Methanosphaera sp. rholeuAM74
Average proteome isoelectric point is 5.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1765 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A328SIU9|A0A328SIU9_9EURY 50S ribosomal protein L24e OS=Methanosphaera sp. rholeuAM74 OX=1945579 GN=rpl24e PE=3 SV=1
MM1 pKa = 7.52 NGRR4 pKa = 11.84 MHH6 pKa = 7.56 DD7 pKa = 3.91 KK8 pKa = 10.57 NIKK11 pKa = 9.33 TMFLLTVLFILLVSATSISASNNTTTDD38 pKa = 3.23 DD39 pKa = 4.02 AMNNTNVSTSIPIKK53 pKa = 10.72 SNVSMDD59 pKa = 3.28 SKK61 pKa = 11.67 ANQTNNTKK69 pKa = 10.31 IIANTSNHH77 pKa = 5.96 SNLSKK82 pKa = 9.82 TEE84 pKa = 4.04 RR85 pKa = 11.84 TVKK88 pKa = 10.28 SYY90 pKa = 11.1 PDD92 pKa = 3.47 YY93 pKa = 11.28 DD94 pKa = 3.52 PYY96 pKa = 11.58 GNKK99 pKa = 9.58 EE100 pKa = 3.97 VLISFTDD107 pKa = 3.13 IFDD110 pKa = 4.0 GDD112 pKa = 3.79 VIYY115 pKa = 10.03 PHH117 pKa = 7.25 DD118 pKa = 4.72 RR119 pKa = 11.84 PLSIKK124 pKa = 10.74 LSTTYY129 pKa = 11.29 DD130 pKa = 3.23 DD131 pKa = 4.05 GVYY134 pKa = 10.78 GNRR137 pKa = 11.84 EE138 pKa = 3.64 HH139 pKa = 7.64 PFRR142 pKa = 11.84 GNLTVTINDD151 pKa = 3.27 NQTFIIKK158 pKa = 10.43 GSRR161 pKa = 11.84 NCEE164 pKa = 4.03 FIFNPTTEE172 pKa = 4.07 GLQNIKK178 pKa = 10.6 VAVLDD183 pKa = 4.33 SSDD186 pKa = 3.28 EE187 pKa = 4.2 PYY189 pKa = 10.84 YY190 pKa = 11.31 GEE192 pKa = 4.47 GDD194 pKa = 3.59 VSFTYY199 pKa = 10.84 YY200 pKa = 10.68 RR201 pKa = 11.84 FDD203 pKa = 3.4 TRR205 pKa = 11.84 IVNLDD210 pKa = 3.38 DD211 pKa = 3.6 TATGHH216 pKa = 6.23 VDD218 pKa = 3.0 EE219 pKa = 5.46 ALNISSYY226 pKa = 10.76 LQYY229 pKa = 11.52 DD230 pKa = 4.23 DD231 pKa = 5.11 IYY233 pKa = 9.94 TGFVNFEE240 pKa = 4.15 DD241 pKa = 4.28 EE242 pKa = 5.12 ANVTLQINDD251 pKa = 3.53 EE252 pKa = 4.47 VIPLLVEE259 pKa = 4.69 DD260 pKa = 5.82 GIISYY265 pKa = 10.7 NYY267 pKa = 8.04 TPQSVGTYY275 pKa = 10.28 NITLTYY281 pKa = 10.72 GGDD284 pKa = 3.43 SYY286 pKa = 11.46 FRR288 pKa = 11.84 PSNKK292 pKa = 8.92 SWNYY296 pKa = 8.18 TVNPYY301 pKa = 8.02 EE302 pKa = 4.18 TEE304 pKa = 4.05 LVIDD308 pKa = 5.14 NIDD311 pKa = 3.88 DD312 pKa = 3.89 FDD314 pKa = 3.82 NTVYY318 pKa = 10.98 VDD320 pKa = 3.91 DD321 pKa = 4.51 GVVFNLTLRR330 pKa = 11.84 DD331 pKa = 3.61 SRR333 pKa = 11.84 TKK335 pKa = 10.48 KK336 pKa = 10.41 AIPNAEE342 pKa = 3.29 IRR344 pKa = 11.84 VRR346 pKa = 11.84 HH347 pKa = 6.42 DD348 pKa = 3.08 SDD350 pKa = 3.99 EE351 pKa = 4.2 YY352 pKa = 11.25 TITTDD357 pKa = 3.75 DD358 pKa = 4.21 GGHH361 pKa = 5.22 VSITNFSTTAGLHH374 pKa = 5.91 TIDD377 pKa = 4.56 FKK379 pKa = 11.84 YY380 pKa = 10.65 DD381 pKa = 3.03 GSAIATPSNNTLSFEE396 pKa = 4.12 VSKK399 pKa = 11.1 RR400 pKa = 11.84 DD401 pKa = 4.43 LEE403 pKa = 4.3 LTLDD407 pKa = 5.0 DD408 pKa = 4.33 IDD410 pKa = 5.17 SVTLYY415 pKa = 10.71 SQALIQGDD423 pKa = 4.08 VLNLSGNDD431 pKa = 3.44 DD432 pKa = 3.95 VEE434 pKa = 4.5 VVIVIDD440 pKa = 3.97 DD441 pKa = 3.77 QNYY444 pKa = 6.96 TASFTNQTHH453 pKa = 5.36 YY454 pKa = 10.89 SYY456 pKa = 11.29 YY457 pKa = 8.83 YY458 pKa = 8.37 TALSVGTHH466 pKa = 5.13 NVSVYY471 pKa = 8.71 TVEE474 pKa = 3.9 NYY476 pKa = 10.36 KK477 pKa = 10.42 YY478 pKa = 10.23 LQSNVVNSSFTVEE491 pKa = 5.06 KK492 pKa = 10.12 IKK494 pKa = 9.39 TWIHH498 pKa = 6.42 LDD500 pKa = 3.36 DD501 pKa = 4.03 STFSGIRR508 pKa = 11.84 DD509 pKa = 3.72 FSVDD513 pKa = 4.11 DD514 pKa = 4.55 SIKK517 pKa = 10.38 MIAQLEE523 pKa = 4.42 TGDD526 pKa = 4.99 DD527 pKa = 4.01 EE528 pKa = 4.89 EE529 pKa = 6.32 DD530 pKa = 3.35 SGNILLFNITGDD542 pKa = 3.99 GISDD546 pKa = 4.25 DD547 pKa = 3.84 EE548 pKa = 4.36 LSYY551 pKa = 11.52 NVVRR555 pKa = 11.84 NANWYY560 pKa = 9.28 HH561 pKa = 5.98 IQFTMIPAGEE571 pKa = 4.13 YY572 pKa = 10.73 NITAYY577 pKa = 9.52 TRR579 pKa = 11.84 DD580 pKa = 3.71 TDD582 pKa = 3.33 NYY584 pKa = 8.24 MYY586 pKa = 11.25 SMNSTIITVHH596 pKa = 6.72 KK597 pKa = 9.39 GDD599 pKa = 3.66 VMIDD603 pKa = 3.2 ASLSEE608 pKa = 4.79 DD609 pKa = 3.72 SVALYY614 pKa = 11.23 SNITLDD620 pKa = 3.65 VSLEE624 pKa = 3.94 DD625 pKa = 3.91 SEE627 pKa = 5.39 GEE629 pKa = 4.06 KK630 pKa = 10.61 LVNHH634 pKa = 6.79 PFVIEE639 pKa = 3.96 VDD641 pKa = 3.49 GQTFDD646 pKa = 3.54 TKK648 pKa = 10.55 TGEE651 pKa = 4.46 DD652 pKa = 3.44 GLATFTYY659 pKa = 8.46 QTQHH663 pKa = 5.85 TGNTMVTVRR672 pKa = 11.84 VDD674 pKa = 3.11 EE675 pKa = 4.53 SYY677 pKa = 11.16 SYY679 pKa = 11.58 YY680 pKa = 10.75 EE681 pKa = 3.87 STRR684 pKa = 11.84 NLTFNTYY691 pKa = 10.57 LLNTVIEE698 pKa = 4.51 VNATDD703 pKa = 4.46 SIVGKK708 pKa = 10.28 NSTITVTLLDD718 pKa = 3.88 EE719 pKa = 5.59 LSNKK723 pKa = 9.93 LNNQTLMIEE732 pKa = 4.53 VNGQTSTVTTDD743 pKa = 3.19 DD744 pKa = 3.51 TGCAVILYY752 pKa = 6.63 PTTSVGLNNVTVTFNPTTKK771 pKa = 10.82 YY772 pKa = 11.43 NMTSTTTQFNVYY784 pKa = 7.52 RR785 pKa = 11.84 HH786 pKa = 4.97 KK787 pKa = 9.87 TIINLTTTDD796 pKa = 2.76 IKK798 pKa = 10.97 INQTEE803 pKa = 4.27 VLSGSLSDD811 pKa = 4.59 EE812 pKa = 4.68 DD813 pKa = 5.33 GEE815 pKa = 4.32 PLANALVDD823 pKa = 4.06 LSVNGDD829 pKa = 3.74 VIIIPTDD836 pKa = 3.11 EE837 pKa = 4.45 LGRR840 pKa = 11.84 YY841 pKa = 8.0 SYY843 pKa = 11.06 EE844 pKa = 4.07 YY845 pKa = 10.39 IPVNSGMHH853 pKa = 5.18 TVTVDD858 pKa = 3.9 FYY860 pKa = 11.65 EE861 pKa = 4.84 DD862 pKa = 4.13 DD863 pKa = 4.0 YY864 pKa = 12.1 YY865 pKa = 11.83 NDD867 pKa = 3.77 SKK869 pKa = 11.83 VEE871 pKa = 3.97 DD872 pKa = 3.8 SFNVTKK878 pKa = 10.85 YY879 pKa = 9.22 DD880 pKa = 3.34 TTLTVDD886 pKa = 3.69 YY887 pKa = 10.51 TPITYY892 pKa = 9.85 VQDD895 pKa = 3.48 SARR898 pKa = 11.84 TSIQGILTDD907 pKa = 3.71 EE908 pKa = 4.78 LEE910 pKa = 4.63 NPVTAAKK917 pKa = 9.89 IKK919 pKa = 10.58 LVIDD923 pKa = 3.96 GGEE926 pKa = 3.91 HH927 pKa = 6.66 DD928 pKa = 4.85 VYY930 pKa = 10.82 TIEE933 pKa = 5.08 DD934 pKa = 3.14 GSYY937 pKa = 8.8 QYY939 pKa = 11.27 SYY941 pKa = 9.46 KK942 pKa = 8.54 TTVNGTKK949 pKa = 10.17 SVTAVYY955 pKa = 10.48 GGDD958 pKa = 3.16 NYY960 pKa = 11.35 YY961 pKa = 11.0 NPSKK965 pKa = 10.66 DD966 pKa = 3.47 VQTSFSVTFSTPDD979 pKa = 3.23 DD980 pKa = 4.01 TPVDD984 pKa = 3.4 NHH986 pKa = 6.64 TDD988 pKa = 3.95 DD989 pKa = 5.6 NPTTNNTPATNQTNTTTDD1007 pKa = 3.33 NNSSEE1012 pKa = 4.08 GSDD1015 pKa = 3.39 VNINVTNGTSEE1026 pKa = 4.12 NNNVSSNISDD1036 pKa = 3.3 VTANHH1041 pKa = 6.78 TNTTTNNTNTTANVTNTTSNMTNTTQSDD1069 pKa = 4.17 TNTTGNATNIDD1080 pKa = 3.39 INATNNTNVNNTNVTGNSSDD1100 pKa = 3.35 VDD1102 pKa = 4.09 VNSTTDD1108 pKa = 3.38 TNITDD1113 pKa = 3.53 SDD1115 pKa = 4.13 VTGNASDD1122 pKa = 4.36 VDD1124 pKa = 4.24 VNDD1127 pKa = 3.92 TNGTIVDD1134 pKa = 3.86 NSNVTGNSSGDD1145 pKa = 3.78 VVNGTSDD1152 pKa = 3.68 TNITDD1157 pKa = 3.89 SNITGNGSGVVTNNTDD1173 pKa = 3.14 NTTVNATNVTGNTSEE1188 pKa = 4.29 VVTNGTNGTSVDD1200 pKa = 3.55 NSNVTGNSSGDD1211 pKa = 3.69 VVNGSSDD1218 pKa = 3.69 TNITDD1223 pKa = 3.9 SNITGNSSGVVTNNTNSTAVNDD1245 pKa = 4.04 TNMTGNSTDD1254 pKa = 3.64 VIVNGTNGTNITDD1267 pKa = 3.75 SNITGNSTDD1276 pKa = 3.17 VVTNSTNATGVDD1288 pKa = 3.5 NSNVTGNSSGVVSNDD1303 pKa = 2.97 TGGTNITNSTITGNTTNTTEE1323 pKa = 3.84 NSANTTIEE1331 pKa = 3.84 NSNITTNNTNSTINQNNTNITNVTINDD1358 pKa = 3.64 TSSNNTVNSTNSTHH1372 pKa = 5.35 TNEE1375 pKa = 5.0 NITNNTVNNTTIDD1388 pKa = 3.35 SSTEE1392 pKa = 3.68 NGTQPISGNVTPNATDD1408 pKa = 3.36 VPSNVTNNTTVVDD1421 pKa = 4.21 SNDD1424 pKa = 3.22 TKK1426 pKa = 11.01 TSDD1429 pKa = 3.82 EE1430 pKa = 4.66 NITGNTSPPDD1440 pKa = 3.83 AQNSTSDD1447 pKa = 3.11 QTDD1450 pKa = 3.27 IEE1452 pKa = 4.42 NSTVAEE1458 pKa = 4.13 NEE1460 pKa = 4.16 TLNNTNVTSNEE1471 pKa = 4.07 TIEE1474 pKa = 4.63 DD1475 pKa = 4.02 SNVTEE1480 pKa = 5.37 DD1481 pKa = 3.17 VTANDD1486 pKa = 3.84 TVNNTNVTEE1495 pKa = 4.1 NDD1497 pKa = 3.58 TVVDD1501 pKa = 4.08 SNVTEE1506 pKa = 4.07 NATVNDD1512 pKa = 4.0 TVDD1515 pKa = 3.66 YY1516 pKa = 11.13 TNVTSNDD1523 pKa = 3.63 TIEE1526 pKa = 4.77 DD1527 pKa = 3.95 SNVTEE1532 pKa = 4.16 NVTVNDD1538 pKa = 4.05 TVNNTDD1544 pKa = 3.07 VTVNGTVEE1552 pKa = 4.03 NSNVTEE1558 pKa = 4.67 DD1559 pKa = 3.29 VTANDD1564 pKa = 4.25 SVNSTNVTEE1573 pKa = 4.3 NVTVVDD1579 pKa = 4.12 SNVTEE1584 pKa = 4.09 NVTVNDD1590 pKa = 3.92 TVNNTNVTTNVTIEE1604 pKa = 4.31 DD1605 pKa = 4.15 SNVTTNVTVNDD1616 pKa = 4.11 TVNSSEE1622 pKa = 4.17 NVTINQTTNVTVGDD1636 pKa = 3.77 NSSVVNQSSTDD1647 pKa = 3.47 VNNNADD1653 pKa = 3.78 MNNDD1657 pKa = 3.5 RR1658 pKa = 11.84 QHH1660 pKa = 6.69 EE1661 pKa = 4.17 DD1662 pKa = 3.25 SSDD1665 pKa = 3.63 SEE1667 pKa = 4.86 AGDD1670 pKa = 3.95 GSDD1673 pKa = 5.1 VSDD1676 pKa = 5.5 DD1677 pKa = 4.4 NINTNPVDD1685 pKa = 3.64 VSGGDD1690 pKa = 3.5 NPSGNPSSDD1699 pKa = 3.29 DD1700 pKa = 3.54 TQSEE1704 pKa = 4.64 DD1705 pKa = 3.54 NSNDD1709 pKa = 3.31 NPEE1712 pKa = 3.98 SSEE1715 pKa = 4.19 TQEE1718 pKa = 4.95 SEE1720 pKa = 4.71 SNPNPSDD1727 pKa = 3.7 SQDD1730 pKa = 3.22 VSSVSDD1736 pKa = 3.32 NSQPVSDD1743 pKa = 4.07 VASVRR1748 pKa = 11.84 SSVASTASSGVVMASVADD1766 pKa = 3.73 VASYY1770 pKa = 11.03 AIVDD1774 pKa = 4.18 SVVSDD1779 pKa = 3.74 AAVEE1783 pKa = 4.3 TVDD1786 pKa = 3.31 KK1787 pKa = 10.74 KK1788 pKa = 11.06 AYY1790 pKa = 9.06 EE1791 pKa = 4.15 LSEE1794 pKa = 4.02 MDD1796 pKa = 4.53 TFFDD1800 pKa = 4.82 DD1801 pKa = 4.53 LKK1803 pKa = 11.45 NFAKK1807 pKa = 10.62 YY1808 pKa = 10.07 FIFIFGCIGIGYY1820 pKa = 6.44 TYY1822 pKa = 11.22 KK1823 pKa = 10.09 MSKK1826 pKa = 10.14
Molecular weight: 196.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.677
IPC2_protein 3.656
IPC_protein 3.719
Toseland 3.478
ProMoST 3.884
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.656
Rodwell 3.528
Grimsley 3.376
Solomon 3.719
Lehninger 3.668
Nozaki 3.821
DTASelect 4.101
Thurlkill 3.528
EMBOSS 3.668
Sillero 3.834
Patrickios 1.227
IPC_peptide 3.706
IPC2_peptide 3.821
IPC2.peptide.svr19 3.768
Protein with the highest isoelectric point:
>tr|A0A328S9L7|A0A328S9L7_9EURY Adhesin OS=Methanosphaera sp. rholeuAM74 OX=1945579 GN=BZ136_01360 PE=4 SV=1
MM1 pKa = 7.63 SKK3 pKa = 10.67 NKK5 pKa = 9.58 QLPKK9 pKa = 10.39 KK10 pKa = 9.96 IRR12 pKa = 11.84 LAKK15 pKa = 9.02 KK16 pKa = 7.29 TKK18 pKa = 7.69 QNRR21 pKa = 11.84 RR22 pKa = 11.84 VPIFAMMKK30 pKa = 7.54 TSRR33 pKa = 11.84 RR34 pKa = 11.84 LRR36 pKa = 11.84 THH38 pKa = 6.3 PKK40 pKa = 8.25 ARR42 pKa = 11.84 QWSRR46 pKa = 11.84 SKK48 pKa = 10.88 IKK50 pKa = 10.61 VV51 pKa = 3.14
Molecular weight: 6.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.492
IPC2_protein 11.14
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.749
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.486
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.066
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1765
0
1765
508127
25
3292
287.9
32.29
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.417 ± 0.069
1.15 ± 0.027
6.611 ± 0.071
6.574 ± 0.089
3.503 ± 0.043
6.016 ± 0.068
1.643 ± 0.029
9.122 ± 0.077
7.023 ± 0.074
8.206 ± 0.074
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.255 ± 0.038
7.51 ± 0.154
2.891 ± 0.04
2.748 ± 0.046
3.275 ± 0.054
6.534 ± 0.064
7.514 ± 0.131
6.916 ± 0.082
0.58 ± 0.017
4.482 ± 0.053
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here