Amino acid dipepetide frequency for Aeromicrobium sp. Root472D3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	18.891AlaAla: 18.891 ± 0.203	0.896AlaCys: 0.896 ± 0.028	9.036AlaAsp: 9.036 ± 0.121	7.357AlaGlu: 7.357 ± 0.095	3.678AlaPhe: 3.678 ± 0.058	11.665AlaGly: 11.665 ± 0.129	2.469AlaHis: 2.469 ± 0.061	5.126AlaIle: 5.126 ± 0.08	2.744AlaLys: 2.744 ± 0.068	12.617AlaLeu: 12.617 ± 0.133	2.878AlaMet: 2.878 ± 0.047	1.939AlaAsn: 1.939 ± 0.042	5.959AlaPro: 5.959 ± 0.109	3.549AlaGln: 3.549 ± 0.063	8.838AlaArg: 8.838 ± 0.105	7.215AlaSer: 7.215 ± 0.087	7.972AlaThr: 7.972 ± 0.102	11.961AlaVal: 11.961 ± 0.121	1.762AlaTrp: 1.762 ± 0.041	2.518AlaTyr: 2.518 ± 0.053	0.001AlaXaa: 0.001 ± 0.001
Cys	0.786CysAla: 0.786 ± 0.024	0.063CysCys: 0.063 ± 0.007	0.426CysAsp: 0.426 ± 0.022	0.335CysGlu: 0.335 ± 0.016	0.189CysPhe: 0.189 ± 0.014	0.755CysGly: 0.755 ± 0.029	0.173CysHis: 0.173 ± 0.011	0.181CysIle: 0.181 ± 0.014	0.105CysLys: 0.105 ± 0.008	0.594CysLeu: 0.594 ± 0.025	0.096CysMet: 0.096 ± 0.011	0.117CysAsn: 0.117 ± 0.009	0.382CysPro: 0.382 ± 0.021	0.14CysGln: 0.14 ± 0.01	0.493CysArg: 0.493 ± 0.024	0.386CysSer: 0.386 ± 0.019	0.407CysThr: 0.407 ± 0.022	0.563CysVal: 0.563 ± 0.024	0.076CysTrp: 0.076 ± 0.009	0.107CysTyr: 0.107 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	8.793AspAla: 8.793 ± 0.091	0.344AspCys: 0.344 ± 0.018	5.95AspAsp: 5.95 ± 0.087	4.96AspGlu: 4.96 ± 0.069	1.676AspPhe: 1.676 ± 0.043	6.886AspGly: 6.886 ± 0.094	1.633AspHis: 1.633 ± 0.036	2.178AspIle: 2.178 ± 0.049	1.182AspLys: 1.182 ± 0.035	7.26AspLeu: 7.26 ± 0.087	0.917AspMet: 0.917 ± 0.028	1.033AspAsn: 1.033 ± 0.028	4.246AspPro: 4.246 ± 0.07	1.736AspGln: 1.736 ± 0.038	5.173AspArg: 5.173 ± 0.079	2.648AspSer: 2.648 ± 0.048	3.18AspThr: 3.18 ± 0.051	7.525AspVal: 7.525 ± 0.089	0.911AspTrp: 0.911 ± 0.033	1.211AspTyr: 1.211 ± 0.039	0.001AspXaa: 0.001 ± 0.001
Glu	6.693GluAla: 6.693 ± 0.092	0.272GluCys: 0.272 ± 0.015	2.509GluAsp: 2.509 ± 0.054	2.297GluGlu: 2.297 ± 0.057	1.507GluPhe: 1.507 ± 0.038	4.097GluGly: 4.097 ± 0.058	1.577GluHis: 1.577 ± 0.043	2.592GluIle: 2.592 ± 0.044	1.366GluLys: 1.366 ± 0.045	6.214GluLeu: 6.214 ± 0.086	0.983GluMet: 0.983 ± 0.029	0.956GluAsn: 0.956 ± 0.032	2.97GluPro: 2.97 ± 0.059	2.293GluGln: 2.293 ± 0.055	4.807GluArg: 4.807 ± 0.078	2.683GluSer: 2.683 ± 0.046	3.05GluThr: 3.05 ± 0.056	4.827GluVal: 4.827 ± 0.072	0.754GluTrp: 0.754 ± 0.03	0.958GluTyr: 0.958 ± 0.029	0.001GluXaa: 0.001 ± 0.001
Phe	3.962PheAla: 3.962 ± 0.057	0.224PheCys: 0.224 ± 0.012	2.351PheAsp: 2.351 ± 0.044	1.62PheGlu: 1.62 ± 0.042	0.931PhePhe: 0.931 ± 0.037	3.159PheGly: 3.159 ± 0.061	0.551PheHis: 0.551 ± 0.021	0.925PheIle: 0.925 ± 0.031	0.549PheLys: 0.549 ± 0.027	2.423PheLeu: 2.423 ± 0.053	0.453PheMet: 0.453 ± 0.023	0.568PheAsn: 0.568 ± 0.025	1.267PhePro: 1.267 ± 0.034	0.642PheGln: 0.642 ± 0.027	1.642PheArg: 1.642 ± 0.04	1.668PheSer: 1.668 ± 0.036	2.003PheThr: 2.003 ± 0.047	3.02PheVal: 3.02 ± 0.048	0.414PheTrp: 0.414 ± 0.023	0.654PheTyr: 0.654 ± 0.024	0.002PheXaa: 0.002 ± 0.001
Gly	10.171GlyAla: 10.171 ± 0.095	0.639GlyCys: 0.639 ± 0.029	5.807GlyAsp: 5.807 ± 0.094	4.564GlyGlu: 4.564 ± 0.066	3.091GlyPhe: 3.091 ± 0.056	8.04GlyGly: 8.04 ± 0.136	2.108GlyHis: 2.108 ± 0.049	3.969GlyIle: 3.969 ± 0.064	2.073GlyLys: 2.073 ± 0.057	9.145GlyLeu: 9.145 ± 0.105	1.872GlyMet: 1.872 ± 0.038	1.599GlyAsn: 1.599 ± 0.035	4.074GlyPro: 4.074 ± 0.062	2.525GlyGln: 2.525 ± 0.056	7.081GlyArg: 7.081 ± 0.093	5.896GlySer: 5.896 ± 0.079	6.146GlyThr: 6.146 ± 0.082	8.241GlyVal: 8.241 ± 0.105	1.56GlyTrp: 1.56 ± 0.04	2.059GlyTyr: 2.059 ± 0.046	0.002GlyXaa: 0.002 ± 0.001
His	2.479HisAla: 2.479 ± 0.049	0.149HisCys: 0.149 ± 0.012	1.776HisAsp: 1.776 ± 0.041	1.274HisGlu: 1.274 ± 0.036	0.559HisPhe: 0.559 ± 0.022	2.181HisGly: 2.181 ± 0.05	0.647HisHis: 0.647 ± 0.025	0.554HisIle: 0.554 ± 0.023	0.282HisLys: 0.282 ± 0.017	2.31HisLeu: 2.31 ± 0.043	0.311HisMet: 0.311 ± 0.016	0.315HisAsn: 0.315 ± 0.017	1.495HisPro: 1.495 ± 0.047	0.584HisGln: 0.584 ± 0.023	1.816HisArg: 1.816 ± 0.047	0.877HisSer: 0.877 ± 0.026	1.082HisThr: 1.082 ± 0.035	2.204HisVal: 2.204 ± 0.047	0.26HisTrp: 0.26 ± 0.016	0.396HisTyr: 0.396 ± 0.02	0.0HisXaa: 0.0 ± 0.0
Ile	5.726IleAla: 5.726 ± 0.076	0.289IleCys: 0.289 ± 0.015	3.268IleAsp: 3.268 ± 0.055	2.487IleGlu: 2.487 ± 0.056	0.914IlePhe: 0.914 ± 0.035	4.528IleGly: 4.528 ± 0.073	0.643IleHis: 0.643 ± 0.023	1.316IleIle: 1.316 ± 0.045	0.83IleLys: 0.83 ± 0.028	2.823IleLeu: 2.823 ± 0.056	0.584IleMet: 0.584 ± 0.027	0.799IleAsn: 0.799 ± 0.029	1.839IlePro: 1.839 ± 0.041	0.8IleGln: 0.8 ± 0.027	2.25IleArg: 2.25 ± 0.04	2.095IleSer: 2.095 ± 0.039	2.356IleThr: 2.356 ± 0.053	4.084IleVal: 4.084 ± 0.074	0.414IleTrp: 0.414 ± 0.02	0.618IleTyr: 0.618 ± 0.029	0.001IleXaa: 0.001 ± 0.001
Lys	2.759LysAla: 2.759 ± 0.064	0.091LysCys: 0.091 ± 0.008	1.367LysAsp: 1.367 ± 0.043	0.942LysGlu: 0.942 ± 0.034	0.478LysPhe: 0.478 ± 0.023	1.875LysGly: 1.875 ± 0.051	0.402LysHis: 0.402 ± 0.019	1.002LysIle: 1.002 ± 0.038	0.967LysLys: 0.967 ± 0.047	1.762LysLeu: 1.762 ± 0.046	0.395LysMet: 0.395 ± 0.021	0.537LysAsn: 0.537 ± 0.026	1.16LysPro: 1.16 ± 0.044	0.7LysGln: 0.7 ± 0.028	1.409LysArg: 1.409 ± 0.04	1.13LysSer: 1.13 ± 0.04	1.345LysThr: 1.345 ± 0.044	2.006LysVal: 2.006 ± 0.057	0.213LysTrp: 0.213 ± 0.014	0.428LysTyr: 0.428 ± 0.022	0.0LysXaa: 0.0 ± 0.0
Leu	13.958LeuAla: 13.958 ± 0.153	0.6LeuCys: 0.6 ± 0.024	7.552LeuAsp: 7.552 ± 0.092	5.107LeuGlu: 5.107 ± 0.09	2.517LeuPhe: 2.517 ± 0.054	8.973LeuGly: 8.973 ± 0.11	1.972LeuHis: 1.972 ± 0.047	3.241LeuIle: 3.241 ± 0.072	1.919LeuLys: 1.919 ± 0.044	9.749LeuLeu: 9.749 ± 0.132	1.699LeuMet: 1.699 ± 0.043	1.518LeuAsn: 1.518 ± 0.043	5.148LeuPro: 5.148 ± 0.073	2.357LeuGln: 2.357 ± 0.051	7.165LeuArg: 7.165 ± 0.1	5.4LeuSer: 5.4 ± 0.069	6.554LeuThr: 6.554 ± 0.075	10.502LeuVal: 10.502 ± 0.116	1.086LeuTrp: 1.086 ± 0.034	1.422LeuTyr: 1.422 ± 0.035	0.0LeuXaa: 0.0 ± 0.0
Met	2.324MetAla: 2.324 ± 0.05	0.108MetCys: 0.108 ± 0.01	0.908MetAsp: 0.908 ± 0.027	0.649MetGlu: 0.649 ± 0.027	0.565MetPhe: 0.565 ± 0.026	1.441MetGly: 1.441 ± 0.036	0.394MetHis: 0.394 ± 0.019	0.804MetIle: 0.804 ± 0.027	0.478MetLys: 0.478 ± 0.022	1.847MetLeu: 1.847 ± 0.041	0.362MetMet: 0.362 ± 0.018	0.407MetAsn: 0.407 ± 0.02	1.063MetPro: 1.063 ± 0.033	0.541MetGln: 0.541 ± 0.023	1.473MetArg: 1.473 ± 0.036	1.488MetSer: 1.488 ± 0.034	1.995MetThr: 1.995 ± 0.041	1.493MetVal: 1.493 ± 0.039	0.188MetTrp: 0.188 ± 0.013	0.302MetTyr: 0.302 ± 0.016	0.0MetXaa: 0.0 ± 0.0
Asn	2.088AsnAla: 2.088 ± 0.052	0.119AsnCys: 0.119 ± 0.01	1.145AsnAsp: 1.145 ± 0.038	0.8AsnGlu: 0.8 ± 0.03	0.526AsnPhe: 0.526 ± 0.024	1.782AsnGly: 1.782 ± 0.042	0.361AsnHis: 0.361 ± 0.017	0.733AsnIle: 0.733 ± 0.029	0.384AsnLys: 0.384 ± 0.024	1.741AsnLeu: 1.741 ± 0.039	0.269AsnMet: 0.269 ± 0.018	0.439AsnAsn: 0.439 ± 0.026	1.263AsnPro: 1.263 ± 0.033	0.477AsnGln: 0.477 ± 0.024	1.214AsnArg: 1.214 ± 0.033	0.808AsnSer: 0.808 ± 0.03	0.97AsnThr: 0.97 ± 0.034	1.602AsnVal: 1.602 ± 0.037	0.228AsnTrp: 0.228 ± 0.015	0.392AsnTyr: 0.392 ± 0.018	0.0AsnXaa: 0.0 ± 0.0
Pro	6.799ProAla: 6.799 ± 0.1	0.256ProCys: 0.256 ± 0.017	4.386ProAsp: 4.386 ± 0.069	3.233ProGlu: 3.233 ± 0.053	1.604ProPhe: 1.604 ± 0.042	4.98ProGly: 4.98 ± 0.076	1.231ProHis: 1.231 ± 0.03	1.72ProIle: 1.72 ± 0.037	1.015ProLys: 1.015 ± 0.034	4.531ProLeu: 4.531 ± 0.069	0.989ProMet: 0.989 ± 0.031	0.804ProAsn: 0.804 ± 0.028	2.35ProPro: 2.35 ± 0.059	1.342ProGln: 1.342 ± 0.035	3.438ProArg: 3.438 ± 0.056	3.321ProSer: 3.321 ± 0.061	3.542ProThr: 3.542 ± 0.07	5.018ProVal: 5.018 ± 0.069	0.873ProTrp: 0.873 ± 0.03	1.021ProTyr: 1.021 ± 0.029	0.001ProXaa: 0.001 ± 0.001
Gln	3.419GlnAla: 3.419 ± 0.051	0.153GlnCys: 0.153 ± 0.011	1.332GlnAsp: 1.332 ± 0.035	1.116GlnGlu: 1.116 ± 0.032	0.73GlnPhe: 0.73 ± 0.024	2.077GlnGly: 2.077 ± 0.042	0.74GlnHis: 0.74 ± 0.023	1.328GlnIle: 1.328 ± 0.041	0.675GlnLys: 0.675 ± 0.027	2.999GlnLeu: 2.999 ± 0.061	0.588GlnMet: 0.588 ± 0.025	0.481GlnAsn: 0.481 ± 0.02	1.415GlnPro: 1.415 ± 0.036	1.168GlnGln: 1.168 ± 0.036	2.318GlnArg: 2.318 ± 0.048	1.34GlnSer: 1.34 ± 0.035	1.572GlnThr: 1.572 ± 0.039	2.734GlnVal: 2.734 ± 0.047	0.468GlnTrp: 0.468 ± 0.022	0.538GlnTyr: 0.538 ± 0.024	0.001GlnXaa: 0.001 ± 0.001
Arg	8.451ArgAla: 8.451 ± 0.121	0.439ArgCys: 0.439 ± 0.023	4.65ArgAsp: 4.65 ± 0.071	3.984ArgGlu: 3.984 ± 0.072	2.277ArgPhe: 2.277 ± 0.048	5.46ArgGly: 5.46 ± 0.078	1.792ArgHis: 1.792 ± 0.046	3.108ArgIle: 3.108 ± 0.058	1.375ArgLys: 1.375 ± 0.04	7.731ArgLeu: 7.731 ± 0.092	1.647ArgMet: 1.647 ± 0.036	1.247ArgAsn: 1.247 ± 0.034	4.123ArgPro: 4.123 ± 0.064	2.116ArgGln: 2.116 ± 0.048	6.822ArgArg: 6.822 ± 0.111	4.45ArgSer: 4.45 ± 0.065	5.001ArgThr: 5.001 ± 0.067	6.06ArgVal: 6.06 ± 0.087	1.266ArgTrp: 1.266 ± 0.035	1.435ArgTyr: 1.435 ± 0.03	0.002ArgXaa: 0.002 ± 0.001
Ser	6.972SerAla: 6.972 ± 0.085	0.387SerCys: 0.387 ± 0.02	3.534SerAsp: 3.534 ± 0.055	2.472SerGlu: 2.472 ± 0.041	1.871SerPhe: 1.871 ± 0.043	5.8SerGly: 5.8 ± 0.073	1.077SerHis: 1.077 ± 0.032	2.411SerIle: 2.411 ± 0.045	1.134SerLys: 1.134 ± 0.037	5.219SerLeu: 5.219 ± 0.073	1.364SerMet: 1.364 ± 0.03	1.026SerAsn: 1.026 ± 0.031	3.047SerPro: 3.047 ± 0.051	1.479SerGln: 1.479 ± 0.034	4.033SerArg: 4.033 ± 0.057	3.81SerSer: 3.81 ± 0.07	4.148SerThr: 4.148 ± 0.075	4.906SerVal: 4.906 ± 0.068	0.908SerTrp: 0.908 ± 0.03	1.329SerTyr: 1.329 ± 0.032	0.003SerXaa: 0.003 ± 0.002
Thr	7.862ThrAla: 7.862 ± 0.093	0.407ThrCys: 0.407 ± 0.022	3.984ThrAsp: 3.984 ± 0.056	2.9ThrGlu: 2.9 ± 0.053	2.177ThrPhe: 2.177 ± 0.051	6.059ThrGly: 6.059 ± 0.088	1.221ThrHis: 1.221 ± 0.037	2.959ThrIle: 2.959 ± 0.066	1.366ThrLys: 1.366 ± 0.043	5.974ThrLeu: 5.974 ± 0.068	1.179ThrMet: 1.179 ± 0.032	1.106ThrAsn: 1.106 ± 0.035	3.96ThrPro: 3.96 ± 0.067	1.477ThrGln: 1.477 ± 0.036	3.907ThrArg: 3.907 ± 0.059	4.443ThrSer: 4.443 ± 0.072	4.8ThrThr: 4.8 ± 0.084	6.414ThrVal: 6.414 ± 0.094	1.075ThrTrp: 1.075 ± 0.032	1.588ThrTyr: 1.588 ± 0.034	0.001ThrXaa: 0.001 ± 0.001
Val	13.026ValAla: 13.026 ± 0.137	0.661ValCys: 0.661 ± 0.022	7.084ValAsp: 7.084 ± 0.082	5.653ValGlu: 5.653 ± 0.078	2.558ValPhe: 2.558 ± 0.048	7.945ValGly: 7.945 ± 0.104	1.906ValHis: 1.906 ± 0.039	3.395ValIle: 3.395 ± 0.051	1.842ValLys: 1.842 ± 0.047	9.955ValLeu: 9.955 ± 0.113	1.71ValMet: 1.71 ± 0.043	1.787ValAsn: 1.787 ± 0.047	5.08ValPro: 5.08 ± 0.074	2.208ValGln: 2.208 ± 0.048	6.918ValArg: 6.918 ± 0.088	5.243ValSer: 5.243 ± 0.067	6.664ValThr: 6.664 ± 0.076	11.585ValVal: 11.585 ± 0.137	1.157ValTrp: 1.157 ± 0.031	1.538ValTyr: 1.538 ± 0.039	0.001ValXaa: 0.001 ± 0.001
Trp	1.479TrpAla: 1.479 ± 0.036	0.137TrpCys: 0.137 ± 0.011	0.873TrpAsp: 0.873 ± 0.031	0.564TrpGlu: 0.564 ± 0.023	0.534TrpPhe: 0.534 ± 0.02	1.012TrpGly: 1.012 ± 0.03	0.352TrpHis: 0.352 ± 0.018	0.614TrpIle: 0.614 ± 0.026	0.317TrpLys: 0.317 ± 0.016	1.582TrpLeu: 1.582 ± 0.043	0.283TrpMet: 0.283 ± 0.016	0.312TrpAsn: 0.312 ± 0.017	0.7TrpPro: 0.7 ± 0.024	0.509TrpGln: 0.509 ± 0.024	1.137TrpArg: 1.137 ± 0.035	0.972TrpSer: 0.972 ± 0.035	1.054TrpThr: 1.054 ± 0.03	1.191TrpVal: 1.191 ± 0.032	0.353TrpTrp: 0.353 ± 0.019	0.267TrpTyr: 0.267 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.432TyrAla: 2.432 ± 0.048	0.141TyrCys: 0.141 ± 0.013	1.508TyrAsp: 1.508 ± 0.035	1.089TyrGlu: 1.089 ± 0.029	0.644TyrPhe: 0.644 ± 0.023	1.948TyrGly: 1.948 ± 0.036	0.303TyrHis: 0.303 ± 0.019	0.507TyrIle: 0.507 ± 0.019	0.365TyrLys: 0.365 ± 0.019	1.962TyrLeu: 1.962 ± 0.042	0.248TyrMet: 0.248 ± 0.014	0.35TyrAsn: 0.35 ± 0.018	0.913TyrPro: 0.913 ± 0.027	0.547TyrGln: 0.547 ± 0.022	1.499TyrArg: 1.499 ± 0.038	1.052TyrSer: 1.052 ± 0.034	1.019TyrThr: 1.019 ± 0.031	1.979TyrVal: 1.979 ± 0.043	0.277TyrTrp: 0.277 ± 0.017	0.4TyrTyr: 0.4 ± 0.018	0.001TyrXaa: 0.001 ± 0.001
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.001	0.002XaaPhe: 0.002 ± 0.001	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.001XaaAsn: 0.001 ± 0.001	0.003XaaPro: 0.003 ± 0.002	0.001XaaGln: 0.001 ± 0.001	0.002XaaArg: 0.002 ± 0.001	0.003XaaSer: 0.003 ± 0.002	0.001XaaThr: 0.001 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.001XaaTrp: 0.001 ± 0.001	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3523 proteins (1115088 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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