Bacillus phage DK2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Salasmaviridae; Northropvirinae; Hemphillvirus; Bacillus virus DK2

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 45 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3T0IJ45|A0A3T0IJ45_9CAUD Tail protein OS=Bacillus phage DK2 OX=2500809 GN=DK2_000031 PE=4 SV=1
MM1 pKa = 7.4EE2 pKa = 5.56NEE4 pKa = 3.49LDD6 pKa = 4.74FYY8 pKa = 10.98IDD10 pKa = 3.61EE11 pKa = 4.72NGSKK15 pKa = 10.53VFILKK20 pKa = 10.3EE21 pKa = 3.86YY22 pKa = 10.6DD23 pKa = 3.6PQDD26 pKa = 3.84DD27 pKa = 4.13AEE29 pKa = 4.11QQ30 pKa = 3.7

Molecular weight: 3.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3T0IIZ2|A0A3T0IIZ2_9CAUD Terminal protein OS=Bacillus phage DK2 OX=2500809 GN=DK2_000018 PE=4 SV=1
MM1 pKa = 7.67NINKK5 pKa = 8.24VFPLEE10 pKa = 3.9NKK12 pKa = 9.32FRR14 pKa = 11.84LRR16 pKa = 11.84KK17 pKa = 10.1GKK19 pKa = 8.59MLYY22 pKa = 9.98PRR24 pKa = 11.84TKK26 pKa = 10.2PLFKK30 pKa = 10.22RR31 pKa = 11.84YY32 pKa = 9.19LQIHH36 pKa = 4.98YY37 pKa = 10.06LIRR40 pKa = 11.84KK41 pKa = 8.41NSNPSNN47 pKa = 3.6

Molecular weight: 5.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

45

0

45

7453

30

778

165.6

19.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.213 ± 0.417

1.127 ± 0.154

6.829 ± 0.304

7.943 ± 0.462

4.468 ± 0.267

6.333 ± 0.393

1.744 ± 0.154

7.017 ± 0.398

9.285 ± 0.533

7.178 ± 0.301

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.421 ± 0.257

6.829 ± 0.401

2.603 ± 0.285

3.475 ± 0.223

4.32 ± 0.299

4.884 ± 0.204

5.796 ± 0.483

6.467 ± 0.366

1.315 ± 0.192

4.75 ± 0.216
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski