Salmonella phage BP12C
Average proteome isoelectric point is 6.21
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A140XFV9|A0A140XFV9_9CAUD Uncharacterized protein OS=Salmonella phage BP12C OX=1543203 GN=BP12C_04 PE=4 SV=1
MM1 pKa = 7.41 AGLWFFVVTICGAVGADD18 pKa = 4.09 NMAADD23 pKa = 3.87 CHH25 pKa = 7.17 DD26 pKa = 3.9 YY27 pKa = 11.38 VIDD30 pKa = 4.27 AGISFDD36 pKa = 3.69 DD37 pKa = 4.16 CRR39 pKa = 11.84 ASVAAYY45 pKa = 8.11 PGRR48 pKa = 11.84 IGLYY52 pKa = 8.3 SVRR55 pKa = 11.84 CDD57 pKa = 2.75 RR58 pKa = 11.84 GEE60 pKa = 3.97 VVEE63 pKa = 4.71 GGNHH67 pKa = 4.72 EE68 pKa = 4.2
Molecular weight: 7.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.128
IPC2_protein 4.355
IPC_protein 4.215
Toseland 4.024
ProMoST 4.291
Dawson 4.215
Bjellqvist 4.469
Wikipedia 4.164
Rodwell 4.05
Grimsley 3.935
Solomon 4.202
Lehninger 4.151
Nozaki 4.342
DTASelect 4.584
Thurlkill 4.088
EMBOSS 4.177
Sillero 4.342
Patrickios 0.54
IPC_peptide 4.19
IPC2_peptide 4.317
IPC2.peptide.svr19 4.295
Protein with the highest isoelectric point:
>tr|A0A140XFV7|A0A140XFV7_9CAUD Uncharacterized protein OS=Salmonella phage BP12C OX=1543203 GN=BP12C_02 PE=4 SV=1
MM1 pKa = 8.11 DD2 pKa = 4.65 KK3 pKa = 10.86 AQRR6 pKa = 11.84 LTAARR11 pKa = 11.84 MAADD15 pKa = 4.68 RR16 pKa = 11.84 YY17 pKa = 10.94 AGIARR22 pKa = 11.84 AKK24 pKa = 9.77 GFKK27 pKa = 9.78 RR28 pKa = 11.84 HH29 pKa = 6.12 ADD31 pKa = 3.01 GVTFTRR37 pKa = 11.84 ADD39 pKa = 4.17 ADD41 pKa = 4.01 LTWDD45 pKa = 3.3 DD46 pKa = 3.23 RR47 pKa = 11.84 ARR49 pKa = 11.84 AFRR52 pKa = 11.84 VTLYY56 pKa = 11.25 KK57 pKa = 9.69 MDD59 pKa = 3.61 GAARR63 pKa = 11.84 LAVATVRR70 pKa = 11.84 ANAMLNVLLKK80 pKa = 11.08 AFII83 pKa = 4.46
Molecular weight: 9.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.315
IPC2_protein 9.809
IPC_protein 10.877
Toseland 11.038
ProMoST 11.052
Dawson 11.096
Bjellqvist 10.891
Wikipedia 11.389
Rodwell 11.169
Grimsley 11.125
Solomon 11.359
Lehninger 11.301
Nozaki 11.023
DTASelect 10.891
Thurlkill 11.038
EMBOSS 11.477
Sillero 11.038
Patrickios 10.935
IPC_peptide 11.359
IPC2_peptide 9.984
IPC2.peptide.svr19 8.701
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
76
0
76
19108
51
1431
251.4
27.99
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.855 ± 0.473
0.984 ± 0.108
6.803 ± 0.237
6.202 ± 0.29
3.47 ± 0.148
7.144 ± 0.297
1.669 ± 0.143
5.584 ± 0.136
5.16 ± 0.311
7.269 ± 0.273
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.067 ± 0.128
4.286 ± 0.196
4.637 ± 0.417
3.836 ± 0.33
5.961 ± 0.226
5.417 ± 0.199
6.259 ± 0.296
7.117 ± 0.265
1.764 ± 0.144
3.517 ± 0.201
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here