GlyHis: 2.046 ± 0.051
3.845GlyIle: 3.845 ± 0.054
2.363GlyLys: 2.363 ± 0.053
9.631GlyLeu: 9.631 ± 0.109
1.638GlyMet: 1.638 ± 0.041
1.565GlyAsn: 1.565 ± 0.04
4.346GlyPro: 4.346 ± 0.065
2.779GlyGln: 2.779 ± 0.055
6.867GlyArg: 6.867 ± 0.088
5.953GlySer: 5.953 ± 0.082
5.842GlyThr: 5.842 ± 0.089
7.896GlyVal: 7.896 ± 0.09
1.681GlyTrp: 1.681 ± 0.049
2.105GlyTyr: 2.105 ± 0.047
0.0GlyXaa: 0.0 ± 0.0
His
2.457HisAla: 2.457 ± 0.047
0.136HisCys: 0.136 ± 0.012
1.703HisAsp: 1.703 ± 0.042
1.363HisGlu: 1.363 ± 0.04
0.57HisPhe: 0.57 ± 0.023
2.232HisGly: 2.232 ± 0.045
0.688HisHis: 0.688 ± 0.026
0.51HisIle: 0.51 ± 0.023
0.301HisLys: 0.301 ± 0.016
2.365HisLeu: 2.365 ± 0.053
0.294HisMet: 0.294 ± 0.017
0.29HisAsn: 0.29 ± 0.018
1.483HisPro: 1.483 ± 0.04
0.627HisGln: 0.627 ± 0.024
1.723HisArg: 1.723 ± 0.039
0.911HisSer: 0.911 ± 0.025
1.057HisThr: 1.057 ± 0.03
2.272HisVal: 2.272 ± 0.046
0.288HisTrp: 0.288 ± 0.017
0.412HisTyr: 0.412 ± 0.021
0.0HisXaa: 0.0 ± 0.0
Ile
4.957IleAla: 4.957 ± 0.071
0.258IleCys: 0.258 ± 0.015
3.04IleAsp: 3.04 ± 0.052
2.482IleGlu: 2.482 ± 0.051
0.918IlePhe: 0.918 ± 0.036
3.758IleGly: 3.758 ± 0.067
0.654IleHis: 0.654 ± 0.025
1.299IleIle: 1.299 ± 0.037
0.913IleLys: 0.913 ± 0.035
2.708IleLeu: 2.708 ± 0.056
0.546IleMet: 0.546 ± 0.024
0.849IleAsn: 0.849 ± 0.03
1.679IlePro: 1.679 ± 0.044
0.853IleGln: 0.853 ± 0.027
2.107IleArg: 2.107 ± 0.044
2.049IleSer: 2.049 ± 0.04
2.45IleThr: 2.45 ± 0.047
3.446IleVal: 3.446 ± 0.063
0.384IleTrp: 0.384 ± 0.02
0.611IleTyr: 0.611 ± 0.024
0.0IleXaa: 0.0 ± 0.0
Lys
3.207LysAla: 3.207 ± 0.065
0.09LysCys: 0.09 ± 0.009
1.493LysAsp: 1.493 ± 0.041
1.108LysGlu: 1.108 ± 0.035
0.508LysPhe: 0.508 ± 0.02
1.94LysGly: 1.94 ± 0.047
0.492LysHis: 0.492 ± 0.023
1.113LysIle: 1.113 ± 0.035
1.084LysLys: 1.084 ± 0.05
1.984LysLeu: 1.984 ± 0.053
0.357LysMet: 0.357 ± 0.019
0.662LysAsn: 0.662 ± 0.028
1.411LysPro: 1.411 ± 0.043
0.777LysGln: 0.777 ± 0.03
1.487LysArg: 1.487 ± 0.043
1.301LysSer: 1.301 ± 0.041
1.497LysThr: 1.497 ± 0.038
2.345LysVal: 2.345 ± 0.066
0.216LysTrp: 0.216 ± 0.015
0.507LysTyr: 0.507 ± 0.028
0.0LysXaa: 0.0 ± 0.0
Leu
14.193LeuAla: 14.193 ± 0.139
0.588LeuCys: 0.588 ± 0.024
7.446LeuAsp: 7.446 ± 0.09
5.933LeuGlu: 5.933 ± 0.077
2.453LeuPhe: 2.453 ± 0.054
9.55LeuGly: 9.55 ± 0.108
2.101LeuHis: 2.101 ± 0.046
2.839LeuIle: 2.839 ± 0.06
2.114LeuLys: 2.114 ± 0.051
10.398LeuLeu: 10.398 ± 0.126
1.653LeuMet: 1.653 ± 0.042
1.652LeuAsn: 1.652 ± 0.044
5.457LeuPro: 5.457 ± 0.07
2.731LeuGln: 2.731 ± 0.062
7.567LeuArg: 7.567 ± 0.096
5.613LeuSer: 5.613 ± 0.075
6.28LeuThr: 6.28 ± 0.076
11.061LeuVal: 11.061 ± 0.129
1.256LeuTrp: 1.256 ± 0.037
1.509LeuTyr: 1.509 ± 0.036
0.0LeuXaa: 0.0 ± 0.0
Met
2.162MetAla: 2.162 ± 0.042
0.098MetCys: 0.098 ± 0.008
0.848MetAsp: 0.848 ± 0.028
0.722MetGlu: 0.722 ± 0.025
0.476MetPhe: 0.476 ± 0.018
1.309MetGly: 1.309 ± 0.039
0.365MetHis: 0.365 ± 0.016
0.693MetIle: 0.693 ± 0.022
0.533MetLys: 0.533 ± 0.022
1.776MetLeu: 1.776 ± 0.042
0.306MetMet: 0.306 ± 0.018
0.397MetAsn: 0.397 ± 0.02
0.998MetPro: 0.998 ± 0.028
0.467MetGln: 0.467 ± 0.02
1.321MetArg: 1.321 ± 0.038
1.58MetSer: 1.58 ± 0.037
1.719MetThr: 1.719 ± 0.041
1.385MetVal: 1.385 ± 0.038
0.17MetTrp: 0.17 ± 0.013
0.251MetTyr: 0.251 ± 0.016
0.0MetXaa: 0.0 ± 0.0
Asn
2.184AsnAla: 2.184 ± 0.045
0.103AsnCys: 0.103 ± 0.011
1.165AsnAsp: 1.165 ± 0.031
0.864AsnGlu: 0.864 ± 0.031
0.505AsnPhe: 0.505 ± 0.021
1.747AsnGly: 1.747 ± 0.042
0.365AsnHis: 0.365 ± 0.018
0.772AsnIle: 0.772 ± 0.031
0.42AsnLys: 0.42 ± 0.022
1.862AsnLeu: 1.862 ± 0.045
0.278AsnMet: 0.278 ± 0.017
0.441AsnAsn: 0.441 ± 0.021
1.298AsnPro: 1.298 ± 0.035
0.558AsnGln: 0.558 ± 0.026
1.235AsnArg: 1.235 ± 0.04
0.789AsnSer: 0.789 ± 0.028
0.995AsnThr: 0.995 ± 0.033
1.57AsnVal: 1.57 ± 0.039
0.213AsnTrp: 0.213 ± 0.015
0.369AsnTyr: 0.369 ± 0.019
0.0AsnXaa: 0.0 ± 0.0
Pro
6.376ProAla: 6.376 ± 0.092
0.255ProCys: 0.255 ± 0.014
4.198ProAsp: 4.198 ± 0.067
3.959ProGlu: 3.959 ± 0.076
1.656ProPhe: 1.656 ± 0.037
5.032ProGly: 5.032 ± 0.074
1.207ProHis: 1.207 ± 0.034
1.528ProIle: 1.528 ± 0.039
1.069ProLys: 1.069 ± 0.036
4.612ProLeu: 4.612 ± 0.072
0.916ProMet: 0.916 ± 0.027
0.816ProAsn: 0.816 ± 0.03
2.249ProPro: 2.249 ± 0.054
1.466ProGln: 1.466 ± 0.036
3.533ProArg: 3.533 ± 0.07
3.39ProSer: 3.39 ± 0.061
3.438ProThr: 3.438 ± 0.06
5.01ProVal: 5.01 ± 0.074
0.906ProTrp: 0.906 ± 0.031
1.069ProTyr: 1.069 ± 0.034
0.0ProXaa: 0.0 ± 0.0
Gln
3.819GlnAla: 3.819 ± 0.054
0.134GlnCys: 0.134 ± 0.011
1.601GlnAsp: 1.601 ± 0.04
1.48GlnGlu: 1.48 ± 0.044
0.685GlnPhe: 0.685 ± 0.022
2.296GlnGly: 2.296 ± 0.056
0.771GlnHis: 0.771 ± 0.03
1.11GlnIle: 1.11 ± 0.034
0.763GlnLys: 0.763 ± 0.028
3.306GlnLeu: 3.306 ± 0.056
0.551GlnMet: 0.551 ± 0.023
0.557GlnAsn: 0.557 ± 0.023
1.41GlnPro: 1.41 ± 0.035
1.157GlnGln: 1.157 ± 0.037
2.495GlnArg: 2.495 ± 0.053
1.482GlnSer: 1.482 ± 0.042
1.475GlnThr: 1.475 ± 0.04
3.037GlnVal: 3.037 ± 0.05
0.463GlnTrp: 0.463 ± 0.019
0.619GlnTyr: 0.619 ± 0.027
0.0GlnXaa: 0.0 ± 0.0
Arg
8.135ArgAla: 8.135 ± 0.098
0.397ArgCys: 0.397 ± 0.023
4.292ArgAsp: 4.292 ± 0.067
4.358ArgGlu: 4.358 ± 0.078
2.264ArgPhe: 2.264 ± 0.047
5.418ArgGly: 5.418 ± 0.076
1.761ArgHis: 1.761 ± 0.038
3.027ArgIle: 3.027 ± 0.057
1.556ArgLys: 1.556 ± 0.037
8.148ArgLeu: 8.148 ± 0.096
1.608ArgMet: 1.608 ± 0.041
1.274ArgAsn: 1.274 ± 0.038
4.086ArgPro: 4.086 ± 0.075
2.175ArgGln: 2.175 ± 0.05
6.812ArgArg: 6.812 ± 0.088
4.681ArgSer: 4.681 ± 0.063
4.714ArgThr: 4.714 ± 0.064
6.075ArgVal: 6.075 ± 0.084
1.336ArgTrp: 1.336 ± 0.039
1.533ArgTyr: 1.533 ± 0.042
0.0ArgXaa: 0.0 ± 0.0
Ser
7.115SerAla: 7.115 ± 0.098
0.412SerCys: 0.412 ± 0.019
3.581SerAsp: 3.581 ± 0.062
2.851SerGlu: 2.851 ± 0.059
1.924SerPhe: 1.924 ± 0.047
6.0SerGly: 6.0 ± 0.079
1.017SerHis: 1.017 ± 0.031
2.25SerIle: 2.25 ± 0.049
1.311SerLys: 1.311 ± 0.041
5.541SerLeu: 5.541 ± 0.079
1.281SerMet: 1.281 ± 0.035
1.068SerAsn: 1.068 ± 0.035
3.086SerPro: 3.086 ± 0.057
1.541SerGln: 1.541 ± 0.035
3.971SerArg: 3.971 ± 0.063
4.017SerSer: 4.017 ± 0.089
4.052SerThr: 4.052 ± 0.076
5.082SerVal: 5.082 ± 0.069
0.985SerTrp: 0.985 ± 0.029
1.237SerTyr: 1.237 ± 0.032
0.0SerXaa: 0.0 ± 0.0
Thr
7.28ThrAla: 7.28 ± 0.088
0.393ThrCys: 0.393 ± 0.022
3.697ThrAsp: 3.697 ± 0.067
3.067ThrGlu: 3.067 ± 0.054
2.186ThrPhe: 2.186 ± 0.046
5.616ThrGly: 5.616 ± 0.077
1.168ThrHis: 1.168 ± 0.03
2.614ThrIle: 2.614 ± 0.053
1.403ThrLys: 1.403 ± 0.043
5.838ThrLeu: 5.838 ± 0.084
1.047ThrMet: 1.047 ± 0.028
1.09ThrAsn: 1.09 ± 0.027
3.653ThrPro: 3.653 ± 0.064
1.65ThrGln: 1.65 ± 0.04
3.839ThrArg: 3.839 ± 0.057
4.092ThrSer: 4.092 ± 0.061
4.217ThrThr: 4.217 ± 0.082
6.08ThrVal: 6.08 ± 0.091
1.065ThrTrp: 1.065 ± 0.034
1.51ThrTyr: 1.51 ± 0.043
0.0ThrXaa: 0.0 ± 0.0
Val
12.455ValAla: 12.455 ± 0.119
0.598ValCys: 0.598 ± 0.021
6.348ValAsp: 6.348 ± 0.088
6.091ValGlu: 6.091 ± 0.088
2.454ValPhe: 2.454 ± 0.053
8.064ValGly: 8.064 ± 0.099
1.943ValHis: 1.943 ± 0.045
3.404ValIle: 3.404 ± 0.064
2.304ValLys: 2.304 ± 0.052
10.35ValLeu: 10.35 ± 0.108
1.622ValMet: 1.622 ± 0.045
1.738ValAsn: 1.738 ± 0.04
5.179ValPro: 5.179 ± 0.075
2.479ValGln: 2.479 ± 0.048
6.86ValArg: 6.86 ± 0.083
5.327ValSer: 5.327 ± 0.071
6.096ValThr: 6.096 ± 0.083
11.287ValVal: 11.287 ± 0.135
1.131ValTrp: 1.131 ± 0.033
1.463ValTyr: 1.463 ± 0.036
0.0ValXaa: 0.0 ± 0.0
Trp
1.519TrpAla: 1.519 ± 0.039
0.141TrpCys: 0.141 ± 0.015
0.896TrpAsp: 0.896 ± 0.03
0.582TrpGlu: 0.582 ± 0.026
0.575TrpPhe: 0.575 ± 0.027
1.078TrpGly: 1.078 ± 0.035
0.329TrpHis: 0.329 ± 0.019
0.64TrpIle: 0.64 ± 0.026
0.394TrpLys: 0.394 ± 0.018
1.659TrpLeu: 1.659 ± 0.046
0.292TrpMet: 0.292 ± 0.017
0.34TrpAsn: 0.34 ± 0.018
0.726TrpPro: 0.726 ± 0.027
0.501TrpGln: 0.501 ± 0.021
1.244TrpArg: 1.244 ± 0.038
1.097TrpSer: 1.097 ± 0.034
1.038TrpThr: 1.038 ± 0.033
1.206TrpVal: 1.206 ± 0.035
0.369TrpTrp: 0.369 ± 0.019
0.282TrpTyr: 0.282 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.356TyrAla: 2.356 ± 0.046
0.15TyrCys: 0.15 ± 0.011
1.448TyrAsp: 1.448 ± 0.035
1.113TyrGlu: 1.113 ± 0.036
0.656TyrPhe: 0.656 ± 0.025
1.849TyrGly: 1.849 ± 0.044
0.307TyrHis: 0.307 ± 0.018
0.482TyrIle: 0.482 ± 0.022
0.424TyrLys: 0.424 ± 0.022
2.063TyrLeu: 2.063 ± 0.049
0.221TyrMet: 0.221 ± 0.014
0.37TyrAsn: 0.37 ± 0.021
0.922TyrPro: 0.922 ± 0.032
0.52TyrGln: 0.52 ± 0.025
1.562TyrArg: 1.562 ± 0.039
1.074TyrSer: 1.074 ± 0.034
1.07TyrThr: 1.07 ± 0.034
1.923TyrVal: 1.923 ± 0.039
0.317TyrTrp: 0.317 ± 0.017
0.427TyrTyr: 0.427 ± 0.019
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3422 proteins (1067815 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski