Duck adenovirus 1
Average proteome isoelectric point is 6.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 29 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|O11413|O11413_9ADEN IVa2 protein OS=Duck adenovirus 1 OX=130329 GN=IVa2 PE=4 SV=1
MM1 pKa = 7.93 AFRR4 pKa = 11.84 PAPPPAPFTAFPYY17 pKa = 9.6 LTGNLPTWSVSTVKK31 pKa = 10.44 NDD33 pKa = 3.2 PSMPLGAYY41 pKa = 10.57 LMLPSEE47 pKa = 4.26 NPEE50 pKa = 4.05 DD51 pKa = 3.91 VFNKK55 pKa = 9.52 YY56 pKa = 7.0 QTVYY60 pKa = 10.6 LVPGEE65 pKa = 4.07 QYY67 pKa = 9.77 RR68 pKa = 11.84 WKK70 pKa = 10.47 QVIIKK75 pKa = 10.33 SSITIHH81 pKa = 5.84 GQGALVKK88 pKa = 10.25 LDD90 pKa = 3.71 GPGPSLTISGGSGLPIDD107 pKa = 4.05 MTVEE111 pKa = 3.56 LRR113 pKa = 11.84 DD114 pKa = 3.65 INFKK118 pKa = 11.02 GLDD121 pKa = 3.37 IPPDD125 pKa = 3.63 RR126 pKa = 11.84 EE127 pKa = 4.07 EE128 pKa = 4.25 LMEE131 pKa = 4.79 DD132 pKa = 2.78 AFLRR136 pKa = 11.84 HH137 pKa = 5.37 SAIWSHH143 pKa = 5.2 NVTRR147 pKa = 11.84 TVINGCSFVNFKK159 pKa = 10.38 GAAVWFYY166 pKa = 11.53 DD167 pKa = 5.14 EE168 pKa = 4.44 DD169 pKa = 4.39 TTWSGRR175 pKa = 11.84 IFAEE179 pKa = 3.37 QHH181 pKa = 6.02 LFINNRR187 pKa = 11.84 ITGCRR192 pKa = 11.84 IGLALGSVSRR202 pKa = 11.84 NSVASHH208 pKa = 6.85 NIFNDD213 pKa = 3.15 CHH215 pKa = 7.03 VCFNVLGGGWTLVNNCIMNSRR236 pKa = 11.84 CAYY239 pKa = 9.33 LHH241 pKa = 6.52 AKK243 pKa = 9.21 EE244 pKa = 4.34 GMWYY248 pKa = 10.16 SGPATSGPALVNVFSGNVLNNCAANGNMWPTDD280 pKa = 3.64 YY281 pKa = 11.23 LGGGDD286 pKa = 4.25 VPLKK290 pKa = 10.56 LAAFYY295 pKa = 10.7 FDD297 pKa = 5.81 DD298 pKa = 4.66 EE299 pKa = 4.64 DD300 pKa = 5.56 AIPPVWTGNAHH311 pKa = 5.38 NWADD315 pKa = 3.42 VTLTKK320 pKa = 10.55 FSSDD324 pKa = 3.33 LEE326 pKa = 4.6 SYY328 pKa = 10.86 SITGCMFIGQTTAVPSAGRR347 pKa = 11.84 IYY349 pKa = 10.83 VGNTISDD356 pKa = 3.2 KK357 pKa = 11.26 VYY359 pKa = 10.61 FFGCSGNGVYY369 pKa = 10.58 LYY371 pKa = 9.14 GTTDD375 pKa = 3.39 ANLFPDD381 pKa = 4.81 DD382 pKa = 4.5 FGTAQSGNPPSS393 pKa = 3.43
Molecular weight: 42.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.782
IPC2_protein 4.991
IPC_protein 4.952
Toseland 4.902
ProMoST 5.13
Dawson 4.991
Bjellqvist 5.105
Wikipedia 4.902
Rodwell 4.889
Grimsley 4.838
Solomon 4.991
Lehninger 4.952
Nozaki 5.118
DTASelect 5.334
Thurlkill 4.94
EMBOSS 4.965
Sillero 5.181
Patrickios 2.079
IPC_peptide 4.991
IPC2_peptide 5.169
IPC2.peptide.svr19 5.057
Protein with the highest isoelectric point:
>tr|O11420|O11420_9ADEN PVI protein OS=Duck adenovirus 1 OX=130329 GN=pVI PE=4 SV=1
MM1 pKa = 7.46 RR2 pKa = 11.84 RR3 pKa = 11.84 SRR5 pKa = 11.84 SYY7 pKa = 9.91 GGLRR11 pKa = 11.84 YY12 pKa = 8.69 GHH14 pKa = 5.63 SVVRR18 pKa = 11.84 YY19 pKa = 8.22 RR20 pKa = 11.84 RR21 pKa = 11.84 SSQVRR26 pKa = 11.84 ARR28 pKa = 11.84 RR29 pKa = 11.84 PRR31 pKa = 11.84 RR32 pKa = 11.84 RR33 pKa = 11.84 LKK35 pKa = 10.99 GGFLPAIIPLIAAAISAAPAIAGTVIAAKK64 pKa = 10.16 NARR67 pKa = 3.7
Molecular weight: 7.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.31
IPC2_protein 11.052
IPC_protein 12.252
Toseland 12.398
ProMoST 12.91
Dawson 12.398
Bjellqvist 12.398
Wikipedia 12.881
Rodwell 11.989
Grimsley 12.442
Solomon 12.896
Lehninger 12.793
Nozaki 12.398
DTASelect 12.398
Thurlkill 12.398
EMBOSS 12.896
Sillero 12.398
Patrickios 11.711
IPC_peptide 12.91
IPC2_peptide 11.886
IPC2.peptide.svr19 9.095
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
29
0
29
9891
67
1079
341.1
38.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.451 ± 0.497
2.396 ± 0.43
4.802 ± 0.345
5.136 ± 0.396
4.459 ± 0.255
5.732 ± 0.549
2.204 ± 0.216
5.49 ± 0.288
4.671 ± 0.557
9.837 ± 0.396
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.022 ± 0.179
5.348 ± 0.403
6.036 ± 0.302
3.923 ± 0.278
5.763 ± 0.599
6.541 ± 0.235
6.855 ± 0.452
6.066 ± 0.376
1.415 ± 0.15
3.852 ± 0.302
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here