Long-fingered bat hepatitis B virus
Average proteome isoelectric point is 8.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|M4PSV1|M4PSV1_9HEPA External core antigen OS=Long-fingered bat hepatitis B virus OX=2050037 GN=C PE=3 SV=1
MM1 pKa = 6.67 GQNWSIPNPLGFLPEE16 pKa = 4.11 HH17 pKa = 6.06 QLPNPYY23 pKa = 10.64 APVYY27 pKa = 10.26 QDD29 pKa = 2.8 WDD31 pKa = 3.94 LNQDD35 pKa = 3.24 KK36 pKa = 10.6 DD37 pKa = 3.28 QWPQARR43 pKa = 11.84 EE44 pKa = 4.02 VSPGAYY50 pKa = 9.78 GLGFVPPHH58 pKa = 6.61 GGLTGNLPFAQEE70 pKa = 4.35 GNLTSNNPEE79 pKa = 4.08 TIVYY83 pKa = 7.86 QIPKK87 pKa = 10.37 AKK89 pKa = 10.3 DD90 pKa = 3.17 EE91 pKa = 4.43 SQTQVIQLPAPPRR104 pKa = 11.84 TTTNRR109 pKa = 11.84 KK110 pKa = 9.38 KK111 pKa = 10.63 GRR113 pKa = 11.84 QPTPPTPPVRR123 pKa = 11.84 VTHH126 pKa = 6.2 PHH128 pKa = 6.5 LNMPSWRR135 pKa = 11.84 EE136 pKa = 3.88 KK137 pKa = 10.16 YY138 pKa = 10.45 GHH140 pKa = 7.76 LITAGGSSSKK150 pKa = 10.71 LQIPAVTAASVTSSASSTIGDD171 pKa = 4.15 PATDD175 pKa = 3.34 MGITTSGFLVPLAGLQVGCFLLTKK199 pKa = 9.8 ILEE202 pKa = 4.36 IGKK205 pKa = 9.55 SLDD208 pKa = 3.21 WWWTSLSFPGGIPVCIGQNSQSQTSSHH235 pKa = 6.51 SPTSCPPTCTGFRR248 pKa = 11.84 WMWLRR253 pKa = 11.84 RR254 pKa = 11.84 FIIYY258 pKa = 10.48 LLVLLLCAIFLLVLLDD274 pKa = 3.37 WRR276 pKa = 11.84 GFLPVCPLPTSSSTTTRR293 pKa = 11.84 CSTCTATATDD303 pKa = 5.72 PITWPYY309 pKa = 9.41 CCCSKK314 pKa = 9.6 PTDD317 pKa = 4.21 GNCTCWPIPTSWALGKK333 pKa = 9.6 FLWGWASARR342 pKa = 11.84 FSWLNSLLPWLLWFAEE358 pKa = 4.29 LSPTVWLLLIWMMWFWGPSLLTIFEE383 pKa = 4.43 PFIPLCALFFVIWEE397 pKa = 4.24 LWW399 pKa = 3.15
Molecular weight: 44.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.905
IPC2_protein 6.927
IPC_protein 6.854
Toseland 6.532
ProMoST 7.38
Dawson 7.512
Bjellqvist 7.907
Wikipedia 7.424
Rodwell 7.527
Grimsley 6.561
Solomon 7.556
Lehninger 7.585
Nozaki 8.287
DTASelect 7.702
Thurlkill 7.746
EMBOSS 7.761
Sillero 8.097
Patrickios 3.401
IPC_peptide 7.556
IPC2_peptide 7.497
IPC2.peptide.svr19 7.236
Protein with the highest isoelectric point:
>tr|M4Q3B7|M4Q3B7_9HEPA Protein X OS=Long-fingered bat hepatitis B virus OX=2050037 GN=X PE=3 SV=1
MM1 pKa = 7.69 AARR4 pKa = 11.84 LRR6 pKa = 11.84 CQLDD10 pKa = 3.04 ASGNVVLLRR19 pKa = 11.84 PLCIEE24 pKa = 3.86 SGGRR28 pKa = 11.84 PVARR32 pKa = 11.84 SARR35 pKa = 11.84 HH36 pKa = 4.68 SRR38 pKa = 11.84 RR39 pKa = 11.84 AAASPVPTVHH49 pKa = 6.48 GPHH52 pKa = 4.82 VTLRR56 pKa = 11.84 RR57 pKa = 11.84 LPICAASPAGPCALRR72 pKa = 11.84 FTCANFSGCMEE83 pKa = 3.98 TTMNFVTWLGTRR95 pKa = 11.84 RR96 pKa = 11.84 RR97 pKa = 11.84 TGAKK101 pKa = 9.76 LNSGHH106 pKa = 6.22 WDD108 pKa = 3.18 MYY110 pKa = 10.64 FRR112 pKa = 11.84 EE113 pKa = 4.34 SLMKK117 pKa = 10.13 EE118 pKa = 3.78 WEE120 pKa = 4.29 EE121 pKa = 4.01 KK122 pKa = 11.29 GNDD125 pKa = 3.25 QRR127 pKa = 11.84 LYY129 pKa = 8.51 TYY131 pKa = 10.93 VSGGCRR137 pKa = 11.84 HH138 pKa = 6.57 KK139 pKa = 10.92 LLCTLL144 pKa = 4.45
Molecular weight: 15.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.309
IPC2_protein 9.194
IPC_protein 9.575
Toseland 9.926
ProMoST 9.75
Dawson 10.16
Bjellqvist 10.014
Wikipedia 10.365
Rodwell 10.292
Grimsley 10.218
Solomon 10.248
Lehninger 10.218
Nozaki 10.218
DTASelect 9.911
Thurlkill 10.043
EMBOSS 10.365
Sillero 10.175
Patrickios 9.882
IPC_peptide 10.233
IPC2_peptide 9.619
IPC2.peptide.svr19 8.022
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1613
144
853
403.3
45.13
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.448 ± 0.547
3.038 ± 0.522
2.914 ± 0.186
2.976 ± 0.651
4.278 ± 0.204
6.696 ± 0.515
3.658 ± 0.948
3.968 ± 0.526
3.472 ± 0.94
11.903 ± 0.448
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.674 ± 0.244
3.472 ± 0.325
9.423 ± 0.769
3.658 ± 0.369
6.696 ± 1.559
8.555 ± 0.24
6.944 ± 1.149
4.65 ± 0.158
3.348 ± 0.903
2.232 ± 0.251
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here