Chitinophaga niastensis
Average proteome isoelectric point is 6.93
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5788 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2P8HHD0|A0A2P8HHD0_9BACT YkoY family integral membrane protein OS=Chitinophaga niastensis OX=536980 GN=CLV51_104331 PE=4 SV=1
MM1 pKa = 7.17 QIAVRR6 pKa = 11.84 DD7 pKa = 3.69 QLEE10 pKa = 4.18 PLLQDD15 pKa = 3.0 MLYY18 pKa = 9.79 ISEE21 pKa = 4.36 SEE23 pKa = 4.39 YY24 pKa = 10.63 PLEE27 pKa = 4.02 LLNLGTYY34 pKa = 9.59 TDD36 pKa = 4.29 DD37 pKa = 3.47 AALYY41 pKa = 8.36 STVSQEE47 pKa = 3.66 TSIPFDD53 pKa = 3.37 GAVVKK58 pKa = 10.52 EE59 pKa = 4.05 DD60 pKa = 3.27 ADD62 pKa = 3.49 IFFEE66 pKa = 4.23 EE67 pKa = 5.03 TISNLINGGDD77 pKa = 3.8 SVSAGLGKK85 pKa = 10.35 KK86 pKa = 9.81 YY87 pKa = 9.59 EE88 pKa = 3.88 QLYY91 pKa = 10.99 AFVQSTFPEE100 pKa = 4.03 RR101 pKa = 11.84 WVSRR105 pKa = 11.84 VTIGAEE111 pKa = 3.85 VNICIIGITADD122 pKa = 3.84 NEE124 pKa = 4.19 CSVIKK129 pKa = 9.72 TLAIEE134 pKa = 4.21 TT135 pKa = 4.15
Molecular weight: 14.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.776
IPC2_protein 3.923
IPC_protein 3.846
Toseland 3.656
ProMoST 3.986
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.706
Rodwell 3.681
Grimsley 3.567
Solomon 3.795
Lehninger 3.745
Nozaki 3.935
DTASelect 4.075
Thurlkill 3.694
EMBOSS 3.719
Sillero 3.961
Patrickios 1.863
IPC_peptide 3.795
IPC2_peptide 3.935
IPC2.peptide.svr19 3.862
Protein with the highest isoelectric point:
>tr|A0A2P8HBY1|A0A2P8HBY1_9BACT Putative secreted protein (Por secretion system target) OS=Chitinophaga niastensis OX=536980 GN=CLV51_10744 PE=4 SV=1
MM1 pKa = 7.69 PKK3 pKa = 10.34 VKK5 pKa = 8.9 THH7 pKa = 5.39 SRR9 pKa = 11.84 AKK11 pKa = 9.49 KK12 pKa = 7.15 TFKK15 pKa = 10.87 VGGNGQIKK23 pKa = 10.14 RR24 pKa = 11.84 FMAFKK29 pKa = 10.7 SHH31 pKa = 7.17 LLTKK35 pKa = 10.44 KK36 pKa = 7.53 STKK39 pKa = 9.68 RR40 pKa = 11.84 KK41 pKa = 8.54 RR42 pKa = 11.84 SLRR45 pKa = 11.84 GSTLVHH51 pKa = 6.05 SANLNLVKK59 pKa = 10.61 RR60 pKa = 11.84 MLGMRR65 pKa = 4.28
Molecular weight: 7.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.473
IPC2_protein 11.096
IPC_protein 12.618
Toseland 12.808
ProMoST 13.29
Dawson 12.808
Bjellqvist 12.793
Wikipedia 13.276
Rodwell 12.749
Grimsley 12.852
Solomon 13.29
Lehninger 13.188
Nozaki 12.808
DTASelect 12.793
Thurlkill 12.808
EMBOSS 13.29
Sillero 12.808
Patrickios 12.486
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.033
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5788
0
5788
2177340
27
9035
376.2
42.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.054 ± 0.04
0.866 ± 0.011
5.196 ± 0.022
5.162 ± 0.042
4.576 ± 0.026
6.972 ± 0.034
2.072 ± 0.02
6.93 ± 0.029
6.287 ± 0.039
9.567 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.421 ± 0.019
5.412 ± 0.044
4.144 ± 0.022
4.138 ± 0.025
4.037 ± 0.023
6.101 ± 0.029
6.17 ± 0.051
6.434 ± 0.032
1.278 ± 0.012
4.181 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here