Amino acid dipepetide frequency for Nocardioides sp. CF8

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	18.357AlaAla: 18.357 ± 0.179	1.003AlaCys: 1.003 ± 0.029	8.631AlaAsp: 8.631 ± 0.099	7.889AlaGlu: 7.889 ± 0.09	3.628AlaPhe: 3.628 ± 0.055	11.812AlaGly: 11.812 ± 0.1	2.648AlaHis: 2.648 ± 0.049	4.905AlaIle: 4.905 ± 0.069	2.862AlaLys: 2.862 ± 0.066	13.227AlaLeu: 13.227 ± 0.143	2.967AlaMet: 2.967 ± 0.047	2.236AlaAsn: 2.236 ± 0.044	5.907AlaPro: 5.907 ± 0.075	3.615AlaGln: 3.615 ± 0.057	8.914AlaArg: 8.914 ± 0.096	6.54AlaSer: 6.54 ± 0.079	7.749AlaThr: 7.749 ± 0.083	10.857AlaVal: 10.857 ± 0.115	1.888AlaTrp: 1.888 ± 0.042	2.388AlaTyr: 2.388 ± 0.041	0.0AlaXaa: 0.0 ± 0.0
Cys	0.915CysAla: 0.915 ± 0.029	0.077CysCys: 0.077 ± 0.009	0.483CysAsp: 0.483 ± 0.02	0.424CysGlu: 0.424 ± 0.019	0.227CysPhe: 0.227 ± 0.013	0.86CysGly: 0.86 ± 0.027	0.202CysHis: 0.202 ± 0.012	0.238CysIle: 0.238 ± 0.015	0.129CysLys: 0.129 ± 0.009	0.681CysLeu: 0.681 ± 0.022	0.124CysMet: 0.124 ± 0.01	0.145CysAsn: 0.145 ± 0.011	0.435CysPro: 0.435 ± 0.018	0.182CysGln: 0.182 ± 0.012	0.551CysArg: 0.551 ± 0.02	0.465CysSer: 0.465 ± 0.019	0.482CysThr: 0.482 ± 0.022	0.596CysVal: 0.596 ± 0.025	0.112CysTrp: 0.112 ± 0.011	0.135CysTyr: 0.135 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	7.897AspAla: 7.897 ± 0.093	0.417AspCys: 0.417 ± 0.02	4.527AspAsp: 4.527 ± 0.073	4.636AspGlu: 4.636 ± 0.067	1.845AspPhe: 1.845 ± 0.042	6.216AspGly: 6.216 ± 0.077	1.575AspHis: 1.575 ± 0.038	2.357AspIle: 2.357 ± 0.044	1.366AspLys: 1.366 ± 0.035	7.407AspLeu: 7.407 ± 0.077	0.98AspMet: 0.98 ± 0.029	1.243AspAsn: 1.243 ± 0.029	4.375AspPro: 4.375 ± 0.065	1.948AspGln: 1.948 ± 0.037	4.725AspArg: 4.725 ± 0.06	2.774AspSer: 2.774 ± 0.048	3.13AspThr: 3.13 ± 0.052	6.131AspVal: 6.131 ± 0.07	1.026AspTrp: 1.026 ± 0.032	1.314AspTyr: 1.314 ± 0.037	0.0AspXaa: 0.0 ± 0.0
Glu	7.226GluAla: 7.226 ± 0.081	0.349GluCys: 0.349 ± 0.015	3.215GluAsp: 3.215 ± 0.051	3.29GluGlu: 3.29 ± 0.063	1.643GluPhe: 1.643 ± 0.036	4.42GluGly: 4.42 ± 0.064	1.777GluHis: 1.777 ± 0.036	2.768GluIle: 2.768 ± 0.047	1.476GluLys: 1.476 ± 0.041	6.541GluLeu: 6.541 ± 0.076	1.072GluMet: 1.072 ± 0.029	0.917GluAsn: 0.917 ± 0.024	3.137GluPro: 3.137 ± 0.061	2.317GluGln: 2.317 ± 0.047	5.042GluArg: 5.042 ± 0.072	3.003GluSer: 3.003 ± 0.049	3.129GluThr: 3.129 ± 0.051	5.365GluVal: 5.365 ± 0.078	0.881GluTrp: 0.881 ± 0.031	0.87GluTyr: 0.87 ± 0.029	0.0GluXaa: 0.0 ± 0.0
Phe	3.797PheAla: 3.797 ± 0.055	0.275PheCys: 0.275 ± 0.016	2.248PheAsp: 2.248 ± 0.043	1.783PheGlu: 1.783 ± 0.039	0.981PhePhe: 0.981 ± 0.033	3.11PheGly: 3.11 ± 0.046	0.654PheHis: 0.654 ± 0.025	0.981PheIle: 0.981 ± 0.032	0.598PheLys: 0.598 ± 0.021	2.657PheLeu: 2.657 ± 0.049	0.502PheMet: 0.502 ± 0.022	0.672PheAsn: 0.672 ± 0.024	1.323PhePro: 1.323 ± 0.034	0.65PheGln: 0.65 ± 0.025	1.691PheArg: 1.691 ± 0.04	1.531PheSer: 1.531 ± 0.037	1.937PheThr: 1.937 ± 0.036	2.698PheVal: 2.698 ± 0.046	0.435PheTrp: 0.435 ± 0.021	0.614PheTyr: 0.614 ± 0.023	0.0PheXaa: 0.0 ± 0.0
Gly	10.107GlyAla: 10.107 ± 0.106	0.803GlyCys: 0.803 ± 0.026	5.656GlyAsp: 5.656 ± 0.075	5.146GlyGlu: 5.146 ± 0.072	3.041GlyPhe: 3.041 ± 0.044	7.597GlyGly: 7.597 ± 0.095	2.208GlyHis: 2.208 ± 0.053	3.833GlyIle: 3.833 ± 0.055	2.325GlyLys: 2.325 ± 0.051	9.322GlyLeu: 9.322 ± 0.105	1.953GlyMet: 1.953 ± 0.037	1.766GlyAsn: 1.766 ± 0.041	4.129GlyPro: 4.129 ± 0.064	2.725GlyGln: 2.725 ± 0.049	6.6GlyArg: 6.6 ± 0.088	5.411GlySer: 5.411 ± 0.072	5.513GlyThr: 5.513 ± 0.077	7.859GlyVal: 7.859 ± 0.078	1.661GlyTrp: 1.661 ± 0.044	2.072GlyTyr: 2.072 ± 0.042	0.0GlyXaa: 0.0 ± 0.0
His	2.652HisAla: 2.652 ± 0.051	0.19HisCys: 0.19 ± 0.013	1.683HisAsp: 1.683 ± 0.04	1.334HisGlu: 1.334 ± 0.035	0.64HisPhe: 0.64 ± 0.022	2.234HisGly: 2.234 ± 0.046	0.764HisHis: 0.764 ± 0.026	0.667HisIle: 0.667 ± 0.024	0.405HisLys: 0.405 ± 0.018	2.479HisLeu: 2.479 ± 0.049	0.358HisMet: 0.358 ± 0.019	0.417HisAsn: 0.417 ± 0.022	1.522HisPro: 1.522 ± 0.033	0.64HisGln: 0.64 ± 0.024	1.816HisArg: 1.816 ± 0.039	1.001HisSer: 1.001 ± 0.027	1.25HisThr: 1.25 ± 0.032	2.056HisVal: 2.056 ± 0.038	0.331HisTrp: 0.331 ± 0.015	0.479HisTyr: 0.479 ± 0.017	0.0HisXaa: 0.0 ± 0.0
Ile	5.606IleAla: 5.606 ± 0.076	0.335IleCys: 0.335 ± 0.017	3.15IleAsp: 3.15 ± 0.052	2.754IleGlu: 2.754 ± 0.05	0.956IlePhe: 0.956 ± 0.03	4.1IleGly: 4.1 ± 0.06	0.793IleHis: 0.793 ± 0.025	1.241IleIle: 1.241 ± 0.039	0.94IleLys: 0.94 ± 0.032	2.798IleLeu: 2.798 ± 0.048	0.556IleMet: 0.556 ± 0.021	0.985IleAsn: 0.985 ± 0.029	1.887IlePro: 1.887 ± 0.039	0.901IleGln: 0.901 ± 0.028	2.369IleArg: 2.369 ± 0.04	2.171IleSer: 2.171 ± 0.046	2.619IleThr: 2.619 ± 0.045	3.47IleVal: 3.47 ± 0.049	0.445IleTrp: 0.445 ± 0.017	0.662IleTyr: 0.662 ± 0.026	0.0IleXaa: 0.0 ± 0.0
Lys	2.883LysAla: 2.883 ± 0.06	0.119LysCys: 0.119 ± 0.01	1.319LysAsp: 1.319 ± 0.038	1.094LysGlu: 1.094 ± 0.034	0.601LysPhe: 0.601 ± 0.024	1.729LysGly: 1.729 ± 0.044	0.487LysHis: 0.487 ± 0.019	1.006LysIle: 1.006 ± 0.028	0.872LysLys: 0.872 ± 0.037	2.023LysLeu: 2.023 ± 0.045	0.456LysMet: 0.456 ± 0.023	0.487LysAsn: 0.487 ± 0.026	1.303LysPro: 1.303 ± 0.035	0.778LysGln: 0.778 ± 0.026	1.558LysArg: 1.558 ± 0.041	1.25LysSer: 1.25 ± 0.039	1.37LysThr: 1.37 ± 0.036	2.226LysVal: 2.226 ± 0.042	0.251LysTrp: 0.251 ± 0.015	0.445LysTyr: 0.445 ± 0.019	0.0LysXaa: 0.0 ± 0.0
Leu	14.957LeuAla: 14.957 ± 0.151	0.66LeuCys: 0.66 ± 0.022	7.224LeuAsp: 7.224 ± 0.078	5.51LeuGlu: 5.51 ± 0.068	2.505LeuPhe: 2.505 ± 0.048	9.553LeuGly: 9.553 ± 0.096	2.088LeuHis: 2.088 ± 0.041	3.517LeuIle: 3.517 ± 0.057	1.984LeuLys: 1.984 ± 0.049	10.26LeuLeu: 10.26 ± 0.129	1.814LeuMet: 1.814 ± 0.041	1.776LeuAsn: 1.776 ± 0.041	5.658LeuPro: 5.658 ± 0.071	2.299LeuGln: 2.299 ± 0.046	7.376LeuArg: 7.376 ± 0.082	5.474LeuSer: 5.474 ± 0.073	6.506LeuThr: 6.506 ± 0.076	10.123LeuVal: 10.123 ± 0.099	1.088LeuTrp: 1.088 ± 0.029	1.48LeuTyr: 1.48 ± 0.035	0.0LeuXaa: 0.0 ± 0.0
Met	2.377MetAla: 2.377 ± 0.039	0.164MetCys: 0.164 ± 0.011	0.955MetAsp: 0.955 ± 0.028	0.86MetGlu: 0.86 ± 0.026	0.583MetPhe: 0.583 ± 0.019	1.517MetGly: 1.517 ± 0.039	0.419MetHis: 0.419 ± 0.019	0.759MetIle: 0.759 ± 0.027	0.55MetLys: 0.55 ± 0.022	2.072MetLeu: 2.072 ± 0.037	0.394MetMet: 0.394 ± 0.017	0.444MetAsn: 0.444 ± 0.019	1.078MetPro: 1.078 ± 0.031	0.543MetGln: 0.543 ± 0.023	1.53MetArg: 1.53 ± 0.031	1.514MetSer: 1.514 ± 0.033	1.823MetThr: 1.823 ± 0.036	1.608MetVal: 1.608 ± 0.036	0.237MetTrp: 0.237 ± 0.013	0.278MetTyr: 0.278 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	2.296AsnAla: 2.296 ± 0.047	0.162AsnCys: 0.162 ± 0.012	1.248AsnAsp: 1.248 ± 0.041	0.995AsnGlu: 0.995 ± 0.029	0.536AsnPhe: 0.536 ± 0.02	1.836AsnGly: 1.836 ± 0.046	0.451AsnHis: 0.451 ± 0.019	0.792AsnIle: 0.792 ± 0.025	0.475AsnLys: 0.475 ± 0.022	1.904AsnLeu: 1.904 ± 0.042	0.336AsnMet: 0.336 ± 0.015	0.482AsnAsn: 0.482 ± 0.023	1.484AsnPro: 1.484 ± 0.035	0.574AsnGln: 0.574 ± 0.022	1.278AsnArg: 1.278 ± 0.03	0.873AsnSer: 0.873 ± 0.029	1.109AsnThr: 1.109 ± 0.035	1.52AsnVal: 1.52 ± 0.039	0.292AsnTrp: 0.292 ± 0.015	0.425AsnTyr: 0.425 ± 0.02	0.0AsnXaa: 0.0 ± 0.0
Pro	6.867ProAla: 6.867 ± 0.093	0.287ProCys: 0.287 ± 0.016	4.228ProAsp: 4.228 ± 0.063	3.74ProGlu: 3.74 ± 0.056	1.491ProPhe: 1.491 ± 0.038	5.335ProGly: 5.335 ± 0.068	1.234ProHis: 1.234 ± 0.035	1.843ProIle: 1.843 ± 0.037	1.094ProLys: 1.094 ± 0.036	4.651ProLeu: 4.651 ± 0.066	1.097ProMet: 1.097 ± 0.03	0.906ProAsn: 0.906 ± 0.027	2.581ProPro: 2.581 ± 0.049	1.37ProGln: 1.37 ± 0.039	3.424ProArg: 3.424 ± 0.053	3.119ProSer: 3.119 ± 0.053	3.757ProThr: 3.757 ± 0.07	4.848ProVal: 4.848 ± 0.066	0.886ProTrp: 0.886 ± 0.027	1.119ProTyr: 1.119 ± 0.035	0.0ProXaa: 0.0 ± 0.0
Gln	3.677GlnAla: 3.677 ± 0.056	0.187GlnCys: 0.187 ± 0.013	1.301GlnAsp: 1.301 ± 0.033	1.397GlnGlu: 1.397 ± 0.037	0.766GlnPhe: 0.766 ± 0.024	2.163GlnGly: 2.163 ± 0.046	0.72GlnHis: 0.72 ± 0.025	1.224GlnIle: 1.224 ± 0.036	0.575GlnLys: 0.575 ± 0.022	3.088GlnLeu: 3.088 ± 0.055	0.592GlnMet: 0.592 ± 0.018	0.455GlnAsn: 0.455 ± 0.018	1.666GlnPro: 1.666 ± 0.036	1.176GlnGln: 1.176 ± 0.032	2.481GlnArg: 2.481 ± 0.05	1.408GlnSer: 1.408 ± 0.033	1.475GlnThr: 1.475 ± 0.042	2.854GlnVal: 2.854 ± 0.048	0.469GlnTrp: 0.469 ± 0.019	0.436GlnTyr: 0.436 ± 0.02	0.0GlnXaa: 0.0 ± 0.0
Arg	8.37ArgAla: 8.37 ± 0.096	0.491ArgCys: 0.491 ± 0.021	4.555ArgAsp: 4.555 ± 0.062	4.351ArgGlu: 4.351 ± 0.066	2.278ArgPhe: 2.278 ± 0.045	5.396ArgGly: 5.396 ± 0.069	1.825ArgHis: 1.825 ± 0.045	3.192ArgIle: 3.192 ± 0.054	1.601ArgLys: 1.601 ± 0.042	7.883ArgLeu: 7.883 ± 0.09	1.711ArgMet: 1.711 ± 0.037	1.402ArgAsn: 1.402 ± 0.036	3.912ArgPro: 3.912 ± 0.057	2.167ArgGln: 2.167 ± 0.041	6.745ArgArg: 6.745 ± 0.083	4.177ArgSer: 4.177 ± 0.062	4.403ArgThr: 4.403 ± 0.055	5.814ArgVal: 5.814 ± 0.078	1.288ArgTrp: 1.288 ± 0.034	1.462ArgTyr: 1.462 ± 0.034	0.0ArgXaa: 0.0 ± 0.0
Ser	6.548SerAla: 6.548 ± 0.081	0.431SerCys: 0.431 ± 0.018	3.239SerAsp: 3.239 ± 0.05	2.77SerGlu: 2.77 ± 0.047	1.787SerPhe: 1.787 ± 0.041	5.522SerGly: 5.522 ± 0.069	1.127SerHis: 1.127 ± 0.03	2.061SerIle: 2.061 ± 0.042	1.097SerLys: 1.097 ± 0.034	5.487SerLeu: 5.487 ± 0.066	1.43SerMet: 1.43 ± 0.029	1.031SerAsn: 1.031 ± 0.026	3.091SerPro: 3.091 ± 0.047	1.496SerGln: 1.496 ± 0.039	3.795SerArg: 3.795 ± 0.064	3.336SerSer: 3.336 ± 0.063	3.748SerThr: 3.748 ± 0.056	4.659SerVal: 4.659 ± 0.069	0.894SerTrp: 0.894 ± 0.03	1.268SerTyr: 1.268 ± 0.035	0.0SerXaa: 0.0 ± 0.0
Thr	7.432ThrAla: 7.432 ± 0.08	0.482ThrCys: 0.482 ± 0.019	3.862ThrAsp: 3.862 ± 0.063	3.179ThrGlu: 3.179 ± 0.057	1.979ThrPhe: 1.979 ± 0.041	6.033ThrGly: 6.033 ± 0.071	1.275ThrHis: 1.275 ± 0.031	2.572ThrIle: 2.572 ± 0.045	1.354ThrLys: 1.354 ± 0.038	5.937ThrLeu: 5.937 ± 0.071	1.194ThrMet: 1.194 ± 0.032	1.225ThrAsn: 1.225 ± 0.036	3.979ThrPro: 3.979 ± 0.065	1.525ThrGln: 1.525 ± 0.037	4.017ThrArg: 4.017 ± 0.056	3.857ThrSer: 3.857 ± 0.062	4.333ThrThr: 4.333 ± 0.071	5.482ThrVal: 5.482 ± 0.062	1.053ThrTrp: 1.053 ± 0.032	1.434ThrTyr: 1.434 ± 0.033	0.0ThrXaa: 0.0 ± 0.0
Val	11.913ValAla: 11.913 ± 0.116	0.708ValCys: 0.708 ± 0.025	5.897ValAsp: 5.897 ± 0.061	5.579ValGlu: 5.579 ± 0.074	2.44ValPhe: 2.44 ± 0.049	7.415ValGly: 7.415 ± 0.085	1.964ValHis: 1.964 ± 0.044	3.66ValIle: 3.66 ± 0.053	1.832ValLys: 1.832 ± 0.042	9.565ValLeu: 9.565 ± 0.108	1.65ValMet: 1.65 ± 0.037	1.756ValAsn: 1.756 ± 0.043	4.788ValPro: 4.788 ± 0.064	2.137ValGln: 2.137 ± 0.045	6.509ValArg: 6.509 ± 0.087	4.901ValSer: 4.901 ± 0.063	5.86ValThr: 5.86 ± 0.074	9.646ValVal: 9.646 ± 0.118	1.114ValTrp: 1.114 ± 0.03	1.426ValTyr: 1.426 ± 0.036	0.0ValXaa: 0.0 ± 0.0
Trp	1.63TrpAla: 1.63 ± 0.033	0.156TrpCys: 0.156 ± 0.011	0.859TrpAsp: 0.859 ± 0.026	0.682TrpGlu: 0.682 ± 0.024	0.56TrpPhe: 0.56 ± 0.022	1.027TrpGly: 1.027 ± 0.027	0.375TrpHis: 0.375 ± 0.018	0.58TrpIle: 0.58 ± 0.023	0.307TrpLys: 0.307 ± 0.016	1.854TrpLeu: 1.854 ± 0.042	0.307TrpMet: 0.307 ± 0.017	0.343TrpAsn: 0.343 ± 0.019	0.709TrpPro: 0.709 ± 0.026	0.57TrpGln: 0.57 ± 0.023	1.241TrpArg: 1.241 ± 0.031	1.053TrpSer: 1.053 ± 0.031	0.973TrpThr: 0.973 ± 0.028	1.168TrpVal: 1.168 ± 0.033	0.339TrpTrp: 0.339 ± 0.019	0.253TrpTyr: 0.253 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.517TyrAla: 2.517 ± 0.046	0.166TyrCys: 0.166 ± 0.01	1.607TyrAsp: 1.607 ± 0.032	1.025TyrGlu: 1.025 ± 0.03	0.652TyrPhe: 0.652 ± 0.026	1.854TyrGly: 1.854 ± 0.037	0.329TyrHis: 0.329 ± 0.018	0.541TyrIle: 0.541 ± 0.022	0.415TyrLys: 0.415 ± 0.018	2.018TyrLeu: 2.018 ± 0.048	0.211TyrMet: 0.211 ± 0.016	0.38TyrAsn: 0.38 ± 0.019	0.913TyrPro: 0.913 ± 0.03	0.495TyrGln: 0.495 ± 0.024	1.359TyrArg: 1.359 ± 0.034	0.96TyrSer: 0.96 ± 0.029	1.039TyrThr: 1.039 ± 0.029	1.783TyrVal: 1.783 ± 0.042	0.296TyrTrp: 0.296 ± 0.018	0.449TyrTyr: 0.449 ± 0.018	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3893 proteins (1249285 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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