Arteriviridae sp.
Average proteome isoelectric point is 7.65
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6B9PVZ4|A0A6B9PVZ4_9NIDO Envelope glycoprotein 4 OS=Arteriviridae sp. OX=2697055 PE=4 SV=1
MM1 pKa = 7.61 GSALLLLMASAVGIVGVAAIACRR24 pKa = 11.84 PCYY27 pKa = 9.73 GSHH30 pKa = 7.14 YY31 pKa = 10.29 IDD33 pKa = 3.5 TAKK36 pKa = 10.49 KK37 pKa = 10.14 DD38 pKa = 3.84 SQLTAPVPVHH48 pKa = 6.51 LFCKK52 pKa = 10.23 QDD54 pKa = 3.87 DD55 pKa = 4.06 EE56 pKa = 4.59 AKK58 pKa = 10.52 KK59 pKa = 10.21 IQSDD63 pKa = 3.71 QAKK66 pKa = 8.54 VGIRR70 pKa = 11.84 GVVCPVGDD78 pKa = 3.47 QLAFKK83 pKa = 9.93 IQDD86 pKa = 3.53 VVEE89 pKa = 4.71 LKK91 pKa = 10.86 DD92 pKa = 3.76 EE93 pKa = 4.43 SSSASQDD100 pKa = 3.02 ALFFTACLIHH110 pKa = 7.22 ALDD113 pKa = 3.71 MAEE116 pKa = 4.83 HH117 pKa = 6.8 GFHH120 pKa = 7.45 PYY122 pKa = 9.45 FANCSGIPCTCVSITEE138 pKa = 4.39 WVAHH142 pKa = 5.39 VKK144 pKa = 10.2 NATGAPDD151 pKa = 3.42 HH152 pKa = 6.47 EE153 pKa = 5.17 RR154 pKa = 11.84 GLFTKK159 pKa = 7.51 FTPHH163 pKa = 6.11 GLRR166 pKa = 11.84 WATVVTCVIAVLAAWLPP183 pKa = 3.8
Molecular weight: 19.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.745
IPC2_protein 5.817
IPC_protein 5.881
Toseland 6.211
ProMoST 6.313
Dawson 6.224
Bjellqvist 6.211
Wikipedia 6.249
Rodwell 6.224
Grimsley 6.326
Solomon 6.237
Lehninger 6.237
Nozaki 6.517
DTASelect 6.693
Thurlkill 6.722
EMBOSS 6.693
Sillero 6.62
Patrickios 3.503
IPC_peptide 6.249
IPC2_peptide 6.605
IPC2.peptide.svr19 6.519
Protein with the highest isoelectric point:
>tr|A0A6B9PJ64|A0A6B9PJ64_9NIDO Envelope protein E OS=Arteriviridae sp. OX=2697055 PE=4 SV=1
MM1 pKa = 7.35 GASLDD6 pKa = 4.26 DD7 pKa = 3.71 FCLDD11 pKa = 3.18 HH12 pKa = 6.74 TAVQKK17 pKa = 11.0 VILAFSITYY26 pKa = 7.83 TPVMIYY32 pKa = 10.38 ALKK35 pKa = 9.92 VSRR38 pKa = 11.84 GRR40 pKa = 11.84 LLGILHH46 pKa = 7.17 LLIFLNCAFTFGYY59 pKa = 7.32 MTFVHH64 pKa = 7.03 FKK66 pKa = 9.01 STNTVALSMGAIVAVLWGTYY86 pKa = 8.1 SAIEE90 pKa = 3.7 TWRR93 pKa = 11.84 FVVARR98 pKa = 11.84 CRR100 pKa = 11.84 LCFLGRR106 pKa = 11.84 KK107 pKa = 9.14 YY108 pKa = 10.48 ILAPAHH114 pKa = 6.13 HH115 pKa = 6.87 VEE117 pKa = 4.55 SSAGLHH123 pKa = 6.72 PIPAKK128 pKa = 10.45 DD129 pKa = 3.12 NHH131 pKa = 6.3 AFVVRR136 pKa = 11.84 KK137 pKa = 9.44 PGLTSVNGTLVPGLRR152 pKa = 11.84 NLVLGGRR159 pKa = 11.84 RR160 pKa = 11.84 AVKK163 pKa = 9.96 QGVVNLVKK171 pKa = 10.55 YY172 pKa = 10.82 AKK174 pKa = 10.4
Molecular weight: 19.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.284
IPC2_protein 9.575
IPC_protein 9.794
Toseland 10.189
ProMoST 9.94
Dawson 10.379
Bjellqvist 10.087
Wikipedia 10.555
Rodwell 10.774
Grimsley 10.452
Solomon 10.409
Lehninger 10.379
Nozaki 10.233
DTASelect 10.058
Thurlkill 10.233
EMBOSS 10.584
Sillero 10.306
Patrickios 10.452
IPC_peptide 10.409
IPC2_peptide 9.092
IPC2.peptide.svr19 8.471
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10
0
10
5213
45
2491
521.3
57.43
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.21 ± 0.411
3.53 ± 0.32
4.777 ± 0.566
3.971 ± 0.64
4.259 ± 0.508
7.021 ± 0.487
2.916 ± 0.38
4.297 ± 0.542
4.585 ± 0.374
9.764 ± 0.812
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.091 ± 0.134
2.762 ± 0.238
6.791 ± 1.086
3.721 ± 0.555
5.064 ± 0.384
6.445 ± 0.44
6.676 ± 0.238
8.421 ± 0.384
1.822 ± 0.274
2.877 ± 0.48
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here