Sulfolobales Beppu rod-shaped virus 1
Average proteome isoelectric point is 7.05
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3S8NF50|A0A3S8NF50_9VIRU Ribbon-helix-helix domain OS=Sulfolobales Beppu rod-shaped virus 1 OX=2493121 GN=SBRV1_gp08 PE=4 SV=1
MM1 pKa = 6.65 WQDD4 pKa = 3.38 IIEE7 pKa = 4.14 KK8 pKa = 9.07 MKK10 pKa = 10.69 RR11 pKa = 11.84 CIDD14 pKa = 4.03 EE15 pKa = 4.39 NCLLQVLHH23 pKa = 6.78 EE24 pKa = 4.79 CYY26 pKa = 10.32 EE27 pKa = 3.9 NCKK30 pKa = 10.56 DD31 pKa = 4.53 DD32 pKa = 3.92 EE33 pKa = 4.76 CEE35 pKa = 4.44 SICNMLYY42 pKa = 10.17 EE43 pKa = 4.35 EE44 pKa = 4.74 RR45 pKa = 11.84 SRR47 pKa = 11.84 DD48 pKa = 3.69 LEE50 pKa = 4.19 FF51 pKa = 5.88
Molecular weight: 6.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.074
IPC2_protein 4.368
IPC_protein 4.228
Toseland 4.062
ProMoST 4.342
Dawson 4.164
Bjellqvist 4.329
Wikipedia 4.05
Rodwell 4.062
Grimsley 3.986
Solomon 4.164
Lehninger 4.113
Nozaki 4.291
DTASelect 4.406
Thurlkill 4.088
EMBOSS 4.062
Sillero 4.329
Patrickios 0.896
IPC_peptide 4.164
IPC2_peptide 4.317
IPC2.peptide.svr19 4.245
Protein with the highest isoelectric point:
>tr|A0A3Q8Q418|A0A3Q8Q418_9VIRU Ribbon-helix-helix domain OS=Sulfolobales Beppu rod-shaped virus 1 OX=2493121 GN=SBRV1_gp41 PE=4 SV=1
MM1 pKa = 7.69 AKK3 pKa = 10.1 GHH5 pKa = 5.63 TPRR8 pKa = 11.84 SFAQRR13 pKa = 11.84 YY14 pKa = 7.67 AKK16 pKa = 10.06 WSAKK20 pKa = 9.73 FNAFSNPTVASTILGNVAPTAQQNFQTHH48 pKa = 4.36 VTKK51 pKa = 9.8 FTSVNEE57 pKa = 3.98 QVNAVLTQYY66 pKa = 11.32 GVTGPNRR73 pKa = 11.84 AIYY76 pKa = 9.39 QGYY79 pKa = 8.38 GMKK82 pKa = 10.05 VARR85 pKa = 11.84 ALNRR89 pKa = 11.84 LGQGSALANMIAGLKK104 pKa = 9.89 AYY106 pKa = 9.66 YY107 pKa = 10.07 INAFNANPQILDD119 pKa = 3.32 AVTNIITGSPTGYY132 pKa = 10.13 ISS134 pKa = 3.26
Molecular weight: 14.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.24
IPC2_protein 10.16
IPC_protein 10.467
Toseland 10.233
ProMoST 10.028
Dawson 10.482
Bjellqvist 10.189
Wikipedia 10.687
Rodwell 10.847
Grimsley 10.57
Solomon 10.511
Lehninger 10.467
Nozaki 10.204
DTASelect 10.189
Thurlkill 10.306
EMBOSS 10.643
Sillero 10.379
Patrickios 10.496
IPC_peptide 10.496
IPC2_peptide 8.99
IPC2.peptide.svr19 8.462
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
56
0
56
9831
34
1064
175.6
20.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.109 ± 0.338
0.926 ± 0.171
4.547 ± 0.35
6.042 ± 0.52
6.093 ± 0.275
3.743 ± 0.233
1.424 ± 0.18
10.263 ± 0.423
8.229 ± 0.748
10.528 ± 0.546
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.116 ± 0.219
7.242 ± 0.517
3.052 ± 0.289
5.218 ± 0.455
2.899 ± 0.257
6.174 ± 0.447
4.76 ± 0.261
5.513 ± 0.257
0.844 ± 0.108
6.276 ± 0.259
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here