Dragonfly larvae associated circular virus-10
Average proteome isoelectric point is 7.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W5U2Q8|W5U2Q8_9VIRU Putative capsid protein OS=Dragonfly larvae associated circular virus-10 OX=1454022 PE=4 SV=1
MM1 pKa = 7.72 PKK3 pKa = 10.08 VKK5 pKa = 10.17 QGSQGEE11 pKa = 4.38 RR12 pKa = 11.84 WCFTLNNYY20 pKa = 7.23 TEE22 pKa = 4.87 KK23 pKa = 10.79 DD24 pKa = 3.6 VASITEE30 pKa = 4.15 KK31 pKa = 10.4 LTFSNCVFAKK41 pKa = 9.42 VGKK44 pKa = 9.98 EE45 pKa = 3.62 IGDD48 pKa = 3.45 SGTPHH53 pKa = 6.26 LQGFIHH59 pKa = 6.22 LRR61 pKa = 11.84 KK62 pKa = 9.6 RR63 pKa = 11.84 LRR65 pKa = 11.84 LACLKK70 pKa = 10.76 KK71 pKa = 10.62 LVGDD75 pKa = 4.08 RR76 pKa = 11.84 AHH78 pKa = 6.95 CEE80 pKa = 3.91 VARR83 pKa = 11.84 GTDD86 pKa = 3.33 KK87 pKa = 11.49 DD88 pKa = 3.73 NEE90 pKa = 4.68 SYY92 pKa = 10.6 CGKK95 pKa = 10.47 DD96 pKa = 2.83 SDD98 pKa = 4.25 VVLTIGEE105 pKa = 4.24 PTIGTTEE112 pKa = 3.8 QGGGDD117 pKa = 4.2 TIHH120 pKa = 6.98 SIARR124 pKa = 11.84 RR125 pKa = 11.84 IALKK129 pKa = 10.4 LSKK132 pKa = 10.22 GTDD135 pKa = 2.86 ITEE138 pKa = 4.21 LQGEE142 pKa = 4.31 EE143 pKa = 3.32 WKK145 pKa = 10.68 AYY147 pKa = 9.19 CRR149 pKa = 11.84 HH150 pKa = 5.88 SKK152 pKa = 10.43 VIQEE156 pKa = 4.14 LSAALVKK163 pKa = 10.81 NKK165 pKa = 10.2 NIKK168 pKa = 9.91 DD169 pKa = 3.5 QAAQMSDD176 pKa = 2.82 KK177 pKa = 10.67 PLRR180 pKa = 11.84 TWQKK184 pKa = 8.35 EE185 pKa = 4.06 LKK187 pKa = 10.29 NAIQTVPDD195 pKa = 3.64 DD196 pKa = 4.44 RR197 pKa = 11.84 KK198 pKa = 11.06 VMWYY202 pKa = 9.56 CDD204 pKa = 3.57 SVGNTGKK211 pKa = 7.88 TWFSKK216 pKa = 10.97 YY217 pKa = 9.94 LVALHH222 pKa = 5.77 GAIRR226 pKa = 11.84 FEE228 pKa = 4.3 NGKK231 pKa = 9.72 SADD234 pKa = 2.81 IKK236 pKa = 10.1 YY237 pKa = 9.96 AYY239 pKa = 9.99 NGEE242 pKa = 4.14 RR243 pKa = 11.84 VVVFDD248 pKa = 4.9 LSRR251 pKa = 11.84 SQVDD255 pKa = 3.12 HH256 pKa = 6.69 FNYY259 pKa = 10.21 EE260 pKa = 4.09 VIEE263 pKa = 4.41 SIKK266 pKa = 10.44 NGLMFSPKK274 pKa = 8.87 YY275 pKa = 8.71 TSCTKK280 pKa = 9.95 MYY282 pKa = 8.29 PIPHH286 pKa = 6.39 VIVFANWMSDD296 pKa = 3.3 EE297 pKa = 4.67 SKK299 pKa = 10.9 LSADD303 pKa = 2.99 RR304 pKa = 11.84 WKK306 pKa = 10.5 IEE308 pKa = 4.03 SLSDD312 pKa = 3.25 VSKK315 pKa = 10.81 IKK317 pKa = 10.55 CEE319 pKa = 4.1 KK320 pKa = 10.01 EE321 pKa = 3.42 DD322 pKa = 3.78 DD323 pKa = 4.32 VIVNDD328 pKa = 4.1 VIVIEE333 pKa = 5.01 DD334 pKa = 4.34 DD335 pKa = 5.02 DD336 pKa = 4.3 VQQEE340 pKa = 4.27 QIDD343 pKa = 4.44 SYY345 pKa = 11.4 FSNLFDD351 pKa = 3.43 NWGPDD356 pKa = 3.47 CVGDD360 pKa = 3.83 LDD362 pKa = 4.73 EE363 pKa = 4.52 YY364 pKa = 11.15 QQ365 pKa = 3.36
Molecular weight: 41.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.581
IPC2_protein 5.601
IPC_protein 5.614
Toseland 5.842
ProMoST 5.893
Dawson 5.804
Bjellqvist 5.842
Wikipedia 5.779
Rodwell 5.766
Grimsley 5.919
Solomon 5.804
Lehninger 5.779
Nozaki 6.033
DTASelect 6.224
Thurlkill 6.16
EMBOSS 6.122
Sillero 6.122
Patrickios 4.202
IPC_peptide 5.817
IPC2_peptide 6.097
IPC2.peptide.svr19 6.187
Protein with the highest isoelectric point:
>tr|W5U2Q8|W5U2Q8_9VIRU Putative capsid protein OS=Dragonfly larvae associated circular virus-10 OX=1454022 PE=4 SV=1
MM1 pKa = 7.18 IKK3 pKa = 10.0 AYY5 pKa = 10.0 RR6 pKa = 11.84 VGRR9 pKa = 11.84 EE10 pKa = 4.02 RR11 pKa = 11.84 IATTQLSAVTMAPYY25 pKa = 10.67 RR26 pKa = 11.84 NLTRR30 pKa = 11.84 GRR32 pKa = 11.84 YY33 pKa = 6.6 SAKK36 pKa = 9.3 RR37 pKa = 11.84 RR38 pKa = 11.84 YY39 pKa = 7.24 RR40 pKa = 11.84 TRR42 pKa = 11.84 RR43 pKa = 11.84 VTRR46 pKa = 11.84 KK47 pKa = 9.09 RR48 pKa = 11.84 YY49 pKa = 9.49 ARR51 pKa = 11.84 TRR53 pKa = 11.84 FSKK56 pKa = 10.52 RR57 pKa = 11.84 RR58 pKa = 11.84 FGHH61 pKa = 6.65 RR62 pKa = 11.84 NRR64 pKa = 11.84 SWAMRR69 pKa = 11.84 KK70 pKa = 9.69 RR71 pKa = 11.84 ISNRR75 pKa = 11.84 RR76 pKa = 11.84 RR77 pKa = 11.84 SGTKK81 pKa = 8.36 VLKK84 pKa = 10.3 VVHH87 pKa = 6.79 RR88 pKa = 11.84 WTIDD92 pKa = 2.89 KK93 pKa = 10.93 SFEE96 pKa = 3.74 NGIYY100 pKa = 9.79 TDD102 pKa = 4.25 CLSMNASDD110 pKa = 4.55 VNNLPGWAQLTVLYY124 pKa = 10.55 DD125 pKa = 3.07 EE126 pKa = 4.9 WKK128 pKa = 10.1 PGKK131 pKa = 10.19 KK132 pKa = 9.77 YY133 pKa = 9.87 MKK135 pKa = 10.34 SFVPFDD141 pKa = 3.32 KK142 pKa = 11.4 YY143 pKa = 11.25 EE144 pKa = 4.29 NEE146 pKa = 4.13 VSDD149 pKa = 3.79 NAEE152 pKa = 4.33 LIVRR156 pKa = 11.84 WSAYY160 pKa = 10.6 DD161 pKa = 3.49 PDD163 pKa = 3.99 AKK165 pKa = 10.61 GRR167 pKa = 11.84 KK168 pKa = 7.07 FTAARR173 pKa = 11.84 AVSDD177 pKa = 3.73 MEE179 pKa = 4.78 KK180 pKa = 9.23 MQNSKK185 pKa = 9.5 WKK187 pKa = 10.19 LIKK190 pKa = 10.29 KK191 pKa = 8.09 NQVVSTSYY199 pKa = 11.12 QPMFPTRR206 pKa = 11.84 TGVNLTGVKK215 pKa = 10.4 GVDD218 pKa = 3.6 NPWRR222 pKa = 11.84 DD223 pKa = 3.53 CVASVTSEE231 pKa = 3.68 QCQNGVQQVWKK242 pKa = 10.48 VNTPTTLVTRR252 pKa = 11.84 HH253 pKa = 5.95 YY254 pKa = 7.86 FTQVYY259 pKa = 7.88 YY260 pKa = 10.9 FRR262 pKa = 11.84 GLRR265 pKa = 11.84 IGTQYY270 pKa = 10.39 GAQYY274 pKa = 10.81 EE275 pKa = 4.55 PVTPAFPEE283 pKa = 4.17 VVPPLKK289 pKa = 10.57 KK290 pKa = 9.22 VDD292 pKa = 3.48 RR293 pKa = 11.84 MRR295 pKa = 11.84 TEE297 pKa = 3.5
Molecular weight: 34.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.321
IPC2_protein 9.926
IPC_protein 10.672
Toseland 10.716
ProMoST 10.394
Dawson 10.833
Bjellqvist 10.526
Wikipedia 11.038
Rodwell 11.125
Grimsley 10.891
Solomon 10.906
Lehninger 10.877
Nozaki 10.687
DTASelect 10.526
Thurlkill 10.716
EMBOSS 11.111
Sillero 10.745
Patrickios 10.804
IPC_peptide 10.921
IPC2_peptide 9.428
IPC2.peptide.svr19 8.425
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
662
297
365
331.0
38.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.589 ± 0.315
1.964 ± 0.638
6.495 ± 1.642
5.287 ± 1.059
3.323 ± 0.029
5.891 ± 0.562
1.662 ± 0.436
4.834 ± 1.431
8.761 ± 0.455
5.589 ± 0.811
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.266 ± 0.511
4.683 ± 0.246
3.474 ± 0.829
4.079 ± 0.026
7.553 ± 2.83
6.798 ± 0.493
6.647 ± 1.184
8.459 ± 0.648
2.417 ± 0.185
4.23 ± 0.774
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here