Streptomyces niveus NCIMB 11891
Average proteome isoelectric point is 6.27
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7746 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|V6KJK0|V6KJK0_STRNV OmpA-like domain-containing protein OS=Streptomyces niveus NCIMB 11891 OX=1352941 GN=M877_06490 PE=4 SV=1
MM1 pKa = 7.34 LTTRR5 pKa = 11.84 YY6 pKa = 10.08 VPGSPNWADD15 pKa = 3.5 LATPDD20 pKa = 4.18 IEE22 pKa = 4.61 GAKK25 pKa = 9.0 TFYY28 pKa = 10.89 GGVLGWDD35 pKa = 3.67 HH36 pKa = 6.64 VPAGPEE42 pKa = 3.6 FGGYY46 pKa = 11.01 GMFQLNGKK54 pKa = 6.64 TVAGVMQLPAEE65 pKa = 4.28 TVPLSWTLYY74 pKa = 9.88 FQTADD79 pKa = 3.21 ATATSSAVKK88 pKa = 10.59 DD89 pKa = 3.51 NGGTVVNEE97 pKa = 3.93 PMDD100 pKa = 3.98 VEE102 pKa = 4.27 DD103 pKa = 4.6 LGRR106 pKa = 11.84 MTLCADD112 pKa = 3.51 PAGAGFATWQPGTNTGLEE130 pKa = 4.18 ATDD133 pKa = 4.17 TPGTLCWAEE142 pKa = 4.85 LYY144 pKa = 10.39 TPDD147 pKa = 3.02 VDD149 pKa = 4.35 AAVGFYY155 pKa = 10.91 GSVFGWEE162 pKa = 4.29 TTTMPMPGGGGDD174 pKa = 3.55 YY175 pKa = 11.46 VMVHH179 pKa = 6.55 PAGKK183 pKa = 10.57 GPDD186 pKa = 3.3 GMFAGVVEE194 pKa = 4.2 NRR196 pKa = 11.84 PDD198 pKa = 3.35 TGLGATGPNWLLYY211 pKa = 10.52 FGTADD216 pKa = 3.75 CDD218 pKa = 3.69 AAVAASDD225 pKa = 3.71 RR226 pKa = 11.84 LGGTTVQEE234 pKa = 4.08 PTDD237 pKa = 3.52 IEE239 pKa = 4.59 GVGRR243 pKa = 11.84 FAVLTDD249 pKa = 3.86 PYY251 pKa = 10.32 GARR254 pKa = 11.84 LAVMQGVSQDD264 pKa = 3.21 TT265 pKa = 3.52
Molecular weight: 27.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.706
IPC2_protein 3.846
IPC_protein 3.846
Toseland 3.63
ProMoST 4.012
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.783
Rodwell 3.668
Grimsley 3.541
Solomon 3.834
Lehninger 3.795
Nozaki 3.961
DTASelect 4.202
Thurlkill 3.681
EMBOSS 3.795
Sillero 3.973
Patrickios 1.1
IPC_peptide 3.834
IPC2_peptide 3.948
IPC2.peptide.svr19 3.862
Protein with the highest isoelectric point:
>tr|V6K2B0|V6K2B0_STRNV Membrane protein OS=Streptomyces niveus NCIMB 11891 OX=1352941 GN=M877_20115 PE=4 SV=1
MM1 pKa = 7.49 HH2 pKa = 7.21 SGVIASFSWAHH13 pKa = 6.57 GDD15 pKa = 3.54 SQAMSRR21 pKa = 11.84 LPNHH25 pKa = 6.51 HH26 pKa = 6.67 LWSPRR31 pKa = 11.84 VSKK34 pKa = 10.08 RR35 pKa = 11.84 TFQPNNRR42 pKa = 11.84 RR43 pKa = 11.84 RR44 pKa = 11.84 AKK46 pKa = 8.7 THH48 pKa = 5.15 GFRR51 pKa = 11.84 LRR53 pKa = 11.84 MRR55 pKa = 11.84 TRR57 pKa = 11.84 AGRR60 pKa = 11.84 AILANRR66 pKa = 11.84 RR67 pKa = 11.84 GKK69 pKa = 10.51 GRR71 pKa = 11.84 ANLSAA76 pKa = 4.66
Molecular weight: 8.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.466
IPC2_protein 11.008
IPC_protein 12.618
Toseland 12.778
ProMoST 13.276
Dawson 12.778
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.369
Grimsley 12.822
Solomon 13.276
Lehninger 13.173
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.276
Sillero 12.778
Patrickios 12.106
IPC_peptide 13.276
IPC2_peptide 12.266
IPC2.peptide.svr19 9.131
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7746
0
7746
2460520
27
5582
317.7
33.99
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.364 ± 0.038
0.731 ± 0.007
6.159 ± 0.025
5.66 ± 0.025
2.736 ± 0.017
9.542 ± 0.028
2.232 ± 0.015
3.263 ± 0.019
2.215 ± 0.025
10.305 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.75 ± 0.011
1.852 ± 0.017
6.046 ± 0.024
2.686 ± 0.016
7.915 ± 0.038
5.229 ± 0.019
6.297 ± 0.026
8.436 ± 0.03
1.501 ± 0.013
2.082 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here