Chitinophaga niastensis

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Chitinophagia; Chitinophagales; Chitinophagaceae; Chitinophaga

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5788 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2P8HHD0|A0A2P8HHD0_9BACT YkoY family integral membrane protein OS=Chitinophaga niastensis OX=536980 GN=CLV51_104331 PE=4 SV=1
MM1 pKa = 7.17QIAVRR6 pKa = 11.84DD7 pKa = 3.69QLEE10 pKa = 4.18PLLQDD15 pKa = 3.0MLYY18 pKa = 9.79ISEE21 pKa = 4.36SEE23 pKa = 4.39YY24 pKa = 10.63PLEE27 pKa = 4.02LLNLGTYY34 pKa = 9.59TDD36 pKa = 4.29DD37 pKa = 3.47AALYY41 pKa = 8.36STVSQEE47 pKa = 3.66TSIPFDD53 pKa = 3.37GAVVKK58 pKa = 10.52EE59 pKa = 4.05DD60 pKa = 3.27ADD62 pKa = 3.49IFFEE66 pKa = 4.23EE67 pKa = 5.03TISNLINGGDD77 pKa = 3.8SVSAGLGKK85 pKa = 10.35KK86 pKa = 9.81YY87 pKa = 9.59EE88 pKa = 3.88QLYY91 pKa = 10.99AFVQSTFPEE100 pKa = 4.03RR101 pKa = 11.84WVSRR105 pKa = 11.84VTIGAEE111 pKa = 3.85VNICIIGITADD122 pKa = 3.84NEE124 pKa = 4.19CSVIKK129 pKa = 9.72TLAIEE134 pKa = 4.21TT135 pKa = 4.15

Molecular weight:
14.88 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2P8HBY1|A0A2P8HBY1_9BACT Putative secreted protein (Por secretion system target) OS=Chitinophaga niastensis OX=536980 GN=CLV51_10744 PE=4 SV=1
MM1 pKa = 7.69PKK3 pKa = 10.34VKK5 pKa = 8.9THH7 pKa = 5.39SRR9 pKa = 11.84AKK11 pKa = 9.49KK12 pKa = 7.15TFKK15 pKa = 10.87VGGNGQIKK23 pKa = 10.14RR24 pKa = 11.84FMAFKK29 pKa = 10.7SHH31 pKa = 7.17LLTKK35 pKa = 10.44KK36 pKa = 7.53STKK39 pKa = 9.68RR40 pKa = 11.84KK41 pKa = 8.54RR42 pKa = 11.84SLRR45 pKa = 11.84GSTLVHH51 pKa = 6.05SANLNLVKK59 pKa = 10.61RR60 pKa = 11.84MLGMRR65 pKa = 4.28

Molecular weight:
7.42 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5788

0

5788

2177340

27

9035

376.2

42.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.054 ± 0.04

0.866 ± 0.011

5.196 ± 0.022

5.162 ± 0.042

4.576 ± 0.026

6.972 ± 0.034

2.072 ± 0.02

6.93 ± 0.029

6.287 ± 0.039

9.567 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.421 ± 0.019

5.412 ± 0.044

4.144 ± 0.022

4.138 ± 0.025

4.037 ± 0.023

6.101 ± 0.029

6.17 ± 0.051

6.434 ± 0.032

1.278 ± 0.012

4.181 ± 0.026

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski